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The map label for this gene is dagK [H]

Identifier: 23098222

GI number: 23098222

Start: 819417

End: 820334

Strand: Direct

Name: dagK [H]

Synonym: OB0767

Alternate gene names: 23098222

Gene position: 819417-820334 (Clockwise)

Preceding gene: 23098221

Following gene: 23098223

Centisome position: 22.57

GC content: 36.82

Gene sequence:

>918_bases
ATGAAAAAGGCCCGAATCATCTATAATCCAACTTCGGGACGCGAAGCAATAAAAAGAGCTTTACCGAATATACTTGAAAA
ATTAGAAGTTGCTGGTTTTGAGACATCCACACATGCCACAACATGTGAAGGAGATGCTACGGAAGCTGCAAAAATAGCTG
TCGAACGTAGATATGATTTAGTTATCGCGGCCGGGGGAGACGGAACGATTAATGAAGTAATAAACGGACTAGCAGAACAA
GAACACCGTCCGCAATTAGGAATAATCCCAGCTGGTACTACGAATGACTTTGCACGAGCTCTACATATTCCTCGTGATAT
TGACAAAGCAGTAGATGTCATTATTGAAGGAAATAGGATGAAGCTTGACATTGGTAGAGTCAATGATGACCATTATTTTA
TCAATATTGCTGGTGGAGGAAAATTAACGGAATTAACCTACGAAGTTCCAAGCAAATTAAAAACACTCCTTGGTCAGCTA
GCTTACTATATAAAAGGTATTGAGATGCTCCCATTCTTGAAAGCAACAAGAGTGAAGATTGAATATGATGATCAGGTCCT
AGAAGATGATATTATGCTGTTTCTTATTTCCAATACAAACTCTGTAGGAGGATTTGAGAAGCTTGCTCCAGATGCAAAAT
TAAATGATGGATACTTCGATCTTCTCATACTAAAGAAAACAAATCTTGCAGAATTTATTCAAATCGCTACTTTAGCTTTA
CGTGGAGATCATTTTAAAAGTAAAAACATTATCTATACACAAGCAAAGCACATAAAAGTATCGAATGAAGAAAAAATGCA
ATTAAATATTGATGGAGAGTATGGCGGGTTACTCCCGGGAGAATTTTTAAATCTACGGCAGCATATCGAATTTTATGTTC
CTAAGGAATTTATAAAAGCACAGGAAGACTTGGATTAA

Upstream 100 bases:

>100_bases
AAGAAACCTAGGAGATACTTCTTCAATAAGTAAAACACCCAAGGGTTCTCCGATAATATGATAAAGTTTCTATCTGTTGA
ACGTATGGGGAGGAAATAAC

Downstream 100 bases:

>100_bases
AAGTCTTCCTTTTGTATGTTTAGAAGGATAAGAAAGATGGGATTCTATTCTTGAGACGGCGCTTATGGTAGAATAATTAT
TTAAGACGAATATCGTAATA

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKKARIIYNPTSGREAIKRALPNILEKLEVAGFETSTHATTCEGDATEAAKIAVERRYDLVIAAGGDGTINEVINGLAEQ
EHRPQLGIIPAGTTNDFARALHIPRDIDKAVDVIIEGNRMKLDIGRVNDDHYFINIAGGGKLTELTYEVPSKLKTLLGQL
AYYIKGIEMLPFLKATRVKIEYDDQVLEDDIMLFLISNTNSVGGFEKLAPDAKLNDGYFDLLILKKTNLAEFIQIATLAL
RGDHFKSKNIIYTQAKHIKVSNEEKMQLNIDGEYGGLLPGEFLNLRQHIEFYVPKEFIKAQEDLD

Sequences:

>Translated_305_residues
MKKARIIYNPTSGREAIKRALPNILEKLEVAGFETSTHATTCEGDATEAAKIAVERRYDLVIAAGGDGTINEVINGLAEQ
EHRPQLGIIPAGTTNDFARALHIPRDIDKAVDVIIEGNRMKLDIGRVNDDHYFINIAGGGKLTELTYEVPSKLKTLLGQL
AYYIKGIEMLPFLKATRVKIEYDDQVLEDDIMLFLISNTNSVGGFEKLAPDAKLNDGYFDLLILKKTNLAEFIQIATLAL
RGDHFKSKNIIYTQAKHIKVSNEEKMQLNIDGEYGGLLPGEFLNLRQHIEFYVPKEFIKAQEDLD
>Mature_305_residues
MKKARIIYNPTSGREAIKRALPNILEKLEVAGFETSTHATTCEGDATEAAKIAVERRYDLVIAAGGDGTINEVINGLAEQ
EHRPQLGIIPAGTTNDFARALHIPRDIDKAVDVIIEGNRMKLDIGRVNDDHYFINIAGGGKLTELTYEVPSKLKTLLGQL
AYYIKGIEMLPFLKATRVKIEYDDQVLEDDIMLFLISNTNSVGGFEKLAPDAKLNDGYFDLLILKKTNLAEFIQIATLAL
RGDHFKSKNIIYTQAKHIKVSNEEKMQLNIDGEYGGLLPGEFLNLRQHIEFYVPKEFIKAQEDLD

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=244, Percent_Identity=34.8360655737705, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 34077; Mature: 34077

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKARIIYNPTSGREAIKRALPNILEKLEVAGFETSTHATTCEGDATEAAKIAVERRYDL
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHEEEEEEEE
VIAAGGDGTINEVINGLAEQEHRPQLGIIPAGTTNDFARALHIPRDIDKAVDVIIEGNRM
EEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHHHHHEEECCCEE
KLDIGRVNDDHYFINIAGGGKLTELTYEVPSKLKTLLGQLAYYIKGIEMLPFLKATRVKI
EEEEEEECCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEEE
EYDDQVLEDDIMLFLISNTNSVGGFEKLAPDAKLNDGYFDLLILKKTNLAEFIQIATLAL
EECCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHE
RGDHFKSKNIIYTQAKHIKVSNEEKMQLNIDGEYGGLLPGEFLNLRQHIEFYVPKEFIKA
ECCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHEEECCHHHHHH
QEDLD
HHCCC
>Mature Secondary Structure
MKKARIIYNPTSGREAIKRALPNILEKLEVAGFETSTHATTCEGDATEAAKIAVERRYDL
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHEEEEEEEE
VIAAGGDGTINEVINGLAEQEHRPQLGIIPAGTTNDFARALHIPRDIDKAVDVIIEGNRM
EEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHHHHHEEECCCEE
KLDIGRVNDDHYFINIAGGGKLTELTYEVPSKLKTLLGQLAYYIKGIEMLPFLKATRVKI
EEEEEEECCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEEE
EYDDQVLEDDIMLFLISNTNSVGGFEKLAPDAKLNDGYFDLLILKKTNLAEFIQIATLAL
EECCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHE
RGDHFKSKNIIYTQAKHIKVSNEEKMQLNIDGEYGGLLPGEFLNLRQHIEFYVPKEFIKA
ECCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHEEECCHHHHHH
QEDLD
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]