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The map label for this gene is aceE

Identifier: 21672485

GI number: 21672485

Start: 224713

End: 227379

Strand: Direct

Name: aceE

Synonym: BUsg199

Alternate gene names: 21672485

Gene position: 224713-227379 (Clockwise)

Preceding gene: 21672484

Following gene: 21672486

Centisome position: 35.03

GC content: 29.66

Gene sequence:

>2667_bases
ATGTCAGAAAAGTTATATAATTATGACGTGGATCCAGTCGAAACTAATGATTGGGTGCAATCAATTGAATCTGTTATTCG
TGAAGAAGGTCTTGAAAGAGCTAAATTTTTAATTGAAAAGATCTTAAAAAAATCTAAAATAACTAGAGCAAATTTTTTTA
AATGTTTTTTTACAAGTGATTATATTAATACAATTAGTAGCGAAGAAGAAGTTGAATATCCAGGTGATTTAATTTTAGAA
AAGCGTATTCGTTCAGCTATTCGTTGGAACGCAATAATGATGGTTTTACGTGCTTCAAAAAAAGATTTAGAATTAGGTGG
ACATTTATCATCTTTTCAATCTTCTGCAACAATATATGAAGTATGTTTTAATCATTTTTTTCGTTCTAAAAATGATGAAG
ATGGAGGCGATTTAGTTTATTTTCAAGGTCATATTGCTCCAGGTATTTATGCTAGATCTTTTTTAGAAGGCCGTTTATCA
AAAAAACAAATTGATAATTTTAGACAAGAAGTTGATGGGAAGGGATTATCTTCTTATCCTCATCCTAAGTTAATGCCAAA
TTTTTGGCAATTTCCAACTGTTTCAATGGGATTAGGCCCACTGTGTGCTATTTACCAAGCAAAGTTTTTAAAATACTTGC
AGAATCGTGAATTAAAAAATACCTCTAAACAAACGGTTTATGCTTTTTTAGGAGATGGTGAAATGGATGAACCAGAATCT
AAAGGAGCAATTTCTATTGCTGTTCGCGAAAAATTAGATAATTTAATTTTTGTAATAAATTGTAACTTACAAAGATTAGA
TGGTCCAGTGGTAGGTAATGGAAAAATTGTGAACGAATTAGAAAGTTTTTTTTATGGTGCTGGATGGAAAGTAATAAAAG
TTATATGGGGAGGAAAATGGGATTCCTTATTGAAAAAAGATAAAACTGGAAAATTGATTCAACTAATGAATGAAACAATA
GACGGTGAATATCAAACATTAAAATCAAAAGATGGAGCATACGTTCGAAAATATTTTTTTGGTAAATATCAAGAAACACT
AGAGTTAGTGAAAAACATGACGGATGAAGAAATATGGAATTTAAACAGAGGAGGACATGATCCTAAAAAGATGTTTAATG
CACTTAAAAAAGCTAAAGAAATTAAAGATAAACCAACTGTGATTTTGGCACATACAGTGAAAGGATACGGTATGGGTGTT
ATTGCTGAAGGGAAAAATATAGCGCATCAAATAAAAAAAATTAATATTAATGGAATTATATATATTAGAGATCGTTTTAA
TATTCCTATATCAAATGAAGATATTAAAGAATTACCGTATGTTGTATTTGAAAAAAACTCAAAAGAATACTGCTATATGC
ATCAACAAAGAAAAAAACTAGGTGGTTACATTCCATTTCGTTTGTCTAAATTTACTAATGCACTAAACATTCCAGATTTA
ATAGATTTTAAATCTTTATTAAAAGAACAAAACAAAAAAATGTCAACAACTATTGCTTTTGTTCGTGTTTTAAATTTAAT
TTTAAAGAATCATTCCATTAAAAATTTGATAGTTCCGATTATTGCTGATGAGGCTCGTACATTTGGCATGGAAGGTTTAT
TTAGAATGATTGGAATTTATAGTTCTATTGGACAAAAATATGTTCCTCAAGATCGAGAACAATTAGCTTATTATAAAGAA
GAAAAAAAAGGACAAATACTGCAAGAAGGAATAAATGAATTAGGTGCTGCTTCATCTTGGTTAGCTGCTGCTACTTCTTA
TAGTACTAATGATTTTCCTATGATTCCCTTTTACATTTATTATTCAATATTTGGTTTTCAAAGAATAGGTGATCTTTTTT
GGGCTGCTGGAGATCAACAAGCAAGAGGTTTTCTCATTGGAGGAACTTCTGGAAGAACTACTTTAAACGGAGAAGGTTTA
CAGCATGAAGATGGTCATAGTCATATACAGTCATTAACGATTCCTAATTGTGTTTCTTATGATCCTGCATTTGCTTATGA
AGTTGCCGTTATTATACAGGATGGATTAAGAAGAATGTATGGCCCTCTCCAAGAGAATATATACTATTATATTACAACAA
TTAATGAGAATTATTATATGCCTGCTATGCCTCAAGGTGTGGAAAAAGGCATTTGTAAAGGTATTTATAAGTTAAAAACT
TTTTATGCCACTGAATTAAAAGTTCAATTAATGGGATCTGGAGCGATTTTACGTTGTATTTGTAAAGCTGGTGAGATTTT
GTCTAATGATTATTGTATAACTACAGATATTTATAGTGTAACTTCTTTTACAGAATTAGCTAGAAATGGTGAAGATTGTG
AAAGATGGAACATGTTACATCCGTATGAAAAGAAAAGAATTGCTTATATTAAAACTGTAATGAATTCCTCTCCAGCTGTA
GCAGCAACTGATTATATGAAGTTATTTGCTGAACAAATTCGTCATTATATTCCATCAAATGAATATCATGTATTGGGTAC
AGATGGTTTTGGACGTTCAGATAGTCGTGATAAATTGCGTGATCATTTTGAAGTTAGTGCTTATTATATTGTAGTAGCTG
CTTTAAACTTATTAGCTAAATTAAATAATATTAATAAAAAAGTAGTAGAAGAAGCAATTATTAAATTTAATATTAATGCA
GATAAAATTAATCCGCGTTTAGCTTAA

Upstream 100 bases:

>100_bases
TATTTAATTATATAAAATTAACTATTAATATTACAATAATAATATTTTAAGTCAAATATTCATTTTGTCATTAAATATAA
ATACATAAGGAATTTAGACC

Downstream 100 bases:

>100_bases
GAGGTGAAAAAAGTGCATATTGAAGTTAAAATGCCTGATATTGGTTTAGAAGAAGTTGAGGTAACAGAAATATTAGTAAA
AATTGGTGAAGAAATAAAAT

Product: pyruvate dehydrogenase subunit E1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 888; Mature: 887

Protein sequence:

>888_residues
MSEKLYNYDVDPVETNDWVQSIESVIREEGLERAKFLIEKILKKSKITRANFFKCFFTSDYINTISSEEEVEYPGDLILE
KRIRSAIRWNAIMMVLRASKKDLELGGHLSSFQSSATIYEVCFNHFFRSKNDEDGGDLVYFQGHIAPGIYARSFLEGRLS
KKQIDNFRQEVDGKGLSSYPHPKLMPNFWQFPTVSMGLGPLCAIYQAKFLKYLQNRELKNTSKQTVYAFLGDGEMDEPES
KGAISIAVREKLDNLIFVINCNLQRLDGPVVGNGKIVNELESFFYGAGWKVIKVIWGGKWDSLLKKDKTGKLIQLMNETI
DGEYQTLKSKDGAYVRKYFFGKYQETLELVKNMTDEEIWNLNRGGHDPKKMFNALKKAKEIKDKPTVILAHTVKGYGMGV
IAEGKNIAHQIKKININGIIYIRDRFNIPISNEDIKELPYVVFEKNSKEYCYMHQQRKKLGGYIPFRLSKFTNALNIPDL
IDFKSLLKEQNKKMSTTIAFVRVLNLILKNHSIKNLIVPIIADEARTFGMEGLFRMIGIYSSIGQKYVPQDREQLAYYKE
EKKGQILQEGINELGAASSWLAAATSYSTNDFPMIPFYIYYSIFGFQRIGDLFWAAGDQQARGFLIGGTSGRTTLNGEGL
QHEDGHSHIQSLTIPNCVSYDPAFAYEVAVIIQDGLRRMYGPLQENIYYYITTINENYYMPAMPQGVEKGICKGIYKLKT
FYATELKVQLMGSGAILRCICKAGEILSNDYCITTDIYSVTSFTELARNGEDCERWNMLHPYEKKRIAYIKTVMNSSPAV
AATDYMKLFAEQIRHYIPSNEYHVLGTDGFGRSDSRDKLRDHFEVSAYYIVVAALNLLAKLNNINKKVVEEAIIKFNINA
DKINPRLA

Sequences:

>Translated_888_residues
MSEKLYNYDVDPVETNDWVQSIESVIREEGLERAKFLIEKILKKSKITRANFFKCFFTSDYINTISSEEEVEYPGDLILE
KRIRSAIRWNAIMMVLRASKKDLELGGHLSSFQSSATIYEVCFNHFFRSKNDEDGGDLVYFQGHIAPGIYARSFLEGRLS
KKQIDNFRQEVDGKGLSSYPHPKLMPNFWQFPTVSMGLGPLCAIYQAKFLKYLQNRELKNTSKQTVYAFLGDGEMDEPES
