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The map label for this gene is grpE1

Identifier: 21672524

GI number: 21672524

Start: 280779

End: 281387

Strand: Direct

Name: grpE1

Synonym: BUsg243

Alternate gene names: 21672524

Gene position: 280779-281387 (Clockwise)

Preceding gene: 21672523

Following gene: 21672526

Centisome position: 43.77

GC content: 19.7

Gene sequence:

>609_bases
ATGAAAAATAAAAAAGAAGAAAAAATTAATAATTTAAAAAAAGAAGAAAAAATTAATAATTTAAAAAAAGAAGAAAAAAT
TAATAATTTAAAAAAAGAAGAAAAAGAAAAAAATGATGACGTTCAAAACAAAATAATAGATTTATTAGAAATAAAGTTAA
AGAAATCGCAAGAAAAAATTATTAATATGCAATTAAAGAATCATGAAGAAATTTTAAAATTAAATTATCGTTTAAATAGT
GATATAGAAAAATCTAGAAAGTTTTCTTTAGAAAAAGTAATAATTGAATTTCTTCCGATTATAGACAATATCGAACGTGC
TCTAAGTGTAATTAAAGATAAAAAAGAAGCATTTTATTTAGAAATTATTAATAAAATGAATTTTATTTTTTCCTTATTAG
AAGAAATTTTAAGTGAATTTAATGTGTCAAAAATTAATGAAAAAAACATTTCATTTGATCCAGAAATTCATCAAGCCATG
TCTATTAATTATAATGATGAAATAGAAGATAATCATGTAGTAGATGTTATGCAATCTGGTTATATGCTTCATAAAGCTCG
TTTATTGCGCCCTGCTATGGTTATTGTTTCAAAAAGAAAAAATAATTAA

Upstream 100 bases:

>100_bases
ATAATAATACTAATTTCAAAAATTAAAAAATTGTTTTTTATAAAAAATACTATAAAATTACATCAATAAATATGTATTAA
ATTGTAAAAAAGGTACATTT

Downstream 100 bases:

>100_bases
TTTTTTATTTTTTTAAACAAATATTTGATCTTTTTCTTCTTCGTTCTTTGGGATCGATTATTAAATTCCTATAAATTTCA
ATTCGATCTCCATTTTTTAC

Product: hypothetical protein

Products: NA

Alternate protein names: HSP-70 cofactor 1

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MKNKKEEKINNLKKEEKINNLKKEEKINNLKKEEKEKNDDVQNKIIDLLEIKLKKSQEKIINMQLKNHEEILKLNYRLNS
DIEKSRKFSLEKVIIEFLPIIDNIERALSVIKDKKEAFYLEIINKMNFIFSLLEEILSEFNVSKINEKNISFDPEIHQAM
SINYNDEIEDNHVVDVMQSGYMLHKARLLRPAMVIVSKRKNN

Sequences:

>Translated_202_residues
MKNKKEEKINNLKKEEKINNLKKEEKINNLKKEEKEKNDDVQNKIIDLLEIKLKKSQEKIINMQLKNHEEILKLNYRLNS
DIEKSRKFSLEKVIIEFLPIIDNIERALSVIKDKKEAFYLEIINKMNFIFSLLEEILSEFNVSKINEKNISFDPEIHQAM
SINYNDEIEDNHVVDVMQSGYMLHKARLLRPAMVIVSKRKNN
>Mature_202_residues
MKNKKEEKINNLKKEEKINNLKKEEKINNLKKEEKEKNDDVQNKIIDLLEIKLKKSQEKIINMQLKNHEEILKLNYRLNS
DIEKSRKFSLEKVIIEFLPIIDNIERALSVIKDKKEAFYLEIINKMNFIFSLLEEILSEFNVSKINEKNISFDPEIHQAM
SINYNDEIEDNHVVDVMQSGYMLHKARLLRPAMVIVSKRKNN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=198, Percent_Identity=31.8181818181818, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE1_BUCAP (Q8K9R7)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660591.1
- ProteinModelPortal:   Q8K9R7
- EnsemblBacteria:   EBBUCT00000000215
- GeneID:   1005444
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg243
- GeneTree:   EBGT00050000007962
- HOGENOM:   HBG732630
- OMA:   ERTSTEK
- ProtClustDB:   CLSK315757
- BioCyc:   BAPH198804:BUSG243-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 24068; Mature: 24068

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKKEEKINNLKKEEKINNLKKEEKINNLKKEEKEKNDDVQNKIIDLLEIKLKKSQEKI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
INMQLKNHEEILKLNYRLNSDIEKSRKFSLEKVIIEFLPIIDNIERALSVIKDKKEAFYL
HHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIINKMNFIFSLLEEILSEFNVSKINEKNISFDPEIHQAMSINYNDEIEDNHVVDVMQSG
HHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHCC
YMLHKARLLRPAMVIVSKRKNN
HHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNKKEEKINNLKKEEKINNLKKEEKINNLKKEEKEKNDDVQNKIIDLLEIKLKKSQEKI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
INMQLKNHEEILKLNYRLNSDIEKSRKFSLEKVIIEFLPIIDNIERALSVIKDKKEAFYL
HHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIINKMNFIFSLLEEILSEFNVSKINEKNISFDPEIHQAMSINYNDEIEDNHVVDVMQSG
HHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHCC
YMLHKARLLRPAMVIVSKRKNN
HHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438