Click here to switch to the map view.

The map label for this gene is lipA

Identifier: 21241439

GI number: 21241439

Start: 791723

End: 792736

Strand: Direct

Name: lipA

Synonym: XAC0668

Alternate gene names: 21241439

Gene position: 791723-792736 (Clockwise)

Preceding gene: 21241438

Following gene: 21241440

Centisome position: 15.3

GC content: 65.58

Gene sequence:

>1014_bases
ATGACCCAGCCTATCGCCCGTTCCATTCCCTTGCAGGTCGTCTCTGGCGACACCGCCGCGCCTGCGCCGCTGCAGACTGG
CGTCAAGCAGATCGGTGGCGACAAGATCAACCGCTCGCCGGTGCAGTTCGTGGATGCGCCGGTGCTGCGGAAACCGTCGT
GGATCCGGGTGCGGATTCCGTCTGGCAATGCGGTACAGAACCTCAAGGCCAAGCTGCGCGAAAACCGCCTGGTCACGGTG
TGCGAAGAAGCCAGCTGCCCGAACATCCACGAGTGCTTCAGCCACGGCACCGCGACCTTCATGATTTTGGGCGAGGTGTG
CACGCGGCGCTGCTCGTTCTGCGACGTGGCGCATGGCCGGCCAAAGCCGCCCGACGCGAGCGAACCTGCCAGCCTGGCCG
CCACTGTTGCCGACATGGGCCTGAAGTACGTGGTGGTGACCAGCGTGGATCGTGACGACCTGCGCGACGGTGGCGCGCAG
CATTTCGTTGACTGCATCTCGGCGATCCGGACCAGCTCGCCCAACACGCGTATCGAAATCCTGACCCCGGACTTCCGCGG
CAAGGGCCGCATGGACCGCGCGCTGGAGATCCTGGCGCTGAGCCCGCCGGACGTGTTCAACCACAACATCGAAACCGTGC
CGGACCTCTACCCGAATGTGCGCCCCGGCGCGGACTACCAGTGGTCGCTGACGCTGTTGCAGCGCTTCAAGGCGCAGCAT
CCGTCCATCGCCACCAAGTCCGGCATCATGCTGGGCCTGGGCGAGACGATGGAGCAGGTGCAGGCCACGCTGCGCGACCT
GCGCGCGCACGATGTGGACATGATCACCATCGGCCAGTATCTGCAGCCGACGCCGCACCATCATCCGGTGATGCGTTACT
GGACGCCTGAGGAATACAAGGCGCTGGAAGACTACGGCAACGCGCTGGGCTTCAGTCATGTTGCCTCCGGCCCGATGGTG
CGTTCCTCGTACCACGCCGATCGTCAGGCCGCAGGCGCCGGCGTCGCCGCCTGA

Upstream 100 bases:

>100_bases
GGTGCTGCTCGACCAGCTGGCGCGCCAGTTCGGCCTCGTGCTGCAGCCCACTTCCGCATTGCCCGACCTTTCGCTTCCGG
CCTGAGCGCCCCGTATCGTC

Downstream 100 bases:

>100_bases
GTCGGCAACAGGCGATCGGACGAGTGCCGCGGCGGGTGGGCACCGGTGCCCGCAGCAGCACCGCGATGCTGGTGCGCTCA
ATGCCGGCCGGTCTCGCGTG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTQPIARSIPLQVVSGDTAAPAPLQTGVKQIGGDKINRSPVQFVDAPVLRKPSWIRVRIPSGNAVQNLKAKLRENRLVTV
CEEASCPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGRPKPPDASEPASLAATVADMGLKYVVVTSVDRDDLRDGGAQ
HFVDCISAIRTSSPNTRIEILTPDFRGKGRMDRALEILALSPPDVFNHNIETVPDLYPNVRPGADYQWSLTLLQRFKAQH
PSIATKSGIMLGLGETMEQVQATLRDLRAHDVDMITIGQYLQPTPHHHPVMRYWTPEEYKALEDYGNALGFSHVASGPMV
RSSYHADRQAAGAGVAA

Sequences:

