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The map label for this gene is priA

Identifier: 21672403

GI number: 21672403

Start: 124043

End: 126205

Strand: Direct

Name: priA

Synonym: BUsg112

Alternate gene names: 21672403

Gene position: 124043-126205 (Clockwise)

Preceding gene: 21672402

Following gene: 21672404

Centisome position: 19.34

GC content: 18.26

Gene sequence:

>2163_bases
GTGATTATTGTAAAAGTTGTTTTACCTTTACCTATTAGAAAGTATTTTAAATATTTTATGCCTGATTCTATGTGTCCTAT
TATTGGTGGGCGAATCGTTGTGCCGTTTCGTTCTAAAGATATAGTAGGAATTGTAATTTCATTTTGTAATAAAAAAAATA
TAAGTAATTTAAATTTAGCATTTGTTAAATCATGTATTGATACTGAGTCTATTTATAGTGATGTTGTATTTAGTATTTTA
ATATGGTTAAGTAGATATTACTATTTTCCTATAGGAAGCATATTTTTTTCAATATTGCCAAAATATTTAAAAAAAATCTG
TTTAATAGATAATAAAAATTATAAATTTGCTATTTTAAGAAAAACAAAATATAAAGATTTTAAAACATTTAATCTTTTAT
TTTTTTGCAAAAAAAAAAGTTTTATTGACAAAGATTTAGAAAAATATACTTTTTTTGATTTTTTTTTAAAAAAAAATTTT
TTGCAAAAATCATGTAAAAATTATTTTTATCATGAAAATATTCCTCATATATATCAAAATTATTTAATTAAAAAAAAATT
TTTTTTAAATAAAAAAATAATTTTTATAATTAATAAAATTTTAATGAAAAATTGTTTTACATCTTGGCTAATAACTAAAA
ATAATTTTTATTTAAAAGTTAAATTTTATTTAGGTTTAATTAAAGAGTGTTTAAGTAAAAATTTACAAATTTTAATTTTA
GTCCCCTTTGTTAAGGATATATATCAAATATTATTTTTTTTAAAAAAATACTTTAATGTATATATTGATATTATTCATTC
TCAATTAAATAATGAAGATTATTTAAAAAAATGGATTAGAACAAAAAGTGGTAAAAATTCTATTATTATAGGCACAAAAA
ATAGTGTTTTTTTCCCTTTTTTAAAACTAGGTTTAATTATTGTTAATCAAGAACATCATTTGAACTATAGAAATTTAGAT
CAATGTAGATATAATGTTAGAGATATAGCAATATTAAGAGCCTATAAACAAAATATACCAATTATTTTAGATTCTGATAC
TCCTTCTTTAAGAACATTATATAATATTTTACATAAAAAATGTTTTTATATTAAATTTATAAAAAATAAAAAAACATTTT
TTTTAAAAAATAATGTTATTGATTTAAGAAAAGAGAGAATAAAAATAGGTTTATCTTCGACTCTAATAAATGAAATATTT
AATAATATTCAAAAAAATTATCCAGTATTATTAGTTTTGAATAAATTTAGTTTTGTTTTTTTTGGTTTAATATGCAGAAG
ATGCGGCAAGATAGAAAAATGTCATATTTGCAATGATTATTTTGAAACGAAAAAATACGATAATTTTTTATTTTGTCGAA
ATTGTTTAATCAAAATAAAAAAACCATTGTTTTGCTATAATTGTAAAAATTTTTCTTTAATCGTATTTGATTTTGGCATA
AAAAAAATAAAAAATAGTTTCAAGAAGATTTTTCCTAACATAAATTTATTTTTTTTATTAAGCTTAAAAAAAAACAAAAC
AAAAAAATTAAAAATACAATTTTTTAAATTTCCTATTTCTAATGCTTGTATTATTATCACTACTGAAAAAATTTCTCAAC
ATTACTATTTTCCTTATGTTAGATTTATTGCTTTAACAAACGTTGATCATTATTTTTTTTCTTTTCATTTTTGTTCTATA
GAACACTTTTTACAATTTTACTTTAATTTAATTAATTTAACGGGAGAAAATAAAAAATTATTAAAAATATTTATACAGAC
GTCTTATCCTAATAATAAATTTTTATTAAATCTGTGTAGTAGTGATTATTTTTTATTTTGTCGTAAGATATTATCTTTAA
GAAAAAAATATTTTTTACCGCCTTGGAATTTTCAAGTAATTTTTTACTCTTCAAGTAAATTTTTCGAAAAAAGTTTTATT
TTTTTAGAATGTATACAAATAATTTTAAAGAAACAATCTAAAAGAGATAACGTTTCTTTATGGTTTGTAGGTCCTCATCC
TGTATTTTCATTGAAAGATAGAAAAAAATGTTTTTATCAATTATTAATACACTCTCCTTCACGTACTTATTTAAAAAAAA
TATTAAAAGAATCAATTAATATAGTTCAATGTTTTTCTATATCGCAAAATGTTCAATGGTTTTTAGATATTGACATTTAT
TAA

