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The map label for this gene is nadE [H]

Identifier: 21226714

GI number: 21226714

Start: 742973

End: 744037

Strand: Reverse

Name: nadE [H]

Synonym: MM_0612

Alternate gene names: 21226714

Gene position: 744037-742973 (Counterclockwise)

Preceding gene: 21226715

Following gene: 21226713

Centisome position: 18.16

GC content: 44.51

Gene sequence:

>1065_bases
ATGCAGATTGTTGGAAGTAGCGTAGAGAAAACTACGGAGAGTCATGTAAGGGCGTTATCAAAGAAAGGTGCCGGAAAGCA
AATAGATTATAGTGATGTTCTGATTCTGAACGAACTGAACAGGGATATCGGTGAGCTTGCCAAAAATATAAGGGGTTTTA
TAAGAGACCAGGTCACCGGCTTTAAGAAAAAAGGAGTGGTTCTGGGGGTATCGGGAGGTATAGATTCAGCTGTAGCCCTG
ACGCTATGTGTTCAGGAGTTCGGCAAAGAAAATGTATATGGTCTCCTCCTCCCTGAAAAAGAATCGGCTCCTTCAAGCAA
GACCCTGGGAGCAGAAATCTGTGAAAGCCTGGGTGTCCAGTATGAGGAGGTCCCTATTTCCCCAATCATTGAGGCTCTGG
GAATTTATGACAAAAAAGAACAGATAATTAAAAAAACCTGTCCTGAATACGACCCGAAAATACACAAAACATCTCTTGTG
CTGCCTGATTTCCTTAACCAGGGGCTGTTGAATGTCCCGTACATCAGGCTGATAAAGGACGGGGAAACTATCTCCAGATA
CAGGTTAAAAGCAAATGACTACCTGGAGTTAATTGGCCTTCAGAATGTCAAGCAGAGGTCAAGAATGATAGTCCAGTACA
TGTATGCCGAAAAGCTAAATTATGTTGTTTGCGGCACAACCAATAAAACCGAACTTGTCCTGGGCCAGTTTGTAAAATAC
GGGGACGGCGGTGTGGACCTCGAACCTCTGGCAGACTGCTATAAGACCCAGATCTATGCTCTGGCAAAATATCTGGAGGT
AAATGAGGAAATTATAAAGCGCCCTCCAAGTGCTGATACATGGAGTCATTACACAAGCGACGAGGAGTTCTACTGGCGTA
TGCCAATACATATCATGGACCAGCTCCTCTATGCCCAGGAACATAAACTGCCTTTAGAGGTTGCTGAAAGGAACACTGGC
CTTCCCGGAGATATCATAGAAAAAGCCTGGAGGCATATTGACAGAATAAGAGATACTACAGAATATGTGCGTTCGGCTCC
TCCTGTTTATTATCTGGATAGGTAA

Upstream 100 bases:

>100_bases
GATTTGAAATTATTATACAGAATTATAACCAGTTTACATAACAAATTTCTGAATTCATAACCAGTTTACATAACAAATAT
TCTGTTAGGGGAGTAGTTTT

Downstream 100 bases:

>100_bases
GCCGCAAAAACAGGTTCTACATCAAAATTTCATATGGATCTGATCCACAATTCAGATGGATAAGCATGAACACAACAGTC
TCAATTGTACGCTGTTCCGA

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MQIVGSSVEKTTESHVRALSKKGAGKQIDYSDVLILNELNRDIGELAKNIRGFIRDQVTGFKKKGVVLGVSGGIDSAVAL
TLCVQEFGKENVYGLLLPEKESAPSSKTLGAEICESLGVQYEEVPISPIIEALGIYDKKEQIIKKTCPEYDPKIHKTSLV
LPDFLNQGLLNVPYIRLIKDGETISRYRLKANDYLELIGLQNVKQRSRMIVQYMYAEKLNYVVCGTTNKTELVLGQFVKY
GDGGVDLEPLADCYKTQIYALAKYLEVNEEIIKRPPSADTWSHYTSDEEFYWRMPIHIMDQLLYAQEHKLPLEVAERNTG
LPGDIIEKAWRHIDRIRDTTEYVRSAPPVYYLDR

