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The map label for this gene is trpB2 [H]

Identifier: 161485681

GI number: 161485681

Start: 443958

End: 445256

Strand: Reverse

Name: trpB2 [H]

Synonym: MM_0337

Alternate gene names: 161485681

Gene position: 445256-443958 (Counterclockwise)

Preceding gene: 21226440

Following gene: 21226435

Centisome position: 10.87

GC content: 49.58

Gene sequence:

>1299_bases
ATGGAGCAGACAAAAATTATCCTTGACGAAAATGAAATGCCGAAGAGATGGTACAACGTCCTTTCAGACCTGCCGTCTCC
AATAGACCCGCCTCTGGACCCAAGAACCTGGCAGCCTATAAACCCTGAGGCTCTTGAGCCTATTTTTGCAAAGGAACTGA
TCAGGCAGGAAATGAGCAGTGACAGGTACATCGATATCCCTGCAGAAATACTCGATGTTTACAGGCTCTGGAGGCCAAGC
CCCCTCTTCAGGGCTCACCAGCTTGAAAAGGACCTCAAGACCCCTGCAAAAATATATTATAAGTATGAAGGGGTCAGCCC
CGCAGGCAGCCACAAGACAAATACCTCCATTGCCCAGGCCTACTACAATATGAAAGAAGGCACGGAACGGCTCACAACCG
AGACCGGAGCAGGACAGTGGGGAAGTGCACTTTCTCTTGCCTGCAACTACTTTGACCTCGAATGCAAGGTCTATATGGTT
CGTTCAAGTTATTACCAGAAACCTTACAGAAAATCCCTTATGACTCTCTGGGGAGGAAATGTCGTGCCTTCCCCGAGTCC
TGATACGGAGTTTGGAAGAAAAATCCTGAAGGAGCAGCCTGACACGCCCGGGAGCCTCGGGATTGCAATCAGTGAGGCTG
TAGAGGACGCAATAGCCCATGATAATACAAAATACACGCTTGGAAGCGTGCTGAACCATGTTGTTCTCCATCAGACCGTG
ATAGGAGCTGAATGTAAAAAACAGCTTGAACAGGTCGAAGAGTATCCTGATGTGGTTATAGGGTGCTGCGGAGGAGGGAG
CAACCTCGGAGGGATCGGACTCGAATTCATAAAAGACAGGCTTGAAGGAAAGCACAATGCAAGAGTGGTCGCAGTCGAGC
CTTCAGCATGTCCTTCCCTGACAAAAGGAGAATACAGATATGACTTCGGAGACACTGCCGAGATGACGCCTCTTCTCAAG
ATGTACACTCTCGGACACAAGCACATACCACCTGCCATCCATGCAGGAGGACTCCGTTATCACGGCGACTCTCCCATTAT
AAGCAAACTCTGTGCCGAAGGGCTCATGGAAGCGGTTTCTTATGGCCAGAAAGAGGTCTTCGATGCAGCTGTCCAGTTTG
CCAGGACGGAAGGAATAGTGCCTGCACCTGAATCTTCCCATGCAATACGCTGTGCGATTGATGAAGCCCTTGAAGCTAAG
CAGAAAGGAGAAGAAAAGGTAATTCTCTTCAACCTTAGCGGGCACGGGCACTTTGACATGGCTTCCTACGACAAATATTT
CAGCGGAGAACTTGAGTGA

Upstream 100 bases:

>100_bases
AAGTATAATGGTGGAGCTTATTGAATATAGATGAGAAACAGATAACACTTAAATACACCAAGGTACGATTTTGTACAGAA
ACGTTACGCAGGAGATTTTC

Downstream 100 bases:

>100_bases
TCTGCATATATCAGGAAAACATCTGATAAAAGTAATTGGATAAAAGTAATGGTGCCAAAAAAACATAGCCTGAAACATGA
ACCCGAACGATGCCCGGGTT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MEQTKIILDENEMPKRWYNVLSDLPSPIDPPLDPRTWQPINPEALEPIFAKELIRQEMSSDRYIDIPAEILDVYRLWRPS
PLFRAHQLEKDLKTPAKIYYKYEGVSPAGSHKTNTSIAQAYYNMKEGTERLTTETGAGQWGSALSLACNYFDLECKVYMV
RSSYYQKPYRKSLMTLWGGNVVPSPSPDTEFGRKILKEQPDTPGSLGIAISEAVEDAIAHDNTKYTLGSVLNHVVLHQTV
IGAECKKQLEQVEEYPDVVIGCCGGGSNLGGIGLEFIKDRLEGKHNARVVAVEPSACPSLTKGEYRYDFGDTAEMTPLLK
MYTLGHKHIPPAIHAGGLRYHGDSPIISKLCAEGLMEAVSYGQKEVFDAAVQFARTEGIVPAPESSHAIRCAIDEALEAK
QKGEEKVILFNLSGHGHFDMASYDKYFSGELE

Sequences:

>Translated_432_residues
MEQTKIILDENEMPKRWYNVLSDLPSPIDPPLDPRTWQPINPEALEPIFAKELIRQEMSSDRYIDIPAEILDVYRLWRPS
PLFRAHQLEKDLKTPAKIYYKYEGVSPAGSHKTNTSIAQAYYNMKEGTERLTTETGAGQWGSALSLACNYFDLECKVYMV
RSSYYQKPYRKSLMTLWGGNVVPSPSPDTEFGRKILKEQPDTPGSLGIAISEAVEDAIAHDNTKYTLGSVLNHVVLHQTV
IGAECKKQLEQVEEYPDVVIGCCGGGSNLGGIGLEFIKDRLEGKHNARVVAVEPSACPSLTKGEYRYDFGDTAEMTPLLK
MYTLGHKHIPPAIHAGGLRYHGDSPIISKLCAEGLMEAVSYGQKEVFDAAVQFARTEGIVPAPESSHAIRCAIDEALEAK
QKGEEKVILFNLSGHGHFDMASYDKYFSGELE
>Mature_432_residues
MEQTKIILDENEMPKRWYNVLSDLPSPIDPPLDPRTWQPINPEALEPIFAKELIRQEMSSDRYIDIPAEILDVYRLWRPS
PLFRAHQLEKDLKTPAKIYYKYEGVSPAGSHKTNTSIAQAYYNMKEGTERLTTETGAGQWGSALSLACNYFDLECKVYMV
RSSYYQKPYRKSLMTLWGGNVVPSPSPDTEFGRKILKEQPDTPGSLGIAISEAVEDAIAHDNTKYTLGSVLNHVVLHQTV
IGAECKKQLEQVEEYPDVVIGCCGGGSNLGGIGLEFIKDRLEGKHNARVVAVEPSACPSLTKGEYRYDFGDTAEMTPLLK
MYTLGHKHIPPAIHAGGLRYHGDSPIISKLCAEGLMEAVSYGQKEVFDAAVQFARTEGIVPAPESSHAIRCAIDEALEAK
QKGEEKVILFNLSGHGHFDMASYDKYFSGELE

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=397, Percent_Identity=29.2191435768262, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321412, Length=398, Percent_Identity=28.391959798995, Blast_Score=110, Evalue=5e-25,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 48095; Mature: 48095

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQTKIILDENEMPKRWYNVLSDLPSPIDPPLDPRTWQPINPEALEPIFAKELIRQEMSS
CCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
DRYIDIPAEILDVYRLWRPSPLFRAHQLEKDLKTPAKIYYKYEGVSPAGSHKTNTSIAQA
CCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHH
YYNMKEGTERLTTETGAGQWGSALSLACNYFDLECKVYMVRSSYYQKPYRKSLMTLWGGN
HHHHHHHHHHHHCCCCCCCCCCHHHHHEEEEEEEEEEEEEHHHHHCCHHHHHHHHHHCCC
VVPSPSPDTEFGRKILKEQPDTPGSLGIAISEAVEDAIAHDNTKYTLGSVLNHVVLHQTV
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
IGAECKKQLEQVEEYPDVVIGCCGGGSNLGGIGLEFIKDRLEGKHNARVVAVEPSACPSL
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCC
TKGEYRYDFGDTAEMTPLLKMYTLGHKHIPPAIHAGGLRYHGDSPIISKLCAEGLMEAVS
CCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCHHCCCEEECCCCHHHHHHHHHHHHHHHH
YGQKEVFDAAVQFARTEGIVPAPESSHAIRCAIDEALEAKQKGEEKVILFNLSGHGHFDM
CCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEEECCCCCCCH
ASYDKYFSGELE
HHHHHHHCCCCC
>Mature Secondary Structure
MEQTKIILDENEMPKRWYNVLSDLPSPIDPPLDPRTWQPINPEALEPIFAKELIRQEMSS
CCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
DRYIDIPAEILDVYRLWRPSPLFRAHQLEKDLKTPAKIYYKYEGVSPAGSHKTNTSIAQA
CCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHH
YYNMKEGTERLTTETGAGQWGSALSLACNYFDLECKVYMVRSSYYQKPYRKSLMTLWGGN
HHHHHHHHHHHHCCCCCCCCCCHHHHHEEEEEEEEEEEEEHHHHHCCHHHHHHHHHHCCC
VVPSPSPDTEFGRKILKEQPDTPGSLGIAISEAVEDAIAHDNTKYTLGSVLNHVVLHQTV
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
IGAECKKQLEQVEEYPDVVIGCCGGGSNLGGIGLEFIKDRLEGKHNARVVAVEPSACPSL
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCC
TKGEYRYDFGDTAEMTPLLKMYTLGHKHIPPAIHAGGLRYHGDSPIISKLCAEGLMEAVS
CCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCHHCCCEEECCCCHHHHHHHHHHHHHHHH
YGQKEVFDAAVQFARTEGIVPAPESSHAIRCAIDEALEAKQKGEEKVILFNLSGHGHFDM
CCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEEECCCCCCCH
ASYDKYFSGELE
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]