>Translated_337_residues
MTQPIARSIPLQVVSGDTAAPAPLQTGVKQIGGDKINRSPVQFVDAPVLRKPSWIRVRIPSGNAVQNLKAKLRENRLVTV
CEEASCPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGRPKPPDASEPASLAATVADMGLKYVVVTSVDRDDLRDGGAQ
HFVDCISAIRTSSPNTRIEILTPDFRGKGRMDRALEILALSPPDVFNHNIETVPDLYPNVRPGADYQWSLTLLQRFKAQH
PSIATKSGIMLGLGETMEQVQATLRDLRAHDVDMITIGQYLQPTPHHHPVMRYWTPEEYKALEDYGNALGFSHVASGPMV
RSSYHADRQAAGAGVAA
>Mature_336_residues
TQPIARSIPLQVVSGDTAAPAPLQTGVKQIGGDKINRSPVQFVDAPVLRKPSWIRVRIPSGNAVQNLKAKLRENRLVTVC
EEASCPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGRPKPPDASEPASLAATVADMGLKYVVVTSVDRDDLRDGGAQH
FVDCISAIRTSSPNTRIEILTPDFRGKGRMDRALEILALSPPDVFNHNIETVPDLYPNVRPGADYQWSLTLLQRFKAQHP
SIATKSGIMLGLGETMEQVQATLRDLRAHDVDMITIGQYLQPTPHHHPVMRYWTPEEYKALEDYGNALGFSHVASGPMVR
SSYHADRQAAGAGVAA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=50.8771929824561, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI37577164, Length=249, Percent_Identity=49.7991967871486, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=60.4361370716511, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32564533, Length=289, Percent_Identity=46.3667820069204, Blast_Score=235, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=282, Percent_Identity=47.8723404255319, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI221513272, Length=285, Percent_Identity=48.7719298245614, Blast_Score=272, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_XANAC (Q8PPL8)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641021.1
- ProteinModelPortal:   Q8PPL8
- SMR:   Q8PPL8
- GeneID:   1154739
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0668
- NMPDR:   fig|190486.1.peg.665
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   XAXO190486:XAC0668-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36864; Mature: 36733

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPIARSIPLQVVSGDTAAPAPLQTGVKQIGGDKINRSPVQFVDAPVLRKPSWIRVRIP
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCEEEEECC
SGNAVQNLKAKLRENRLVTVCEEASCPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGR
CCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCCCEEHHHHHHHHHCCHHHCCCCC
PKPPDASEPASLAATVADMGLKYVVVTSVDRDDLRDGGAQHFVDCISAIRTSSPNTRIEI
CCCCCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEE
LTPDFRGKGRMDRALEILALSPPDVFNHNIETVPDLYPNVRPGADYQWSLTLLQRFKAQH
ECCCCCCCCCCCHHEEEEECCCCHHHCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCC
PSIATKSGIMLGLGETMEQVQATLRDLRAHDVDMITIGQYLQPTPHHHPVMRYWTPEEYK
CCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEECHHCCCCCCCCCEEECCCCHHHH
ALEDYGNALGFSHVASGPMVRSSYHADRQAAGAGVAA
HHHHHHHHCCHHHHCCCCCCHHHHCCHHHHCCCCCCC
>Mature Secondary Structure 
TQPIARSIPLQVVSGDTAAPAPLQTGVKQIGGDKINRSPVQFVDAPVLRKPSWIRVRIP
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCEEEEECC
SGNAVQNLKAKLRENRLVTVCEEASCPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGR
CCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCCCEEHHHHHHHHHCCHHHCCCCC
PKPPDASEPASLAATVADMGLKYVVVTSVDRDDLRDGGAQHFVDCISAIRTSSPNTRIEI
CCCCCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEE
LTPDFRGKGRMDRALEILALSPPDVFNHNIETVPDLYPNVRPGADYQWSLTLLQRFKAQH
ECCCCCCCCCCCHHEEEEECCCCHHHCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCC
PSIATKSGIMLGLGETMEQVQATLRDLRAHDVDMITIGQYLQPTPHHHPVMRYWTPEEYK
CCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEECHHCCCCCCCCCEEECCCCHHHH
ALEDYGNALGFSHVASGPMVRSSYHADRQAAGAGVAA
HHHHHHHHCCHHHHCCCCCCHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217