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The map label for this gene is tyrS

Identifier: 21672404

GI number: 21672404

Start: 126368

End: 127636

Strand: Direct

Name: tyrS

Synonym: BUsg113

Alternate gene names: 21672404

Gene position: 126368-127636 (Clockwise)

Preceding gene: 21672403

Following gene: 21672406

Centisome position: 19.7

GC content: 24.74

Gene sequence:

>1269_bases
ATGGATTTAATTAATGAATTACATCAAAGAAATTTAATATTTCATATTACAAATGAAAATAAATTAAAAGAACTTATTAA
AAATAAATATATTTCACTTTATTGTGGTTTTGATCCTACAGAAGATAGTTTACATGTGGGTCATCTATTACCTTTAATCA
CTCTTAAAAGATTTCAAGTTTTAGGTCATAAACCTATAGTGTTAATAGGAGGCGCTACAAGTCTTATTGGAGACCCTAGT
TTTAAAACAAAAGAAAGATTGCTAAAATCAGATAGTTTTGTGGAAAAATGCACACTAAAAATAAGTCAACAAATTTCTTT
TTTCTTAGATTTTAATTCTAATTTTAATAGTGCAATTATATTAAATAATAATTTGTGGTTTAAAAAGATTAATATTCTTC
AATTTTTACGTGATGTTGGCAAATATTTTTCAGTTAACGCTTTAATTAATAGAGAAGCTGTTAAAAAACGAATTAAAAGG
TCAGATCAAGGTATTTCTTTTACAGAATTTTCATATAATTTATTACAAGCATACGATTTTTTTATACTAAATCAAAAAAA
TCAAGTATCACTCCAAATTGGGGGCTCTGATCAATGGGGAAATATTTCTTCAGGTATGCATTTAATTCATCGAATATCTA
AAAAAGAAGCTTATGGTTTAACTCTTCCATTACTTATGCAATCTAATGGTATTAAATTTGGAAAAACAGAATCAGGAACT
GTGTGGCTAGATCCTCAAAAAACTACTCCTTATAGATTTTATCAGTTTTGGATGAATATAGAAGATTCTAATGTTTACCG
TTTTTTAAAATTATTTACTTTTATAGATATTCATGAAATAAATGAACGGGAAAAAAATAGCTATAAAAAAAATCAAATTG
TTTCTGATAAGGTTTTATTAGCTAAACATATGACTCGTTTAGTACATGGAGATGAAAAATTATCAGCTGCAGAAAGAATT
ACAAATATTCTTTTTTTTAAAAATATTAATGATATACAGATATCTGATTTAGAACAATTAAGAAAAGATGGTGTTCCTGT
AGTGGAAACTTATAAAATAAAAGATTTACAAGAAGCTTTGGTTTTGTCTTCTTTAGCACAGTCTCGCACTCAAGCTAAAA
ATATGATAATATCAAATTCAATATCTATAAACACTAAAAAAATAGTCAATAAAAATTATATTATTGATGATAATGATAAA
TTATTTAATCAATTTACCTTACTTTCTAGAGGAAAAAAAAATCACTGTCTTATTTATTGGAATAAATAG

Upstream 100 bases:

>100_bases
GTAAAAAAGAAATTAGAATAAAATACTGTAACATTGCATTTTTAAAATGCTTAGTAACAATTATTGTTGTATCTATATTT
AAAATAAGGTTTTAAATAAA

Downstream 100 bases:

>100_bases
AATTTTAATTGATTTTTGCTATTTTAATCAATTGAAAAACTTTCTCCACAACCACAGAATGTTTTTATTCTAGGATTGCA
GTATTTAAATACTTCATTAA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MDLINELHQRNLIFHITNENKLKELIKNKYISLYCGFDPTEDSLHVGHLLPLITLKRFQVLGHKPIVLIGGATSLIGDPS
FKTKERLLKSDSFVEKCTLKISQQISFFLDFNSNFNSAIILNNNLWFKKINILQFLRDVGKYFSVNALINREAVKKRIKR
SDQGISFTEFSYNLLQAYDFFILNQKNQVSLQIGGSDQWGNISSGMHLIHRISKKEAYGLTLPLLMQSNGIKFGKTESGT
VWLDPQKTTPYRFYQFWMNIEDSNVYRFLKLFTFIDIHEINEREKNSYKKNQIVSDKVLLAKHMTRLVHGDEKLSAAERI
TNILFFKNINDIQISDLEQLRKDGVPVVETYKIKDLQEALVLSSLAQSRTQAKNMIISNSISINTKKIVNKNYIIDDNDK
LFNQFTLLSRGKKNHCLIYWNK

Sequences:

>Translated_422_residues
MDLINELHQRNLIFHITNENKLKELIKNKYISLYCGFDPTEDSLHVGHLLPLITLKRFQVLGHKPIVLIGGATSLIGDPS
FKTKERLLKSDSFVEKCTLKISQQISFFLDFNSNFNSAIILNNNLWFKKINILQFLRDVGKYFSVNALINREAVKKRIKR
SDQGISFTEFSYNLLQAYDFFILNQKNQVSLQIGGSDQWGNISSGMHLIHRISKKEAYGLTLPLLMQSNGIKFGKTESGT
VWLDPQKTTPYRFYQFWMNIEDSNVYRFLKLFTFIDIHEINEREKNSYKKNQIVSDKVLLAKHMTRLVHGDEKLSAAERI
TNILFFKNINDIQISDLEQLRKDGVPVVETYKIKDLQEALVLSSLAQSRTQAKNMIISNSISINTKKIVNKNYIIDDNDK
LFNQFTLLSRGKKNHCLIYWNK
>Mature_422_residues
MDLINELHQRNLIFHITNENKLKELIKNKYISLYCGFDPTEDSLHVGHLLPLITLKRFQVLGHKPIVLIGGATSLIGDPS
FKTKERLLKSDSFVEKCTLKISQQISFFLDFNSNFNSAIILNNNLWFKKINILQFLRDVGKYFSVNALINREAVKKRIKR
SDQGISFTEFSYNLLQAYDFFILNQKNQVSLQIGGSDQWGNISSGMHLIHRISKKEAYGLTLPLLMQSNGIKFGKTESGT
VWLDPQKTTPYRFYQFWMNIEDSNVYRFLKLFTFIDIHEINEREKNSYKKNQIVSDKVLLAKHMTRLVHGDEKLSAAERI
TNILFFKNINDIQISDLEQLRKDGVPVVETYKIKDLQEALVLSSLAQSRTQAKNMIISNSISINTKKIVNKNYIIDDNDK
LFNQFTLLSRGKKNHCLIYWNK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=403, Percent_Identity=35.7320099255583, Blast_Score=242, Evalue=5e-64,
Organism=Escherichia coli, GI1787925, Length=419, Percent_Identity=55.1312649164678, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI25145802, Length=442, Percent_Identity=33.0316742081448, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI25145805, Length=340, Percent_Identity=31.4705882352941, Blast_Score=148, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6325160, Length=443, Percent_Identity=33.4085778781038, Blast_Score=235, Evalue=8e-63,
Organism=Drosophila melanogaster, GI20130383, Length=425, Percent_Identity=34.8235294117647, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_BUCAP (Q8KA14)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660471.1
- ProteinModelPortal:   Q8KA14
- SMR:   Q8KA14
- EnsemblBacteria:   EBBUCT00000000106
- GeneID:   1005930
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg113
- GeneTree:   EBGT00050000007861
- HOGENOM:   HBG288125
- OMA:   RQQDDSV
- ProtClustDB:   PRK05912
- BioCyc:   BAPH198804:BUSG113-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 48985; Mature: 48985

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 34-34 BINDING 172-172 BINDING 176-176 BINDING 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLINELHQRNLIFHITNENKLKELIKNKYISLYCGFDPTEDSLHVGHLLPLITLKRFQV
CCHHHHHHHCCEEEEECCCHHHHHHHHCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
LGHKPIVLIGGATSLIGDPSFKTKERLLKSDSFVEKCTLKISQQISFFLDFNSNFNSAII
CCCCCEEEECCCHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEEEEECCCCCEEEE
LNNNLWFKKINILQFLRDVGKYFSVNALINREAVKKRIKRSDQGISFTEFSYNLLQAYDF
EECCEEEEHHHHHHHHHHHHHHEEHHHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHEE
FILNQKNQVSLQIGGSDQWGNISSGMHLIHRISKKEAYGLTLPLLMQSNGIKFGKTESGT
EEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCCCCE
VWLDPQKTTPYRFYQFWMNIEDSNVYRFLKLFTFIDIHEINEREKNSYKKNQIVSDKVLL
EEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AKHMTRLVHGDEKLSAAERITNILFFKNINDIQISDLEQLRKDGVPVVETYKIKDLQEAL
HHHHHHHHCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHH
VLSSLAQSRTQAKNMIISNSISINTKKIVNKNYIIDDNDKLFNQFTLLSRGKKNHCLIYW
HHHHHHHHHHHHHHEEEECCCCCCHHHCCCCCCEECCCHHHHHHHHHHHCCCCCCEEEEE
NK
CC
>Mature Secondary Structure
MDLINELHQRNLIFHITNENKLKELIKNKYISLYCGFDPTEDSLHVGHLLPLITLKRFQV
CCHHHHHHHCCEEEEECCCHHHHHHHHCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
LGHKPIVLIGGATSLIGDPSFKTKERLLKSDSFVEKCTLKISQQISFFLDFNSNFNSAII
CCCCCEEEECCCHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEEEEECCCCCEEEE
LNNNLWFKKINILQFLRDVGKYFSVNALINREAVKKRIKRSDQGISFTEFSYNLLQAYDF
EECCEEEEHHHHHHHHHHHHHHEEHHHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHEE
FILNQKNQVSLQIGGSDQWGNISSGMHLIHRISKKEAYGLTLPLLMQSNGIKFGKTESGT
EEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECCCCCCE
VWLDPQKTTPYRFYQFWMNIEDSNVYRFLKLFTFIDIHEINEREKNSYKKNQIVSDKVLL
EEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AKHMTRLVHGDEKLSAAERITNILFFKNINDIQISDLEQLRKDGVPVVETYKIKDLQEAL
HHHHHHHHCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHH
VLSSLAQSRTQAKNMIISNSISINTKKIVNKNYIIDDNDKLFNQFTLLSRGKKNHCLIYW
HHHHHHHHHHHHHHEEEECCCCCCHHHCCCCCCEECCCHHHHHHHHHHHCCCCCCEEEEE
NK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438