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The map label for this gene is purB [H]

Identifier: 23098196

GI number: 23098196

Start: 788655

End: 789950

Strand: Direct

Name: purB [H]

Synonym: OB0741

Alternate gene names: 23098196

Gene position: 788655-789950 (Clockwise)

Preceding gene: 23098195

Following gene: 23098197

Centisome position: 21.72

GC content: 38.04

Gene sequence:

>1296_bases
ATGATTGAACGTTATACAAGAGAAGAAATGGGTAGTATTTGGACGGAAGAGAATAAATTCAAAGCGTGGTTAGAGGTTGA
AATACTAGCTTGCGAAGCATGGAGCGAGTTAGGTGTTATCCCGAAAGAAGATGTAAAGAAATTAAGAGAAAATGCTTCTT
TTGATATGAACCGCATTTATGAAATTGAAGAAGAAACACGACATGATGTAGTGGCATTTACACGTGCTGTATCCGAGACA
TTAGGGGAAGAGCGTAAATGGGTACATTATGGTTTAACATCGACTGACGTTGTAGATACAGCATTATCTTATATTTTAAA
ACAAGCAAACGATATTCTACGTAAAGACTTAAATCAATTTGTTGAAATTCTGAAAGAAAAAGCAATAGAGCATAAACATA
CGGTAATGATGGGACGCACACATGGTGTTCATGCAGAACCAACAACATTCGGATTGAAAATGGCATTATGGTATGAAGAA
ATGAAACGAAATGTAGAGCGTTTTGAACTGGCTGCGAAGAACATCGAATTTGGTAAGATTTCTGGTGCAGTGGGTACTTA
TGCAAATATCGATCCATTTGTGGAACAGTATGTGTGTGAAAAATTAGGTACTTCTCCAGCTCCTGTGTCCACACAAACAT
TACAACGTGATCGTCATGCTGCATATTTATCGGCTCTTGCATTAATTGCAACGTCGATCGAAAAGTTCGCAACAGAAATT
CGTGGATTGCAGAAAACAGAAACACGTGAAGTAGAAGAGCGATTTGCGAAAGGTCAGAAAGGCTCGTCTGCAATGCCGCA
TAAACGTAATCCAATCGGATCAGAAAATATGACTGGTATGGCTCGTGTGATTCGAGGATATATGGTAACAGCCTATGAAA
ATGTGGCTTTATGGCATGAACGTGATATTTCTCATTCATCAGCAGAGAGAGTAATCTTACCAGACGCGACTATTGCTTTA
AATTATATGTTAAATCGTTTTGGTAATATCGTAAAAAACTTAACGGTGTTCCCAGAGAATATGAAGCGTAATATTGATCG
CACACATGGGGTTATTTTCTCCCAAAGAGTGTTGCTTTCATTAATTGATAAAGGCATGGCTCGTGAGGAAGCATATGACA
TTGTACAACCAAAAGCAATGGATGCTTGGGAAACAGAGACACATTTCAAACAGTTGGTAGAAAGTGATGACCGAATTACT
GGAAAGCTAACGCAAGCAGAGATTGATGATTGTTTTGATTATACGTATCACTTGAAGAATGTCGATCAAATCTTTACAAA
AATCGGTATTTCCTAG

Upstream 100 bases:

>100_bases
AGCCGAAAGAAAAACGAAAAATGGGACATATTACATTCACTGCGAAGACAACAGAAGAACTAGAGAAACAAGTGAATTAC
TTCAAGGAGGAAATACATTC

Downstream 100 bases:

>100_bases
GAGGCTCATATAGTTATGAAAGCAGCACTTTTATATGAAGGTAAAGCGAAGAAAGTATATCAATCTTCTGAAGATGAACA
TCAATTAGTGCTCTCCTATA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTREEMGSIWTEENKFKAWLEVEILACEAWSELGVIPKEDVKKLRENASFDMNRIYEIEEETRHDVVAFTRAVSET
LGEERKWVHYGLTSTDVVDTALSYILKQANDILRKDLNQFVEILKEKAIEHKHTVMMGRTHGVHAEPTTFGLKMALWYEE
MKRNVERFELAAKNIEFGKISGAVGTYANIDPFVEQYVCEKLGTSPAPVSTQTLQRDRHAAYLSALALIATSIEKFATEI
RGLQKTETREVEERFAKGQKGSSAMPHKRNPIGSENMTGMARVIRGYMVTAYENVALWHERDISHSSAERVILPDATIAL
NYMLNRFGNIVKNLTVFPENMKRNIDRTHGVIFSQRVLLSLIDKGMAREEAYDIVQPKAMDAWETETHFKQLVESDDRIT
GKLTQAEIDDCFDYTYHLKNVDQIFTKIGIS

Sequences:

>Translated_431_residues
MIERYTREEMGSIWTEENKFKAWLEVEILACEAWSELGVIPKEDVKKLRENASFDMNRIYEIEEETRHDVVAFTRAVSET
LGEERKWVHYGLTSTDVVDTALSYILKQANDILRKDLNQFVEILKEKAIEHKHTVMMGRTHGVHAEPTTFGLKMALWYEE
MKRNVERFELAAKNIEFGKISGAVGTYANIDPFVEQYVCEKLGTSPAPVSTQTLQRDRHAAYLSALALIATSIEKFATEI
RGLQKTETREVEERFAKGQKGSSAMPHKRNPIGSENMTGMARVIRGYMVTAYENVALWHERDISHSSAERVILPDATIAL
NYMLNRFGNIVKNLTVFPENMKRNIDRTHGVIFSQRVLLSLIDKGMAREEAYDIVQPKAMDAWETETHFKQLVESDDRIT
GKLTQAEIDDCFDYTYHLKNVDQIFTKIGIS
>Mature_431_residues
MIERYTREEMGSIWTEENKFKAWLEVEILACEAWSELGVIPKEDVKKLRENASFDMNRIYEIEEETRHDVVAFTRAVSET
LGEERKWVHYGLTSTDVVDTALSYILKQANDILRKDLNQFVEILKEKAIEHKHTVMMGRTHGVHAEPTTFGLKMALWYEE
MKRNVERFELAAKNIEFGKISGAVGTYANIDPFVEQYVCEKLGTSPAPVSTQTLQRDRHAAYLSALALIATSIEKFATEI
RGLQKTETREVEERFAKGQKGSSAMPHKRNPIGSENMTGMARVIRGYMVTAYENVALWHERDISHSSAERVILPDATIAL
NYMLNRFGNIVKNLTVFPENMKRNIDRTHGVIFSQRVLLSLIDKGMAREEAYDIVQPKAMDAWETETHFKQLVESDDRIT
GKLTQAEIDDCFDYTYHLKNVDQIFTKIGIS

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=398, Percent_Identity=29.1457286432161, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4557269, Length=390, Percent_Identity=28.7179487179487, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1787376, Length=318, Percent_Identity=27.3584905660377, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17508577, Length=398, Percent_Identity=26.8844221105528, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32564234, Length=341, Percent_Identity=27.8592375366569, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323391, Length=387, Percent_Identity=28.9405684754522, Blast_Score=163, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24647570, Length=393, Percent_Identity=26.4631043256997, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49471; Mature: 49471

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTREEMGSIWTEENKFKAWLEVEILACEAWSELGVIPKEDVKKLRENASFDMNRIY
CCCCHHHHHHHHHCCCCCCEEEEEEEEEEEEHHHHHCCCCCHHHHHHHHHCCCCCHHHHH
EIEEETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTALSYILKQANDILRKDLNQF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEILKEKAIEHKHTVMMGRTHGVHAEPTTFGLKMALWYEEMKRNVERFELAAKNIEFGKI
HHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SGAVGTYANIDPFVEQYVCEKLGTSPAPVSTQTLQRDRHAAYLSALALIATSIEKFATEI
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLQKTETREVEERFAKGQKGSSAMPHKRNPIGSENMTGMARVIRGYMVTAYENVALWHE
HCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSAERVILPDATIALNYMLNRFGNIVKNLTVFPENMKRNIDRTHGVIFSQRVLLS
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LIDKGMAREEAYDIVQPKAMDAWETETHFKQLVESDDRITGKLTQAEIDDCFDYTYHLKN
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
VDQIFTKIGIS
HHHHHHHHCCC
>Mature Secondary Structure
MIERYTREEMGSIWTEENKFKAWLEVEILACEAWSELGVIPKEDVKKLRENASFDMNRIY
CCCCHHHHHHHHHCCCCCCEEEEEEEEEEEEHHHHHCCCCCHHHHHHHHHCCCCCHHHHH
EIEEETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTALSYILKQANDILRKDLNQF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEILKEKAIEHKHTVMMGRTHGVHAEPTTFGLKMALWYEEMKRNVERFELAAKNIEFGKI
HHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SGAVGTYANIDPFVEQYVCEKLGTSPAPVSTQTLQRDRHAAYLSALALIATSIEKFATEI
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLQKTETREVEERFAKGQKGSSAMPHKRNPIGSENMTGMARVIRGYMVTAYENVALWHE
HCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSAERVILPDATIALNYMLNRFGNIVKNLTVFPENMKRNIDRTHGVIFSQRVLLS
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LIDKGMAREEAYDIVQPKAMDAWETETHFKQLVESDDRITGKLTQAEIDDCFDYTYHLKN
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
VDQIFTKIGIS
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]