Click here to switch to the map view.

The map label for this gene is lipB

Identifier: 21241438

GI number: 21241438

Start: 791009

End: 791707

Strand: Direct

Name: lipB

Synonym: XAC0667

Alternate gene names: 21241438

Gene position: 791009-791707 (Clockwise)

Preceding gene: 21241437

Following gene: 21241439

Centisome position: 15.28

GC content: 65.52

Gene sequence:

>699_bases
GTGGACGCTGTAGCGGCCGAGCCTGTGAGCAGCTCCATGGCTGGCCTGCCTGCGCAATTGCGCGACCTCGGCCAGCAAGA
CTACGCGCCTGTGTGGCGTGCAATGCAGCGTTTCACCGATGCGCGCGAAGAGTACACCGCCGATGAGATCTGGGTGGTCG
AGCATGCGCCGGTGTTTACGTTGGGTCAGGCGGGCAAACCCGAACATGTCCTGGCACCGGGCGAGATTCCGGTGCTACAG
GTCGATCGCGGCGGGCAGGTCACGTATCACGGCCCTGGCCAATTGGTGGTGTACCCGCTGCTGGACCTGCGCCGGCTGAA
GATCGGCGTGCGCGATTACGTGTGCAAGATCGAACAGGCGCTGATCGATACGCTGGACGAGTGGAACATCGTGGCCGAGC
GCCGCGACGGTGCTCCTGGCGTGTATGTGGGCGGGGCCAAGATCGCCGCGCTTGGGATCCGGGTGCGGCGTGGCTGCACC
TTCCATGGGTTGTCGTTCAACGTTGCGATGGACCTGCAACCGTTCCACCGCATCAACCCGTGCGGCTACCAGGGTCTGCA
GGTGACCTCGGTGCTAGACTTGGGCGGCCCCTCCGGGATGGACGCCGTCAAGGCGGTGCTGCTCGACCAGCTGGCGCGCC
AGTTCGGCCTCGTGCTGCAGCCCACTTCCGCATTGCCCGACCTTTCGCTTCCGGCCTGA

Upstream 100 bases:

>100_bases
CCAGCGGAAAGTATGTCTCGGTCAAGATTGGCTTCCGTGCCCAAAGCCGCGAGCAGTTCGAGGCCGCGCACCAGGCGCTG
CGCGATCATCCGGAAGTGAA

Downstream 100 bases:

>100_bases
GCGCCCCGTATCGTCATGACCCAGCCTATCGCCCGTTCCATTCCCTTGCAGGTCGTCTCTGGCGACACCGCCGCGCCTGC
GCCGCTGCAGACTGGCGTCA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MDAVAAEPVSSSMAGLPAQLRDLGQQDYAPVWRAMQRFTDAREEYTADEIWVVEHAPVFTLGQAGKPEHVLAPGEIPVLQ
VDRGGQVTYHGPGQLVVYPLLDLRRLKIGVRDYVCKIEQALIDTLDEWNIVAERRDGAPGVYVGGAKIAALGIRVRRGCT
FHGLSFNVAMDLQPFHRINPCGYQGLQVTSVLDLGGPSGMDAVKAVLLDQLARQFGLVLQPTSALPDLSLPA

Sequences:

>Translated_232_residues
MDAVAAEPVSSSMAGLPAQLRDLGQQDYAPVWRAMQRFTDAREEYTADEIWVVEHAPVFTLGQAGKPEHVLAPGEIPVLQ
VDRGGQVTYHGPGQLVVYPLLDLRRLKIGVRDYVCKIEQALIDTLDEWNIVAERRDGAPGVYVGGAKIAALGIRVRRGCT
FHGLSFNVAMDLQPFHRINPCGYQGLQVTSVLDLGGPSGMDAVKAVLLDQLARQFGLVLQPTSALPDLSLPA
>Mature_232_residues
MDAVAAEPVSSSMAGLPAQLRDLGQQDYAPVWRAMQRFTDAREEYTADEIWVVEHAPVFTLGQAGKPEHVLAPGEIPVLQ
VDRGGQVTYHGPGQLVVYPLLDLRRLKIGVRDYVCKIEQALIDTLDEWNIVAERRDGAPGVYVGGAKIAALGIRVRRGCT
FHGLSFNVAMDLQPFHRINPCGYQGLQVTSVLDLGGPSGMDAVKAVLLDQLARQFGLVLQPTSALPDLSLPA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=145, Percent_Identity=37.9310344827586, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI87081767, Length=192, Percent_Identity=55.7291666666667, Blast_Score=229, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6323268, Length=179, Percent_Identity=31.2849162011173, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21355483, Length=184, Percent_Identity=35.3260869565217, Blast_Score=103, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_XANAC (Q8PPL9)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641020.1
- ProteinModelPortal:   Q8PPL9
- SMR:   Q8PPL9
- GeneID:   1154738
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0667
- NMPDR:   fig|190486.1.peg.664
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- ProtClustDB:   PRK14342
- BioCyc:   XAXO190486:XAC0667-MONOMER
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 25164; Mature: 25164

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVAAEPVSSSMAGLPAQLRDLGQQDYAPVWRAMQRFTDAREEYTADEIWVVEHAPVFT
CCCCCCCCCHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEE
LGQAGKPEHVLAPGEIPVLQVDRGGQVTYHGPGQLVVYPLLDLRRLKIGVRDYVCKIEQA
ECCCCCCCEEECCCCCCEEEECCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LIDTLDEWNIVAERRDGAPGVYVGGAKIAALGIRVRRGCTFHGLSFNVAMDLQPFHRINP
HHHHHHHCCEEEECCCCCCEEEECCCEEEEEHEEHHCCCEEECCEEEEEECCCCCCCCCC
CGYQGLQVTSVLDLGGPSGMDAVKAVLLDQLARQFGLVLQPTSALPDLSLPA
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MDAVAAEPVSSSMAGLPAQLRDLGQQDYAPVWRAMQRFTDAREEYTADEIWVVEHAPVFT
CCCCCCCCCHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEE
LGQAGKPEHVLAPGEIPVLQVDRGGQVTYHGPGQLVVYPLLDLRRLKIGVRDYVCKIEQA
ECCCCCCCEEECCCCCCEEEECCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LIDTLDEWNIVAERRDGAPGVYVGGAKIAALGIRVRRGCTFHGLSFNVAMDLQPFHRINP
HHHHHHHCCEEEECCCCCCEEEECCCEEEEEHEEHHCCCEEECCEEEEEECCCCCCCCCC
CGYQGLQVTSVLDLGGPSGMDAVKAVLLDQLARQFGLVLQPTSALPDLSLPA
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217