Click here to switch to the map view.

The map label for this gene is aceF [H]

Identifier: 21672486

GI number: 21672486

Start: 227383

End: 228618

Strand: Direct

Name: aceF [H]

Synonym: BUsg200

Alternate gene names: 21672486

Gene position: 227383-228618 (Clockwise)

Preceding gene: 21672485

Following gene: 21672487

Centisome position: 35.45

GC content: 24.68

Gene sequence:

>1236_bases
GTGAAAAAAGTGCATATTGAAGTTAAAATGCCTGATATTGGTTTAGAAGAAGTTGAGGTAACAGAAATATTAGTAAAAAT
TGGTGAAGAAATAAAATTAGATCAAGGATTAATAACTGTAGAAGGAGATAAAGCTTCTATGGAAATACCCTCACCAATAT
CTGGAATTGTAAAAGATATTACTATAAAAATTGGTGAGAAAGTAAAAACTTCTTCTATAATTATGATTTTTAAAGTTAAT
AACTTGAATTCTATTAAAAATGAAAAAGATTTAAATTATATAAAAACAGAAAAAAAATTAAATGAAAACTTTTTAGAAGA
AAAAAAAGATATTAAGAAAATTGTTTTAGTTCATGCAACACCAGTTGTAAGACGTCTAGCTCGTCATTTAAACGTTGATT
TAAAAAATATTACACCTTCTGGTCCTAAAAATCGTATCTTAAAAGAAGATATTGAATTATATATACGAAATAATACTTCA
AATAAAAGTTCTTTTAATATAGAAAAAAATAATACTACAAATTTTCATAAAGATTTGTTTAATGAAATACCTATTACAAA
TATTCAACAAATTATTGGTAAGAATTTACATCAAAATTGGGTCAACATCCCCCACGTTACTCAATTTGATGAAGTTAACA
TAACTTTATTAGAAGAATTTCGTCATAAATATAATACTGAAAAAAAACAAAAAAATAATATGTGTTCTAAAATTACTATA
TTACCTTTCATTATAAAATCTGTCGCATATGGGTTATTAGAATTTCCTATTTTTAATAGTTCCCTATCTGTGAATAAAAA
AACAATTTTTTTAAAAAAATATGTTAATGTTGGTATTGCTGTTGATGTTCAAAATGCTTTATTTGTCCCTGTTTTAAAAA
ATGTTGATAAAAAAAATATAGCAAATTTATCTTCTGAATTAATATTTCTTTCAAAAAAAGCACATGAAAATAAATTAGAT
GCATCAGATATGAAAGATGGTTGTTTTACAATTTCTAATTTAGGTGGAATTGGAGGGAGTTGGTTTTCTCCCATTATTAA
TTCTCCTGAAGTTGCAATTCTTGGAGTTTCAAAAGCTTTAATAAAACCATTATGGAATGGAAAAGAATTTATCCCTTCTT
TAATGTTGCCATTATCTTTATCATATGATCATCGCGTTATTAATGGAGCAGATGCCGCTCGTTTTTTAACTTTTATTGGA
AAAATGTTATCTGATATACGTTTTTTAATTATGTGA

Upstream 100 bases:

>100_bases
ATTAGCTAAATTAAATAATATTAATAAAAAAGTAGTAGAAGAAGCAATTATTAAATTTAATATTAATGCAGATAAAATTA
ATCCGCGTTTAGCTTAAGAG

Downstream 100 bases:

>100_bases
TTTTTTTTCTAAAGAGGTTTATTAAAATGCATCAAGAAATTCAATCTGAAGTCGTAATTATAGGTTCAGGACCTGCAGGT
TATTCTGCTGCCTTTCGATG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MKKVHIEVKMPDIGLEEVEVTEILVKIGEEIKLDQGLITVEGDKASMEIPSPISGIVKDITIKIGEKVKTSSIIMIFKVN
NLNSIKNEKDLNYIKTEKKLNENFLEEKKDIKKIVLVHATPVVRRLARHLNVDLKNITPSGPKNRILKEDIELYIRNNTS
NKSSFNIEKNNTTNFHKDLFNEIPITNIQQIIGKNLHQNWVNIPHVTQFDEVNITLLEEFRHKYNTEKKQKNNMCSKITI
LPFIIKSVAYGLLEFPIFNSSLSVNKKTIFLKKYVNVGIAVDVQNALFVPVLKNVDKKNIANLSSELIFLSKKAHENKLD
ASDMKDGCFTISNLGGIGGSWFSPIINSPEVAILGVSKALIKPLWNGKEFIPSLMLPLSLSYDHRVINGADAARFLTFIG
KMLSDIRFLIM

Sequences:

>Translated_411_residues
MKKVHIEVKMPDIGLEEVEVTEILVKIGEEIKLDQGLITVEGDKASMEIPSPISGIVKDITIKIGEKVKTSSIIMIFKVN
NLNSIKNEKDLNYIKTEKKLNENFLEEKKDIKKIVLVHATPVVRRLARHLNVDLKNITPSGPKNRILKEDIELYIRNNTS
NKSSFNIEKNNTTNFHKDLFNEIPITNIQQIIGKNLHQNWVNIPHVTQFDEVNITLLEEFRHKYNTEKKQKNNMCSKITI
LPFIIKSVAYGLLEFPIFNSSLSVNKKTIFLKKYVNVGIAVDVQNALFVPVLKNVDKKNIANLSSELIFLSKKAHENKLD
ASDMKDGCFTISNLGGIGGSWFSPIINSPEVAILGVSKALIKPLWNGKEFIPSLMLPLSLSYDHRVINGADAARFLTFIG
KMLSDIRFLIM
>Mature_411_residues
MKKVHIEVKMPDIGLEEVEVTEILVKIGEEIKLDQGLITVEGDKASMEIPSPISGIVKDITIKIGEKVKTSSIIMIFKVN
NLNSIKNEKDLNYIKTEKKLNENFLEEKKDIKKIVLVHATPVVRRLARHLNVDLKNITPSGPKNRILKEDIELYIRNNTS
NKSSFNIEKNNTTNFHKDLFNEIPITNIQQIIGKNLHQNWVNIPHVTQFDEVNITLLEEFRHKYNTEKKQKNNMCSKITI
LPFIIKSVAYGLLEFPIFNSSLSVNKKTIFLKKYVNVGIAVDVQNALFVPVLKNVDKKNIANLSSELIFLSKKAHENKLD
ASDMKDGCFTISNLGGIGGSWFSPIINSPEVAILGVSKALIKPLWNGKEFIPSLMLPLSLSYDHRVINGADAARFLTFIG
KMLSDIRFLIM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=31.7016317016317, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI31711992, Length=292, Percent_Identity=31.8493150684932, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI19923748, Length=228, Percent_Identity=32.0175438596491, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI203098753, Length=455, Percent_Identity=22.4175824175824, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI203098816, Length=455, Percent_Identity=22.4175824175824, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1786305, Length=436, Percent_Identity=45.8715596330275, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1786946, Length=390, Percent_Identity=26.9230769230769, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=406, Percent_Identity=27.3399014778325, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=388, Percent_Identity=27.0618556701031, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560088, Length=430, Percent_Identity=26.046511627907, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=28.8461538461538, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=413, Percent_Identity=24.455205811138, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6324258, Length=298, Percent_Identity=25.503355704698, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859875, Length=400, Percent_Identity=29.25, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24582497, Length=294, Percent_Identity=29.2517006802721, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI20129315, Length=294, Percent_Identity=29.2517006802721, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24645909, Length=191, Percent_Identity=32.9842931937173, Blast_Score=108, Evalue=8e-24,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46515; Mature: 46515

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVHIEVKMPDIGLEEVEVTEILVKIGEEIKLDQGLITVEGDKASMEIPSPISGIVKDI
CCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEECCCEEEEECCCCCCCCCCHHHHHHHHH
TIKIGEKVKTSSIIMIFKVNNLNSIKNEKDLNYIKTEKKLNENFLEEKKDIKKIVLVHAT
HHEECCCEECCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECC
PVVRRLARHLNVDLKNITPSGPKNRILKEDIELYIRNNTSNKSSFNIEKNNTTNFHKDLF
HHHHHHHHHHCCEEECCCCCCCCCHHHHHCEEEEEECCCCCCCEEEEECCCCCHHHHHHH
NEIPITNIQQIIGKNLHQNWVNIPHVTQFDEVNITLLEEFRHKYNTEKKQKNNMCSKITI
HCCCHHHHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCHHHHHHH
LPFIIKSVAYGLLEFPIFNSSLSVNKKTIFLKKYVNVGIAVDVQNALFVPVLKNVDKKNI
HHHHHHHHHHHHHHCCEECCCCCCCCCEEEEEHHHCCCEEEEECCHHHHHHHHCCCHHHH
ANLSSELIFLSKKAHENKLDASDMKDGCFTISNLGGIGGSWFSPIINSPEVAILGVSKAL
HHHHHHEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCEEEEECHHHH
IKPLWNGKEFIPSLMLPLSLSYDHRVINGADAARFLTFIGKMLSDIRFLIM
HHHHCCCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKVHIEVKMPDIGLEEVEVTEILVKIGEEIKLDQGLITVEGDKASMEIPSPISGIVKDI
CCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEECCCEEEEECCCCCCCCCCHHHHHHHHH
TIKIGEKVKTSSIIMIFKVNNLNSIKNEKDLNYIKTEKKLNENFLEEKKDIKKIVLVHAT
HHEECCCEECCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECC
PVVRRLARHLNVDLKNITPSGPKNRILKEDIELYIRNNTSNKSSFNIEKNNTTNFHKDLF
HHHHHHHHHHCCEEECCCCCCCCCHHHHHCEEEEEECCCCCCCEEEEECCCCCHHHHHHH
NEIPITNIQQIIGKNLHQNWVNIPHVTQFDEVNITLLEEFRHKYNTEKKQKNNMCSKITI
HCCCHHHHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCHHHHHHH
LPFIIKSVAYGLLEFPIFNSSLSVNKKTIFLKKYVNVGIAVDVQNALFVPVLKNVDKKNI
HHHHHHHHHHHHHHCCEECCCCCCCCCEEEEEHHHCCCEEEEECCHHHHHHHHCCCHHHH
ANLSSELIFLSKKAHENKLDASDMKDGCFTISNLGGIGGSWFSPIINSPEVAILGVSKAL
HHHHHHEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCEEEEECHHHH
IKPLWNGKEFIPSLMLPLSLSYDHRVINGADAARFLTFIGKMLSDIRFLIM
HHHHCCCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]