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The map label for this gene is leuA [H]

Identifier: 22127519

GI number: 22127519

Start: 4042224

End: 4043786

Strand: Direct

Name: leuA [H]

Synonym: y3645

Alternate gene names: 22127519

Gene position: 4042224-4043786 (Clockwise)

Preceding gene: 22127518

Following gene: 161484746

Centisome position: 87.86

GC content: 47.79

Gene sequence:

>1563_bases
ATGAGCCAACAAGTCATTATTTTTGATACAACATTGCGCGACGGTGAACAAGCATTGCAAGCCAGTCTGAGTGTGAAAGA
GAAGCTGCAAATCGCGCTGGCATTGGAAAGAATGGGGGTCGATATCATGGAGGTGGGTTTCCCTGTCTCCTCCCCTGGTG
ATTTTGAATCGGTTCGTACTATCGCTCAGCAAGTGAAAAATAGCCGGGTTTGCGCCTTGGCACGTTGTGTGGATAAAGAT
ATTGATGTTGCGGCAGAGGCATTGCGAATTGCAGAAGCTTTCCGTATTCATGTGTTTTTGGCCACCTCAACCTTGCATAT
CGAATCCAAGTTAAAGCGTTCGTTTGACGATGTGCTGGCGATGGCGGTCCACTCCGTGAAACGTGCTCGTAACTATACCG
ATGACGTCGAGTTTTCTTGTGAAGATGCTGGCCGTACTCCAATTGATAACTTATGCCGTGTCGTCGAGGCCGCGATTACC
GCCGGTGCCACCACAATCAATATCCCGGATACCGTCGGTTACACCCCCCCTTATCAATTTGGTGGCATCATCACTGACTT
GTATGAACGTGTTCCCAATATTGATAAAGCCATTATCTCTGTTCATTGCCATGATGACTTGGGCATGTCCGTTGCCAACT
CCATTACTGCAGTACAAGCCGGGGCGCGTCAGGTCGAAGGGACCATCAACGGCTTAGGTGAGCGTGCAGGTAACTGTTCA
TTGGAAGAGGTGATCATGGCCATTAAAGTGCGCCATGAAATGTTGGGCGTGCACACCAATATCAATCATCAGGAAATTTA
CCGTACCAGCCAGTTGGTCAGTAAAATTTGCAATATGCCTATTCCCGGCAATAAAGCCATTGTCGGCAGTAATGCCTTCG
CTCACTCCTCCGGTATTCATCAGGATGGTGTCCTGAAAAATCGCGAAAACTACGAAATCATGACCCCGGAATCCATCGGT
CTGAAAGAAGTCCAATTGAACCTGACATCCCGTTCAGGTCGTGCGGCAGTGAAGCACCGGATGGAAGAGATGGGTTATCA
GGATAAAGATTACAATTTAGATTCTTTGTACGATGCCTTCTTGAAATTAGCCGATAAAAAAGGCCAGGTCTTTGACTACG
ATTTGGAAGCATTGGCCTTTATTAATAAGCAGCAGGAAGAGCCTGAATATTATCGTTTGGACTATTTCAGCGTCCAGTCG
GGTTCCAGTGTCATGGCAACTGCCTCAGTAAAATTGGTCTGTGGTGAAGAAATAAAATCAGAAGCGGCCACCGGTAACGG
CCCGGTTGATGCGGTGTATCAGGCCATCAATCGCATTACCGACTACCCAATTGAACTGGTGAAGTATCAACTGTCGGCCA
AAGGTCAGGGTAAAGATGCATTGGGTCAGGTTGATATCGTGGTTGACCACAAGGGGCGTCGCTTCCATGGCGTAGGTTTG
GCAACTGACATTGTTGAGTCATCCGCTAAGGCGTTGGTTCACGTATTAAATAACATCTGGCGCGCCCATCAGGTCGAAAA
AGAGAAGCAGCGTCTGCAACAAAATAATCAGGAAATGGTGTGA

Upstream 100 bases:

>100_bases
CTGGTAACAGAGCCTTATAGGCGCATATGAAAAACCTCCGGCTATGCCGGAGGATATTTATTTTATCCGCTGAGCGCAAA
TGTAAAAAAAGGAACAACCC

Downstream 100 bases:

>100_bases
ACATGACGAAGACTTATCATATTGCAGTGTTGCCCGGCGACGGTATCGGCCCTGAAGTAATGGCTCAGGCAAGTAAAGTA
TTAGATGCGGTGCGTCAGCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDIMEVGFPVSSPGDFESVRTIAQQVKNSRVCALARCVDKD
IDVAAEALRIAEAFRIHVFLATSTLHIESKLKRSFDDVLAMAVHSVKRARNYTDDVEFSCEDAGRTPIDNLCRVVEAAIT
AGATTINIPDTVGYTPPYQFGGIITDLYERVPNIDKAIISVHCHDDLGMSVANSITAVQAGARQVEGTINGLGERAGNCS
LEEVIMAIKVRHEMLGVHTNINHQEIYRTSQLVSKICNMPIPGNKAIVGSNAFAHSSGIHQDGVLKNRENYEIMTPESIG
LKEVQLNLTSRSGRAAVKHRMEEMGYQDKDYNLDSLYDAFLKLADKKGQVFDYDLEALAFINKQQEEPEYYRLDYFSVQS
GSSVMATASVKLVCGEEIKSEAATGNGPVDAVYQAINRITDYPIELVKYQLSAKGQGKDALGQVDIVVDHKGRRFHGVGL
ATDIVESSAKALVHVLNNIWRAHQVEKEKQRLQQNNQEMV

