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The map label for this gene is slt [H]

Identifier: 161484740

GI number: 161484740

Start: 4140685

End: 4142604

Strand: Reverse

Name: slt [H]

Synonym: y3727

Alternate gene names: 161484740

Gene position: 4142604-4140685 (Counterclockwise)

Preceding gene: 22127608

Following gene: 22127600

Centisome position: 90.04

GC content: 51.72

Gene sequence:

>1920_bases
ATGGGTAAGTGGCGATATCTGGCGATTGGCGTGTGTCTGATGACGACTTCAGGAGTGACATTCGCCGATTCTCTTGATGC
TCAGCGGCAGCGCTATCAGCAAGTTAAATTGGCTTGGGACAGCAATCAGATGGATACCGTGGCACAACTGATGCCAACGT
TACGTGATTATCCTCTGTACCCTTATTTGGAATACCGTCAGTTGACGCAAGATTTAAGCCAGGTGAGTACGGCGCAAGTG
AAGGATTTTCTGAACCGTCACCCCACATTACCACCGGCGAAATCACTGCCATCTCGTTTTGTGAATGAATTGGCTCGCCG
CGAAGATTGGCGCGGGTTGTTAACGTTCAGCCCGACAGCGCCTAAACCGGTTGCCGCCCGTTGTAACTATTACTATGCCA
AATGGGCCACTGGTGAGCAGTCTGTCGCTTGGGAAGGGGCCAGTGAAATCTGGCTCAATGGCCAGTCATTACCGGGCAGT
TGCGATAAATTATTCAGTGTCTGGCAACAAGCCGGGCACCAGACACCATTGGCAACACTGGCGCGTATGAAGCTCGCGCT
TAAAGAGGGTAACGCCAGCCTGGTGAGTTACTTACTCAAACAACTGCCCGCAGATTACCAAACCATGGGCACTGCACTGG
CAAAATTGCAAAGTGATCCCAGTAGTGTAGAAAGCTTTGCCCGCACCGTCGGGCCGACCGATTTTACCCGTTCAGCGACA
ACAATTGCTTTCACCCGTTTGGCTCGACAGGATGTGGAAAATGCGCGATTGATGATCCCATCATTAGTACGGTTACAAAA
AATGAGTGACAGCGAGAAACTTGAGCTGGAAGAGGCCGTCGCCTGGCGTTTGATGGGTAATGATGCGACTTACGAACAAG
CTAAATGGCGGGATCAAGTTGCGTTACGCAGCCACTCAACCTCGCTACTGGAGCGTCGTATCCGTATGTCATTGGGCAGT
GGTGACCGGCAGGGGCTGGCAACCTGGCTGGCTCGTCTCCCGGCGGAGGCGCAAGATAAAGATGAGTGGCGTTATTGGCG
TGCATCGTTGTTGTTGGAACAAGGAAAAAAAGCGGAAGGTGAGGCAATACTGCGTAGCCTGACGAAAGAACGGGGTTTCT
ATCCGATGGTGGCCGCCCAAAAACTGAATATTCCTTACCCGATTAAGGTTCAGGTGGCGGCCAAGCCAGATGTGTCGCTG
GCGCAACGGCCTGAAATCGCCCGGGTTCGCGAGTTAATGTATTGGAATATGGATAACCAAGCGCGCGCTGAATGGAGCTA
TTTGGTGGCCAGTCGCAGTAAAGCTGAGCAAGAGGCTCTGGCACGCTATGCCTTTGACCAAAAATGGGCTGATCTGAGTG
TGCAAGCCACTATTGTTGCCAAGCTTTGGGATCATCTGGAGGAGCGCTTCCCAATGGCATGGACCAAAGAGTTCCGCCAG
TTTACGGAGGATAAGGGGATCACGCCGAGTTATGCGATGGCGATTGCACGTCAGGAAAGCGCCTGGAATCCAAAAGCACA
ATCACCGGTAGGCGCAACGGGATTAATGCAGGTGATGCCGCGTACTGCAGAACATACCGTAAAACGGAATAATATCGCTG
GGTATCTGAATAGCAGCCAACTCCTTGATCCGGTGACTAACATTGAGATAGGAACCTACTATCTGGAAGAGGTTTATCAG
CAATTCGGGCGTAACCGTATTTTATCCAGTGCGGCTTATAATGCAGGTCCGTCACGGGTGAATACTTGGTTGGGTAACAG
TGGTGGGCAGGTGGATGCGGTGGCTTTCATTGAAAGTATCCCATTCTCTGAAACGCGCGGCTACGTGAAGAATGTGTTGG
CTTATGACGCTTTCTATCGTCACTTTATGAATCGCCCCGCAAAAGTGCTGAGTGACGCCGAGTGGCAGAGGCGTTATTGA

