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The map label for this gene is purH

Identifier: 23098204

GI number: 23098204

Start: 796822

End: 798354

Strand: Direct

Name: purH

Synonym: OB0749

Alternate gene names: 23098204

Gene position: 796822-798354 (Clockwise)

Preceding gene: 23098203

Following gene: 23098205

Centisome position: 21.95

GC content: 39.92

Gene sequence:

>1533_bases
ATGAGTAAACGTGCGTTAATTAGTGTGTCAGATAAAACAAATATAATCGAATTTGCCAAAGGTCTCAAAGAAAGTGGATT
CGAAATCTTATCTACAGGTGGGACATTAAGAAGTATAGCTGAAGCGGGAATCGATGTAACACCAGTAGATGAAGTAACTG
GATTCCCAGAGATGTTAGATGGACGTGTGAAAACGTTGCATCCAATGATTCATGGGGGATTACTTGGTAAACGTTCCAAC
CACGAGCACCTTAGCCAAATGGAAGAGCATGGCATCAGATCAATCGATCTAGTAGCAGTTAATCTTTATCCGTTTAAAGA
GACTGTGCAAAAACCGGACGTAAGCCATCAAGATATTATCGAGAATATAGATATTGGCGGTCCATCGATGTTACGCTCTG
CTGCAAAGAACTTTGAAGATGTACTAGTTGTAACGGGTCCTACAGATTACAATCGTGTGCTAGCAGCAATTACATCTGAA
ACAGATACATATGAATTTCGTCAGCAATTAGCAGCAAAAGTATTTCGACATACTGCTAGCTATGATGCGATGATAGCGAA
TTACTTCCTATCTCAGACAGAAGAGCAATATCCAGAAAGTTATACAGTTACGTATGAAAAAGTGCAAGATTTACGTTATG
GAGAAAATCCACATCAGCAAGCCGCATTTTATAAAGAACCAATTCAATCTCGTCCTACATTAGCAACAGCAAAGCAACTT
CATGGTAAGGAGCTTTCTTATAACAACATTCAAGATACGAATGCAGCGATAGAAATTGTAAAAGAGTTTGCAGAACCTGC
TGCAGTCGCAGTAAAACACATGAATCCTTGTGGAATAGGAATTGGCGAATCGATTTCAACTGCATTTGAGCGGGCATATC
AAGCAGATCCAACCTCGATATTCGGAGGAATTGTAGCGTGTAATCGACCGGTGGATGCGGCTACTGCAGAACAATTAAGT
CAGATATTCTTAGAAATTGTTGTGGCACCATCCTTTGAATCACAAGCGCTAGAAATTTTAACTCAAAAGAAAAATATCCG
ACTATTGGAGTTAGATGTTACGAGTGATAATAAACAATCCAATCGTTTAACAACAGTAGATGGTGGCGCATTAATCCAAG
CTTATGATGCAAAAGAAGTAAGTGAAGCGGACCTTGAGGTAGTCACCAATAAACAACCTACGGAACAAGAAATCAATGAT
ATGTTGTTTGCTTGGAAAGCAGTAAAACATGTGAAGTCAAATGCAATTGTCTTAGCTAAAGACTCACAAACAATTGGTGT
TGGTGCGGGTCAGATGAATCGTATTGGAGCTGCAGAGATTGCTATTAAGCAAGCGGGAGATAAAAGTGAAGGAGCTGTTT
TAGCTTCAGACGCATTCTTCCCGATGCCAGATACGGTGGAAGCTGCTGCTAAAGCTGGTATTAAGGCAATCATTCAGCCT
GGAGGATCAAAGCGAGACCAAGATTCGGTTGATGTATGTAACCAATTTGGGATTGCTATGGTTTATACAAAGGTAAGACA
CTTTAAGCACTAA

Upstream 100 bases:

>100_bases
ATACAAAAGAAAAATTAAAAGAACGTATACAGAAGGTAGAACATCAACTTTATCCAACAGTAATTAATCAAGTATTGTCT
AATAAAGTGAGGTAATAACA

Downstream 100 bases:

>100_bases
TAGTAGGAGGGACGCTCTTGAACGTTTTAGTAGTCGGCCGAGGCGGCAGAGAACATACCATTGTAAAAAAACTGAAAGCA
AATACAAAAATTGTAAATCT

Product: IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MSKRALISVSDKTNIIEFAKGLKESGFEILSTGGTLRSIAEAGIDVTPVDEVTGFPEMLDGRVKTLHPMIHGGLLGKRSN
HEHLSQMEEHGIRSIDLVAVNLYPFKETVQKPDVSHQDIIENIDIGGPSMLRSAAKNFEDVLVVTGPTDYNRVLAAITSE
TDTYEFRQQLAAKVFRHTASYDAMIANYFLSQTEEQYPESYTVTYEKVQDLRYGENPHQQAAFYKEPIQSRPTLATAKQL
HGKELSYNNIQDTNAAIEIVKEFAEPAAVAVKHMNPCGIGIGESISTAFERAYQADPTSIFGGIVACNRPVDAATAEQLS
QIFLEIVVAPSFESQALEILTQKKNIRLLELDVTSDNKQSNRLTTVDGGALIQAYDAKEVSEADLEVVTNKQPTEQEIND
MLFAWKAVKHVKSNAIVLAKDSQTIGVGAGQMNRIGAAEIAIKQAGDKSEGAVLASDAFFPMPDTVEAAAKAGIKAIIQP
GGSKRDQDSVDVCNQFGIAMVYTKVRHFKH

Sequences:

>Translated_510_residues
MSKRALISVSDKTNIIEFAKGLKESGFEILSTGGTLRSIAEAGIDVTPVDEVTGFPEMLDGRVKTLHPMIHGGLLGKRSN
HEHLSQMEEHGIRSIDLVAVNLYPFKETVQKPDVSHQDIIENIDIGGPSMLRSAAKNFEDVLVVTGPTDYNRVLAAITSE
TDTYEFRQQLAAKVFRHTASYDAMIANYFLSQTEEQYPESYTVTYEKVQDLRYGENPHQQAAFYKEPIQSRPTLATAKQL
HGKELSYNNIQDTNAAIEIVKEFAEPAAVAVKHMNPCGIGIGESISTAFERAYQADPTSIFGGIVACNRPVDAATAEQLS
QIFLEIVVAPSFESQALEILTQKKNIRLLELDVTSDNKQSNRLTTVDGGALIQAYDAKEVSEADLEVVTNKQPTEQEIND
MLFAWKAVKHVKSNAIVLAKDSQTIGVGAGQMNRIGAAEIAIKQAGDKSEGAVLASDAFFPMPDTVEAAAKAGIKAIIQP
GGSKRDQDSVDVCNQFGIAMVYTKVRHFKH
>Mature_509_residues
SKRALISVSDKTNIIEFAKGLKESGFEILSTGGTLRSIAEAGIDVTPVDEVTGFPEMLDGRVKTLHPMIHGGLLGKRSNH
EHLSQMEEHGIRSIDLVAVNLYPFKETVQKPDVSHQDIIENIDIGGPSMLRSAAKNFEDVLVVTGPTDYNRVLAAITSET
DTYEFRQQLAAKVFRHTASYDAMIANYFLSQTEEQYPESYTVTYEKVQDLRYGENPHQQAAFYKEPIQSRPTLATAKQLH
GKELSYNNIQDTNAAIEIVKEFAEPAAVAVKHMNPCGIGIGESISTAFERAYQADPTSIFGGIVACNRPVDAATAEQLSQ
IFLEIVVAPSFESQALEILTQKKNIRLLELDVTSDNKQSNRLTTVDGGALIQAYDAKEVSEADLEVVTNKQPTEQEINDM
LFAWKAVKHVKSNAIVLAKDSQTIGVGAGQMNRIGAAEIAIKQAGDKSEGAVLASDAFFPMPDTVEAAAKAGIKAIIQPG
GSKRDQDSVDVCNQFGIAMVYTKVRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=476, Percent_Identity=37.8151260504202, Blast_Score=264, Evalue=2e-70,
Organism=Escherichia coli, GI1790439, Length=528, Percent_Identity=51.1363636363636, Blast_Score=524, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI71985564, Length=486, Percent_Identity=36.2139917695473, Blast_Score=255, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI71985574, Length=323, Percent_Identity=29.4117647058824, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323768, Length=503, Percent_Identity=35.5864811133201, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6323056, Length=489, Percent_Identity=35.3783231083845, Blast_Score=256, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24649832, Length=505, Percent_Identity=36.6336633663366, Blast_Score=276, Evalue=3e-74,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_OCEIH (Q8CXK7)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691670.1
- ProteinModelPortal:   Q8CXK7
- SMR:   Q8CXK7
- GeneID:   1016275
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0749
- NMPDR:   fig|221109.1.peg.757
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- BioCyc:   OIHE221109:OB0749-MONOMER
- BRENDA:   2.1.2.3
- BRENDA:   3.5.4.10
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55748; Mature: 55616

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRALISVSDKTNIIEFAKGLKESGFEILSTGGTLRSIAEAGIDVTPVDEVTGFPEMLD
CCCCEEEEECCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCHHHHCCCHHHHC
GRVKTLHPMIHGGLLGKRSNHEHLSQMEEHGIRSIDLVAVNLYPFKETVQKPDVSHQDII
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCHHHHCCCCCCHHHHH
ENIDIGGPSMLRSAAKNFEDVLVVTGPTDYNRVLAAITSETDTYEFRQQLAAKVFRHTAS
HHCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YDAMIANYFLSQTEEQYPESYTVTYEKVQDLRYGENPHQQAAFYKEPIQSRPTLATAKQL
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHH
HGKELSYNNIQDTNAAIEIVKEFAEPAAVAVKHMNPCGIGIGESISTAFERAYQADPTSI
CCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHH
FGGIVACNRPVDAATAEQLSQIFLEIVVAPSFESQALEILTQKKNIRLLELDVTSDNKQS
HCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCC
NRLTTVDGGALIQAYDAKEVSEADLEVVTNKQPTEQEINDMLFAWKAVKHVKSNAIVLAK
CEEEEECCCCEEEECCCCCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
DSQTIGVGAGQMNRIGAAEIAIKQAGDKSEGAVLASDAFFPMPDTVEAAAKAGIKAIIQP
CCCEEECCCCCCCCCCHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHHCCCCEEECC
GGSKRDQDSVDVCNQFGIAMVYTKVRHFKH
CCCCCCCHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SKRALISVSDKTNIIEFAKGLKESGFEILSTGGTLRSIAEAGIDVTPVDEVTGFPEMLD
CCCEEEEECCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCHHHHCCCHHHHC
GRVKTLHPMIHGGLLGKRSNHEHLSQMEEHGIRSIDLVAVNLYPFKETVQKPDVSHQDII
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCHHHHCCCCCCHHHHH
ENIDIGGPSMLRSAAKNFEDVLVVTGPTDYNRVLAAITSETDTYEFRQQLAAKVFRHTAS
HHCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YDAMIANYFLSQTEEQYPESYTVTYEKVQDLRYGENPHQQAAFYKEPIQSRPTLATAKQL
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHH
HGKELSYNNIQDTNAAIEIVKEFAEPAAVAVKHMNPCGIGIGESISTAFERAYQADPTSI
CCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHH
FGGIVACNRPVDAATAEQLSQIFLEIVVAPSFESQALEILTQKKNIRLLELDVTSDNKQS
HCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCC
NRLTTVDGGALIQAYDAKEVSEADLEVVTNKQPTEQEINDMLFAWKAVKHVKSNAIVLAK
CEEEEECCCCEEEECCCCCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
DSQTIGVGAGQMNRIGAAEIAIKQAGDKSEGAVLASDAFFPMPDTVEAAAKAGIKAIIQP
CCCEEECCCCCCCCCCHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHHCCCCEEECC
GGSKRDQDSVDVCNQFGIAMVYTKVRHFKH
CCCCCCCHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376