Click here to switch to the map view.

The map label for this gene is hphIAM [H]

Identifier: 21226380

GI number: 21226380

Start: 372834

End: 374105

Strand: Reverse

Name: hphIAM [H]

Synonym: MM_0278

Alternate gene names: 21226380

Gene position: 374105-372834 (Counterclockwise)

Preceding gene: 21226386

Following gene: 21226379

Centisome position: 9.13

GC content: 37.34

Gene sequence:

>1272_bases
ATGGAATCAAAATATACAGTTCTTGATATGTTTTCTGGCGCAGGAGGGCTTACAGAAGGGTTCTTTCAGCAGGGATTCAA
ATTTGTTTCTCATATAGAGAAAAATTGTCATGCTAGGAATTCTCTGGAAACCAGAGCGATTTATCATTCTCTGAAAGATT
CCAGCAACGAAAGGATTTATAGAGACTATATTTCAGGAAATCTCAACAGAGATGAATTTATTCATAAATTCAAAGAGCTT
GAACTCACTCCAGCTGGATTATTACAGGGGGAAGTAACTGAATCAAACGAAACTACTATGATAAAAGAGATTAAAGAGCA
TTTAAAAGTCATTGACAGCGATTCGGTTGATGTCGTTATAGGAGGGCCACCATGTCAGGCTTATTCTGTTGCCGGGAGAG
GCAGAAAGCCAAAAGAAATGAAAGACGATCCCCGTAACTATCTATATAGGCATTACATCTCTTTCTTAAAAAGCTTTGAA
CCAAAAATTTTCGTATTCGAAAACGTTCCGGGAATTAAAAGTGCAATAAATGGGATTATCTTCTCAAATTTACATGAAGA
ACTGGAAAAACTGGGATACAAAACAGAAGCTCATATGCTGAATGCAAAAGATTTTAGTGTACTTCAGGAAAGAAACCGGA
TAATATTTATTGGCTGGAAAAATGAGCACGATCTTTCATATCCTGACTTTTTAAAAACTCCTCTTGGCAGGTTTCATGTT
TCTTCCCTTTTGAATGACCTCCCCCCCTTGCAAGCTGGCGAAGGTACTGAAGGTCCTGCAAAATATACAAAGCCGTTTTC
ATGGTCATCTGAATACCTTCAAAGATTTAAGATTCGAGAAAAGAAAGATATACTTATTCAACACAACGCAAGAATACATA
ATCCTAGAGATCGTACCATATATAGACTTGCAATTGAAAAATGGGATAAAGAAAGAAAACGGCTCAAATATAATGAACTA
TCACCGGAACTCCAGACACACAAAAATAAAACGTCTTTTCTGGATAGATTTAAGGTGGTCAATCAGTATGGTTACTCCCA
TGCTATACTTGCTCATATATCAAAGGATGGTCATTATTTCATTCATCCAGATATTCATCAGGCAAGATCTCTGACGGTTA
GAGAAGTGGCAAGAATACAGTCATTTCCTGATAATTATAAATTCGAAGGCCCTCGAATCGCGCAGTATGCACAGATTGGT
AATGCCGTGCCTCCTCTTATGGCTAAAGGGATAGCAGTTGAAATCGAAAAGATGCTTGAAGAGATTTCATGA

Upstream 100 bases:

>100_bases
TCTCATCAACATGTTAATATATTTCAACTTCTTTTGACATTAATCGTTTTTTAGATTGGTAACAAACTTAAAAAGAAAAA
CAGTCTAGGTGAATCTCTGG

Downstream 100 bases:

>100_bases
ATACAGTTACCAGAGATAAAGAATATTTCTCAAAAATAAAAATAATAAGAACTGAACTTCTTATATGGGGAGAGGAAAAT
CTCAGAAAGTTTCCATGGAG

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.HphIA; Cytosine-specific methyltransferase HphIA; M.Hphi(C) [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MESKYTVLDMFSGAGGLTEGFFQQGFKFVSHIEKNCHARNSLETRAIYHSLKDSSNERIYRDYISGNLNRDEFIHKFKEL
ELTPAGLLQGEVTESNETTMIKEIKEHLKVIDSDSVDVVIGGPPCQAYSVAGRGRKPKEMKDDPRNYLYRHYISFLKSFE
PKIFVFENVPGIKSAINGIIFSNLHEELEKLGYKTEAHMLNAKDFSVLQERNRIIFIGWKNEHDLSYPDFLKTPLGRFHV
SSLLNDLPPLQAGEGTEGPAKYTKPFSWSSEYLQRFKIREKKDILIQHNARIHNPRDRTIYRLAIEKWDKERKRLKYNEL
SPELQTHKNKTSFLDRFKVVNQYGYSHAILAHISKDGHYFIHPDIHQARSLTVREVARIQSFPDNYKFEGPRIAQYAQIG
NAVPPLMAKGIAVEIEKMLEEIS

Sequences:

