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The map label for this gene is mutY [C]

Identifier: 21226379

GI number: 21226379

Start: 372163

End: 372837

Strand: Reverse

Name: mutY [C]

Synonym: MM_0277

Alternate gene names: 21226379

Gene position: 372837-372163 (Counterclockwise)

Preceding gene: 21226380

Following gene: 21226378

Centisome position: 9.1

GC content: 35.85

Gene sequence:

>675_bases
ATGAATACAGTTACCAGAGATAAAGAATATTTCTCAAAAATAAAAATAATAAGAACTGAACTTCTTATATGGGGAGAGGA
AAATCTCAGAAAGTTTCCATGGAGAGAAACCTCTGATCCCTATAAAATTGCCGTTGCTGAAGTGATGCTTCATAGAACGA
AAGCTGATCAGGTAAAAAATATTTATGAACAATTTATTCTTAAATATCCTGATTTTGAGTCAATTGTTAAAGCTGGTCGT
GAAGCAATTAAAGCTGATCTAAAATCTCTTGGACTTTTCTGGCGTGCAGATTTACTTTACGACATGGCTGTGGAAGTTAT
GGAAAAATATGGTGGGGAACTGCCTCTGGATAGGAAAAAATTAATGACAATGCCGGGAGTAGGAAATTATATCTCAGCAG
CCATTCTATGTTTTGGCTATAACTTTCCTGAGCCAGTACTGGATACAAATACTGTCCGTGTTCTTGGAAGAATTTTTGGG
CTGAAAATTACTGATTCATCAAGGAGGAGCAAGCTTTTCTACGGGATTATGCATGATCTTGTTAATTTCTGGGATCCGAG
AACTGTTTCTTTCGCTTTAATTGATTTTGCAAACGTGGTTTGTATTCCCAGTGATAAACCCAGATGTGAAATTTGTAGTC
TTAGAGATATCTGTATCTATTACTCAAAGCTGTAA

Upstream 100 bases:

>100_bases
GCCCTCGAATCGCGCAGTATGCACAGATTGGTAATGCCGTGCCTCCTCTTATGGCTAAAGGGATAGCAGTTGAAATCGAA
AAGATGCTTGAAGAGATTTC

Downstream 100 bases:

>100_bases
GCTCCGAATTGTGATTCTTAATACTTTTCACTAATTTATTTTTGAGCAACTGTATTCTAAAATATATACTCTTGTATCTT
TCATGAAAATATAAATCTAG

Product: T/G-specific DNA glycosylase

Products: NA

Alternate protein names: Mismatch glycosylase; MIG; Thymine-DNA glycosylase [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MNTVTRDKEYFSKIKIIRTELLIWGEENLRKFPWRETSDPYKIAVAEVMLHRTKADQVKNIYEQFILKYPDFESIVKAGR
EAIKADLKSLGLFWRADLLYDMAVEVMEKYGGELPLDRKKLMTMPGVGNYISAAILCFGYNFPEPVLDTNTVRVLGRIFG
LKITDSSRRSKLFYGIMHDLVNFWDPRTVSFALIDFANVVCIPSDKPRCEICSLRDICIYYSKL

Sequences:

>Translated_224_residues
MNTVTRDKEYFSKIKIIRTELLIWGEENLRKFPWRETSDPYKIAVAEVMLHRTKADQVKNIYEQFILKYPDFESIVKAGR
EAIKADLKSLGLFWRADLLYDMAVEVMEKYGGELPLDRKKLMTMPGVGNYISAAILCFGYNFPEPVLDTNTVRVLGRIFG
LKITDSSRRSKLFYGIMHDLVNFWDPRTVSFALIDFANVVCIPSDKPRCEICSLRDICIYYSKL
>Mature_224_residues
MNTVTRDKEYFSKIKIIRTELLIWGEENLRKFPWRETSDPYKIAVAEVMLHRTKADQVKNIYEQFILKYPDFESIVKAGR
EAIKADLKSLGLFWRADLLYDMAVEVMEKYGGELPLDRKKLMTMPGVGNYISAAILCFGYNFPEPVLDTNTVRVLGRIFG
LKITDSSRRSKLFYGIMHDLVNFWDPRTVSFALIDFANVVCIPSDKPRCEICSLRDICIYYSKL

Specific function: Could act in DNA repair. Restriction methylase M.mthtI, which is encoded by this plasmid, generates 5-methylcytosine which is, especially under thermophilic conditions, subject to deamination resulting in G-T mismatches. This protein could correct these m

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298654, Length=217, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI115298652, Length=217, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI115298648, Length=217, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI190358497, Length=217, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI6912520, Length=217, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI115298650, Length=217, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI1789331, Length=203, Percent_Identity=27.0935960591133, Blast_Score=94, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 26020; Mature: 26020

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVTRDKEYFSKIKIIRTELLIWGEENLRKFPWRETSDPYKIAVAEVMLHRTKADQVKN
CCCCCCCHHHHHHHHHHHHHHEEECCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IYEQFILKYPDFESIVKAGREAIKADLKSLGLFWRADLLYDMAVEVMEKYGGELPLDRKK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LMTMPGVGNYISAAILCFGYNFPEPVLDTNTVRVLGRIFGLKITDSSRRSKLFYGIMHDL
HCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEECCCCHHHHHHHHHHHHH
VNFWDPRTVSFALIDFANVVCIPSDKPRCEICSLRDICIYYSKL
HHHCCCCHHHHHEEHHHCEEEECCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTVTRDKEYFSKIKIIRTELLIWGEENLRKFPWRETSDPYKIAVAEVMLHRTKADQVKN
CCCCCCCHHHHHHHHHHHHHHEEECCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IYEQFILKYPDFESIVKAGREAIKADLKSLGLFWRADLLYDMAVEVMEKYGGELPLDRKK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LMTMPGVGNYISAAILCFGYNFPEPVLDTNTVRVLGRIFGLKITDSSRRSKLFYGIMHDL
HCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEECCCCHHHHHHHHHHHHH
VNFWDPRTVSFALIDFANVVCIPSDKPRCEICSLRDICIYYSKL
HHHCCCCHHHHHEEHHHCEEEECCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1336177; 11786018 [H]