Sequences:

>Translated_520_residues
MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDIMEVGFPVSSPGDFESVRTIAQQVKNSRVCALARCVDKD
IDVAAEALRIAEAFRIHVFLATSTLHIESKLKRSFDDVLAMAVHSVKRARNYTDDVEFSCEDAGRTPIDNLCRVVEAAIT
AGATTINIPDTVGYTPPYQFGGIITDLYERVPNIDKAIISVHCHDDLGMSVANSITAVQAGARQVEGTINGLGERAGNCS
LEEVIMAIKVRHEMLGVHTNINHQEIYRTSQLVSKICNMPIPGNKAIVGSNAFAHSSGIHQDGVLKNRENYEIMTPESIG
LKEVQLNLTSRSGRAAVKHRMEEMGYQDKDYNLDSLYDAFLKLADKKGQVFDYDLEALAFINKQQEEPEYYRLDYFSVQS
GSSVMATASVKLVCGEEIKSEAATGNGPVDAVYQAINRITDYPIELVKYQLSAKGQGKDALGQVDIVVDHKGRRFHGVGL
ATDIVESSAKALVHVLNNIWRAHQVEKEKQRLQQNNQEMV
>Mature_519_residues
SQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDIMEVGFPVSSPGDFESVRTIAQQVKNSRVCALARCVDKDI
DVAAEALRIAEAFRIHVFLATSTLHIESKLKRSFDDVLAMAVHSVKRARNYTDDVEFSCEDAGRTPIDNLCRVVEAAITA
GATTINIPDTVGYTPPYQFGGIITDLYERVPNIDKAIISVHCHDDLGMSVANSITAVQAGARQVEGTINGLGERAGNCSL
EEVIMAIKVRHEMLGVHTNINHQEIYRTSQLVSKICNMPIPGNKAIVGSNAFAHSSGIHQDGVLKNRENYEIMTPESIGL
KEVQLNLTSRSGRAAVKHRMEEMGYQDKDYNLDSLYDAFLKLADKKGQVFDYDLEALAFINKQQEEPEYYRLDYFSVQSG
SSVMATASVKLVCGEEIKSEAATGNGPVDAVYQAINRITDYPIELVKYQLSAKGQGKDALGQVDIVVDHKGRRFHGVGLA
TDIVESSAKALVHVLNNIWRAHQVEKEKQRLQQNNQEMV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=298, Percent_Identity=25.1677852348993, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1786261, Length=517, Percent_Identity=82.3984526112186, Blast_Score=904, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324682, Length=536, Percent_Identity=25.7462686567164, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324225, Length=535, Percent_Identity=25.607476635514, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6320071, Length=359, Percent_Identity=27.2980501392758, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320019, Length=359, Percent_Identity=27.2980501392758, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 57372; Mature: 57241

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDIMEVGFPVSSPGDFESVRT
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHH
IAQQVKNSRVCALARCVDKDIDVAAEALRIAEAFRIHVFLATSTLHIESKLKRSFDDVLA
HHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEHHHHHHHHHHHHHHHH
MAVHSVKRARNYTDDVEFSCEDAGRTPIDNLCRVVEAAITAGATTINIPDTVGYTPPYQF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
GGIITDLYERVPNIDKAIISVHCHDDLGMSVANSITAVQAGARQVEGTINGLGERAGNCS
CHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LEEVIMAIKVRHEMLGVHTNINHQEIYRTSQLVSKICNMPIPGNKAIVGSNAFAHSSGIH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGVLKNRENYEIMTPESIGLKEVQLNLTSRSGRAAVKHRMEEMGYQDKDYNLDSLYDAF
CCCCCCCCCCCEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFINKQQEEPEYYRLDYFSVQSGSSVMATASVKLVCGEEIKS
HHHHCCCCCEEECCHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEEEEEEEEECHHHHH
EAATGNGPVDAVYQAINRITDYPIELVKYQLSAKGQGKDALGQVDIVVDHKGRRFHGVGL
HCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEECCCH
ATDIVESSAKALVHVLNNIWRAHQVEKEKQRLQQNNQEMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDIMEVGFPVSSPGDFESVRT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHH
IAQQVKNSRVCALARCVDKDIDVAAEALRIAEAFRIHVFLATSTLHIESKLKRSFDDVLA
HHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEHHHHHHHHHHHHHHHH
MAVHSVKRARNYTDDVEFSCEDAGRTPIDNLCRVVEAAITAGATTINIPDTVGYTPPYQF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
GGIITDLYERVPNIDKAIISVHCHDDLGMSVANSITAVQAGARQVEGTINGLGERAGNCS
CHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LEEVIMAIKVRHEMLGVHTNINHQEIYRTSQLVSKICNMPIPGNKAIVGSNAFAHSSGIH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGVLKNRENYEIMTPESIGLKEVQLNLTSRSGRAAVKHRMEEMGYQDKDYNLDSLYDAF
CCCCCCCCCCCEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFINKQQEEPEYYRLDYFSVQSGSSVMATASVKLVCGEEIKS
HHHHCCCCCEEECCHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEEEEEEEEECHHHHH
EAATGNGPVDAVYQAINRITDYPIELVKYQLSAKGQGKDALGQVDIVVDHKGRRFHGVGL
HCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEECCCH
ATDIVESSAKALVHVLNNIWRAHQVEKEKQRLQQNNQEMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA