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The map label for this gene is purD [H]

Identifier: 23098205

GI number: 23098205

Start: 798372

End: 799643

Strand: Direct

Name: purD [H]

Synonym: OB0750

Alternate gene names: 23098205

Gene position: 798372-799643 (Clockwise)

Preceding gene: 23098204

Following gene: 23098206

Centisome position: 21.99

GC content: 39.31

Gene sequence:

>1272_bases
TTGAACGTTTTAGTAGTCGGCCGAGGCGGCAGAGAACATACCATTGTAAAAAAACTGAAAGCAAATACAAAAATTGTAAA
TCTTTATGTAGCACCAGGAAATGGCGGAATCGCAAATGACGCTACATGTGTGGAAATTGATGAGATGGATATCGATAAAT
TATGTGCATTTGCAAAACAAAACGAAGTGGATTTTACCATCGTCGGCCCAGAAAATCCTCTAAACGCTGGCATTGCAAAT
AAATTTCATGAAGAAGGATTAGCGATTTTTGCTCCAACTAAAGAAGCAGCATTATTGGAAGGAAGTAAGAGCTTTGCAAA
AGACTTTATGAAAAAGTATGCTATTCCAACTGCTGCATATGGCGTATTTATCGATGTGAACGAAGCGAAGAAAATGGTAG
AACAACAAGGTGCTCCTATTGTTATAAAAGCAGATGGCTTAGCAGCTGGAAAAGGTGTAATTGTGGCTATGACAGAAGAA
GAAGCCTACCAAGCAATTGATGATATGTTAATAGATAAAGCATTTTCTAATGCTGGTGCAACTATCGTGATTGAAGAATA
CCTCGAAGGTAAAGAATTTTCTTTAATGGCTTTTGTTCATGAAAATCATGTGTATCCTATGCAGGCTGCAAGAGATCATA
AACGCGCCTTTGATAATGATCAAGGTCCAAATACAGGTGGTATGGGTGCTTTTTCTCCGGTTCCCGATCTAACAGAAGAA
GCATATGACTTTTCTCTTCAGCATATTTTACAAAAAGCTGCAGATGGAATGGCGGAAGAAGGGCGCCCCTTCACAGGTAT
CTTATACGCCGGGTTAATGATGACAGAAGCAGGCCCAAAAGTCATTGAATTTAATACCCGTTTCGGGGATCCGGAAACAC
AAGTTGTCCTACCACTGCTTAACAATGATTTATTGCAAGTAATGCAGGATGTGCTGGAGAAGAAAGACCCACGATTAACC
TGGTATGACGAAGCTTGTGTTGGTGTGGTGCTAGCATCGGCAGGCTATCCGAATGCCTATGAAAAAGGACATATAGTACC
AGAATTTCAATTGCAAGATAAGGATTTTGTCATTCATGCAGGTACAAAAGTGAAAGATGAGAAATTAGTGTCTGATGGGG
GACGCGTTCTTTTAGTAGGCAGTCGTGCGAACACAATGGAAGAAGCAACAGCAAATGTATATCGTCAACTGGATAATACA
ACGATTAATATAGATTCTTTCTTTTACCGAAAAGATATAGCAACAAAAAATGATCATAAAGCTAGAACTTAA

Upstream 100 bases:

>100_bases
AGCGAGACCAAGATTCGGTTGATGTATGTAACCAATTTGGGATTGCTATGGTTTATACAAAGGTAAGACACTTTAAGCAC
TAATAGTAGGAGGGACGCTC

Downstream 100 bases:

>100_bases
GAAATTTATATAAAATGTGAGAGGGTGGGCTTTGCAAATTAAATGGAGGCCCACCCTTTCTATGTCTGAACCGCCAATAG
TATTTGGAAAATTTTAGATC

Product: phosphoribosylglycinamide synthetase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MNVLVVGRGGREHTIVKKLKANTKIVNLYVAPGNGGIANDATCVEIDEMDIDKLCAFAKQNEVDFTIVGPENPLNAGIAN
KFHEEGLAIFAPTKEAALLEGSKSFAKDFMKKYAIPTAAYGVFIDVNEAKKMVEQQGAPIVIKADGLAAGKGVIVAMTEE
EAYQAIDDMLIDKAFSNAGATIVIEEYLEGKEFSLMAFVHENHVYPMQAARDHKRAFDNDQGPNTGGMGAFSPVPDLTEE
AYDFSLQHILQKAADGMAEEGRPFTGILYAGLMMTEAGPKVIEFNTRFGDPETQVVLPLLNNDLLQVMQDVLEKKDPRLT
WYDEACVGVVLASAGYPNAYEKGHIVPEFQLQDKDFVIHAGTKVKDEKLVSDGGRVLLVGSRANTMEEATANVYRQLDNT
TINIDSFFYRKDIATKNDHKART

Sequences:

>Translated_423_residues
MNVLVVGRGGREHTIVKKLKANTKIVNLYVAPGNGGIANDATCVEIDEMDIDKLCAFAKQNEVDFTIVGPENPLNAGIAN
KFHEEGLAIFAPTKEAALLEGSKSFAKDFMKKYAIPTAAYGVFIDVNEAKKMVEQQGAPIVIKADGLAAGKGVIVAMTEE
EAYQAIDDMLIDKAFSNAGATIVIEEYLEGKEFSLMAFVHENHVYPMQAARDHKRAFDNDQGPNTGGMGAFSPVPDLTEE
AYDFSLQHILQKAADGMAEEGRPFTGILYAGLMMTEAGPKVIEFNTRFGDPETQVVLPLLNNDLLQVMQDVLEKKDPRLT
WYDEACVGVVLASAGYPNAYEKGHIVPEFQLQDKDFVIHAGTKVKDEKLVSDGGRVLLVGSRANTMEEATANVYRQLDNT
TINIDSFFYRKDIATKNDHKART
>Mature_423_residues
MNVLVVGRGGREHTIVKKLKANTKIVNLYVAPGNGGIANDATCVEIDEMDIDKLCAFAKQNEVDFTIVGPENPLNAGIAN
KFHEEGLAIFAPTKEAALLEGSKSFAKDFMKKYAIPTAAYGVFIDVNEAKKMVEQQGAPIVIKADGLAAGKGVIVAMTEE
EAYQAIDDMLIDKAFSNAGATIVIEEYLEGKEFSLMAFVHENHVYPMQAARDHKRAFDNDQGPNTGGMGAFSPVPDLTEE
AYDFSLQHILQKAADGMAEEGRPFTGILYAGLMMTEAGPKVIEFNTRFGDPETQVVLPLLNNDLLQVMQDVLEKKDPRLT
WYDEACVGVVLASAGYPNAYEKGHIVPEFQLQDKDFVIHAGTKVKDEKLVSDGGRVLLVGSRANTMEEATANVYRQLDNT
TINIDSFFYRKDIATKNDHKART

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=44.4705882352941, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=44.4705882352941, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=44.4705882352941, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=44.4705882352941, Blast_Score=340, Evalue=2e-93,
Organism=Escherichia coli, GI1790438, Length=424, Percent_Identity=47.6415094339623, Blast_Score=368, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17567511, Length=428, Percent_Identity=39.018691588785, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6321203, Length=439, Percent_Identity=39.6355353075171, Blast_Score=289, Evalue=5e-79,
Organism=Drosophila melanogaster, GI62471619, Length=427, Percent_Identity=41.2177985948478, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24582400, Length=427, Percent_Identity=41.2177985948478, Blast_Score=313, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46321; Mature: 46321

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVVGRGGREHTIVKKLKANTKIVNLYVAPGNGGIANDATCVEIDEMDIDKLCAFAKQ
CCEEEEECCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHCC
NEVDFTIVGPENPLNAGIANKFHEEGLAIFAPTKEAALLEGSKSFAKDFMKKYAIPTAAY
CCCEEEEECCCCCCCCCHHHHHHHCCEEEEECCCCHHHHCCCHHHHHHHHHHHCCCCCCE
GVFIDVNEAKKMVEQQGAPIVIKADGLAAGKGVIVAMTEEEAYQAIDDMLIDKAFSNAGA
EEEEECHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCC
TIVIEEYLEGKEFSLMAFVHENHVYPMQAARDHKRAFDNDQGPNTGGMGAFSPVPDLTEE
EEEEHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
AYDFSLQHILQKAADGMAEEGRPFTGILYAGLMMTEAGPKVIEFNTRFGDPETQVVLPLL
HHHHHHHHHHHHHHCCHHHCCCCEEHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEHH
NNDLLQVMQDVLEKKDPRLTWYDEACVGVVLASAGYPNAYEKGHIVPEFQLQDKDFVIHA
CCHHHHHHHHHHHCCCCCEEEEHHHHEEHHEECCCCCCCCCCCCCCCCEEECCCCEEEEC
GTKVKDEKLVSDGGRVLLVGSRANTMEEATANVYRQLDNTTINIDSFFYRKDIATKNDHK
CCCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCCCCC
ART
CCC
>Mature Secondary Structure
MNVLVVGRGGREHTIVKKLKANTKIVNLYVAPGNGGIANDATCVEIDEMDIDKLCAFAKQ
CCEEEEECCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHCC
NEVDFTIVGPENPLNAGIANKFHEEGLAIFAPTKEAALLEGSKSFAKDFMKKYAIPTAAY
CCCEEEEECCCCCCCCCHHHHHHHCCEEEEECCCCHHHHCCCHHHHHHHHHHHCCCCCCE
GVFIDVNEAKKMVEQQGAPIVIKADGLAAGKGVIVAMTEEEAYQAIDDMLIDKAFSNAGA
EEEEECHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCC
TIVIEEYLEGKEFSLMAFVHENHVYPMQAARDHKRAFDNDQGPNTGGMGAFSPVPDLTEE
EEEEHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
AYDFSLQHILQKAADGMAEEGRPFTGILYAGLMMTEAGPKVIEFNTRFGDPETQVVLPLL
HHHHHHHHHHHHHHCCHHHCCCCEEHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEHH
NNDLLQVMQDVLEKKDPRLTWYDEACVGVVLASAGYPNAYEKGHIVPEFQLQDKDFVIHA
CCHHHHHHHHHHHCCCCCEEEEHHHHEEHHEECCCCCCCCCCCCCCCCEEECCCCEEEEC
GTKVKDEKLVSDGGRVLLVGSRANTMEEATANVYRQLDNTTINIDSFFYRKDIATKNDHK
CCCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCCCCC
ART
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]