KGAISIAVREKLDNLIFVINCNLQRLDGPVVGNGKIVNELESFFYGAGWKVIKVIWGGKWDSLLKKDKTGKLIQLMNETI
DGEYQTLKSKDGAYVRKYFFGKYQETLELVKNMTDEEIWNLNRGGHDPKKMFNALKKAKEIKDKPTVILAHTVKGYGMGV
IAEGKNIAHQIKKININGIIYIRDRFNIPISNEDIKELPYVVFEKNSKEYCYMHQQRKKLGGYIPFRLSKFTNALNIPDL
IDFKSLLKEQNKKMSTTIAFVRVLNLILKNHSIKNLIVPIIADEARTFGMEGLFRMIGIYSSIGQKYVPQDREQLAYYKE
EKKGQILQEGINELGAASSWLAAATSYSTNDFPMIPFYIYYSIFGFQRIGDLFWAAGDQQARGFLIGGTSGRTTLNGEGL
QHEDGHSHIQSLTIPNCVSYDPAFAYEVAVIIQDGLRRMYGPLQENIYYYITTINENYYMPAMPQGVEKGICKGIYKLKT
FYATELKVQLMGSGAILRCICKAGEILSNDYCITTDIYSVTSFTELARNGEDCERWNMLHPYEKKRIAYIKTVMNSSPAV
AATDYMKLFAEQIRHYIPSNEYHVLGTDGFGRSDSRDKLRDHFEVSAYYIVVAALNLLAKLNNINKKVVEEAIIKFNINA
DKINPRLA
>Mature_887_residues
SEKLYNYDVDPVETNDWVQSIESVIREEGLERAKFLIEKILKKSKITRANFFKCFFTSDYINTISSEEEVEYPGDLILEK
RIRSAIRWNAIMMVLRASKKDLELGGHLSSFQSSATIYEVCFNHFFRSKNDEDGGDLVYFQGHIAPGIYARSFLEGRLSK
KQIDNFRQEVDGKGLSSYPHPKLMPNFWQFPTVSMGLGPLCAIYQAKFLKYLQNRELKNTSKQTVYAFLGDGEMDEPESK
GAISIAVREKLDNLIFVINCNLQRLDGPVVGNGKIVNELESFFYGAGWKVIKVIWGGKWDSLLKKDKTGKLIQLMNETID
GEYQTLKSKDGAYVRKYFFGKYQETLELVKNMTDEEIWNLNRGGHDPKKMFNALKKAKEIKDKPTVILAHTVKGYGMGVI
AEGKNIAHQIKKININGIIYIRDRFNIPISNEDIKELPYVVFEKNSKEYCYMHQQRKKLGGYIPFRLSKFTNALNIPDLI
DFKSLLKEQNKKMSTTIAFVRVLNLILKNHSIKNLIVPIIADEARTFGMEGLFRMIGIYSSIGQKYVPQDREQLAYYKEE
KKGQILQEGINELGAASSWLAAATSYSTNDFPMIPFYIYYSIFGFQRIGDLFWAAGDQQARGFLIGGTSGRTTLNGEGLQ
HEDGHSHIQSLTIPNCVSYDPAFAYEVAVIIQDGLRRMYGPLQENIYYYITTINENYYMPAMPQGVEKGICKGIYKLKTF
YATELKVQLMGSGAILRCICKAGEILSNDYCITTDIYSVTSFTELARNGEDCERWNMLHPYEKKRIAYIKTVMNSSPAVA
ATDYMKLFAEQIRHYIPSNEYHVLGTDGFGRSDSRDKLRDHFEVSAYYIVVAALNLLAKLNNINKKVVEEAIIKFNINAD
KINPRLA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG2609

