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The map label for this gene is cheB1

Identifier: 161485682

GI number: 161485682

Start: 435363

End: 436415

Strand: Reverse

Name: cheB1

Synonym: MM_0329

Alternate gene names: 161485682

Gene position: 436415-435363 (Counterclockwise)

Preceding gene: 21226432

Following gene: 21226430

Centisome position: 10.65

GC content: 44.44

Gene sequence:

>1053_bases
ATGATTCGTACTCTTATTGTTGATGATTCTGCCTTCATGCGGATGGCAATAAGAAGCATGCTTGCCAGCAGCCCGGATAT
AAAGATAGCAGGAGATGCGTGTAATGGAAAGGAGGCTGTAGATAAATCAAAATCATTGCACCCTGACATAATCATAATGG
ACGTTAACATGCCAGTCATGGACGGGCTTACAGCCGTTAAAACCATTATGAGTACGGACCCTGTCCCGATAATCATGTTC
AGTACTCTGACAGTAGAGGGCTCAAAAGAGGCTTTAGAAGCTTTGCAGCTTGGAGCAATAGATTTTGTCCCGAAATCTGA
ATCTCACCACGATGTGAATAAGATTGAGAAAGAGCTTGTTGACAAAATAAGAAATATTCACAGCTCGAACCCCAGCATAT
TAAGATTAATTAATATGAGGAAATTTAAAGGCGAGGTTGTCAGAGGGAAATGGAGCTGTGCTGGAGACTTTGCGGTCCTT
ATAGGTTCGTCTACCGGAGGTCCTTCATCTCTTGAACAGGTGATCCCAAGGCTTCCGGGTGACCTGCCTGCTCCGGTCTT
TGTTGTCCAGCATATGCCAGAAGGCAATTTTTGCAAACAACTTGCAGAGAGGCTGAATTTCCTTTCAGAGCTGGAAATAA
AGGAAGCCAGAAATAATGAAAAAGTAACTGCTGGTGTTGTTTACATAGCCCCCGGCGGCTATCACATGACTGTTAAAAAG
GCTCTGGGTGTAACCAGAATAAAGCTCATAAAAAGCCAGCCTGTTCATGCGGTAATGCCTGCAGTTGATGTGATAGCTGA
AAGCATGCTGGACGTTTACGGAAAGAACATAGTCGCTGCAATTCTTACGGGAATGGGGTTTGATGGGGCTGCCGGATTTA
AAAAGATAAGGGATGCAGGCGGCTCCACAATTGCATGCAGTGAAGATACCTGCGTTATTTTCGGAATGCCAAAAGCTGCA
ATAGCAGCCGGCGGTATTGATACGGTAAAACCCATTTTTGAGATCCCTGAAGAAATAGTAAGGATGTCGGAGGTGAAATG
CAATGGAAAGTGA

Upstream 100 bases:

>100_bases
ATAAAACTGGGGGCAAGAGGTTACATCATAAAACCTTTCCAGGCCCCCAAGGTCATTGAAGAGATTAAGAAAGTAGTCGG
TGAATGAATTCGGGGATAGT

Downstream 100 bases:

>100_bases
TATGGCAGCCTATAAAAGCGATTTCCTTCAGGAAGTATACGAGTGCCTTGATATATTCAACCAGTCTTTTGTGGACCTGG
AAAATGGGGACACTGCTGCA

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MIRTLIVDDSAFMRMAIRSMLASSPDIKIAGDACNGKEAVDKSKSLHPDIIIMDVNMPVMDGLTAVKTIMSTDPVPIIMF
STLTVEGSKEALEALQLGAIDFVPKSESHHDVNKIEKELVDKIRNIHSSNPSILRLINMRKFKGEVVRGKWSCAGDFAVL
IGSSTGGPSSLEQVIPRLPGDLPAPVFVVQHMPEGNFCKQLAERLNFLSELEIKEARNNEKVTAGVVYIAPGGYHMTVKK
ALGVTRIKLIKSQPVHAVMPAVDVIAESMLDVYGKNIVAAILTGMGFDGAAGFKKIRDAGGSTIACSEDTCVIFGMPKAA
IAAGGIDTVKPIFEIPEEIVRMSEVKCNGK

Sequences:

>Translated_350_residues
MIRTLIVDDSAFMRMAIRSMLASSPDIKIAGDACNGKEAVDKSKSLHPDIIIMDVNMPVMDGLTAVKTIMSTDPVPIIMF
STLTVEGSKEALEALQLGAIDFVPKSESHHDVNKIEKELVDKIRNIHSSNPSILRLINMRKFKGEVVRGKWSCAGDFAVL
IGSSTGGPSSLEQVIPRLPGDLPAPVFVVQHMPEGNFCKQLAERLNFLSELEIKEARNNEKVTAGVVYIAPGGYHMTVKK
ALGVTRIKLIKSQPVHAVMPAVDVIAESMLDVYGKNIVAAILTGMGFDGAAGFKKIRDAGGSTIACSEDTCVIFGMPKAA
IAAGGIDTVKPIFEIPEEIVRMSEVKCNGK
>Mature_350_residues
MIRTLIVDDSAFMRMAIRSMLASSPDIKIAGDACNGKEAVDKSKSLHPDIIIMDVNMPVMDGLTAVKTIMSTDPVPIIMF
STLTVEGSKEALEALQLGAIDFVPKSESHHDVNKIEKELVDKIRNIHSSNPSILRLINMRKFKGEVVRGKWSCAGDFAVL
IGSSTGGPSSLEQVIPRLPGDLPAPVFVVQHMPEGNFCKQLAERLNFLSELEIKEARNNEKVTAGVVYIAPGGYHMTVKK
ALGVTRIKLIKSQPVHAVMPAVDVIAESMLDVYGKNIVAAILTGMGFDGAAGFKKIRDAGGSTIACSEDTCVIFGMPKAA
IAAGGIDTVKPIFEIPEEIVRMSEVKCNGK

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=341, Percent_Identity=35.7771260997067, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI1788222, Length=134, Percent_Identity=33.5820895522388, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB1_METMA (Q8Q009)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_632353.2
- ProteinModelPortal:   Q8Q009
- SMR:   Q8Q009
- GeneID:   1478671
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_0329
- NMPDR:   fig|192952.1.peg.329
- HOGENOM:   HBG705324
- OMA:   CLVYGMA
- ProtClustDB:   PRK00742
- BioCyc:   MMAZ192952:MM0329-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 37645; Mature: 37645

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 171-171 ACT_SITE 198-198 ACT_SITE 295-295

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTLIVDDSAFMRMAIRSMLASSPDIKIAGDACNGKEAVDKSKSLHPDIIIMDVNMPVM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCEEEEECCCCHH
DGLTAVKTIMSTDPVPIIMFSTLTVEGSKEALEALQLGAIDFVPKSESHHDVNKIEKELV
HHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHH
DKIRNIHSSNPSILRLINMRKFKGEVVRGKWSCAGDFAVLIGSSTGGPSSLEQVIPRLPG
HHHHHHCCCCCCEEHHHHHHHHCCCEEECEECCCCCEEEEEECCCCCHHHHHHHHHHCCC
DLPAPVFVVQHMPEGNFCKQLAERLNFLSELEIKEARNNEKVTAGVVYIAPGGYHMTVKK
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEHHH
ALGVTRIKLIKSQPVHAVMPAVDVIAESMLDVYGKNIVAAILTGMGFDGAAGFKKIRDAG
HHCHHHEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
GSTIACSEDTCVIFGMPKAAIAAGGIDTVKPIFEIPEEIVRMSEVKCNGK
CCEEEECCCCEEEEECCHHHHHCCCCHHHHHHHHCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIRTLIVDDSAFMRMAIRSMLASSPDIKIAGDACNGKEAVDKSKSLHPDIIIMDVNMPVM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCEEEEECCCCHH
DGLTAVKTIMSTDPVPIIMFSTLTVEGSKEALEALQLGAIDFVPKSESHHDVNKIEKELV
HHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHH
DKIRNIHSSNPSILRLINMRKFKGEVVRGKWSCAGDFAVLIGSSTGGPSSLEQVIPRLPG
HHHHHHCCCCCCEEHHHHHHHHCCCEEECEECCCCCEEEEEECCCCCHHHHHHHHHHCCC
DLPAPVFVVQHMPEGNFCKQLAERLNFLSELEIKEARNNEKVTAGVVYIAPGGYHMTVKK
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEHHH
ALGVTRIKLIKSQPVHAVMPAVDVIAESMLDVYGKNIVAAILTGMGFDGAAGFKKIRDAG
HHCHHHEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
GSTIACSEDTCVIFGMPKAAIAAGGIDTVKPIFEIPEEIVRMSEVKCNGK
CCEEEECCCCEEEEECCHHHHHCCCCHHHHHHHHCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824