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The map label for this gene is rfcL

Identifier: 21226355

GI number: 21226355

Start: 337562

End: 339394

Strand: Reverse

Name: rfcL

Synonym: MM_0253

Alternate gene names: 21226355

Gene position: 339394-337562 (Counterclockwise)

Preceding gene: 21226361

Following gene: 21226354

Centisome position: 8.29

GC content: 47.95

Gene sequence:

>1833_bases
TTGGTATGGTTTAAGATGATGTCGGCAATTGAATGGGCTGAAAAATACCGACCCCGGACCCTTGGAGATGTCGTGGGGAA
CAGGAAGGCAGTGCAGGATTTGAGAAAGTGGGCTGAGGAATGGCAGTCCGGGATTCCTGAGAAAAGGGCAGTGATTCTTT
ACGGGCCTGCAGGGATAGGGAAGACTTCAAGCGCTCACGCACTGGCAGGGGATATGGAATGGGAGGTTATAGAGCTCAAT
GCAAGCGACCAGAGGACTGCAGGTGTTATTGAGAAGATAGCCGGCTCAGCAGCGTCAATGAATACTTTTTTTGGGGGTAA
GCGGCTCATTATACTTGATGAAGCTGACAATCTCCACGGGACTGCAGACAGGGGCGGGATGAGGGCCATTTCCGGGATCA
TAAAATCCACGCTCCAGCCGATAATACTTATTGCAAATGATATTTACGGGTTAACTCCCACAGTCCGGAACATTTGTCTG
GAAATTAAGTTTGGGTCCGTGCAGAGCCGGTCCATGGTTCCCGCTTTGAAGAAGGTCTGTGAATCTGAGGGAGTCTCCTG
CAGCCAGGAAGCTGTCCTGCAAATTGCAGAAAATGCGGGCGGAGACTTCAGGAGCGCTATCAATGATCTTCAGGCTGCAG
CTAACGGGAAGAAGGCTCTCGAAGCTGAAGATATCAGCACTGCAGGCAGAGATGTTAAAGAGAATATCTTTAAGGCGATG
CAGAAGATCTTTAAAAGCACTGACTGCAAAAGGGCTCTTGAATCCGCATACGGGCTTGATGAGAGTCCTGAGGATCTTGT
GCACTGGATAGACGAGAACCTGCCAATCCAGTACGCGCGGAAGGATGGCGACCTCGAAGATATAAAAACGGGTTTTGGCT
ACCTTTCAAAGGCTGACATTTATCTCGGGCGCGTGAAGAAACGCCAGAACTACAGGATGTGGAGGTATGCCAGCATGCTC
ATGGTCTGCGGGGCTGCCCTTTCAAAGACAAGACCGTATCCTGGCTTTATTAAGTACCAGCAGCCTTCACTCTGGAGAAG
GCTCGGGCAGACCCGTTCCAGGCGTGATATGCGGGATAATATTGCTTCAAAGATCGGGGAGCACAGCTTTGAGTCGATGC
ATTATTCCAGGAACAACCTTTTAGGTTTTTATTCAATATTGTTAAAGGATGAGGCGTCTGCTGTTGAACTTACTGCAAAC
CTCGGGCTTGAGCTTGAAGAACTGATGTACCTTTCAGGAAGCGCAAAGGTCAGCAAAAAACTTCAGAAAATTTACGATGA
AGCCCAGAACCTTCTGGAAACGAAAAGGGATAGAACTGAAGAACAGGAATTTTTTAAGGCTCCTGCTCCTGCGGCAGATG
ATAAACAGGCAACTCTCCACTGTCCGGTTAATATTCCTGAAAAAGAAGGGAACGCTTCAGCCGAAAAACCCGAATCTCCC
GGTCCCCAGTCTCCGGAAAGAAAGCAGAAAACCCTTGACCTTGGATTTGACACACCTCAAAAGCTTCCTGAGAAAAAAAG
GTCTTCAGAAATGAAATTGCCTGAAAATCCTGAGCCTGCAGAAAATAATCTGTTCTCTCTTTCTGAGCCTCTGCCCGAAA
AGGGTCCAATTCCTGACTTCGCAGGGAAAAAGTCCCTTCCTGAACTCGAAGAAGAGAAGCCGTCTCTTTCACCGTTAAAA
AAGATGAGTCCGGCCAATAAAGAAGCTTCAAAACAGGGAGTGAAACAGGGAGCATCTGAAAAAGGTTCGCCTGCTGCAGA
TACGCAGGGAGGCATGGAAGATGGTTCGAAAAAAGCCGAACCTAAAAATCAGAAAACACTTTTTGACTTTTAA

