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The map label for this gene is pyrF

Identifier: 21672540

GI number: 21672540

Start: 296820

End: 297530

Strand: Direct

Name: pyrF

Synonym: BUsg260

Alternate gene names: 21672540

Gene position: 296820-297530 (Clockwise)

Preceding gene: 21672539

Following gene: 21672541

Centisome position: 46.27

GC content: 26.16

Gene sequence:

>711_bases
GTGTTATACACTAACAATTACAATATTCCAAAAATTATTATTGCTTTAGATTTTTACAATAAGAAAGAAGCTATGACATT
AGTAGATCTTCTTGATCCTTCTGTTTTTTATTTAAAAATTGGTAAGGAAATGTTTACAATTTTGGGTTTTAAATTTGTAA
AAGAACTGCATAAGTTAGGCTTTAATGTATTTCTTGATTTAAAATTTCATGATATTCCAAATACTGTATTTAATGCTACA
AAAGCAGCTGCAGATTTAGGAATATGGATGTTAAGTGTTCATGCATCTGGTGGTAAAAATATGCTTCTTTCAGCAAAGAA
AGCGTTAAAATCTTTTAAAAAACCTCCTTTATTAATTGCTGTCACTATGTTGACTAGTTTAAAAGAAAAAGATTTAAAAG
AAATTGGAATTAAAATATCATTAAAGGACTATATTTTAATATTGTCAAAGTTATCTAATGATTGTGGTTTAGATGGAATT
GTCTGTCCAGGAAACCAAGCGAAAAAAATAAAATCATTATATGGAGATAAATATAAAATTATCACTCCAGGTATTAGATT
GTCCAGTGATTCTTCATTTGATCAAAAGCATATTATTACTCCAAAAGAAGCAAAAGAATTTCAAATAGATTACATAGTTA
TAGGACGTTCTATTACCACTTCAAAAAACCCAATTAAAAAATTAAATCTTATAATAGAATCTATGCGATAA

Upstream 100 bases:

>100_bases
ATTTAATTTTTTTTATTTTAAATAAATGTTTTAAATATTATTTAATTAGCATCTTTATATATTGAAAATATATAAAACTA
TTTCTAATAGAGGTTAAATA

Downstream 100 bases:

>100_bases
TATTTTTAGATAAATAAATATTTACATAAAATAATTTTGTAATAATATTTTAAGATATATCTTTCTAAGATAAATTGTTA
ATTATTTTTTTAAATAATTA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MLYTNNYNIPKIIIALDFYNKKEAMTLVDLLDPSVFYLKIGKEMFTILGFKFVKELHKLGFNVFLDLKFHDIPNTVFNAT
KAAADLGIWMLSVHASGGKNMLLSAKKALKSFKKPPLLIAVTMLTSLKEKDLKEIGIKISLKDYILILSKLSNDCGLDGI
VCPGNQAKKIKSLYGDKYKIITPGIRLSSDSSFDQKHIITPKEAKEFQIDYIVIGRSITTSKNPIKKLNLIIESMR

Sequences:

>Translated_236_residues
MLYTNNYNIPKIIIALDFYNKKEAMTLVDLLDPSVFYLKIGKEMFTILGFKFVKELHKLGFNVFLDLKFHDIPNTVFNAT
KAAADLGIWMLSVHASGGKNMLLSAKKALKSFKKPPLLIAVTMLTSLKEKDLKEIGIKISLKDYILILSKLSNDCGLDGI
VCPGNQAKKIKSLYGDKYKIITPGIRLSSDSSFDQKHIITPKEAKEFQIDYIVIGRSITTSKNPIKKLNLIIESMR
>Mature_236_residues
MLYTNNYNIPKIIIALDFYNKKEAMTLVDLLDPSVFYLKIGKEMFTILGFKFVKELHKLGFNVFLDLKFHDIPNTVFNAT
KAAADLGIWMLSVHASGGKNMLLSAKKALKSFKKPPLLIAVTMLTSLKEKDLKEIGIKISLKDYILILSKLSNDCGLDGI
VCPGNQAKKIKSLYGDKYKIITPGIRLSSDSSFDQKHIITPKEAKEFQIDYIVIGRSITTSKNPIKKLNLIIESMR

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=226, Percent_Identity=48.2300884955752, Blast_Score=238, Evalue=3e-64,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_BUCAP (Q8K9Q1)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660607.1
- ProteinModelPortal:   Q8K9Q1
- SMR:   Q8K9Q1
- EnsemblBacteria:   EBBUCT00000000467
- GeneID:   1005462
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg260
- GeneTree:   EBGT00050000008064
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   CLSK315846
- BioCyc:   BAPH198804:BUSG260-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 26562; Mature: 26562

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 68-68 BINDING 17-17 BINDING 39-39 BINDING 125-125 BINDING 186-186 BINDING 195-195 BINDING 215-215 BINDING 216-216

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYTNNYNIPKIIIALDFYNKKEAMTLVDLLDPSVFYLKIGKEMFTILGFKFVKELHKLG
CEECCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHCC
FNVFLDLKFHDIPNTVFNATKAAADLGIWMLSVHASGGKNMLLSAKKALKSFKKPPLLIA
CEEEEEEEEECCCHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEH
VTMLTSLKEKDLKEIGIKISLKDYILILSKLSNDCGLDGIVCPGNQAKKIKSLYGDKYKI
HHHHHHHHHCCHHHHCEEEEHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHCCCEEE
ITPGIRLSSDSSFDQKHIITPKEAKEFQIDYIVIGRSITTSKNPIKKLNLIIESMR
EECCEEECCCCCCCCCCCCCCCCCCCEEEEEEEEECEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLYTNNYNIPKIIIALDFYNKKEAMTLVDLLDPSVFYLKIGKEMFTILGFKFVKELHKLG
CEECCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHCC
FNVFLDLKFHDIPNTVFNATKAAADLGIWMLSVHASGGKNMLLSAKKALKSFKKPPLLIA
CEEEEEEEEECCCHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEH
VTMLTSLKEKDLKEIGIKISLKDYILILSKLSNDCGLDGIVCPGNQAKKIKSLYGDKYKI
HHHHHHHHHCCHHHHCEEEEHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHCCCEEE
ITPGIRLSSDSSFDQKHIITPKEAKEFQIDYIVIGRSITTSKNPIKKLNLIIESMR
EECCEEECCCCCCCCCCCCCCCCCCCEEEEEEEEECEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438