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The map label for this gene is leuC [H]

Identifier: 22127521

GI number: 22127521

Start: 4044813

End: 4046312

Strand: Direct

Name: leuC [H]

Synonym: y3647

Alternate gene names: 22127521

Gene position: 4044813-4046312 (Clockwise)

Preceding gene: 161484746

Following gene: 22127522

Centisome position: 87.92

GC content: 53.6

Gene sequence:

>1500_bases
ATGGTCACGCAATCGGCACTCACGAAATGGGCGATATCATCGCTAAATTCGTGGTTGAAGGGGTATAAAATGGGCACAAC
ATCATCTCAGAGCAAAACGTTATACCAAAAATTGTACGATGCGCACATTGTGCATGAAGCCCCCAATGAGACCCCATTGC
TGTATATCGACCGCCATTTAGTGCATGAGGTCACCTCACCGCAAGCCTTTGATGGTTTGCGTGCCATGGGCCGCCCGGTG
CGTCAACCGGGTAAGACTTTCGCTACCATGGATCACAACGTTTCTACCCAAACAAAAGACATTAACGCCAGTGGTGAGAT
GGCCCGTATTCAGATGCAAGAGTTAATTAAAAACTGCGCTGAATTCGGTGTCTCATTGTATGACCTGAACCACCCTTTCC
AAGGGATCGTGCATGTCATTGGCCCTGAGCAAGGCATGACGTTACCGGGCATGACCATCGTTTGTGGTGACTCACATACC
GCCACTCACGGCGCATTCGGCTCCTTGGCCTTTGGTATCGGCACCTCAGAAGTTGAGCATGTGTTGGCGACCCAAACCCT
GAAGCAGGGGCGAGCCAAAACCATGAGAATCGAAGTGAATGGCACTGTCGGCGCGGGTATTACGGCTAAAGACATCGTGC
TGGCGATTATCGGTAAAACCGGGAGCGCAGGCGGTACTGGCCATGTGGTGGAGTTCTGTGGCAGCGCGATTGAAGCGTTG
AGCATGGAAGGCCGGATGACATTATGCAACATGGCGATCGAAATGGGGGCCAAAGCCGGTTTGGTTGCCCCTGATGACAC
CACCTTTGCCTATCTGAAAGGCCGTCAGTTCGCCCCAACTGGCGAGCAATGGGAACAAGGTGTTGCCTACTGGCGTACCC
TGAAATCTGATGCTGATGCCCAATTCGATACCATCGTGACACTGGATGCCGCTGATATCGCGCCACAGGTGACGTGGGGA
ACCAATCCGGGTCAGGTCATTGCGGTTAATCAAATCATTCCTGCCCCTGAGTCTTTCAGCGATCCGGTCGAACGTGCATC
AGCAGAGAAAGCCTTAGCTTATATGGACTTGCGCCCCGGTATTAAATTGACTGAAGTGGCGATTGATAAAGTCTTTATCG
GCTCTTGCACTAACTCACGTATTGAAGATTTACGTGCGGCAGCGGCTATTGCCCAAGGGCGTAAAGTCGCCAAGGGTGTG
CAAGCTATCGTGGTGCCAGGCTCCGGCCCGGTGAAAGCGCAGGCAGAAGCGGAAGGTTTGGATAAGATCTTTATCGCCGC
AGGTTTTGAGTGGCGTCTGCCCGGTTGCTCGATGTGTTTGGCCATGAATAATGACCGCCTGGAGCCCGGCGAACGTTGTG
CCTCCACCAGCAACCGTAATTTTGAAGGCCGCCAAGGCCGTGGGGGGCGCACACATTTGGTCAGCCCAGCCATGGCCGCC
GCTGCCGCCGTCAGTGGTCATTTTGCTGATGTTCGCGAATTATCCGCCACCACCCACTAA

Upstream 100 bases:

>100_bases
CTGCGTTTTAGCTTAGGTAAAGATGATGCCGCTGACGCCATTGAGCGCGCGATCAACCAGGCATTGGAGCAAGGTTATCG
CACTGCGGATTTAGCCGGGG

Downstream 100 bases:

>100_bases
CCGGAGACACCACCATGGCAAAATTTATTCAACACATTGGTTTGGTTGCGCCGTTAGATGCAGCAAACGTCGATACCGAC
GCGATTATCCCTAAGCAGTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MVTQSALTKWAISSLNSWLKGYKMGTTSSQSKTLYQKLYDAHIVHEAPNETPLLYIDRHLVHEVTSPQAFDGLRAMGRPV
RQPGKTFATMDHNVSTQTKDINASGEMARIQMQELIKNCAEFGVSLYDLNHPFQGIVHVIGPEQGMTLPGMTIVCGDSHT
ATHGAFGSLAFGIGTSEVEHVLATQTLKQGRAKTMRIEVNGTVGAGITAKDIVLAIIGKTGSAGGTGHVVEFCGSAIEAL
SMEGRMTLCNMAIEMGAKAGLVAPDDTTFAYLKGRQFAPTGEQWEQGVAYWRTLKSDADAQFDTIVTLDAADIAPQVTWG
TNPGQVIAVNQIIPAPESFSDPVERASAEKALAYMDLRPGIKLTEVAIDKVFIGSCTNSRIEDLRAAAAIAQGRKVAKGV
QAIVVPGSGPVKAQAEAEGLDKIFIAAGFEWRLPGCSMCLAMNNDRLEPGERCASTSNRNFEGRQGRGGRTHLVSPAMAA
AAAVSGHFADVRELSATTH

Sequences:

>Translated_499_residues
MVTQSALTKWAISSLNSWLKGYKMGTTSSQSKTLYQKLYDAHIVHEAPNETPLLYIDRHLVHEVTSPQAFDGLRAMGRPV
RQPGKTFATMDHNVSTQTKDINASGEMARIQMQELIKNCAEFGVSLYDLNHPFQGIVHVIGPEQGMTLPGMTIVCGDSHT
ATHGAFGSLAFGIGTSEVEHVLATQTLKQGRAKTMRIEVNGTVGAGITAKDIVLAIIGKTGSAGGTGHVVEFCGSAIEAL
SMEGRMTLCNMAIEMGAKAGLVAPDDTTFAYLKGRQFAPTGEQWEQGVAYWRTLKSDADAQFDTIVTLDAADIAPQVTWG
TNPGQVIAVNQIIPAPESFSDPVERASAEKALAYMDLRPGIKLTEVAIDKVFIGSCTNSRIEDLRAAAAIAQGRKVAKGV
QAIVVPGSGPVKAQAEAEGLDKIFIAAGFEWRLPGCSMCLAMNNDRLEPGERCASTSNRNFEGRQGRGGRTHLVSPAMAA
AAAVSGHFADVRELSATTH
>Mature_499_residues
MVTQSALTKWAISSLNSWLKGYKMGTTSSQSKTLYQKLYDAHIVHEAPNETPLLYIDRHLVHEVTSPQAFDGLRAMGRPV
RQPGKTFATMDHNVSTQTKDINASGEMARIQMQELIKNCAEFGVSLYDLNHPFQGIVHVIGPEQGMTLPGMTIVCGDSHT
ATHGAFGSLAFGIGTSEVEHVLATQTLKQGRAKTMRIEVNGTVGAGITAKDIVLAIIGKTGSAGGTGHVVEFCGSAIEAL
SMEGRMTLCNMAIEMGAKAGLVAPDDTTFAYLKGRQFAPTGEQWEQGVAYWRTLKSDADAQFDTIVTLDAADIAPQVTWG
TNPGQVIAVNQIIPAPESFSDPVERASAEKALAYMDLRPGIKLTEVAIDKVFIGSCTNSRIEDLRAAAAIAQGRKVAKGV
QAIVVPGSGPVKAQAEAEGLDKIFIAAGFEWRLPGCSMCLAMNNDRLEPGERCASTSNRNFEGRQGRGGRTHLVSPAMAA
AAAVSGHFADVRELSATTH