Upstream 100 bases:

>100_bases
TTATTTATCCATGAAAGGTTTGAGGAAATTGTAAAACAGGGGGCAGGGGTCATTTTAAAAAAATTAAAACATATAAAGTT
AATAATGAGTTCTCTTTTTA

Downstream 100 bases:

>100_bases
AACACTTTTTGAGTCTTAAAACACGCTTTGACTTTTAAAACACTTATTGAGTCTTAATTTCTTTCTTAAATAATAATCAA
TTCACTCATCCATAATCAAC

Product: replication factor C large subunit

Products: NA

Alternate protein names: RFC large subunit; Clamp loader large subunit

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MVWFKMMSAIEWAEKYRPRTLGDVVGNRKAVQDLRKWAEEWQSGIPEKRAVILYGPAGIGKTSSAHALAGDMEWEVIELN
ASDQRTAGVIEKIAGSAASMNTFFGGKRLIILDEADNLHGTADRGGMRAISGIIKSTLQPIILIANDIYGLTPTVRNICL
EIKFGSVQSRSMVPALKKVCESEGVSCSQEAVLQIAENAGGDFRSAINDLQAAANGKKALEAEDISTAGRDVKENIFKAM
QKIFKSTDCKRALESAYGLDESPEDLVHWIDENLPIQYARKDGDLEDIKTGFGYLSKADIYLGRVKKRQNYRMWRYASML
MVCGAALSKTRPYPGFIKYQQPSLWRRLGQTRSRRDMRDNIASKIGEHSFESMHYSRNNLLGFYSILLKDEASAVELTAN
LGLELEELMYLSGSAKVSKKLQKIYDEAQNLLETKRDRTEEQEFFKAPAPAADDKQATLHCPVNIPEKEGNASAEKPESP
GPQSPERKQKTLDLGFDTPQKLPEKKRSSEMKLPENPEPAENNLFSLSEPLPEKGPIPDFAGKKSLPELEEEKPSLSPLK
KMSPANKEASKQGVKQGASEKGSPAADTQGGMEDGSKKAEPKNQKTLFDF

Sequences:

>Translated_610_residues
MVWFKMMSAIEWAEKYRPRTLGDVVGNRKAVQDLRKWAEEWQSGIPEKRAVILYGPAGIGKTSSAHALAGDMEWEVIELN
ASDQRTAGVIEKIAGSAASMNTFFGGKRLIILDEADNLHGTADRGGMRAISGIIKSTLQPIILIANDIYGLTPTVRNICL
EIKFGSVQSRSMVPALKKVCESEGVSCSQEAVLQIAENAGGDFRSAINDLQAAANGKKALEAEDISTAGRDVKENIFKAM
QKIFKSTDCKRALESAYGLDESPEDLVHWIDENLPIQYARKDGDLEDIKTGFGYLSKADIYLGRVKKRQNYRMWRYASML
MVCGAALSKTRPYPGFIKYQQPSLWRRLGQTRSRRDMRDNIASKIGEHSFESMHYSRNNLLGFYSILLKDEASAVELTAN
LGLELEELMYLSGSAKVSKKLQKIYDEAQNLLETKRDRTEEQEFFKAPAPAADDKQATLHCPVNIPEKEGNASAEKPESP
GPQSPERKQKTLDLGFDTPQKLPEKKRSSEMKLPENPEPAENNLFSLSEPLPEKGPIPDFAGKKSLPELEEEKPSLSPLK
KMSPANKEASKQGVKQGASEKGSPAADTQGGMEDGSKKAEPKNQKTLFDF
>Mature_610_residues
MVWFKMMSAIEWAEKYRPRTLGDVVGNRKAVQDLRKWAEEWQSGIPEKRAVILYGPAGIGKTSSAHALAGDMEWEVIELN
ASDQRTAGVIEKIAGSAASMNTFFGGKRLIILDEADNLHGTADRGGMRAISGIIKSTLQPIILIANDIYGLTPTVRNICL
EIKFGSVQSRSMVPALKKVCESEGVSCSQEAVLQIAENAGGDFRSAINDLQAAANGKKALEAEDISTAGRDVKENIFKAM
QKIFKSTDCKRALESAYGLDESPEDLVHWIDENLPIQYARKDGDLEDIKTGFGYLSKADIYLGRVKKRQNYRMWRYASML
MVCGAALSKTRPYPGFIKYQQPSLWRRLGQTRSRRDMRDNIASKIGEHSFESMHYSRNNLLGFYSILLKDEASAVELTAN
LGLELEELMYLSGSAKVSKKLQKIYDEAQNLLETKRDRTEEQEFFKAPAPAADDKQATLHCPVNIPEKEGNASAEKPESP
GPQSPERKQKTLDLGFDTPQKLPEKKRSSEMKLPENPEPAENNLFSLSEPLPEKGPIPDFAGKKSLPELEEEKPSLSPLK
KMSPANKEASKQGVKQGASEKGSPAADTQGGMEDGSKKAEPKNQKTLFDF

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcL subfamily

Homologues:

Organism=Homo sapiens, GI6677723, Length=233, Percent_Identity=33.9055793991416, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI32528306, Length=272, Percent_Identity=26.4705882352941, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI194306571, Length=233, Percent_Identity=33.4763948497854, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI194306567, Length=233, Percent_Identity=33.4763948497854, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI31563534, Length=266, Percent_Identity=29.3233082706767, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI4506491, Length=243, Percent_Identity=26.7489711934156, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI31881687, Length=243, Percent_Identity=26.7489711934156, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI27501458, Length=376, Percent_Identity=22.3404255319149, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI194306569, Length=163, Percent_Identity=33.1288343558282, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17563226, Length=248, Percent_Identity=27.0161290322581, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17541988, Length=221, Percent_Identity=32.579185520362, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17554730, Length=218, Percent_Identity=30.7339449541284, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71986063, Length=226, Percent_Identity=29.2035398230088, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17541368, Length=239, Percent_Identity=23.8493723849372, Blast_Score=83, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324791, Length=257, Percent_Identity=29.9610894941634, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324039, Length=208, Percent_Identity=33.1730769230769, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323724, Length=319, Percent_Identity=23.8244514106583, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322528, Length=229, Percent_Identity=28.3842794759825, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324478, Length=221, Percent_Identity=28.5067873303167, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17737765, Length=276, Percent_Identity=25.3623188405797, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19921076, Length=268, Percent_Identity=26.4925373134328, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859927, Length=284, Percent_Identity=30.9859154929577, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI62472391, Length=216, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17647857, Length=295, Percent_Identity=28.4745762711864, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28574716, Length=362, Percent_Identity=22.6519337016575, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFCL_METMA (Q8Q084)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_632277.1
- ProteinModelPortal:   Q8Q084
- GeneID:   1478595
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_0253
- NMPDR:   fig|192952.1.peg.253
- HOGENOM:   HBG541322
- OMA:   IANDIYG
- ProtClustDB:   PRK04195
- BioCyc:   MMAZ192952:MM0253-MONOMER
- HAMAP:   MF_01508
- InterPro:   IPR003593
- InterPro:   IPR003959
- SMART:   SM00382

