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The map label for this gene is smpB

Identifier: 21672526

GI number: 21672526

Start: 281802

End: 282293

Strand: Direct

Name: smpB

Synonym: BUsg245

Alternate gene names: 21672526

Gene position: 281802-282293 (Clockwise)

Preceding gene: 21672524

Following gene: 21672527

Centisome position: 43.93

GC content: 22.76

Gene sequence:

>492_bases
ATGTCTTATAAAAAAAAACGTTGTTTAAAATCATCTATCATTTGTAAAAATAAAAAAGCGTACTATAACTATTTTATAGA
GGAAGTATTTCAATCAGGTCTTGTATTAATGGGATGGGAAGTAAAATCAATTCGATTAGGACAAGTAAATATTATTGAAA
GTTACATTAATAATGATAATTTGAATGAAATGTATCTTTATAATTCTATTGTTCAACCTTTACATACATCTTCGAATTAT
GTATCGTGTGATTCTTCTAGAAAAAGAAAATTACTTTTGCATAAAAATGAAATTCAATATTTATGTAATAAAAAAAATAA
AATAGGTTCTACATTAGTTGCATTATCTTTGTTTTGGGAAAAATCTTGGTGTAAATTAAATTTTGGATTAGCTAAGGGAA
AAACTAAAATAGATAAAAGAGCGAGTGAAAAACAAAACGAATGGAAAAAAGAAAAATTAAAAATTTTAAAGAAAGTTAAA
TTTCAGAATTAA

Upstream 100 bases:

>100_bases
ATAAACTCTTTCTAACAAACTTTTAAAAATAAATCAATATATATAAGTAAAATTTTTTAAAAAATATTTAAATATAAGTT
TTTTCGGATTACATCTAATT

Downstream 100 bases:

>100_bases
AAATATTTTATGAAAAGTAATCGCGATAGCTATTGGATGAAAATTGCTTTAAAATATGCATATTATGCAGAAGAAAATGG
AGAGGTACCTATAGGTGCAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MSYKKKRCLKSSIICKNKKAYYNYFIEEVFQSGLVLMGWEVKSIRLGQVNIIESYINNDNLNEMYLYNSIVQPLHTSSNY
VSCDSSRKRKLLLHKNEIQYLCNKKNKIGSTLVALSLFWEKSWCKLNFGLAKGKTKIDKRASEKQNEWKKEKLKILKKVK
FQN

Sequences:

>Translated_163_residues
MSYKKKRCLKSSIICKNKKAYYNYFIEEVFQSGLVLMGWEVKSIRLGQVNIIESYINNDNLNEMYLYNSIVQPLHTSSNY
VSCDSSRKRKLLLHKNEIQYLCNKKNKIGSTLVALSLFWEKSWCKLNFGLAKGKTKIDKRASEKQNEWKKEKLKILKKVK
FQN
>Mature_162_residues
SYKKKRCLKSSIICKNKKAYYNYFIEEVFQSGLVLMGWEVKSIRLGQVNIIESYINNDNLNEMYLYNSIVQPLHTSSNYV
SCDSSRKRKLLLHKNEIQYLCNKKNKIGSTLVALSLFWEKSWCKLNFGLAKGKTKIDKRASEKQNEWKKEKLKILKKVKF
QN

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=157, Percent_Identity=44.5859872611465, Blast_Score=147, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_BUCAP (Q8K9R5)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660593.1
- ProteinModelPortal:   Q8K9R5
- SMR:   Q8K9R5
- EnsemblBacteria:   EBBUCT00000000134
- GeneID:   1005446
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg245
- GeneTree:   EBGT00050000007781
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   BAPH198804:BUSG245-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 19246; Mature: 19115

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYKKKRCLKSSIICKNKKAYYNYFIEEVFQSGLVLMGWEVKSIRLGQVNIIESYINNDN
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEEECEEECCHHHHHHHHHCCCC
LNEMYLYNSIVQPLHTSSNYVSCDSSRKRKLLLHKNEIQYLCNKKNKIGSTLVALSLFWE
CCHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHC
KSWCKLNFGLAKGKTKIDKRASEKQNEWKKEKLKILKKVKFQN
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYKKKRCLKSSIICKNKKAYYNYFIEEVFQSGLVLMGWEVKSIRLGQVNIIESYINNDN
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEEECEEECCHHHHHHHHHCCCC
LNEMYLYNSIVQPLHTSSNYVSCDSSRKRKLLLHKNEIQYLCNKKNKIGSTLVALSLFWE
CCHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHC
KSWCKLNFGLAKGKTKIDKRASEKQNEWKKEKLKILKKVKFQN
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438