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=412, Percent_Identity=25.9708737864078, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI8659555, Length=396, Percent_Identity=26.5151515151515, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI41352693, Length=381, Percent_Identity=24.9343832020997, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=85.9913793103448, Blast_Score=827, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=486, Percent_Identity=25.1028806584362, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI2367097, Length=375, Percent_Identity=27.4666666666667, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=412, Percent_Identity=27.4271844660194, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=412, Percent_Identity=27.4271844660194, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=307, Percent_Identity=28.3387622149837, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=464, Percent_Identity=26.0775862068966, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=476, Percent_Identity=59.2436974789916, Blast_Score=583, Evalue=1e-167,
Organism=Saccharomyces cerevisiae, GI6320440, Length=482, Percent_Identity=25.9336099585062, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323335, Length=413, Percent_Identity=26.634382566586, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=405, Percent_Identity=27.1604938271605, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI281365315, Length=411, Percent_Identity=24.8175182481752, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17864292, Length=411, Percent_Identity=24.8175182481752, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161076999, Length=411, Percent_Identity=24.8175182481752, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28571643, Length=411, Percent_Identity=25.3041362530414, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24645686, Length=455, Percent_Identity=26.5934065934066, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17137564, Length=455, Percent_Identity=24.6153846153846, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 53185; Mature: 53185

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTQSALTKWAISSLNSWLKGYKMGTTSSQSKTLYQKLYDAHIVHEAPNETPLLYIDRHL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCEEEECHHH
VHEVTSPQAFDGLRAMGRPVRQPGKTFATMDHNVSTQTKDINASGEMARIQMQELIKNCA
HHHCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EFGVSLYDLNHPFQGIVHVIGPEQGMTLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEH
HHCCEEEECCCCHHHHEEEECCCCCCCCCCEEEEECCCCCCCCCCCHHHEECCCHHHHHH
VLATQTLKQGRAKTMRIEVNGTVGAGITAKDIVLAIIGKTGSAGGTGHVVEFCGSAIEAL
HHHHHHHHCCCCEEEEEEECCCCCCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHH
SMEGRMTLCNMAIEMGAKAGLVAPDDTTFAYLKGRQFAPTGEQWEQGVAYWRTLKSDADA
CCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
QFDTIVTLDAADIAPQVTWGTNPGQVIAVNQIIPAPESFSDPVERASAEKALAYMDLRPG
CEEEEEEEEHHHCCCEEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHEEEECCCC
IKLTEVAIDKVFIGSCTNSRIEDLRAAAAIAQGRKVAKGVQAIVVPGSGPVKAQAEAEGL
CEEHHEEHHHEEECCCCCHHHHHHHHHHHHHHCHHHHCCCEEEEECCCCCCCCCHHHCCC
DKIFIAAGFEWRLPGCSMCLAMNNDRLEPGERCASTSNRNFEGRQGRGGRTHLVSPAMAA
CEEEEEECCEEECCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCEECCHHHHH
AAAVSGHFADVRELSATTH
HHHHHCHHHHHHHHHCCCC
>Mature Secondary Structure
MVTQSALTKWAISSLNSWLKGYKMGTTSSQSKTLYQKLYDAHIVHEAPNETPLLYIDRHL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCEEEECHHH
VHEVTSPQAFDGLRAMGRPVRQPGKTFATMDHNVSTQTKDINASGEMARIQMQELIKNCA
HHHCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EFGVSLYDLNHPFQGIVHVIGPEQGMTLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEH
HHCCEEEECCCCHHHHEEEECCCCCCCCCCEEEEECCCCCCCCCCCHHHEECCCHHHHHH
VLATQTLKQGRAKTMRIEVNGTVGAGITAKDIVLAIIGKTGSAGGTGHVVEFCGSAIEAL
HHHHHHHHCCCCEEEEEEECCCCCCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHH
SMEGRMTLCNMAIEMGAKAGLVAPDDTTFAYLKGRQFAPTGEQWEQGVAYWRTLKSDADA
CCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
QFDTIVTLDAADIAPQVTWGTNPGQVIAVNQIIPAPESFSDPVERASAEKALAYMDLRPG
CEEEEEEEEHHHCCCEEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHEEEECCCC
IKLTEVAIDKVFIGSCTNSRIEDLRAAAAIAQGRKVAKGVQAIVVPGSGPVKAQAEAEGL
CEEHHEEHHHEEECCCCCHHHHHHHHHHHHHHCHHHHCCCEEEEECCCCCCCCCHHHCCC
DKIFIAAGFEWRLPGCSMCLAMNNDRLEPGERCASTSNRNFEGRQGRGGRTHLVSPAMAA
CEEEEEECCEEECCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCEECCHHHHH
AAAVSGHFADVRELSATTH
HHHHHCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA