Click here to switch to the map view.

The map label for this gene is cofE [H]

Identifier: 21226539

GI number: 21226539

Start: 556588

End: 557400

Strand: Reverse

Name: cofE [H]

Synonym: MM_0437

Alternate gene names: 21226539

Gene position: 557400-556588 (Counterclockwise)

Preceding gene: 21226540

Following gene: 21226538

Centisome position: 13.61

GC content: 48.71

Gene sequence:

>813_bases
ATGAAAATGAGTCCAGAAATCACTTCCGTCCAGATGTTCGGGATTAAGACCCCTATAATTAAATCAGGGGACGACATCGT
ACAGATTATTGAAGCTTCACTTCAGGAAGCATGTCTTGTCCCGGTTGATGGGGATATTTTTGTACTGGCTGAGTCTGCTA
TTGGTACGGCAGAAAACAGGATTGTCAATCTTGCCAGCATTACCCCCGGAAAAAAGGCTCTGTCCCTCGGAGAAAAGTAC
GGGATAGATCCCAGGGAAATGGAGCTTGTGCTTCAGGAATGTGACGAGCTCTTCGGGGGTGTTCCTGGAGCCGCACTTAC
CATAACAAAAGGTATTCTCTCTCCCAATGCCGGAATAGACGCCTCGAATGCTCCTGATGGTCATGTGGTCCTGCTCCCCA
GAGACCCGAGAAAAAGTTCCGAGAACATACGGAAAAGGCTTGAGAAGCGTTACTCCTGCAGGCTGGGAGTGATTATAGGA
GACAGCAGGACACAGCCCCTGCGGCTTGGCTGCACAGGAATTGCACTTGGAGTTTCCGGTTTTATACCTGTTGAGGACGC
CAGGGGCAGTTTTGATATTTACGGAAAGCCTCTCCGCCTCACTTACAAAGCAGCGGCAGACAACCTTGTTTCTGCTGCAG
AAATCCTGATGGGAGAAGCCGGAGAAAGAGTCCCCTGTGTGCTTATCCGTGGAGCTCCTGTAAAAATGGTTGACGAATCT
CCTGAGATGCCCACGATCTCTATGGAAGGGTGTATGTATTTCGGCAACATTATTAAATGTCGAAAAGAAGACAGCGAGAA
AGGAGATCAATAA

Upstream 100 bases:

>100_bases
TGGATACATTTATCAATATCTTCCGGTATTTTACGGTTTAATTCTATTCATATTGGTTTTATTTTTTTCTAAAACCGAGA
CTAACCACAGTTTTTAAGGC

Downstream 100 bases:

>100_bases
CGAGCTGCTATGTCTCCCTTTTCGGAATAACTTTAAATACAGGCTCGAAGGACTTAGATAAGCATTATATATGCTCGAAG
ATAATATATACAAATCGAGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKMSPEITSVQMFGIKTPIIKSGDDIVQIIEASLQEACLVPVDGDIFVLAESAIGTAENRIVNLASITPGKKALSLGEKY
GIDPREMELVLQECDELFGGVPGAALTITKGILSPNAGIDASNAPDGHVVLLPRDPRKSSENIRKRLEKRYSCRLGVIIG
DSRTQPLRLGCTGIALGVSGFIPVEDARGSFDIYGKPLRLTYKAAADNLVSAAEILMGEAGERVPCVLIRGAPVKMVDES
PEMPTISMEGCMYFGNIIKCRKEDSEKGDQ

Sequences:

>Translated_270_residues
MKMSPEITSVQMFGIKTPIIKSGDDIVQIIEASLQEACLVPVDGDIFVLAESAIGTAENRIVNLASITPGKKALSLGEKY
GIDPREMELVLQECDELFGGVPGAALTITKGILSPNAGIDASNAPDGHVVLLPRDPRKSSENIRKRLEKRYSCRLGVIIG
DSRTQPLRLGCTGIALGVSGFIPVEDARGSFDIYGKPLRLTYKAAADNLVSAAEILMGEAGERVPCVLIRGAPVKMVDES
PEMPTISMEGCMYFGNIIKCRKEDSEKGDQ
>Mature_270_residues
MKMSPEITSVQMFGIKTPIIKSGDDIVQIIEASLQEACLVPVDGDIFVLAESAIGTAENRIVNLASITPGKKALSLGEKY
GIDPREMELVLQECDELFGGVPGAALTITKGILSPNAGIDASNAPDGHVVLLPRDPRKSSENIRKRLEKRYSCRLGVIIG
DSRTQPLRLGCTGIALGVSGFIPVEDARGSFDIYGKPLRLTYKAAADNLVSAAEILMGEAGERVPCVLIRGAPVKMVDES
PEMPTISMEGCMYFGNIIKCRKEDSEKGDQ

Specific function: Catalyzes the GTP-dependent successive addition of two L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8- hydroxy-5-deazariboflavin (F420-0) to form F420-0-glutamyl- glutamate (F420-2), with a gamma-linkage between the two glutamates. Cannot

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 28950; Mature: 28950

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMSPEITSVQMFGIKTPIIKSGDDIVQIIEASLQEACLVPVDGDIFVLAESAIGTAENR
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCCC
IVNLASITPGKKALSLGEKYGIDPREMELVLQECDELFGGVPGAALTITKGILSPNAGID
EEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCC
ASNAPDGHVVLLPRDPRKSSENIRKRLEKRYSCRLGVIIGDSRTQPLRLGCTGIALGVSG
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCEEECCEEEEECCCC
FIPVEDARGSFDIYGKPLRLTYKAAADNLVSAAEILMGEAGERVPCVLIRGAPVKMVDES
CEECCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCC
PEMPTISMEGCMYFGNIIKCRKEDSEKGDQ
CCCCEEECCCHHHHHHHHEECCCCCCCCCC
>Mature Secondary Structure
MKMSPEITSVQMFGIKTPIIKSGDDIVQIIEASLQEACLVPVDGDIFVLAESAIGTAENR
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCCC
IVNLASITPGKKALSLGEKYGIDPREMELVLQECDELFGGVPGAALTITKGILSPNAGID
EEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCC
ASNAPDGHVVLLPRDPRKSSENIRKRLEKRYSCRLGVIIGDSRTQPLRLGCTGIALGVSG
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCEEECCEEEEECCCC
FIPVEDARGSFDIYGKPLRLTYKAAADNLVSAAEILMGEAGERVPCVLIRGAPVKMVDES
CEECCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCC
PEMPTISMEGCMYFGNIIKCRKEDSEKGDQ
CCCCEEECCCHHHHHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]