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The map label for this gene is yitJ [H]

Identifier: 21226540

GI number: 21226540

Start: 557737

End: 558615

Strand: Reverse

Name: yitJ [H]

Synonym: MM_0438

Alternate gene names: 21226540

Gene position: 558615-557737 (Counterclockwise)

Preceding gene: 21226541

Following gene: 21226539

Centisome position: 13.64

GC content: 45.39

Gene sequence:

>879_bases
ATGCTTTTTAATTTTCGTGAAAAACTGAATTCCAGAAAATTTCTGGTTACTGCTGAAGTTTCTCCTCCCAAGGGTACAAG
ATTCTCAGCTTCTCTGGAAGATGCCAGCCAGTTAAAAGGTATTGCAGATGCTCTTAATGTTACGGATAATCAGTGTTCAA
TTATGCACATGAGTTCTCTGGCTTTTAGCAAGCTTCTGCTTGATGAAGGGCATGAGCCTATCATGCAGCTTACCTGCCGG
GACAGGAACAGAATAGGGCTCCAATCTGACCTTCTGGGAGCATATGCGCTTGGAATAAGAAACATCTGCCTTATGACCGG
AGACTATCCTACCTGTGGAGACCATCCGGGCTCGAAGCCTGTTTATGACGTTGACTCCGTGCAGCTCATAGAACTCGTGA
GAAAACTCGACACGGGCGTCGATTTTGCAGGAAACAAACTGGACGGGGGGACCTCATTTTGTGCGGGAGCAGTTTCGGGA
ATAGACCCTGAAAAGACAATGCAGCTGATAAAGCTCCAGAAAAAGGTTAAATGCGGGGCGGATTTTATCCAGACGCAGGC
TGTATACGATATAGGTATGTTCGAGGAGTTTATGGACGCAGTAAACTATCTTGAGGTGCCTGTAATTGCAGGTCTGATTC
CTCTCAAGTCTCTGGGTATGGCTGAATTTATGAATAAAAACATCTCAGGGATTCACGTGCCCGAAGAAATTATGCTTCGA
ATGAAAGATGCATCATCCCCTATGGAAGAAGGGCTTTTGATAGCTTCCGAAACCATAAAGGAGCTGACAAAACTTTCGCG
GGGAATTCACGTTATGCCAATAGGGTCCCATAGAAACACAGCAAGATTGCTTTCTCTGGCGGGAATTTCTCAGAAGTGA

Upstream 100 bases:

>100_bases
TTATACCACTTTTTATATAATAAGGGGCTTGATTTCTTTACGTCCTTATGCTCCACAGGAGCATGGATTTAAATAATTTC
ATACCTAATATTCTTATTTA

Downstream 100 bases:

>100_bases
TTAATTCAGAAAAACCGGCAAATAAAAACAGTAAAGTTAGCTGGCACTCTTTTTATGTTTATTTATTACCTGATACCTTT
TTTCTCCTTTTTTTGGCAGG

Product: methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MLFNFREKLNSRKFLVTAEVSPPKGTRFSASLEDASQLKGIADALNVTDNQCSIMHMSSLAFSKLLLDEGHEPIMQLTCR
DRNRIGLQSDLLGAYALGIRNICLMTGDYPTCGDHPGSKPVYDVDSVQLIELVRKLDTGVDFAGNKLDGGTSFCAGAVSG
IDPEKTMQLIKLQKKVKCGADFIQTQAVYDIGMFEEFMDAVNYLEVPVIAGLIPLKSLGMAEFMNKNISGIHVPEEIMLR
MKDASSPMEEGLLIASETIKELTKLSRGIHVMPIGSHRNTARLLSLAGISQK

Sequences:

>Translated_292_residues
MLFNFREKLNSRKFLVTAEVSPPKGTRFSASLEDASQLKGIADALNVTDNQCSIMHMSSLAFSKLLLDEGHEPIMQLTCR
DRNRIGLQSDLLGAYALGIRNICLMTGDYPTCGDHPGSKPVYDVDSVQLIELVRKLDTGVDFAGNKLDGGTSFCAGAVSG
IDPEKTMQLIKLQKKVKCGADFIQTQAVYDIGMFEEFMDAVNYLEVPVIAGLIPLKSLGMAEFMNKNISGIHVPEEIMLR
MKDASSPMEEGLLIASETIKELTKLSRGIHVMPIGSHRNTARLLSLAGISQK
>Mature_292_residues
MLFNFREKLNSRKFLVTAEVSPPKGTRFSASLEDASQLKGIADALNVTDNQCSIMHMSSLAFSKLLLDEGHEPIMQLTCR
DRNRIGLQSDLLGAYALGIRNICLMTGDYPTCGDHPGSKPVYDVDSVQLIELVRKLDTGVDFAGNKLDGGTSFCAGAVSG
IDPEKTMQLIKLQKKVKCGADFIQTQAVYDIGMFEEFMDAVNYLEVPVIAGLIPLKSLGMAEFMNKNISGIHVPEEIMLR
MKDASSPMEEGLLIASETIKELTKLSRGIHVMPIGSHRNTARLLSLAGISQK

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325234, Length=228, Percent_Identity=27.1929824561404, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 31928; Mature: 31928

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFNFREKLNSRKFLVTAEVSPPKGTRFSASLEDASQLKGIADALNVTDNQCSIMHMSSL
CCCCHHHHCCCCEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCEEHHHHH
AFSKLLLDEGHEPIMQLTCRDRNRIGLQSDLLGAYALGIRNICLMTGDYPTCGDHPGSKP
HHHHHHHHCCCCHHHHEEECCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCC
VYDVDSVQLIELVRKLDTGVDFAGNKLDGGTSFCAGAVSGIDPEKTMQLIKLQKKVKCGA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
DFIQTQAVYDIGMFEEFMDAVNYLEVPVIAGLIPLKSLGMAEFMNKNISGIHVPEEIMLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHH
MKDASSPMEEGLLIASETIKELTKLSRGIHVMPIGSHRNTARLLSLAGISQK
HHCCCCHHHHCEEHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MLFNFREKLNSRKFLVTAEVSPPKGTRFSASLEDASQLKGIADALNVTDNQCSIMHMSSL
CCCCHHHHCCCCEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCEEHHHHH
AFSKLLLDEGHEPIMQLTCRDRNRIGLQSDLLGAYALGIRNICLMTGDYPTCGDHPGSKP
HHHHHHHHCCCCHHHHEEECCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCC
VYDVDSVQLIELVRKLDTGVDFAGNKLDGGTSFCAGAVSGIDPEKTMQLIKLQKKVKCGA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
DFIQTQAVYDIGMFEEFMDAVNYLEVPVIAGLIPLKSLGMAEFMNKNISGIHVPEEIMLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHH
MKDASSPMEEGLLIASETIKELTKLSRGIHVMPIGSHRNTARLLSLAGISQK
HHCCCCHHHHCEEHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]