Sequences:

>Translated_354_residues
MQIVGSSVEKTTESHVRALSKKGAGKQIDYSDVLILNELNRDIGELAKNIRGFIRDQVTGFKKKGVVLGVSGGIDSAVAL
TLCVQEFGKENVYGLLLPEKESAPSSKTLGAEICESLGVQYEEVPISPIIEALGIYDKKEQIIKKTCPEYDPKIHKTSLV
LPDFLNQGLLNVPYIRLIKDGETISRYRLKANDYLELIGLQNVKQRSRMIVQYMYAEKLNYVVCGTTNKTELVLGQFVKY
GDGGVDLEPLADCYKTQIYALAKYLEVNEEIIKRPPSADTWSHYTSDEEFYWRMPIHIMDQLLYAQEHKLPLEVAERNTG
LPGDIIEKAWRHIDRIRDTTEYVRSAPPVYYLDR
>Mature_354_residues
MQIVGSSVEKTTESHVRALSKKGAGKQIDYSDVLILNELNRDIGELAKNIRGFIRDQVTGFKKKGVVLGVSGGIDSAVAL
TLCVQEFGKENVYGLLLPEKESAPSSKTLGAEICESLGVQYEEVPISPIIEALGIYDKKEQIIKKTCPEYDPKIHKTSLV
LPDFLNQGLLNVPYIRLIKDGETISRYRLKANDYLELIGLQNVKQRSRMIVQYMYAEKLNYVVCGTTNKTELVLGQFVKY
GDGGVDLEPLADCYKTQIYALAKYLEVNEEIIKRPPSADTWSHYTSDEEFYWRMPIHIMDQLLYAQEHKLPLEVAERNTG
LPGDIIEKAWRHIDRIRDTTEYVRSAPPVYYLDR

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 40143; Mature: 40143

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIVGSSVEKTTESHVRALSKKGAGKQIDYSDVLILNELNRDIGELAKNIRGFIRDQVTG
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FKKKGVVLGVSGGIDSAVALTLCVQEFGKENVYGLLLPEKESAPSSKTLGAEICESLGVQ
HHHCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCE
YEEVPISPIIEALGIYDKKEQIIKKTCPEYDPKIHKTSLVLPDFLNQGLLNVPYIRLIKD
EECCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHEECHHHHCCCCCCCCEEEEEEC
GETISRYRLKANDYLELIGLQNVKQRSRMIVQYMYAEKLNYVVCGTTNKTELVLGQFVKY
CCHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHH
GDGGVDLEPLADCYKTQIYALAKYLEVNEEIIKRPPSADTWSHYTSDEEFYWRMPIHIMD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHH
QLLYAQEHKLPLEVAERNTGLPGDIIEKAWRHIDRIRDTTEYVRSAPPVYYLDR
HHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MQIVGSSVEKTTESHVRALSKKGAGKQIDYSDVLILNELNRDIGELAKNIRGFIRDQVTG
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FKKKGVVLGVSGGIDSAVALTLCVQEFGKENVYGLLLPEKESAPSSKTLGAEICESLGVQ
HHHCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCE
YEEVPISPIIEALGIYDKKEQIIKKTCPEYDPKIHKTSLVLPDFLNQGLLNVPYIRLIKD
EECCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHEECHHHHCCCCCCCCEEEEEEC
GETISRYRLKANDYLELIGLQNVKQRSRMIVQYMYAEKLNYVVCGTTNKTELVLGQFVKY
CCHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHH
GDGGVDLEPLADCYKTQIYALAKYLEVNEEIIKRPPSADTWSHYTSDEEFYWRMPIHIMD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHH
QLLYAQEHKLPLEVAERNTGLPGDIIEKAWRHIDRIRDTTEYVRSAPPVYYLDR
HHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]