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The map label for this gene is purN [H]

Identifier: 23098203

GI number: 23098203

Start: 796247

End: 796816

Strand: Direct

Name: purN [H]

Synonym: OB0748

Alternate gene names: 23098203

Gene position: 796247-796816 (Clockwise)

Preceding gene: 23098202

Following gene: 23098204

Centisome position: 21.93

GC content: 36.84

Gene sequence:

>570_bases
ATGACTATAAAAGCAGCTGTTTTTGCTTCAGGTGCGGGTAGTAATTTTGAGGCAATAATGGAAGCAAACGATTTGAAGTG
TAAGATTTCTCTTCTCGTATGCGATAAACCAGGAGCATTAGTGATAGATAAAGCAGCGCGTTATGGTATTCCCACATTGG
TCTTTAATCCAAAGGAATACGGTTCAAAGTCTGAATACGAAGAAATGATTCACCGTCATTTACAACATTATGGAATTTCA
TGGATTTTTTTAGCAGGGTATATGCGACTGATTGGCGATACGCTGCTGAATGAATATGAAAGTAAAATCTTAAATATACA
CCCTTCTTTACTGCCATTTTTTCCTGGAAAAGATGCTATTGGTCAAGCATATGATGCGGGAGCAAGAGAAACAGGGGTTA
GCATTCATTATGTTGATGCAGGGATGGATACGGGACCTGTTATTGCTCAGGAAAGCGTCATGATCGAAGAAAATGATACA
AAAGAAAAATTAAAAGAACGTATACAGAAGGTAGAACATCAACTTTATCCAACAGTAATTAATCAAGTATTGTCTAATAA
AGTGAGGTAA

Upstream 100 bases:

>100_bases
AAGAAGATGTTTCTATCGCATTACAGTTACTCGAAAAAGTAGATGAACAAGCATATGTGATTGGTAAGGTAACAGAGGAG
GAAGGAGTGCACTTTACATT

Downstream 100 bases:

>100_bases
TAACAATGAGTAAACGTGCGTTAATTAGTGTGTCAGATAAAACAAATATAATCGAATTTGCCAAAGGTCTCAAAGAAAGT
GGATTCGAAATCTTATCTAC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MTIKAAVFASGAGSNFEAIMEANDLKCKISLLVCDKPGALVIDKAARYGIPTLVFNPKEYGSKSEYEEMIHRHLQHYGIS
WIFLAGYMRLIGDTLLNEYESKILNIHPSLLPFFPGKDAIGQAYDAGARETGVSIHYVDAGMDTGPVIAQESVMIEENDT
KEKLKERIQKVEHQLYPTVINQVLSNKVR

Sequences:

>Translated_189_residues
MTIKAAVFASGAGSNFEAIMEANDLKCKISLLVCDKPGALVIDKAARYGIPTLVFNPKEYGSKSEYEEMIHRHLQHYGIS
WIFLAGYMRLIGDTLLNEYESKILNIHPSLLPFFPGKDAIGQAYDAGARETGVSIHYVDAGMDTGPVIAQESVMIEENDT
KEKLKERIQKVEHQLYPTVINQVLSNKVR
>Mature_188_residues
TIKAAVFASGAGSNFEAIMEANDLKCKISLLVCDKPGALVIDKAARYGIPTLVFNPKEYGSKSEYEEMIHRHLQHYGISW
IFLAGYMRLIGDTLLNEYESKILNIHPSLLPFFPGKDAIGQAYDAGARETGVSIHYVDAGMDTGPVIAQESVMIEENDTK
EKLKERIQKVEHQLYPTVINQVLSNKVR

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=189, Percent_Identity=35.978835978836, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI209869995, Length=189, Percent_Identity=35.978835978836, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI209869993, Length=189, Percent_Identity=35.978835978836, Blast_Score=139, Evalue=2e-33,
Organism=Escherichia coli, GI1788846, Length=187, Percent_Identity=34.2245989304813, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1787483, Length=190, Percent_Identity=26.8421052631579, Blast_Score=78, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=34.8066298342541, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=35.1063829787234, Blast_Score=141, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21060; Mature: 20929

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKAAVFASGAGSNFEAIMEANDLKCKISLLVCDKPGALVIDKAARYGIPTLVFNPKEY
CCEEEEEEECCCCCCHHEEECCCCCEEEEEEEEECCCCCEEEECHHHCCCCEEEECCHHH
GSKSEYEEMIHRHLQHYGISWIFLAGYMRLIGDTLLNEYESKILNIHPSLLPFFPGKDAI
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
GQAYDAGARETGVSIHYVDAGMDTGPVIAQESVMIEENDTKEKLKERIQKVEHQLYPTVI
HHHHCCCCCCCCCEEEEEECCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
NQVLSNKVR
HHHHHHCCC
>Mature Secondary Structure 
TIKAAVFASGAGSNFEAIMEANDLKCKISLLVCDKPGALVIDKAARYGIPTLVFNPKEY
CEEEEEEECCCCCCHHEEECCCCCEEEEEEEEECCCCCEEEECHHHCCCCEEEECCHHH
GSKSEYEEMIHRHLQHYGISWIFLAGYMRLIGDTLLNEYESKILNIHPSLLPFFPGKDAI
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
GQAYDAGARETGVSIHYVDAGMDTGPVIAQESVMIEENDTKEKLKERIQKVEHQLYPTVI
HHHHCCCCCCCCCEEEEEECCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
NQVLSNKVR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]