Upstream 100 bases:

>100_bases
TGCGGCAAGAAAGAAGATGAAACCGTAGTCGGTAAAACGCTCGGTAAAAAATCGTGGCCGGTAAAATGTAGTGCCAACAA
CCAGTGAGGAACAGGTAAAT

Downstream 100 bases:

>100_bases
TCGATATCTATTGTTTTGATCGATACCTATTGTTTTGATCGATACCTATTGTCTTTGACGTTGCAGGTGTGTTGGCGGTG
TTAGTGACTTGCTCATCCAC

Product: lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MGKWRYLAIGVCLMTTSGVTFADSLDAQRQRYQQVKLAWDSNQMDTVAQLMPTLRDYPLYPYLEYRQLTQDLSQVSTAQV
KDFLNRHPTLPPAKSLPSRFVNELARREDWRGLLTFSPTAPKPVAARCNYYYAKWATGEQSVAWEGASEIWLNGQSLPGS
CDKLFSVWQQAGHQTPLATLARMKLALKEGNASLVSYLLKQLPADYQTMGTALAKLQSDPSSVESFARTVGPTDFTRSAT
TIAFTRLARQDVENARLMIPSLVRLQKMSDSEKLELEEAVAWRLMGNDATYEQAKWRDQVALRSHSTSLLERRIRMSLGS
GDRQGLATWLARLPAEAQDKDEWRYWRASLLLEQGKKAEGEAILRSLTKERGFYPMVAAQKLNIPYPIKVQVAAKPDVSL
AQRPEIARVRELMYWNMDNQARAEWSYLVASRSKAEQEALARYAFDQKWADLSVQATIVAKLWDHLEERFPMAWTKEFRQ
FTEDKGITPSYAMAIARQESAWNPKAQSPVGATGLMQVMPRTAEHTVKRNNIAGYLNSSQLLDPVTNIEIGTYYLEEVYQ
QFGRNRILSSAAYNAGPSRVNTWLGNSGGQVDAVAFIESIPFSETRGYVKNVLAYDAFYRHFMNRPAKVLSDAEWQRRY

Sequences:

>Translated_639_residues
MGKWRYLAIGVCLMTTSGVTFADSLDAQRQRYQQVKLAWDSNQMDTVAQLMPTLRDYPLYPYLEYRQLTQDLSQVSTAQV
KDFLNRHPTLPPAKSLPSRFVNELARREDWRGLLTFSPTAPKPVAARCNYYYAKWATGEQSVAWEGASEIWLNGQSLPGS
CDKLFSVWQQAGHQTPLATLARMKLALKEGNASLVSYLLKQLPADYQTMGTALAKLQSDPSSVESFARTVGPTDFTRSAT
TIAFTRLARQDVENARLMIPSLVRLQKMSDSEKLELEEAVAWRLMGNDATYEQAKWRDQVALRSHSTSLLERRIRMSLGS
GDRQGLATWLARLPAEAQDKDEWRYWRASLLLEQGKKAEGEAILRSLTKERGFYPMVAAQKLNIPYPIKVQVAAKPDVSL
AQRPEIARVRELMYWNMDNQARAEWSYLVASRSKAEQEALARYAFDQKWADLSVQATIVAKLWDHLEERFPMAWTKEFRQ
FTEDKGITPSYAMAIARQESAWNPKAQSPVGATGLMQVMPRTAEHTVKRNNIAGYLNSSQLLDPVTNIEIGTYYLEEVYQ
QFGRNRILSSAAYNAGPSRVNTWLGNSGGQVDAVAFIESIPFSETRGYVKNVLAYDAFYRHFMNRPAKVLSDAEWQRRY
>Mature_638_residues
GKWRYLAIGVCLMTTSGVTFADSLDAQRQRYQQVKLAWDSNQMDTVAQLMPTLRDYPLYPYLEYRQLTQDLSQVSTAQVK
DFLNRHPTLPPAKSLPSRFVNELARREDWRGLLTFSPTAPKPVAARCNYYYAKWATGEQSVAWEGASEIWLNGQSLPGSC
DKLFSVWQQAGHQTPLATLARMKLALKEGNASLVSYLLKQLPADYQTMGTALAKLQSDPSSVESFARTVGPTDFTRSATT
IAFTRLARQDVENARLMIPSLVRLQKMSDSEKLELEEAVAWRLMGNDATYEQAKWRDQVALRSHSTSLLERRIRMSLGSG
DRQGLATWLARLPAEAQDKDEWRYWRASLLLEQGKKAEGEAILRSLTKERGFYPMVAAQKLNIPYPIKVQVAAKPDVSLA
QRPEIARVRELMYWNMDNQARAEWSYLVASRSKAEQEALARYAFDQKWADLSVQATIVAKLWDHLEERFPMAWTKEFRQF
TEDKGITPSYAMAIARQESAWNPKAQSPVGATGLMQVMPRTAEHTVKRNNIAGYLNSSQLLDPVTNIEIGTYYLEEVYQQ
FGRNRILSSAAYNAGPSRVNTWLGNSGGQVDAVAFIESIPFSETRGYVKNVLAYDAFYRHFMNRPAKVLSDAEWQRRY

