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The map label for this gene is hemB [H]

Identifier: 57237820

GI number: 57237820

Start: 1003657

End: 1004640

Strand: Reverse

Name: hemB [H]

Synonym: CJE1075

Alternate gene names: 57237820

Gene position: 1004640-1003657 (Counterclockwise)

Preceding gene: 57237823

Following gene: 57237819

Centisome position: 56.51

GC content: 33.64

Gene sequence:

>984_bases
ATGTTTAAAAGATTTAGAAGATTAAGGCTTAATGAAAACTTAAGATCTATGGTAAGGGAAAATTCTTTAAGTGTAAATGA
TTTGATTTATCCGCTTTTTGTGGTTAATGGAACGGGGATAAAAAAAGAAATTTCTTCTATGCCTGATGTGTTTCAAATGA
GTTTAGATGAAATTTTAAAAGAGTGTCAAAATGTAGTTAATTTAGGCATTAAAGCCATTATACTTTTTGGTGTTTTAGAA
AATGAGAAAAAAGACAGTTGCGGAAGCGATGCTTTAGATGATGAAGGGCTTGTTGCAAGAAGTATAAGAGAGATTAAGAA
AAATTTTCCAGATCTTTTTATAATCAGTGATCTTTGTTTTTGCGAATACACAGATCACGGGCATTGTGGGATTATCGATC
CAAAAACAAAAAGCGTAGATAATGATGCAACTTTAGAAATTTCAGCCAAGCAAGCCTTAGTTCATGCAAGAGCAGGGGTA
GATATGATAGCACCTAGTGGAATGATGGATGGTGTTATCACTACTTTGCGCAAAGCTTTAGATAAAGAAGGTTTTGAAAA
TTTGCCTATAATGGCGTATTCTACTAAATTTGCTTCAAGTTATTATGGGCCATTTCGAGATGTAGCAGAATCAGCTCCAA
GTTATGGAGATAGAAAAAGCTATCAAATGGATTTTGCTAATGGTAAAGAAGCTTTAGAAGAAAGCTTGGAAGATGAAGCA
CAAGGAGCTGATATTTTGATGGTAAAACCCGCTCTTGCTTATCTTGATGTGGTTAAAGAAATATCCCTTCATTCTAACTT
GCCTTTGTGTGTTTATAATGTAAGCGGAGAGTATGCTATGTTAAAGGCAGCTAAAAATGCAGGAGTGATTGATTATGAAA
AAGTTTTGTATGAAACTATGATAGCTTTTAAAAGAGCAGGGGCAAAGCTTATCATCACTTATCATGCCAAAGAATTAGCT
AAAATGTTAAAAGGAGAAAAATGA

Upstream 100 bases:

>100_bases
ATTTGCTAGGTAAATTCGCAATTTCTGAAATTTTTATTTCCATTTACTACTCTCTATAATCAAATTTATGTTAAGATTTT
AGCAAAAAAAGGACAAAAAA

Downstream 100 bases:

>100_bases
AACATTTTTTAACTTTAAGAGATTTTTCTAAGGAGGAAATTTTAAGCCTTGTAAATCATGCTAGCGAGCTTAAAAAAGAG
CCTAAAAAACTTTTACAAGA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MFKRFRRLRLNENLRSMVRENSLSVNDLIYPLFVVNGTGIKKEISSMPDVFQMSLDEILKECQNVVNLGIKAIILFGVLE
NEKKDSCGSDALDDEGLVARSIREIKKNFPDLFIISDLCFCEYTDHGHCGIIDPKTKSVDNDATLEISAKQALVHARAGV
DMIAPSGMMDGVITTLRKALDKEGFENLPIMAYSTKFASSYYGPFRDVAESAPSYGDRKSYQMDFANGKEALEESLEDEA
QGADILMVKPALAYLDVVKEISLHSNLPLCVYNVSGEYAMLKAAKNAGVIDYEKVLYETMIAFKRAGAKLIITYHAKELA
KMLKGEK

Sequences:

>Translated_327_residues
MFKRFRRLRLNENLRSMVRENSLSVNDLIYPLFVVNGTGIKKEISSMPDVFQMSLDEILKECQNVVNLGIKAIILFGVLE
NEKKDSCGSDALDDEGLVARSIREIKKNFPDLFIISDLCFCEYTDHGHCGIIDPKTKSVDNDATLEISAKQALVHARAGV
DMIAPSGMMDGVITTLRKALDKEGFENLPIMAYSTKFASSYYGPFRDVAESAPSYGDRKSYQMDFANGKEALEESLEDEA
QGADILMVKPALAYLDVVKEISLHSNLPLCVYNVSGEYAMLKAAKNAGVIDYEKVLYETMIAFKRAGAKLIITYHAKELA
KMLKGEK
>Mature_327_residues
MFKRFRRLRLNENLRSMVRENSLSVNDLIYPLFVVNGTGIKKEISSMPDVFQMSLDEILKECQNVVNLGIKAIILFGVLE
NEKKDSCGSDALDDEGLVARSIREIKKNFPDLFIISDLCFCEYTDHGHCGIIDPKTKSVDNDATLEISAKQALVHARAGV
DMIAPSGMMDGVITTLRKALDKEGFENLPIMAYSTKFASSYYGPFRDVAESAPSYGDRKSYQMDFANGKEALEESLEDEA
QGADILMVKPALAYLDVVKEISLHSNLPLCVYNVSGEYAMLKAAKNAGVIDYEKVLYETMIAFKRAGAKLIITYHAKELA
KMLKGEK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=308, Percent_Identity=39.9350649350649, Blast_Score=247, Evalue=9e-66,
Organism=Escherichia coli, GI87081728, Length=321, Percent_Identity=51.0903426791277, Blast_Score=311, Evalue=5e-86,
Organism=Saccharomyces cerevisiae, GI6321398, Length=317, Percent_Identity=40.0630914826498, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI21358291, Length=300, Percent_Identity=37, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36414; Mature: 36414

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRFRRLRLNENLRSMVRENSLSVNDLIYPLFVVNGTGIKKEISSMPDVFQMSLDEILK
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCCCHHHHHHCCHHHHHHHHHHHHH
ECQNVVNLGIKAIILFGVLENEKKDSCGSDALDDEGLVARSIREIKKNFPDLFIISDLCF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHH
CEYTDHGHCGIIDPKTKSVDNDATLEISAKQALVHARAGVDMIAPSGMMDGVITTLRKAL
EECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
DKEGFENLPIMAYSTKFASSYYGPFRDVAESAPSYGDRKSYQMDFANGKEALEESLEDEA
HHCCCCCCCEEEEEHHHHHHHCCHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHH
QGADILMVKPALAYLDVVKEISLHSNLPLCVYNVSGEYAMLKAAKNAGVIDYEKVLYETM
CCCCEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHCCCCCCHHHHHHHHHH
IAFKRAGAKLIITYHAKELAKMLKGEK
HHHHHCCCEEEEEECHHHHHHHHCCCC
>Mature Secondary Structure
MFKRFRRLRLNENLRSMVRENSLSVNDLIYPLFVVNGTGIKKEISSMPDVFQMSLDEILK
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCCCHHHHHHCCHHHHHHHHHHHHH
ECQNVVNLGIKAIILFGVLENEKKDSCGSDALDDEGLVARSIREIKKNFPDLFIISDLCF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHH
CEYTDHGHCGIIDPKTKSVDNDATLEISAKQALVHARAGVDMIAPSGMMDGVITTLRKAL
EECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
DKEGFENLPIMAYSTKFASSYYGPFRDVAESAPSYGDRKSYQMDFANGKEALEESLEDEA
HHCCCCCCCEEEEEHHHHHHHCCHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHH
QGADILMVKPALAYLDVVKEISLHSNLPLCVYNVSGEYAMLKAAKNAGVIDYEKVLYETM
CCCCEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHCCCCCCHHHHHHHHHH
IAFKRAGAKLIITYHAKELAKMLKGEK
HHHHHCCCEEEEEECHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]