Upstream 100 bases:

>100_bases
TTTGTACTGCTCGTCAATTGAAATGTAAAACATGTTTCATAGAAAAATTATGCGAATTTGACAAAAAAAAATAAGCACTC
TATTTATTTTGGGTACATAA

Downstream 100 bases:

>100_bases
ATTGAAAATTACTAAAAAATATTCAATTTTATTGAAATATCTTCTTTATTGAAGTAATTGAAGTAAAAAAGAAATTAGAA
TAAAATACTGTAACATTGCA

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MIIVKVVLPLPIRKYFKYFMPDSMCPIIGGRIVVPFRSKDIVGIVISFCNKKNISNLNLAFVKSCIDTESIYSDVVFSIL
IWLSRYYYFPIGSIFFSILPKYLKKICLIDNKNYKFAILRKTKYKDFKTFNLLFFCKKKSFIDKDLEKYTFFDFFLKKNF
LQKSCKNYFYHENIPHIYQNYLIKKKFFLNKKIIFIINKILMKNCFTSWLITKNNFYLKVKFYLGLIKECLSKNLQILIL
VPFVKDIYQILFFLKKYFNVYIDIIHSQLNNEDYLKKWIRTKSGKNSIIIGTKNSVFFPFLKLGLIIVNQEHHLNYRNLD
QCRYNVRDIAILRAYKQNIPIILDSDTPSLRTLYNILHKKCFYIKFIKNKKTFFLKNNVIDLRKERIKIGLSSTLINEIF
NNIQKNYPVLLVLNKFSFVFFGLICRRCGKIEKCHICNDYFETKKYDNFLFCRNCLIKIKKPLFCYNCKNFSLIVFDFGI
KKIKNSFKKIFPNINLFFLLSLKKNKTKKLKIQFFKFPISNACIIITTEKISQHYYFPYVRFIALTNVDHYFFSFHFCSI
EHFLQFYFNLINLTGENKKLLKIFIQTSYPNNKFLLNLCSSDYFLFCRKILSLRKKYFLPPWNFQVIFYSSSKFFEKSFI
FLECIQIILKKQSKRDNVSLWFVGPHPVFSLKDRKKCFYQLLIHSPSRTYLKKILKESINIVQCFSISQNVQWFLDIDIY

Sequences:

>Translated_720_residues
MIIVKVVLPLPIRKYFKYFMPDSMCPIIGGRIVVPFRSKDIVGIVISFCNKKNISNLNLAFVKSCIDTESIYSDVVFSIL
IWLSRYYYFPIGSIFFSILPKYLKKICLIDNKNYKFAILRKTKYKDFKTFNLLFFCKKKSFIDKDLEKYTFFDFFLKKNF
LQKSCKNYFYHENIPHIYQNYLIKKKFFLNKKIIFIINKILMKNCFTSWLITKNNFYLKVKFYLGLIKECLSKNLQILIL
VPFVKDIYQILFFLKKYFNVYIDIIHSQLNNEDYLKKWIRTKSGKNSIIIGTKNSVFFPFLKLGLIIVNQEHHLNYRNLD
QCRYNVRDIAILRAYKQNIPIILDSDTPSLRTLYNILHKKCFYIKFIKNKKTFFLKNNVIDLRKERIKIGLSSTLINEIF
NNIQKNYPVLLVLNKFSFVFFGLICRRCGKIEKCHICNDYFETKKYDNFLFCRNCLIKIKKPLFCYNCKNFSLIVFDFGI
KKIKNSFKKIFPNINLFFLLSLKKNKTKKLKIQFFKFPISNACIIITTEKISQHYYFPYVRFIALTNVDHYFFSFHFCSI
EHFLQFYFNLINLTGENKKLLKIFIQTSYPNNKFLLNLCSSDYFLFCRKILSLRKKYFLPPWNFQVIFYSSSKFFEKSFI
FLECIQIILKKQSKRDNVSLWFVGPHPVFSLKDRKKCFYQLLIHSPSRTYLKKILKESINIVQCFSISQNVQWFLDIDIY
>Mature_720_residues
MIIVKVVLPLPIRKYFKYFMPDSMCPIIGGRIVVPFRSKDIVGIVISFCNKKNISNLNLAFVKSCIDTESIYSDVVFSIL
IWLSRYYYFPIGSIFFSILPKYLKKICLIDNKNYKFAILRKTKYKDFKTFNLLFFCKKKSFIDKDLEKYTFFDFFLKKNF
LQKSCKNYFYHENIPHIYQNYLIKKKFFLNKKIIFIINKILMKNCFTSWLITKNNFYLKVKFYLGLIKECLSKNLQILIL
VPFVKDIYQILFFLKKYFNVYIDIIHSQLNNEDYLKKWIRTKSGKNSIIIGTKNSVFFPFLKLGLIIVNQEHHLNYRNLD
QCRYNVRDIAILRAYKQNIPIILDSDTPSLRTLYNILHKKCFYIKFIKNKKTFFLKNNVIDLRKERIKIGLSSTLINEIF
NNIQKNYPVLLVLNKFSFVFFGLICRRCGKIEKCHICNDYFETKKYDNFLFCRNCLIKIKKPLFCYNCKNFSLIVFDFGI
KKIKNSFKKIFPNINLFFLLSLKKNKTKKLKIQFFKFPISNACIIITTEKISQHYYFPYVRFIALTNVDHYFFSFHFCSI
EHFLQFYFNLINLTGENKKLLKIFIQTSYPNNKFLLNLCSSDYFLFCRKILSLRKKYFLPPWNFQVIFYSSSKFFEKSFI
FLECIQIILKKQSKRDNVSLWFVGPHPVFSLKDRKKCFYQLLIHSPSRTYLKKILKESINIVQCFSISQNVQWFLDIDIY

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase ATP-binding domain

Homologues:

Organism=Escherichia coli, GI1790370, Length=730, Percent_Identity=26.8493150684932, Blast_Score=380, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIA_BUCAP (Q8KA15)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660470.1
- ProteinModelPortal:   Q8KA15
- SMR:   Q8KA15
- EnsemblBacteria:   EBBUCT00000000034
- GeneID:   1005929
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg112
- GeneTree:   EBGT00050000007720
- HOGENOM:   HBG366203
- OMA:   RTIRIDS
- ProtClustDB:   CLSK315414
- BioCyc:   BAPH198804:BUSG112-MONOMER
- InterPro:   IPR014021
- InterPro:   IPR005259
- TIGRFAMs:   TIGR00595

