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The map label for this gene is ilvD1 [H]

Identifier: 21226361

GI number: 21226361

Start: 345352

End: 347013

Strand: Reverse

Name: ilvD1 [H]

Synonym: MM_0259

Alternate gene names: 21226361

Gene position: 347013-345352 (Counterclockwise)

Preceding gene: 21226364

Following gene: 21226355

Centisome position: 8.47

GC content: 50.72

Gene sequence:

>1662_bases
ATGAGAAGTGCCATTATCAAAGAGGGGCCCGAACGTGCCGCAAACCGTTCCCTTCTGAAGGCAACAGGGGTCACGGACGC
GGAAATGAAGAGGCCGTTCATAGCGGTTGTCAATTCCTGGAACGATATAATTCCTGGTCACATTCATCTGAACAAACTTG
CTGAAGCTGTAAAGGCAGGAATCAGGAACGCCGGGGGAGTTCCTTTCGAGTTCCACACCATAGGGGTCTGCGACGGGATT
GCAATGGGGCACGAGGGTATGAAATATTCTCTCCCAAGCAGGGAGGTTATAGAGGACACTATCGAGCTGATGGTCAGAGC
CCACCAGTTTGACGGCATGGTTCTTATCCCCACATGTGACAAAATCGTCCCCGGGCACCTTATGGCAGCAGGCAGGCTTG
ACATTCCTGCAATCGTTGTAACAGGAGGGCCTATGCTCCCCGGATATGTGGACGACAAATATACTGACCTTATTTCAGTC
TTTGAAGGTGTCGGCGCCTTCAGGGCAGGCAAACTTTCCGAAGCTGAGCTTGAAAGGCTTGAAAACCTTTCCTGTGCAGG
AGCAGGATCCTGTGCCGGGATGTTCACTGCAAACACAATGGCATGTATGACCGAAGCCCTCGGATTGAGTCTGCCAGGGT
GTGCAACTGCACATGCCGTTGACGCAAAAAAAGTTCGCATCGCCAAGGAATCCGGAGAGCGCATTGTTGAGCTTGTAAAA
CAGAACATAACGCCAAGGAAGATCGTCACTTACAAATCCTTTGAAAATGCCATAATGGTCGACATGGCAGTGGGAGGGAG
CACAAATACAACCCTTCACCTTCCTGCCCTTGCCCATGAATTCGGGCTTGAATTGCCTCTTGAAGCCTTTGACGAACTGA
GCAGGAAAACGCCCCACCTTATTTCTCTGAGGCCCGGAGGTCCCAATTTCATGCTCCATTTCGACAGGGCAGGCGGGGTC
GAAGCAGTTATGCAGAGGCTTTCTTCCAAACTCCACCTTGACCAGCTTACGGTTAACGGCAAAACCATCGGAGAAAACAT
CAGCGAGCTTGAAATAATCAATCCGAAACTGAATGAAGAGATCATTAAAACTCTGGAAAACCCAATTCATGCAGAAGGAG
GAATTGCAGTTCTCAAAGGCAGCCTCGCTCCCGATGGCTCGGTTGTAAAACAGGCTGCAGTTGACCCGAAAATGCGCGTA
CATACAGGACCTGCAAAAGTTTATGATTGTGAAGAAGACGCCATGAAGAGCATTCTTGCAGGAGAGGTTAAACCGGGAGA
TATTGTCGTCATCCGTTACGAAGGCCCCAAAGGCGGTCCGGGAATGAGGGAAATGCTCGCAGCCACAGCCGCAATCGCAG
GTATGGGCCTGCTGGAATCCGTAGCTCTGGTGACTGATGGGAGGTTCTCCGGAGGCACAAGGGGACCATGCATAGGGCAC
GTTTCTCCTGAAGCCAGTGAAGGAGGTCCTATAGGTCTTGTAAAGGACGGAGACCTTATCGAAATCAATATTCCGGAAAG
GATATTGAACCTGAAAGTCTCTGAAGAAGAGCTTGAAAAGCGCAGGTCTGTCTTTAATCCACCTGAAAAGAAAGTCACAG
GTTACCTTGCAAGGTACCAGCGTGCCGTCCATTCCGCAAACACGGGCGGGATAGTGGACTGA

Upstream 100 bases:

>100_bases
TTTTTTCCTATCTATACATTATCTATCTGTACATTATAATGGATTACGTTCATACTTTTCAGTGTCAAGATAACAAAGAG
AGACTAACAGGAGATTCATT

Downstream 100 bases:

>100_bases
CTTTTCAATACTGCGTTTTCAGGGAGGTAAAAACCTCCTTTTTCTCTTTTTAATTTAGCCCTCCTTTTTTTAAATAAAGG
CAGTCAATTTCAGGTAGCCC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD 1 [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MRSAIIKEGPERAANRSLLKATGVTDAEMKRPFIAVVNSWNDIIPGHIHLNKLAEAVKAGIRNAGGVPFEFHTIGVCDGI
AMGHEGMKYSLPSREVIEDTIELMVRAHQFDGMVLIPTCDKIVPGHLMAAGRLDIPAIVVTGGPMLPGYVDDKYTDLISV
FEGVGAFRAGKLSEAELERLENLSCAGAGSCAGMFTANTMACMTEALGLSLPGCATAHAVDAKKVRIAKESGERIVELVK
QNITPRKIVTYKSFENAIMVDMAVGGSTNTTLHLPALAHEFGLELPLEAFDELSRKTPHLISLRPGGPNFMLHFDRAGGV
EAVMQRLSSKLHLDQLTVNGKTIGENISELEIINPKLNEEIIKTLENPIHAEGGIAVLKGSLAPDGSVVKQAAVDPKMRV
HTGPAKVYDCEEDAMKSILAGEVKPGDIVVIRYEGPKGGPGMREMLAATAAIAGMGLLESVALVTDGRFSGGTRGPCIGH
VSPEASEGGPIGLVKDGDLIEINIPERILNLKVSEEELEKRRSVFNPPEKKVTGYLARYQRAVHSANTGGIVD

Sequences:

>Translated_553_residues
MRSAIIKEGPERAANRSLLKATGVTDAEMKRPFIAVVNSWNDIIPGHIHLNKLAEAVKAGIRNAGGVPFEFHTIGVCDGI
AMGHEGMKYSLPSREVIEDTIELMVRAHQFDGMVLIPTCDKIVPGHLMAAGRLDIPAIVVTGGPMLPGYVDDKYTDLISV
FEGVGAFRAGKLSEAELERLENLSCAGAGSCAGMFTANTMACMTEALGLSLPGCATAHAVDAKKVRIAKESGERIVELVK
QNITPRKIVTYKSFENAIMVDMAVGGSTNTTLHLPALAHEFGLELPLEAFDELSRKTPHLISLRPGGPNFMLHFDRAGGV
EAVMQRLSSKLHLDQLTVNGKTIGENISELEIINPKLNEEIIKTLENPIHAEGGIAVLKGSLAPDGSVVKQAAVDPKMRV
HTGPAKVYDCEEDAMKSILAGEVKPGDIVVIRYEGPKGGPGMREMLAATAAIAGMGLLESVALVTDGRFSGGTRGPCIGH
VSPEASEGGPIGLVKDGDLIEINIPERILNLKVSEEELEKRRSVFNPPEKKVTGYLARYQRAVHSANTGGIVD
>Mature_553_residues
MRSAIIKEGPERAANRSLLKATGVTDAEMKRPFIAVVNSWNDIIPGHIHLNKLAEAVKAGIRNAGGVPFEFHTIGVCDGI
AMGHEGMKYSLPSREVIEDTIELMVRAHQFDGMVLIPTCDKIVPGHLMAAGRLDIPAIVVTGGPMLPGYVDDKYTDLISV
FEGVGAFRAGKLSEAELERLENLSCAGAGSCAGMFTANTMACMTEALGLSLPGCATAHAVDAKKVRIAKESGERIVELVK
QNITPRKIVTYKSFENAIMVDMAVGGSTNTTLHLPALAHEFGLELPLEAFDELSRKTPHLISLRPGGPNFMLHFDRAGGV
EAVMQRLSSKLHLDQLTVNGKTIGENISELEIINPKLNEEIIKTLENPIHAEGGIAVLKGSLAPDGSVVKQAAVDPKMRV
HTGPAKVYDCEEDAMKSILAGEVKPGDIVVIRYEGPKGGPGMREMLAATAAIAGMGLLESVALVTDGRFSGGTRGPCIGH
VSPEASEGGPIGLVKDGDLIEINIPERILNLKVSEEELEKRRSVFNPPEKKVTGYLARYQRAVHSANTGGIVD

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=608, Percent_Identity=43.9144736842105, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786464, Length=500, Percent_Identity=35.6, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI2367371, Length=520, Percent_Identity=35, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1788157, Length=519, Percent_Identity=31.4065510597302, Blast_Score=223, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322476, Length=564, Percent_Identity=40.7801418439716, Blast_Score=400, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59014; Mature: 59014

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSAIIKEGPERAANRSLLKATGVTDAEMKRPFIAVVNSWNDIIPGHIHLNKLAEAVKAG
CCCCCCCCCHHHHHCCHHHHHCCCCHHHHCCCEEEEECCCCCCCCCEEEHHHHHHHHHHH
IRNAGGVPFEFHTIGVCDGIAMGHEGMKYSLPSREVIEDTIELMVRAHQFDGMVLIPTCD
HHCCCCCCEEEEEEECCCCHHCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCC
KIVPGHLMAAGRLDIPAIVVTGGPMLPGYVDDKYTDLISVFEGVGAFRAGKLSEAELERL
CCCCCCEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ENLSCAGAGSCAGMFTANTMACMTEALGLSLPGCATAHAVDAKKVRIAKESGERIVELVK
HCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHEEEEHHHHHHHHHHHH
QNITPRKIVTYKSFENAIMVDMAVGGSTNTTLHLPALAHEFGLELPLEAFDELSRKTPHL
HCCCCCEEEEEECCCCEEEEEEEECCCCCCEEECCHHHHHHCCCCCHHHHHHHHCCCCCE
ISLRPGGPNFMLHFDRAGGVEAVMQRLSSKLHLDQLTVNGKTIGENISELEIINPKLNEE
EEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHCCCCCCEEEECCCCCHH
IIKTLENPIHAEGGIAVLKGSLAPDGSVVKQAAVDPKMRVHTGPAKVYDCEEDAMKSILA
HHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCEEECHHHHHHHHHH
GEVKPGDIVVIRYEGPKGGPGMREMLAATAAIAGMGLLESVALVTDGRFSGGTRGPCIGH
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEC
VSPEASEGGPIGLVKDGDLIEINIPERILNLKVSEEELEKRRSVFNPPEKKVTGYLARYQ
CCCCCCCCCCEEEEECCCEEEEECCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
RAVHSANTGGIVD
HHHHCCCCCCCCC
>Mature Secondary Structure
MRSAIIKEGPERAANRSLLKATGVTDAEMKRPFIAVVNSWNDIIPGHIHLNKLAEAVKAG
CCCCCCCCCHHHHHCCHHHHHCCCCHHHHCCCEEEEECCCCCCCCCEEEHHHHHHHHHHH
IRNAGGVPFEFHTIGVCDGIAMGHEGMKYSLPSREVIEDTIELMVRAHQFDGMVLIPTCD
HHCCCCCCEEEEEEECCCCHHCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCC
KIVPGHLMAAGRLDIPAIVVTGGPMLPGYVDDKYTDLISVFEGVGAFRAGKLSEAELERL
CCCCCCEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ENLSCAGAGSCAGMFTANTMACMTEALGLSLPGCATAHAVDAKKVRIAKESGERIVELVK
HCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHEEEEHHHHHHHHHHHH
QNITPRKIVTYKSFENAIMVDMAVGGSTNTTLHLPALAHEFGLELPLEAFDELSRKTPHL
HCCCCCEEEEEECCCCEEEEEEEECCCCCCEEECCHHHHHHCCCCCHHHHHHHHCCCCCE
ISLRPGGPNFMLHFDRAGGVEAVMQRLSSKLHLDQLTVNGKTIGENISELEIINPKLNEE
EEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHCCCCCCEEEECCCCCHH
IIKTLENPIHAEGGIAVLKGSLAPDGSVVKQAAVDPKMRVHTGPAKVYDCEEDAMKSILA
HHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCEEECHHHHHHHHHH
GEVKPGDIVVIRYEGPKGGPGMREMLAATAAIAGMGLLESVALVTDGRFSGGTRGPCIGH
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEC
VSPEASEGGPIGLVKDGDLIEINIPERILNLKVSEEELEKRRSVFNPPEKKVTGYLARYQ
CCCCCCCCCCEEEEECCCEEEEECCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
RAVHSANTGGIVD
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]