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The map label for this gene is lipA

Identifier: 21672539

GI number: 21672539

Start: 295766

End: 296725

Strand: Direct

Name: lipA

Synonym: BUsg259

Alternate gene names: 21672539

Gene position: 295766-296725 (Clockwise)

Preceding gene: 30410818

Following gene: 21672540

Centisome position: 46.11

GC content: 27.4

Gene sequence:

>960_bases
ATGAATAAAAAAAAAGATTCTTTGTTCAAAACAATAAAAAAAAATAACATAATAAATGTTATTACTACTGAAAGTTTTTC
TAAAAAAATAATAAAACTTAATAAACCTGATTGGATAAAAATTAAAATACCTGTCGATACGTTTCGTATTCGTGAAATAA
AAAGTGCTTTACGTAAAAACAATTTACATTCTGTTTGTGAAGAAGCTAATTGTCCAAATCTACCAGAATGTTTTAATCGC
GGAACTGCAACGTTTATGATTCTTGGATCAAGATGTACTCGTAATTGTCCATTTTGTGCTGTATCTCATGGCAAACCTAA
TTCTCTAAATGTCGAAGAACCAAATAATTTAGCAAAAACTATATTTGATATGGGTATTGATTATGTAGTAATCACTTCAG
TTGTAAGAGATGATTTGTATGACGGAGGAGCGCAACATTTTGTTAATTGTATTAAATCAATTAGAAAAAAAAATAAAGTT
AAAATTGAAATATTAGTTCCTGACTTTAGGGGAAGAGTAGAATTAATTTTAAAAATTTTTAATTCAGGATTGCCTGATGT
GTTTAATCATAATGTAGAAAATGTTCCTCGTCTTTATAAAAAAGTACGTCCTGGAGCTGATTATAAAAAATCGTTGTTTC
TATTAGAATCCTTTAAAAAAAAATATTCTAATATTCCTACAAAATCAGGTTTAATGTTAGGTTTAGGAGAAAAAGATACA
GAAATAATTCAAGTAATGAAAGATCTGTATTCAAATGGTGTTACATTGTTAACAGTAGGACAGTATCTGCAACCTAGTAT
TAATCACATTCCAGTACAACGTTATATACCACTTTCAGAATTCAAAAATATAAAAAAAGAAGCATTATCTATCGGTTTTA
CTAATGCATTCTGCGGACCTTTTGTACGTTCATCGTATCATGCTAGTTTTCAAGCAAATAAATTGATTAAAAAAATTTAA

Upstream 100 bases:

>100_bases
ATTTTATTCTTATTAAGAATATAACATTGTATCAAATAATTACCTGTTAAGTTAATAATTACTATGTAAATTAAAATTTC
TGATCGAGACAATTTATTTT

Downstream 100 bases:

>100_bases
TTTTTTTTATTTTAAATAAATGTTTTAAATATTATTTAATTAGCATCTTTATATATTGAAAATATATAAAACTATTTCTA
ATAGAGGTTAAATAGTGTTA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MNKKKDSLFKTIKKNNIINVITTESFSKKIIKLNKPDWIKIKIPVDTFRIREIKSALRKNNLHSVCEEANCPNLPECFNR
GTATFMILGSRCTRNCPFCAVSHGKPNSLNVEEPNNLAKTIFDMGIDYVVITSVVRDDLYDGGAQHFVNCIKSIRKKNKV
KIEILVPDFRGRVELILKIFNSGLPDVFNHNVENVPRLYKKVRPGADYKKSLFLLESFKKKYSNIPTKSGLMLGLGEKDT
EIIQVMKDLYSNGVTLLTVGQYLQPSINHIPVQRYIPLSEFKNIKKEALSIGFTNAFCGPFVRSSYHASFQANKLIKKI

Sequences:

>Translated_319_residues
MNKKKDSLFKTIKKNNIINVITTESFSKKIIKLNKPDWIKIKIPVDTFRIREIKSALRKNNLHSVCEEANCPNLPECFNR
GTATFMILGSRCTRNCPFCAVSHGKPNSLNVEEPNNLAKTIFDMGIDYVVITSVVRDDLYDGGAQHFVNCIKSIRKKNKV
KIEILVPDFRGRVELILKIFNSGLPDVFNHNVENVPRLYKKVRPGADYKKSLFLLESFKKKYSNIPTKSGLMLGLGEKDT
EIIQVMKDLYSNGVTLLTVGQYLQPSINHIPVQRYIPLSEFKNIKKEALSIGFTNAFCGPFVRSSYHASFQANKLIKKI
>Mature_319_residues
MNKKKDSLFKTIKKNNIINVITTESFSKKIIKLNKPDWIKIKIPVDTFRIREIKSALRKNNLHSVCEEANCPNLPECFNR
GTATFMILGSRCTRNCPFCAVSHGKPNSLNVEEPNNLAKTIFDMGIDYVVITSVVRDDLYDGGAQHFVNCIKSIRKKNKV
KIEILVPDFRGRVELILKIFNSGLPDVFNHNVENVPRLYKKVRPGADYKKSLFLLESFKKKYSNIPTKSGLMLGLGEKDT
EIIQVMKDLYSNGVTLLTVGQYLQPSINHIPVQRYIPLSEFKNIKKEALSIGFTNAFCGPFVRSSYHASFQANKLIKKI

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=290, Percent_Identity=44.4827586206897, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI37577164, Length=253, Percent_Identity=45.0592885375494, Blast_Score=216, Evalue=2e-56,
Organism=Escherichia coli, GI1786846, Length=284, Percent_Identity=64.7887323943662, Blast_Score=406, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=39.3728222996516, Blast_Score=201, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6324770, Length=293, Percent_Identity=40.2730375426621, Blast_Score=210, Evalue=2e-55,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=44.4055944055944, Blast_Score=237, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_BUCAP (Q8K9Q2)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660606.1
- ProteinModelPortal:   Q8K9Q2
- EnsemblBacteria:   EBBUCT00000000305
- GeneID:   1005461
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg259
- GeneTree:   EBGT00050000007940
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   BAPH198804:BUSG259-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36290; Mature: 36290

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKKDSLFKTIKKNNIINVITTESFSKKIIKLNKPDWIKIKIPVDTFRIREIKSALRKN
CCCHHHHHHHHHHHCCEEEEEECCCHHHHHEECCCCCEEEEEECCHHHHHHHHHHHHHHC
NLHSVCEEANCPNLPECFNRGTATFMILGSRCTRNCPFCAVSHGKPNSLNVEEPNNLAKT
CHHHHHHHCCCCCCHHHHCCCCEEEEEECCHHCCCCCEEEECCCCCCCCCCCCCHHHHHH
IFDMGIDYVVITSVVRDDLYDGGAQHFVNCIKSIRKKNKVKIEILVPDFRGRVELILKIF
HHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
NSGLPDVFNHNVENVPRLYKKVRPGADYKKSLFLLESFKKKYSNIPTKSGLMLGLGEKDT
HCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHH
EIIQVMKDLYSNGVTLLTVGQYLQPSINHIPVQRYIPLSEFKNIKKEALSIGFTNAFCGP
HHHHHHHHHHHCCEEEEEECHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCHHHHHHH
FVRSSYHASFQANKLIKKI
HHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MNKKKDSLFKTIKKNNIINVITTESFSKKIIKLNKPDWIKIKIPVDTFRIREIKSALRKN
CCCHHHHHHHHHHHCCEEEEEECCCHHHHHEECCCCCEEEEEECCHHHHHHHHHHHHHHC
NLHSVCEEANCPNLPECFNRGTATFMILGSRCTRNCPFCAVSHGKPNSLNVEEPNNLAKT
CHHHHHHHCCCCCCHHHHCCCCEEEEEECCHHCCCCCEEEECCCCCCCCCCCCCHHHHHH
IFDMGIDYVVITSVVRDDLYDGGAQHFVNCIKSIRKKNKVKIEILVPDFRGRVELILKIF
HHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
NSGLPDVFNHNVENVPRLYKKVRPGADYKKSLFLLESFKKKYSNIPTKSGLMLGLGEKDT
HCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHH
EIIQVMKDLYSNGVTLLTVGQYLQPSINHIPVQRYIPLSEFKNIKKEALSIGFTNAFCGP
HHHHHHHHHHHCCEEEEEECHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCHHHHHHH
FVRSSYHASFQANKLIKKI
HHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438