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 86673; Mature: 86673

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS51192 HELICASE_ATP_BIND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVKVVLPLPIRKYFKYFMPDSMCPIIGGRIVVPFRSKDIVGIVISFCNKKNISNLNLA
CEEEEEECCCCHHHHHHHHCCCCCCHHHCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHH
FVKSCIDTESIYSDVVFSILIWLSRYYYFPIGSIFFSILPKYLKKICLIDNKNYKFAILR
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCEEEEEE
KTKYKDFKTFNLLFFCKKKSFIDKDLEKYTFFDFFLKKNFLQKSCKNYFYHENIPHIYQN
ECCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
YLIKKKFFLNKKIIFIINKILMKNCFTSWLITKNNFYLKVKFYLGLIKECLSKNLQILIL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCEEEEEHHHHHHHHHHHCCCCEEEEE
VPFVKDIYQILFFLKKYFNVYIDIIHSQLNNEDYLKKWIRTKSGKNSIIIGTKNSVFFPF
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCHHHH
LKLGLIIVNQEHHLNYRNLDQCRYNVRDIAILRAYKQNIPIILDSDTPSLRTLYNILHKK
HHCCEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHH
CFYIKFIKNKKTFFLKNNVIDLRKERIKIGLSSTLINEIFNNIQKNYPVLLVLNKFSFVF
EEEEEEECCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHH
FGLICRRCGKIEKCHICNDYFETKKYDNFLFCRNCLIKIKKPLFCYNCKNFSLIVFDFGI
HHHHHHHCCCCCEEEECHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEEECCH
KKIKNSFKKIFPNINLFFLLSLKKNKTKKLKIQFFKFPISNACIIITTEKISQHYYFPYV
HHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEEEECCCCEEEEEEEHHHCCCCCCHHE
RFIALTNVDHYFFSFHFCSIEHFLQFYFNLINLTGENKKLLKIFIQTSYPNNKFLLNLCS
EEHHHCCCCCEEHEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEEECC
SDYFLFCRKILSLRKKYFLPPWNFQVIFYSSSKFFEKSFIFLECIQIILKKQSKRDNVSL
CCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEE
WFVGPHPVFSLKDRKKCFYQLLIHSPSRTYLKKILKESINIVQCFSISQNVQWFLDIDIY
EEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEEC
>Mature Secondary Structure
MIIVKVVLPLPIRKYFKYFMPDSMCPIIGGRIVVPFRSKDIVGIVISFCNKKNISNLNLA
CEEEEEECCCCHHHHHHHHCCCCCCHHHCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHH
FVKSCIDTESIYSDVVFSILIWLSRYYYFPIGSIFFSILPKYLKKICLIDNKNYKFAILR
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCEEEEEE
KTKYKDFKTFNLLFFCKKKSFIDKDLEKYTFFDFFLKKNFLQKSCKNYFYHENIPHIYQN
ECCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
YLIKKKFFLNKKIIFIINKILMKNCFTSWLITKNNFYLKVKFYLGLIKECLSKNLQILIL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCEEEEEHHHHHHHHHHHCCCCEEEEE
VPFVKDIYQILFFLKKYFNVYIDIIHSQLNNEDYLKKWIRTKSGKNSIIIGTKNSVFFPF
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCHHHH
LKLGLIIVNQEHHLNYRNLDQCRYNVRDIAILRAYKQNIPIILDSDTPSLRTLYNILHKK
HHCCEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHH
CFYIKFIKNKKTFFLKNNVIDLRKERIKIGLSSTLINEIFNNIQKNYPVLLVLNKFSFVF
EEEEEEECCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHH
FGLICRRCGKIEKCHICNDYFETKKYDNFLFCRNCLIKIKKPLFCYNCKNFSLIVFDFGI
HHHHHHHCCCCCEEEECHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEEECCH
KKIKNSFKKIFPNINLFFLLSLKKNKTKKLKIQFFKFPISNACIIITTEKISQHYYFPYV
HHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEEEECCCCEEEEEEEHHHCCCCCCHHE
RFIALTNVDHYFFSFHFCSIEHFLQFYFNLINLTGENKKLLKIFIQTSYPNNKFLLNLCS
EEHHHCCCCCEEHEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEEECC
SDYFLFCRKILSLRKKYFLPPWNFQVIFYSSSKFFEKSFIFLECIQIILKKQSKRDNVSL
CCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEE
WFVGPHPVFSLKDRKKCFYQLLIHSPSRTYLKKILKESINIVQCFSISQNVQWFLDIDIY
EEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438