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=637, Percent_Identity=63.8932496075353, Blast_Score=859, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 72636; Mature: 72505

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00922 TRANSGLYCOSYLASE ; PS01258 BH2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKWRYLAIGVCLMTTSGVTFADSLDAQRQRYQQVKLAWDSNQMDTVAQLMPTLRDYPLY
CCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCC
PYLEYRQLTQDLSQVSTAQVKDFLNRHPTLPPAKSLPSRFVNELARREDWRGLLTFSPTA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCC
PKPVAARCNYYYAKWATGEQSVAWEGASEIWLNGQSLPGSCDKLFSVWQQAGHQTPLATL
CCCHHHHCCEEEEEECCCCCCEEECCCHHEEECCCCCCCHHHHHHHHHHHCCCCCHHHHH
ARMKLALKEGNASLVSYLLKQLPADYQTMGTALAKLQSDPSSVESFARTVGPTDFTRSAT
HHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHH
TIAFTRLARQDVENARLMIPSLVRLQKMSDSEKLELEEAVAWRLMGNDATYEQAKWRDQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ALRSHSTSLLERRIRMSLGSGDRQGLATWLARLPAEAQDKDEWRYWRASLLLEQGKKAEG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHH
EAILRSLTKERGFYPMVAAQKLNIPYPIKVQVAAKPDVSLAQRPEIARVRELMYWNMDNQ
HHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCCCCHHCCCHHHHHHHHHHCCCCCH
ARAEWSYLVASRSKAEQEALARYAFDQKWADLSVQATIVAKLWDHLEERFPMAWTKEFRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
FTEDKGITPSYAMAIARQESAWNPKAQSPVGATGLMQVMPRTAEHTVKRNNIAGYLNSSQ
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCEECCCHH
LLDPVTNIEIGTYYLEEVYQQFGRNRILSSAAYNAGPSRVNTWLGNSGGQVDAVAFIESI
HHCCHHCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHC
PFSETRGYVKNVLAYDAFYRHFMNRPAKVLSDAEWQRRY
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCC
>Mature Secondary Structure 
GKWRYLAIGVCLMTTSGVTFADSLDAQRQRYQQVKLAWDSNQMDTVAQLMPTLRDYPLY
CCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCC
PYLEYRQLTQDLSQVSTAQVKDFLNRHPTLPPAKSLPSRFVNELARREDWRGLLTFSPTA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCC
PKPVAARCNYYYAKWATGEQSVAWEGASEIWLNGQSLPGSCDKLFSVWQQAGHQTPLATL
CCCHHHHCCEEEEEECCCCCCEEECCCHHEEECCCCCCCHHHHHHHHHHHCCCCCHHHHH
ARMKLALKEGNASLVSYLLKQLPADYQTMGTALAKLQSDPSSVESFARTVGPTDFTRSAT
HHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHH
TIAFTRLARQDVENARLMIPSLVRLQKMSDSEKLELEEAVAWRLMGNDATYEQAKWRDQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ALRSHSTSLLERRIRMSLGSGDRQGLATWLARLPAEAQDKDEWRYWRASLLLEQGKKAEG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHH
EAILRSLTKERGFYPMVAAQKLNIPYPIKVQVAAKPDVSLAQRPEIARVRELMYWNMDNQ
HHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCCCCHHCCCHHHHHHHHHHCCCCCH
ARAEWSYLVASRSKAEQEALARYAFDQKWADLSVQATIVAKLWDHLEERFPMAWTKEFRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
FTEDKGITPSYAMAIARQESAWNPKAQSPVGATGLMQVMPRTAEHTVKRNNIAGYLNSSQ
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCEECCCHH
LLDPVTNIEIGTYYLEEVYQQFGRNRILSSAAYNAGPSRVNTWLGNSGGQVDAVAFIESI
HHCCHHCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHC
PFSETRGYVKNVLAYDAFYRHFMNRPAKVLSDAEWQRRY
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]