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The map label for this gene is trpR [H]

Identifier: 22127600

GI number: 22127600

Start: 4140038

End: 4140439

Strand: Reverse

Name: trpR [H]

Synonym: y3726

Alternate gene names: 22127600

Gene position: 4140439-4140038 (Counterclockwise)

Preceding gene: 161484740

Following gene: 22127598

Centisome position: 89.99

GC content: 50.0

Gene sequence:

>402_bases
GTGGTTATCATGACAAAACAATTTTTGACTGACGAAAAACAAGTGTCGGATTCACTTAATAGGCAGACGGCTGGGAACCC
TTACTCTGCCGCTGATCCGGCATTGTCTGCCGAAGATAATCAGCACTGGTTGAGCTTTGTCGCGCTGTTGCAAAACGCGA
TTACACAAGATCTTCATCTGCCTTTACTGCAACTGATGCTAACACCTGATGAGCGAACCGCCTTGGGGACGCGTGTGCGC
ATTATTGAAGAGTTAATGCGCGGTGAGCTAAGCCAGCGTGAGCTGAAAAGTCAGTTGGGTGCAGGTATTGCGACGATCAC
CCGTGGCTCTAATAGCTTGAAAACGGCCCCACCACAGCTGAAAAGCTGGCTCGAAGCCCAGTTGCTAGCCAACAAACGCT
AA

Upstream 100 bases:

>100_bases
TGTCGTAGTTAACTAAAATGGCCGCTTTTTGCCTCTTGAAATCTAGTGAGTATAGATAACTCTATGGTATGCTTGTTGTA
CTAGTTAAATAGTACTTTGG

Downstream 100 bases:

>100_bases
ATGGCTGGTTTAGAGGGCGATGGGCGTTGGTAAATCTCATTGTGGAACGGCACCAGTGCCAACAAGAGGGCCTGGTGATA
GACACTGGTTCGAGTTAATT

Product: Trp operon repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MVIMTKQFLTDEKQVSDSLNRQTAGNPYSAADPALSAEDNQHWLSFVALLQNAITQDLHLPLLQLMLTPDERTALGTRVR
IIEELMRGELSQRELKSQLGAGIATITRGSNSLKTAPPQLKSWLEAQLLANKR

Sequences:

>Translated_133_residues
MVIMTKQFLTDEKQVSDSLNRQTAGNPYSAADPALSAEDNQHWLSFVALLQNAITQDLHLPLLQLMLTPDERTALGTRVR
IIEELMRGELSQRELKSQLGAGIATITRGSNSLKTAPPQLKSWLEAQLLANKR
>Mature_133_residues
MVIMTKQFLTDEKQVSDSLNRQTAGNPYSAADPALSAEDNQHWLSFVALLQNAITQDLHLPLLQLMLTPDERTALGTRVR
IIEELMRGELSQRELKSQLGAGIATITRGSNSLKTAPPQLKSWLEAQLLANKR

Specific function: This protein is an aporepressor. When complexed with L- tryptophan it binds the operator region of the trp operon (5'- ACTAGT-'3') and prevents the initiation of transcription. The complex also regulates trp repressor biosynthesis by binding to its regula

COG id: COG2973

COG function: function code K; Trp operon repressor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpR family [H]

Homologues:

Organism=Escherichia coli, GI1790854, Length=104, Percent_Identity=70.1923076923077, Blast_Score=126, Evalue=6e-31,

Paralogues:

None

Copy number: 250-750 50-300 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000831
- InterPro:   IPR013335
- InterPro:   IPR010921
- ProDom:   PD012582 [H]

Pfam domain/function: PF01371 Trp_repressor [H]

EC number: NA

Molecular weight: Translated: 14779; Mature: 14779

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIMTKQFLTDEKQVSDSLNRQTAGNPYSAADPALSAEDNQHWLSFVALLQNAITQDLHL
CEEECHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PLLQLMLTPDERTALGTRVRIIEELMRGELSQRELKSQLGAGIATITRGSNSLKTAPPQL
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHEEECCCCCCCCCCHHH
KSWLEAQLLANKR
HHHHHHHHHHCCC
>Mature Secondary Structure
MVIMTKQFLTDEKQVSDSLNRQTAGNPYSAADPALSAEDNQHWLSFVALLQNAITQDLHL
CEEECHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PLLQLMLTPDERTALGTRVRIIEELMRGELSQRELKSQLGAGIATITRGSNSLKTAPPQL
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHEEECCCCCCCCCCHHH
KSWLEAQLLANKR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]