Pfam domain/function: PF00004 AAA

EC number: NA

Molecular weight: Translated: 67574; Mature: 67574

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWFKMMSAIEWAEKYRPRTLGDVVGNRKAVQDLRKWAEEWQSGIPEKRAVILYGPAGIG
CHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
KTSSAHALAGDMEWEVIELNASDQRTAGVIEKIAGSAASMNTFFGGKRLIILDEADNLHG
CCCCCHHEECCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCCC
TADRGGMRAISGIIKSTLQPIILIANDIYGLTPTVRNICLEIKFGSVQSRSMVPALKKVC
CCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHEEEEEECCCCCHHHHHHHHHHH
ESEGVSCSQEAVLQIAENAGGDFRSAINDLQAAANGKKALEAEDISTAGRDVKENIFKAM
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
QKIFKSTDCKRALESAYGLDESPEDLVHWIDENLPIQYARKDGDLEDIKTGFGYLSKADI
HHHHHCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCHHHHHH
YLGRVKKRQNYRMWRYASMLMVCGAALSKTRPYPGFIKYQQPSLWRRLGQTRSRRDMRDN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHH
IASKIGEHSFESMHYSRNNLLGFYSILLKDEASAVELTANLGLELEELMYLSGSAKVSKK
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEHHHCCCCHHHHHHHCCCHHHHHH
LQKIYDEAQNLLETKRDRTEEQEFFKAPAPAADDKQATLHCPVNIPEKEGNASAEKPESP
HHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCC
GPQSPERKQKTLDLGFDTPQKLPEKKRSSEMKLPENPEPAENNLFSLSEPLPEKGPIPDF
CCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCC
AGKKSLPELEEEKPSLSPLKKMSPANKEASKQGVKQGASEKGSPAADTQGGMEDGSKKAE
CCCCCCCCHHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
PKNQKTLFDF
CCCCCCCCCC
>Mature Secondary Structure
MVWFKMMSAIEWAEKYRPRTLGDVVGNRKAVQDLRKWAEEWQSGIPEKRAVILYGPAGIG
CHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
KTSSAHALAGDMEWEVIELNASDQRTAGVIEKIAGSAASMNTFFGGKRLIILDEADNLHG
CCCCCHHEECCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCCC
TADRGGMRAISGIIKSTLQPIILIANDIYGLTPTVRNICLEIKFGSVQSRSMVPALKKVC
CCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHEEEEEECCCCCHHHHHHHHHHH
ESEGVSCSQEAVLQIAENAGGDFRSAINDLQAAANGKKALEAEDISTAGRDVKENIFKAM
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
QKIFKSTDCKRALESAYGLDESPEDLVHWIDENLPIQYARKDGDLEDIKTGFGYLSKADI
HHHHHCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCHHHHHH
YLGRVKKRQNYRMWRYASMLMVCGAALSKTRPYPGFIKYQQPSLWRRLGQTRSRRDMRDN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHH
IASKIGEHSFESMHYSRNNLLGFYSILLKDEASAVELTANLGLELEELMYLSGSAKVSKK
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEHHHCCCCHHHHHHHCCCHHHHHH
LQKIYDEAQNLLETKRDRTEEQEFFKAPAPAADDKQATLHCPVNIPEKEGNASAEKPESP
HHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCC
GPQSPERKQKTLDLGFDTPQKLPEKKRSSEMKLPENPEPAENNLFSLSEPLPEKGPIPDF
CCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCC
AGKKSLPELEEEKPSLSPLKKMSPANKEASKQGVKQGASEKGSPAADTQGGMEDGSKKAE
CCCCCCCCHHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
PKNQKTLFDF
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824