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The map label for this gene is lipB

Identifier: 30410818

GI number: 30410818

Start: 294987

End: 295643

Strand: Direct

Name: lipB

Synonym: BUsg258

Alternate gene names: 30410818

Gene position: 294987-295643 (Clockwise)

Preceding gene: 21672537

Following gene: 21672539

Centisome position: 45.99

GC content: 22.98

Gene sequence:

>657_bases
TTGAATAATAGAATTATTTTGTTTCAAAATTTCGGTTTAAAACATTGGATTGATATGTTTAATAAAATGCATGCTTTTAC
AGAAGTACGCAATATTAATACATATGACGAAATTTGGTTTTTAGAACATTATCCAATTTTTACTCAAGGTCTATTACAAA
AAATAAATACAATTACATATAATAATGATATTCTTCATGATATTCCAATAGTCTCTACAGATAGAGGTGGTCAAATTACA
TATCATGGCCCTGGACAACAAATATTGTATTTTTTAATTGATTTAAAACGTCGAAAAATTACTATTCGTGATCTTATAAA
TATAATGCAAAATTTAATAATAGAAACTTTAAATTATTTTTCTATTAAATCACATATTAAAAAAAATTCTCCTGGAGTTT
ATGTAAATAATAAAAAAATATCTTCTTTAGGATTAAGAGTAAAAAAAGGATTTACATTACATGGTTTGTCATTAAATGTT
GATATGGATTTAACGCCATTTAATTATATTTATCCTTGTGGTGATATTAACATCAAAATGACACAGGTTAAAGAATTTAA
CTCTTTTTTAACACTAAACGATATTCGTGTGATTTTAATTAAAAAGCTATCTCAGTTACTAAATGTTTCAATCATTGAAA
AATTTTCTATTAAATGA

Upstream 100 bases:

>100_bases
TTTTTCTGCATTACTACATTAATAATTTATCAAAATTAGTATTTTCGTTAAGTGTAGATTAAATATAGTATCGATCAAAT
ATAAAATTGGATTTTTCTCA

Downstream 100 bases:

>100_bases
AAAATACAATTTTTAGAAAAAAATTTTATTCTTATTAAGAATATAACATTGTATCAAATAATTACCTGTTAAGTTAATAA
TTACTATGTAAATTAAAATT

Product: lipoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MNNRIILFQNFGLKHWIDMFNKMHAFTEVRNINTYDEIWFLEHYPIFTQGLLQKINTITYNNDILHDIPIVSTDRGGQIT
YHGPGQQILYFLIDLKRRKITIRDLINIMQNLIIETLNYFSIKSHIKKNSPGVYVNNKKISSLGLRVKKGFTLHGLSLNV
DMDLTPFNYIYPCGDINIKMTQVKEFNSFLTLNDIRVILIKKLSQLLNVSIIEKFSIK

Sequences:

>Translated_218_residues
MNNRIILFQNFGLKHWIDMFNKMHAFTEVRNINTYDEIWFLEHYPIFTQGLLQKINTITYNNDILHDIPIVSTDRGGQIT
YHGPGQQILYFLIDLKRRKITIRDLINIMQNLIIETLNYFSIKSHIKKNSPGVYVNNKKISSLGLRVKKGFTLHGLSLNV
DMDLTPFNYIYPCGDINIKMTQVKEFNSFLTLNDIRVILIKKLSQLLNVSIIEKFSIK
>Mature_218_residues
MNNRIILFQNFGLKHWIDMFNKMHAFTEVRNINTYDEIWFLEHYPIFTQGLLQKINTITYNNDILHDIPIVSTDRGGQIT
YHGPGQQILYFLIDLKRRKITIRDLINIMQNLIIETLNYFSIKSHIKKNSPGVYVNNKKISSLGLRVKKGFTLHGLSLNV
DMDLTPFNYIYPCGDINIKMTQVKEFNSFLTLNDIRVILIKKLSQLLNVSIIEKFSIK

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=175, Percent_Identity=25.7142857142857, Blast_Score=74, Evalue=7e-14,
Organism=Escherichia coli, GI87081767, Length=203, Percent_Identity=44.3349753694581, Blast_Score=206, Evalue=9e-55,
Organism=Saccharomyces cerevisiae, GI6323268, Length=161, Percent_Identity=29.1925465838509, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355483, Length=175, Percent_Identity=29.1428571428571, Blast_Score=88, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_BUCAP (Q8K9Q3)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660605.2
- ProteinModelPortal:   Q8K9Q3
- EnsemblBacteria:   EBBUCT00000000131
- GeneID:   1005460
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg258
- GeneTree:   EBGT00050000008068
- HOGENOM:   HBG716160
- OMA:   THHLPGQ
- ProtClustDB:   CLSK2459967
- BioCyc:   BAPH198804:BUSG258-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 25552; Mature: 25552

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRIILFQNFGLKHWIDMFNKMHAFTEVRNINTYDEIWFLEHYPIFTQGLLQKINTITY
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCHHHHHHHHHHHEEEE
NNDILHDIPIVSTDRGGQITYHGPGQQILYFLIDLKRRKITIRDLINIMQNLIIETLNYF
CCCCEECCCEEECCCCCEEEEECCHHHHHHHEEEHHCCEEEHHHHHHHHHHHHHHHHHHH
SIKSHIKKNSPGVYVNNKKISSLGLRVKKGFTLHGLSLNVDMDLTPFNYIYPCGDINIKM
HHHHHHCCCCCCEEEECCEEHHCCEEEECCCEEEEEEEEEEECCCCCCEEEECCCCEEEE
TQVKEFNSFLTLNDIRVILIKKLSQLLNVSIIEKFSIK
EEEHHCCCEEEECHHHHHHHHHHHHHHCHHHHHHCCCC
>Mature Secondary Structure
MNNRIILFQNFGLKHWIDMFNKMHAFTEVRNINTYDEIWFLEHYPIFTQGLLQKINTITY
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCHHHHHHHHHHHEEEE
NNDILHDIPIVSTDRGGQITYHGPGQQILYFLIDLKRRKITIRDLINIMQNLIIETLNYF
CCCCEECCCEEECCCCCEEEEECCHHHHHHHEEEHHCCEEEHHHHHHHHHHHHHHHHHHH
SIKSHIKKNSPGVYVNNKKISSLGLRVKKGFTLHGLSLNVDMDLTPFNYIYPCGDINIKM
HHHHHHCCCCCCEEEECCEEHHCCEEEECCCEEEEEEEEEEECCCCCCEEEECCCCEEEE
TQVKEFNSFLTLNDIRVILIKKLSQLLNVSIIEKFSIK
EEEHHCCCEEEECHHHHHHHHHHHHHHCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438