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The map label for this gene is leuB

Identifier: 161484746

GI number: 161484746

Start: 4043789

End: 4044880

Strand: Direct

Name: leuB

Synonym: y3646

Alternate gene names: 161484746

Gene position: 4043789-4044880 (Clockwise)

Preceding gene: 22127519

Following gene: 22127521

Centisome position: 87.89

GC content: 50.92

Gene sequence:

>1092_bases
ATGACGAAGACTTATCATATTGCAGTGTTGCCCGGCGACGGTATCGGCCCTGAAGTAATGGCTCAGGCAAGTAAAGTATT
AGATGCGGTGCGTCAGCGCTTCGGTCTCAAGATTTCAACCAGTGTTTACGATGTTGGTGGCGCTGCCATTGACCGCCATG
GTAGCCCATTACCGGCAGCAACGGTCGCGGGATGTGAACAGGCAGATGCCATTTTATTTGGCTCCGTAGGCGGCCCAAAA
TGGGAACACCTGCCACCGGCAGAACAACCTGAGCGTGGTGCCTTATTGCCTTTGCGTAAACATTTCAAATTATTCAGCAA
TTTGCGCCCTGCCCGTTTGTATCAAGGATTGGAGGACTTCTGTCCATTGCGCAGTGATATCGCGGCCAGAGGTTTTGATA
TCCTGTGTGTCCGTGAATTAACCGGCGGTATCTATTTCGGCCAGCCGAAAGGCCGTGAAGGCCAAGGCATGCACGAGCGG
GCCTTTGACACCGAAGTTTATCATCGCTTCGAAATTGAACGTATCGCCCGTATTGCTTTCGAATCAGCCCGCAAACGCCG
TAGTAAAGTAACGTCCATTGATAAAGCCAACGTGTTGCAAAGTTCAATCTTGTGGCGTGAAGTGGTCAACGGGATCGCGG
CGGATTACCCAGATGTTGCGTTGTCCCATATGTACATTGATAACGCCACCATGCAGTTAATCAAAGACCCTTCCCAATTT
GACGTGTTGCTGTGCTCTAACTTGTTTGGCGATATCTTGTCCGATGAATGCGCCATGATCACCGGCTCCATGGGCATGTT
GCCATCAGCCAGTTTGAATGAACAAGGTTTTGGTCTATATGAACCCGCAGGCGGTTCCGCACCGGATATCGCGGGTAAGA
ACATTGCCAACCCTATCGCTCAGATCCTGTCATTAACCCTGTTGCTGCGTTTTAGCTTAGGTAAAGATGATGCCGCTGAC
GCCATTGAGCGCGCGATCAACCAGGCATTGGAGCAAGGTTATCGCACTGCGGATTTAGCCGGGGATGGTCACGCAATCGG
CACTCACGAAATGGGCGATATCATCGCTAAATTCGTGGTTGAAGGGGTATAA

Upstream 100 bases:

>100_bases
CTAAGGCGTTGGTTCACGTATTAAATAACATCTGGCGCGCCCATCAGGTCGAAAAAGAGAAGCAGCGTCTGCAACAAAAT
AATCAGGAAATGGTGTGAAC

Downstream 100 bases:

>100_bases
AATGGGCACAACATCATCTCAGAGCAAAACGTTATACCAAAAATTGTACGATGCGCACATTGTGCATGAAGCCCCCAATG
AGACCCCATTGCTGTATATC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTKTYHIAVLPGDGIGPEVMAQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAATVAGCEQADAILFGSVGGPK
WEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDFCPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHER
AFDTEVYHRFEIERIARIAFESARKRRSKVTSIDKANVLQSSILWREVVNGIAADYPDVALSHMYIDNATMQLIKDPSQF
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILSLTLLLRFSLGKDDAAD
AIERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVVEGV

Sequences:

>Translated_363_residues
MTKTYHIAVLPGDGIGPEVMAQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAATVAGCEQADAILFGSVGGPK
WEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDFCPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHER
AFDTEVYHRFEIERIARIAFESARKRRSKVTSIDKANVLQSSILWREVVNGIAADYPDVALSHMYIDNATMQLIKDPSQF
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILSLTLLLRFSLGKDDAAD
AIERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVVEGV
>Mature_362_residues
TKTYHIAVLPGDGIGPEVMAQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAATVAGCEQADAILFGSVGGPKW
EHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDFCPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHERA
FDTEVYHRFEIERIARIAFESARKRRSKVTSIDKANVLQSSILWREVVNGIAADYPDVALSHMYIDNATMQLIKDPSQFD
VLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILSLTLLLRFSLGKDDAADA
IERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVVEGV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=341, Percent_Identity=33.4310850439883, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4758582, Length=357, Percent_Identity=28.2913165266106, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI28178821, Length=315, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI28178816, Length=315, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI28178838, Length=331, Percent_Identity=26.5861027190332, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178819, Length=150, Percent_Identity=36.6666666666667, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=85.9504132231405, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1788101, Length=371, Percent_Identity=38.544474393531, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1787381, Length=398, Percent_Identity=26.1306532663317, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=342, Percent_Identity=32.7485380116959, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17505779, Length=352, Percent_Identity=30.1136363636364, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=343, Percent_Identity=30.3206997084548, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17550882, Length=345, Percent_Identity=28.9855072463768, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=370, Percent_Identity=43.2432432432432, Blast_Score=269, Evalue=6e-73,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=33.8068181818182, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=347, Percent_Identity=30.835734870317, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324291, Length=344, Percent_Identity=29.9418604651163, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=32.8445747800587, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=32.8445747800587, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24661184, Length=344, Percent_Identity=29.3604651162791, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI161078637, Length=339, Percent_Identity=29.4985250737463, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078635, Length=339, Percent_Identity=29.4985250737463, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161078633, Length=339, Percent_Identity=29.2035398230088, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24650122, Length=339, Percent_Identity=29.2035398230088, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161078639, Length=337, Percent_Identity=29.673590504451, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI281362242, Length=340, Percent_Identity=26.7647058823529, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648872, Length=340, Percent_Identity=26.7647058823529, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI20130355, Length=344, Percent_Identity=24.4186046511628, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_YERPE (Q8ZIG9)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AB0066
- ProteinModelPortal:   Q8ZIG9
- SMR:   Q8ZIG9
- IntAct:   Q8ZIG9
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO0532
- KEGG:   ypk:y3646
- KEGG:   ypm:YP_3650
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   YPES187410:Y3646-MONOMER
- BioCyc:   YPES214092:YPO0532-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 39267; Mature: 39136

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 99-99 BINDING 109-109 BINDING 138-138 BINDING 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTYHIAVLPGDGIGPEVMAQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAA
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCEEECCCHHHCCCCCCCCHH
TVAGCEQADAILFGSVGGPKWEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDF
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCHHHH
CPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHERAFDTEVYHRFEIERIARIAF
CCCHHHHHHCCCCEEEEEHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ESARKRRSKVTSIDKANVLQSSILWREVVNGIAADYPDVALSHMYIDNATMQLIKDPSQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCCCHH
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIA
HHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHH
QILSLTLLLRFSLGKDDAADAIERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
EGV
CCC
>Mature Secondary Structure 
TKTYHIAVLPGDGIGPEVMAQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAA
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCEEECCCHHHCCCCCCCCHH
TVAGCEQADAILFGSVGGPKWEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDF
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCHHHH
CPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHERAFDTEVYHRFEIERIARIAF
CCCHHHHHHCCCCEEEEEHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ESARKRRSKVTSIDKANVLQSSILWREVVNGIAADYPDVALSHMYIDNATMQLIKDPSQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCCCHH
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIA
HHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHH
QILSLTLLLRFSLGKDDAADAIERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
EGV
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430