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The map label for this gene is ksgA [H]

Identifier: 22127557

GI number: 22127557

Start: 4094820

End: 4095638

Strand: Direct

Name: ksgA [H]

Synonym: y3683

Alternate gene names: 22127557

Gene position: 4094820-4095638 (Clockwise)

Preceding gene: 22127556

Following gene: 22127558

Centisome position: 89.0

GC content: 47.62

Gene sequence:

>819_bases
ATGAATAATAGAGTCCACCAAGGGCATTTTGCCCGCAAACGCTTTGGACAAAACTTTTTAAACGATCAGTTTGTCATCGA
CAGTATTGTCTCCGCAATCCACCCAGTTCCTGGCGAAGCGGTCGTTGAAATTGGCCCCGGTTTAGGTGCCTTAACCGAAC
CCGTCGCGGCTCGTATGGATCACATGACCGTTATTGAGCTGGATCGCGATCTGGCTGCACGGCTGGCTAGCCATCCTCAA
CTGAAAGATAAGCTAACCATTCATCAGCAAGATGCGATGAAAGTAAACTTTTCCGAACTGTCAGAGCAAGCGGGGCAACC
ATTACGGGTATTTGGTAACCTGCCATACAATATTTCTACGCCGCTGATGTTCCATCTTTTCAGCTATACTGATGCTATCC
GCGACATGCATTTCATGTTGCAAAAAGAGGTTGTTAACCGCTTGGTTGCTGGCCCCAACAGTAAAACTTATGGCCGTCTG
ACCGTCATGGCGCAATACTATTGCAATGTGATCCCGGTTTTGGAAGTACCGCCGACTGCGTTTACCCCAGCCCCAAAAGT
TGATTCCGCAGTTGTGCGCCTCATTCCACATGTGCAAATGCCTCACCCGGTCGGTGACGTTCGTATGCTGAGCCGTATTA
CTACGCAAGCCTTTAACCAACGTAGAAAAACGGTGCGCAATAGCTTAGGTGATTTATTCACTTCAGAGCAATTAATCGAA
TTAGGTATTGACCCGATACTCAGGGCAGAAAATATTTCGGTAGCGCAATACTGTAAGCTAGCTAACTGGCTTTCAGCTCA
ATCGACACCGCAGAAATAA

Upstream 100 bases:

>100_bases
GATCACGGTACCGCTTTAGAACTCGCCGCAACCGGTACTGCCGATGTTGGCAGTTTCATTACGGCATTAAACTTAGCCAT
CAAAATGATAAATAACAGCA

Downstream 100 bases:

>100_bases
GCAGGAGCACATCATGATTGAACAGCCCCGTATTTGTGTGCAGGTACACAGTATCTATGTGGAAACGCAATCGATACCCG
AAGAGGAACGTTTCGTCTTC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MNNRVHQGHFARKRFGQNFLNDQFVIDSIVSAIHPVPGEAVVEIGPGLGALTEPVAARMDHMTVIELDRDLAARLASHPQ
LKDKLTIHQQDAMKVNFSELSEQAGQPLRVFGNLPYNISTPLMFHLFSYTDAIRDMHFMLQKEVVNRLVAGPNSKTYGRL
TVMAQYYCNVIPVLEVPPTAFTPAPKVDSAVVRLIPHVQMPHPVGDVRMLSRITTQAFNQRRKTVRNSLGDLFTSEQLIE
LGIDPILRAENISVAQYCKLANWLSAQSTPQK

Sequences:

>Translated_272_residues
MNNRVHQGHFARKRFGQNFLNDQFVIDSIVSAIHPVPGEAVVEIGPGLGALTEPVAARMDHMTVIELDRDLAARLASHPQ
LKDKLTIHQQDAMKVNFSELSEQAGQPLRVFGNLPYNISTPLMFHLFSYTDAIRDMHFMLQKEVVNRLVAGPNSKTYGRL
TVMAQYYCNVIPVLEVPPTAFTPAPKVDSAVVRLIPHVQMPHPVGDVRMLSRITTQAFNQRRKTVRNSLGDLFTSEQLIE
LGIDPILRAENISVAQYCKLANWLSAQSTPQK
>Mature_272_residues
MNNRVHQGHFARKRFGQNFLNDQFVIDSIVSAIHPVPGEAVVEIGPGLGALTEPVAARMDHMTVIELDRDLAARLASHPQ
LKDKLTIHQQDAMKVNFSELSEQAGQPLRVFGNLPYNISTPLMFHLFSYTDAIRDMHFMLQKEVVNRLVAGPNSKTYGRL
TVMAQYYCNVIPVLEVPPTAFTPAPKVDSAVVRLIPHVQMPHPVGDVRMLSRITTQAFNQRRKTVRNSLGDLFTSEQLIE
LGIDPILRAENISVAQYCKLANWLSAQSTPQK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=279, Percent_Identity=31.8996415770609, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI7657198, Length=248, Percent_Identity=28.6290322580645, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1786236, Length=272, Percent_Identity=79.7794117647059, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI25141369, Length=292, Percent_Identity=27.0547945205479, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25146882, Length=186, Percent_Identity=34.4086021505376, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324989, Length=214, Percent_Identity=29.9065420560748, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357273, Length=278, Percent_Identity=29.136690647482, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21358017, Length=229, Percent_Identity=33.1877729257642, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30459; Mature: 30459

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRVHQGHFARKRFGQNFLNDQFVIDSIVSAIHPVPGEAVVEIGPGLGALTEPVAARMD
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHC
HMTVIELDRDLAARLASHPQLKDKLTIHQQDAMKVNFSELSEQAGQPLRVFGNLPYNIST
CCEEEEHHHHHHHHHHCCCCHHHHHEEECCCCCEECHHHHHHHCCCCEEEECCCCCCCCC
PLMFHLFSYTDAIRDMHFMLQKEVVNRLVAGPNSKTYGRLTVMAQYYCNVIPVLEVPPTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCEEEEEECCCCC
FTPAPKVDSAVVRLIPHVQMPHPVGDVRMLSRITTQAFNQRRKTVRNSLGDLFTSEQLIE
CCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGIDPILRAENISVAQYCKLANWLSAQSTPQK
HCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNNRVHQGHFARKRFGQNFLNDQFVIDSIVSAIHPVPGEAVVEIGPGLGALTEPVAARMD
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHC
HMTVIELDRDLAARLASHPQLKDKLTIHQQDAMKVNFSELSEQAGQPLRVFGNLPYNIST
CCEEEEHHHHHHHHHHCCCCHHHHHEEECCCCCEECHHHHHHHCCCCEEEECCCCCCCCC
PLMFHLFSYTDAIRDMHFMLQKEVVNRLVAGPNSKTYGRLTVMAQYYCNVIPVLEVPPTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCEEEEEECCCCC
FTPAPKVDSAVVRLIPHVQMPHPVGDVRMLSRITTQAFNQRRKTVRNSLGDLFTSEQLIE
CCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGIDPILRAENISVAQYCKLANWLSAQSTPQK
HCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA