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The map label for this gene is rep

Identifier: 21672830

GI number: 21672830

Start: 628697

End: 630673

Strand: Reverse

Name: rep

Synonym: BUsg574

Alternate gene names: 21672830

Gene position: 630673-628697 (Counterclockwise)

Preceding gene: 21672831

Following gene: 21672829

Centisome position: 98.32

GC content: 22.36

Gene sequence:

>1977_bases
ATGTCTCTTAATTTTAATCAAAAAAATGCTATAGAACTCATTAATGGTCCCTGCTTAATATTAGCAGGGGCTGGTTCAGG
AAAAACAAAAGTTATTATTAATAAAATTATTTACTTAATCAATAATTGTCAATATAAACCTGGAAACATTATTGCTGTTA
CTTTCACTAATAAAGCTGCTCATGAAATAAAAGTTCGTCTTGCAAAACATTTAAATTTATTGCAAATAAAAAAAATGATT
ATTTCAACTTTTCATTCTCTTGGATTAGAAATCATTAAAAAAGAAATTAATACATTAAAATTTAACTCTAATTTTTCTTT
ATTTGATGAAAGAGATCAAATGATGTTGTTAAAAAAAATATGCAGTAAAAGTATAAAAAATGATACAAAATTGTTAAAAA
AATTAGTTTTTATGATTTCTTTTTGGAAAAATAAATTTCTCACTCCATTACAAGTTCAATTATCAGCACAATCAAATTTA
GAAAAAGATTTCGCATTTTTTTATAAGCAATACACTTTTCATTTACGAAAATCAAACATATTAGATTTTGATGATTTAAT
TTGTATACCAACAAGTTTATTAAAAAATAATCAAATTATACAAAATCGTTGGCAAAAAAAAATTTCTTATCTATTAGTAG
ACGAATATCAAGATACAAATAATAGTCAATATGAACTAATTAAAATGCTTACTAATGTAAATTCAAATTTTACTTTAGTA
GGAGATGATGATCAATCTATATATTCATGGAGAGGAGCAAAACCACAAAATCTTTTTTTAATAAAAAAAGATTTTCCCAA
CTTAAAAATTATAAAAATGGAACAAAATTATCGCTCTTACGGAAGAATACTTAAAGCAGCAAATAAATTAATCTCAAACA
ATTTACATTATTTTAAAAAAAAATTATTTTCTAATTTAGAATACGGTAACAAAATAAAAGTTATAATTGGAAAAAATGAA
AAAAATGAAGCAGAAAAAATAGCAGATAAAATAATTCATGAATGTTCTAACGATATAATGCAATACAAAGATTATGCTAT
ATTATATCGAGGGAATTACCAATCTCAAATTCTTGAAAAAACTTTCTTAAAAAAAAATATTCCTTATGATATCTCTACAA
ATTCATCATTTTTTTCTCGTCCTGAAATAAAAGATTTATTAAGTTATCTTCGTTTAATTGTAAATCCAGACGATAATCAT
GCATTTATAAGAATTTTAAATATTCCTCATCGCCAAATAGGATTAACTACACTAAATAAATTAGAAGAATTAGCTTCTAA
AAAAAATAAAAGTCTTTTTCAAATTAGTAACGATATAGAAATAAAAAAAATTTTAAGAGAAAGAACAGTAAAAAAAATTA
AAGATTTTATTTATTGGATTAAAAAAATAATCAAATTATCACTTCTTAAAGAAGATATCATTTTAGATAAAATTATTAAT
GATATTAAATATGAACTATGGTTAACTAAAATTTTAAAAGAACCGAAAAAAATTAAAACAAGCATAAATAATATTTACAC
GTTATCAAATTGGTTAAAAGAAATGTTAAGGGGAAATGAATTTGAAAAACCAATGAATTTATTACAAATTGTTAAAAAAA
TGACATTACGAGATATTTTAGAAAAAAAAATACAAATAAATGAAATCCCAAAAAATCGAGTACAATTGATGACTTTGCAT
TCATCTAAAGGATTGGAATTTTCTTCAGTTTTTATAATTGGTATGAATGAGGGAATTTTACCCAATATAAAGAGCATTAA
TAATGATAACATAGAGGAAGAAAGAAGATTAACTTATGTAGGAATGACAAGAGCACGAAAAGAACTATTTTTTACATATT
GTCAAACTAGAATTCAATATGGACAAAAATTATATACAGCACCTAGTAGATTTTTATTTGAATTACCCCAAGAAGATTTA
CAATGGGAAAAAGATGATTATTTAGATGCTTTTCATACAAAAGAAAAAAAAATATAA

Upstream 100 bases:

>100_bases
ATTTGTGTTGCAAAATAAACAATAAATTCTCTAAATTTTACCTTAATTTTTTAAGAATTTTTAAAAATTAATTTTCTAAT
CAATCTGAAATTATTATTTT

Downstream 100 bases:

>100_bases
AAATAGAAAACTTAAAAAAAATCTTAAAAAATAAAAAAATATCTAATTTCAGATAGTTAAAATTATTTAATTTTAGTTTA
AATCAATTCCCAAAAATGAT

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MSLNFNQKNAIELINGPCLILAGAGSGKTKVIINKIIYLINNCQYKPGNIIAVTFTNKAAHEIKVRLAKHLNLLQIKKMI
ISTFHSLGLEIIKKEINTLKFNSNFSLFDERDQMMLLKKICSKSIKNDTKLLKKLVFMISFWKNKFLTPLQVQLSAQSNL
EKDFAFFYKQYTFHLRKSNILDFDDLICIPTSLLKNNQIIQNRWQKKISYLLVDEYQDTNNSQYELIKMLTNVNSNFTLV
GDDDQSIYSWRGAKPQNLFLIKKDFPNLKIIKMEQNYRSYGRILKAANKLISNNLHYFKKKLFSNLEYGNKIKVIIGKNE
KNEAEKIADKIIHECSNDIMQYKDYAILYRGNYQSQILEKTFLKKNIPYDISTNSSFFSRPEIKDLLSYLRLIVNPDDNH
AFIRILNIPHRQIGLTTLNKLEELASKKNKSLFQISNDIEIKKILRERTVKKIKDFIYWIKKIIKLSLLKEDIILDKIIN
DIKYELWLTKILKEPKKIKTSINNIYTLSNWLKEMLRGNEFEKPMNLLQIVKKMTLRDILEKKIQINEIPKNRVQLMTLH
SSKGLEFSSVFIIGMNEGILPNIKSINNDNIEEERRLTYVGMTRARKELFFTYCQTRIQYGQKLYTAPSRFLFELPQEDL
QWEKDDYLDAFHTKEKKI

Sequences:

>Translated_658_residues
MSLNFNQKNAIELINGPCLILAGAGSGKTKVIINKIIYLINNCQYKPGNIIAVTFTNKAAHEIKVRLAKHLNLLQIKKMI
ISTFHSLGLEIIKKEINTLKFNSNFSLFDERDQMMLLKKICSKSIKNDTKLLKKLVFMISFWKNKFLTPLQVQLSAQSNL
EKDFAFFYKQYTFHLRKSNILDFDDLICIPTSLLKNNQIIQNRWQKKISYLLVDEYQDTNNSQYELIKMLTNVNSNFTLV
GDDDQSIYSWRGAKPQNLFLIKKDFPNLKIIKMEQNYRSYGRILKAANKLISNNLHYFKKKLFSNLEYGNKIKVIIGKNE
KNEAEKIADKIIHECSNDIMQYKDYAILYRGNYQSQILEKTFLKKNIPYDISTNSSFFSRPEIKDLLSYLRLIVNPDDNH
AFIRILNIPHRQIGLTTLNKLEELASKKNKSLFQISNDIEIKKILRERTVKKIKDFIYWIKKIIKLSLLKEDIILDKIIN
DIKYELWLTKILKEPKKIKTSINNIYTLSNWLKEMLRGNEFEKPMNLLQIVKKMTLRDILEKKIQINEIPKNRVQLMTLH
SSKGLEFSSVFIIGMNEGILPNIKSINNDNIEEERRLTYVGMTRARKELFFTYCQTRIQYGQKLYTAPSRFLFELPQEDL
QWEKDDYLDAFHTKEKKI
>Mature_657_residues
SLNFNQKNAIELINGPCLILAGAGSGKTKVIINKIIYLINNCQYKPGNIIAVTFTNKAAHEIKVRLAKHLNLLQIKKMII
STFHSLGLEIIKKEINTLKFNSNFSLFDERDQMMLLKKICSKSIKNDTKLLKKLVFMISFWKNKFLTPLQVQLSAQSNLE
KDFAFFYKQYTFHLRKSNILDFDDLICIPTSLLKNNQIIQNRWQKKISYLLVDEYQDTNNSQYELIKMLTNVNSNFTLVG
DDDQSIYSWRGAKPQNLFLIKKDFPNLKIIKMEQNYRSYGRILKAANKLISNNLHYFKKKLFSNLEYGNKIKVIIGKNEK
NEAEKIADKIIHECSNDIMQYKDYAILYRGNYQSQILEKTFLKKNIPYDISTNSSFFSRPEIKDLLSYLRLIVNPDDNHA
FIRILNIPHRQIGLTTLNKLEELASKKNKSLFQISNDIEIKKILRERTVKKIKDFIYWIKKIIKLSLLKEDIILDKIIND
IKYELWLTKILKEPKKIKTSINNIYTLSNWLKEMLRGNEFEKPMNLLQIVKKMTLRDILEKKIQINEIPKNRVQLMTLHS
SKGLEFSSVFIIGMNEGILPNIKSINNDNIEEERRLTYVGMTRARKELFFTYCQTRIQYGQKLYTAPSRFLFELPQEDLQ
WEKDDYLDAFHTKEKKI

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI48994965, Length=645, Percent_Identity=52.4031007751938, Blast_Score=700, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=643, Percent_Identity=35.9253499222395, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6322369, Length=723, Percent_Identity=28.2157676348548, Blast_Score=198, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): REP_BUCAP (O51889)

Other databases:

- EMBL:   AF008210
- EMBL:   AE013218
- RefSeq:   NP_660897.1
- ProteinModelPortal:   O51889
- SMR:   O51889
- EnsemblBacteria:   EBBUCT00000000414
- GeneID:   1005623
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg574
- GeneTree:   EBGT00050000007704
- HOGENOM:   HBG467281
- OMA:   MSLHSSK
- ProtClustDB:   CLSK316258
- BioCyc:   BAPH198804:BUSG574-MONOMER
- GO:   GO:0005737
- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016
- Gene3D:   G3DSA:1.10.10.160
- PANTHER:   PTHR11070
- TIGRFAMs:   TIGR01074

Pfam domain/function: PF00580 UvrD-helicase

EC number: =3.6.4.12

Molecular weight: Translated: 77510; Mature: 77379

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS51198 UVRD_HELICASE_ATP_BIND; PS51217 UVRD_HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNFNQKNAIELINGPCLILAGAGSGKTKVIINKIIYLINNCQYKPGNIIAVTFTNKAA
CCCCCCCCCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHH
HEIKVRLAKHLNLLQIKKMIISTFHSLGLEIIKKEINTLKFNSNFSLFDERDQMMLLKKI
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHH
CSKSIKNDTKLLKKLVFMISFWKNKFLTPLQVQLSAQSNLEKDFAFFYKQYTFHLRKSNI
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
LDFDDLICIPTSLLKNNQIIQNRWQKKISYLLVDEYQDTNNSQYELIKMLTNVNSNFTLV
CCCHHHHHHCHHHHCCCHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCCCCCEEEE
GDDDQSIYSWRGAKPQNLFLIKKDFPNLKIIKMEQNYRSYGRILKAANKLISNNLHYFKK
CCCCCHHHHCCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHH
KLFSNLEYGNKIKVIIGKNEKNEAEKIADKIIHECSNDIMQYKDYAILYRGNYQSQILEK
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHH
TFLKKNIPYDISTNSSFFSRPEIKDLLSYLRLIVNPDDNHAFIRILNIPHRQIGLTTLNK
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHH
LEELASKKNKSLFQISNDIEIKKILRERTVKKIKDFIYWIKKIIKLSLLKEDIILDKIIN
HHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIKYELWLTKILKEPKKIKTSINNIYTLSNWLKEMLRGNEFEKPMNLLQIVKKMTLRDIL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
EKKIQINEIPKNRVQLMTLHSSKGLEFSSVFIIGMNEGILPNIKSINNDNIEEERRLTYV
HHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
GMTRARKELFFTYCQTRIQYGQKLYTAPSRFLFELPQEDLQWEKDDYLDAFHTKEKKI
HHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHCCHHHCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLNFNQKNAIELINGPCLILAGAGSGKTKVIINKIIYLINNCQYKPGNIIAVTFTNKAA
CCCCCCCCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHH
HEIKVRLAKHLNLLQIKKMIISTFHSLGLEIIKKEINTLKFNSNFSLFDERDQMMLLKKI
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHH
CSKSIKNDTKLLKKLVFMISFWKNKFLTPLQVQLSAQSNLEKDFAFFYKQYTFHLRKSNI
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
LDFDDLICIPTSLLKNNQIIQNRWQKKISYLLVDEYQDTNNSQYELIKMLTNVNSNFTLV
CCCHHHHHHCHHHHCCCHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCCCCCEEEE
GDDDQSIYSWRGAKPQNLFLIKKDFPNLKIIKMEQNYRSYGRILKAANKLISNNLHYFKK
CCCCCHHHHCCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHH
KLFSNLEYGNKIKVIIGKNEKNEAEKIADKIIHECSNDIMQYKDYAILYRGNYQSQILEK
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHH
TFLKKNIPYDISTNSSFFSRPEIKDLLSYLRLIVNPDDNHAFIRILNIPHRQIGLTTLNK
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHH
LEELASKKNKSLFQISNDIEIKKILRERTVKKIKDFIYWIKKIIKLSLLKEDIILDKIIN
HHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIKYELWLTKILKEPKKIKTSINNIYTLSNWLKEMLRGNEFEKPMNLLQIVKKMTLRDIL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
EKKIQINEIPKNRVQLMTLHSSKGLEFSSVFIIGMNEGILPNIKSINNDNIEEERRLTYV
HHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
GMTRARKELFFTYCQTRIQYGQKLYTAPSRFLFELPQEDLQWEKDDYLDAFHTKEKKI
HHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHCCHHHCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9516544; 12089438