COG function: function code C; Pyruvate dehydrogenase complex, dehydrogenase (E1) component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786304, Length=888, Percent_Identity=73.536036036036, Blast_Score=1410, Evalue=0.0,

Paralogues:

None

Copy number: 1140 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 400 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ODP1_BUCAP (Q8K9T9)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660552.1
- ProteinModelPortal:   Q8K9T9
- SMR:   Q8K9T9
- EnsemblBacteria:   EBBUCT00000000201
- GeneID:   1005396
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg199
- GeneTree:   EBGT00050000007777
- HOGENOM:   HBG289271
- OMA:   EVDRHFV
- ProtClustDB:   PRK09405
- BioCyc:   BAPH198804:BUSG199-MONOMER
- GO:   GO:0006096
- InterPro:   IPR004660
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005474
- Gene3D:   G3DSA:3.40.50.920
- PIRSF:   PIRSF000156
- TIGRFAMs:   TIGR00759

Pfam domain/function: PF00456 Transketolase_N; SSF52922 Transketo_C_like

EC number: =1.2.4.1

Molecular weight: Translated: 101459; Mature: 101328

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLYNYDVDPVETNDWVQSIESVIREEGLERAKFLIEKILKKSKITRANFFKCFFTSD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEEHH
YINTISSEEEVEYPGDLILEKRIRSAIRWNAIMMVLRASKKDLELGGHLSSFQSSATIYE
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHH
VCFNHFFRSKNDEDGGDLVYFQGHIAPGIYARSFLEGRLSKKQIDNFRQEVDGKGLSSYP
HHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HPKLMPNFWQFPTVSMGLGPLCAIYQAKFLKYLQNRELKNTSKQTVYAFLGDGEMDEPES
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCC
KGAISIAVREKLDNLIFVINCNLQRLDGPVVGNGKIVNELESFFYGAGWKVIKVIWGGKW
CCCEEEEEHHHHCCEEEEEECCCEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCH
DSLLKKDKTGKLIQLMNETIDGEYQTLKSKDGAYVRKYFFGKYQETLELVKNMTDEEIWN
HHHHHCCCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHC
LNRGGHDPKKMFNALKKAKEIKDKPTVILAHTVKGYGMGVIAEGKNIAHQIKKININGII
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCHHHHHHHHEEECCEEE
YIRDRFNIPISNEDIKELPYVVFEKNSKEYCYMHQQRKKLGGYIPFRLSKFTNALNIPDL
EEEECCCCCCCCCCHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHH
IDFKSLLKEQNKKMSTTIAFVRVLNLILKNHSIKNLIVPIIADEARTFGMEGLFRMIGIY
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEECHHHHHCHHHHHHHHHHH
SSIGQKYVPQDREQLAYYKEEKKGQILQEGINELGAASSWLAAATSYSTNDFPMIPFYIY
HHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHH
YSIFGFQRIGDLFWAAGDQQARGFLIGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCVSY
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHCCCCCCCCC
DPAFAYEVAVIIQDGLRRMYGPLQENIYYYITTINENYYMPAMPQGVEKGICKGIYKLKT
CCHHHHHHHHHHHHHHHHHHCHHHCCEEEEEEEECCCEECCCCCCHHHHHHHHHHHHHHH
FYATELKVQLMGSGAILRCICKAGEILSNDYCITTDIYSVTSFTELARNGEDCERWNMLH
HHEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCHHHCCCCC
PYEKKRIAYIKTVMNSSPAVAATDYMKLFAEQIRHYIPSNEYHVLGTDGFGRSDSRDKLR
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHH
DHFEVSAYYIVVAALNLLAKLNNINKKVVEEAIIKFNINADKINPRLA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCHHCCCCCCC
>Mature Secondary Structure 
SEKLYNYDVDPVETNDWVQSIESVIREEGLERAKFLIEKILKKSKITRANFFKCFFTSD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEEHH
YINTISSEEEVEYPGDLILEKRIRSAIRWNAIMMVLRASKKDLELGGHLSSFQSSATIYE
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHH
VCFNHFFRSKNDEDGGDLVYFQGHIAPGIYARSFLEGRLSKKQIDNFRQEVDGKGLSSYP
HHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HPKLMPNFWQFPTVSMGLGPLCAIYQAKFLKYLQNRELKNTSKQTVYAFLGDGEMDEPES
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCC
KGAISIAVREKLDNLIFVINCNLQRLDGPVVGNGKIVNELESFFYGAGWKVIKVIWGGKW
CCCEEEEEHHHHCCEEEEEECCCEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCH
DSLLKKDKTGKLIQLMNETIDGEYQTLKSKDGAYVRKYFFGKYQETLELVKNMTDEEIWN
HHHHHCCCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHC
LNRGGHDPKKMFNALKKAKEIKDKPTVILAHTVKGYGMGVIAEGKNIAHQIKKININGII
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCHHHHHHHHEEECCEEE
YIRDRFNIPISNEDIKELPYVVFEKNSKEYCYMHQQRKKLGGYIPFRLSKFTNALNIPDL
EEEECCCCCCCCCCHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHH
IDFKSLLKEQNKKMSTTIAFVRVLNLILKNHSIKNLIVPIIADEARTFGMEGLFRMIGIY
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEECHHHHHCHHHHHHHHHHH
SSIGQKYVPQDREQLAYYKEEKKGQILQEGINELGAASSWLAAATSYSTNDFPMIPFYIY
HHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHH
YSIFGFQRIGDLFWAAGDQQARGFLIGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCVSY
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHCCCCCCCCC
DPAFAYEVAVIIQDGLRRMYGPLQENIYYYITTINENYYMPAMPQGVEKGICKGIYKLKT
CCHHHHHHHHHHHHHHHHHHCHHHCCEEEEEEEECCCEECCCCCCHHHHHHHHHHHHHHH
FYATELKVQLMGSGAILRCICKAGEILSNDYCITTDIYSVTSFTELARNGEDCERWNMLH
HHEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCHHHCCCCC
PYEKKRIAYIKTVMNSSPAVAATDYMKLFAEQIRHYIPSNEYHVLGTDGFGRSDSRDKLR
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHH
DHFEVSAYYIVVAALNLLAKLNNINKKVVEEAIIKFNINADKINPRLA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438