>Translated_423_residues
MESKYTVLDMFSGAGGLTEGFFQQGFKFVSHIEKNCHARNSLETRAIYHSLKDSSNERIYRDYISGNLNRDEFIHKFKEL
ELTPAGLLQGEVTESNETTMIKEIKEHLKVIDSDSVDVVIGGPPCQAYSVAGRGRKPKEMKDDPRNYLYRHYISFLKSFE
PKIFVFENVPGIKSAINGIIFSNLHEELEKLGYKTEAHMLNAKDFSVLQERNRIIFIGWKNEHDLSYPDFLKTPLGRFHV
SSLLNDLPPLQAGEGTEGPAKYTKPFSWSSEYLQRFKIREKKDILIQHNARIHNPRDRTIYRLAIEKWDKERKRLKYNEL
SPELQTHKNKTSFLDRFKVVNQYGYSHAILAHISKDGHYFIHPDIHQARSLTVREVARIQSFPDNYKFEGPRIAQYAQIG
NAVPPLMAKGIAVEIEKMLEEIS
>Mature_423_residues
MESKYTVLDMFSGAGGLTEGFFQQGFKFVSHIEKNCHARNSLETRAIYHSLKDSSNERIYRDYISGNLNRDEFIHKFKEL
ELTPAGLLQGEVTESNETTMIKEIKEHLKVIDSDSVDVVIGGPPCQAYSVAGRGRKPKEMKDDPRNYLYRHYISFLKSFE
PKIFVFENVPGIKSAINGIIFSNLHEELEKLGYKTEAHMLNAKDFSVLQERNRIIFIGWKNEHDLSYPDFLKTPLGRFHV
SSLLNDLPPLQAGEGTEGPAKYTKPFSWSSEYLQRFKIREKKDILIQHNARIHNPRDRTIYRLAIEKWDKERKRLKYNEL
SPELQTHKNKTSFLDRFKVVNQYGYSHAILAHISKDGHYFIHPDIHQARSLTVREVARIQSFPDNYKFEGPRIAQYAQIG
NAVPPLMAKGIAVEIEKMLEEIS

Specific function: This methylase protects the DNA from cleavage by the HphI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=262, Percent_Identity=24.4274809160305, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 48968; Mature: 48968

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKYTVLDMFSGAGGLTEGFFQQGFKFVSHIEKNCHARNSLETRAIYHSLKDSSNERIY
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHH
RDYISGNLNRDEFIHKFKELELTPAGLLQGEVTESNETTMIKEIKEHLKVIDSDSVDVVI
HHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
GGPPCQAYSVAGRGRKPKEMKDDPRNYLYRHYISFLKSFEPKIFVFENVPGIKSAINGII
CCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHH
FSNLHEELEKLGYKTEAHMLNAKDFSVLQERNRIIFIGWKNEHDLSYPDFLKTPLGRFHV
HHHHHHHHHHHCCCHHHHEECCHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCHHHHHH
SSLLNDLPPLQAGEGTEGPAKYTKPFSWSSEYLQRFKIREKKDILIQHNARIHNPRDRTI
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHH
YRLAIEKWDKERKRLKYNELSPELQTHKNKTSFLDRFKVVNQYGYSHAILAHISKDGHYF
HHHHHHHHHHHHHHCCHHHCCCHHHHCCCHHHHHHHHHHHHHCCCHHEEHEEECCCCCEE
IHPDIHQARSLTVREVARIQSFPDNYKFEGPRIAQYAQIGNAVPPLMAKGIAVEIEKMLE
ECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHCCHHHHHHHHHH
EIS
HCC
>Mature Secondary Structure
MESKYTVLDMFSGAGGLTEGFFQQGFKFVSHIEKNCHARNSLETRAIYHSLKDSSNERIY
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHH
RDYISGNLNRDEFIHKFKELELTPAGLLQGEVTESNETTMIKEIKEHLKVIDSDSVDVVI
HHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
GGPPCQAYSVAGRGRKPKEMKDDPRNYLYRHYISFLKSFEPKIFVFENVPGIKSAINGII
CCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHH
FSNLHEELEKLGYKTEAHMLNAKDFSVLQERNRIIFIGWKNEHDLSYPDFLKTPLGRFHV
HHHHHHHHHHHCCCHHHHEECCHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCHHHHHH
SSLLNDLPPLQAGEGTEGPAKYTKPFSWSSEYLQRFKIREKKDILIQHNARIHNPRDRTI
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHH
YRLAIEKWDKERKRLKYNELSPELQTHKNKTSFLDRFKVVNQYGYSHAILAHISKDGHYF
HHHHHHHHHHHHHHCCHHHCCCHHHHCCCHHHHHHHHHHHHHCCCHHEEHEEECCCCCEE
IHPDIHQARSLTVREVARIQSFPDNYKFEGPRIAQYAQIGNAVPPLMAKGIAVEIEKMLE
ECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHCCHHHHHHHHHH
EIS
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]