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The map label for this gene is thiD [C]

Identifier: 21226440

GI number: 21226440

Start: 445717

End: 447075

Strand: Reverse

Name: thiD [C]

Synonym: MM_0338

Alternate gene names: 21226440

Gene position: 447075-445717 (Counterclockwise)

Preceding gene: 21226444

Following gene: 161485681

Centisome position: 10.91

GC content: 48.2

Gene sequence:

>1359_bases
ATGAGGAATAAAATGACAGAAAAAACCCCTATGTTTAAAACTCCTGTTGTGATGACTATCGCTGGTTCTGATTCAGGAGG
GGGAGCAGGAATTGCTGCTGACATCAAGACCTTTGCAGCATTCGGAGTCCACGGGACCTGCGCCATTACTTCCGTAACCG
CCCAGAATACTACAGGGGTTCTTGAAACATTTGATCTTGCTCCTGAAGCCGTTGCGAGCCAGATAGAGGCTGTCTGTACC
GACATGGATGTTAAGTGGGCAAAAACCGGAATGCTTGCCTCTTCAGAGATCATAAAAGAGGTTGCAAAGCAGGTAAAAAA
ACATAATCTCTCCCTCGTGCTCGACCCTGTAATGGCTGCTGAAGCCGGAGGAGACCTGCTGAAAAAAGAAGCCCTTTCAG
TCCTTATTAAAGAACTGTTACCTCTCTGCAAGGTTACAACTCCAAACGCCTCAGAGGCGGGCGCACTTGCAGGTGTCACT
GTCAAGACCCATGAAGATGCAAAACTTGCGGCAAGAAAAATTGCAGACCTTGGGGTTGAATCCGTTATTGTTACGGGAGG
GCATATCGACGCGACTGACCTCCTTTATGAACCTGTGTCCGGCACCTTTACACGCATTCCGGGCACTTTTGTAAAAGGAG
GGACACATGGGTCTGGATGCACTTATTCCGCGTCAATGGCTGCCTGCCTTGCCCGGGGTGACAGCACGGAAACCGCTGCC
AGAAAAGCAAAGACGTTTGTGGAACAGGCGATTTTGAGGAGCGTGCCTGTAGGAAAGGGAGTGGGACCTGTAAATCCTCT
GGCAAATACACTTGAAGAAAAAGAGCGGTACCTGGCTCTTCAGGACGTAAAAGAAGCGGTTTCAATCCTCACATACAGCC
CTGATTTTGCAAAACTAATCCCTGAGGTTGGCTGTAATATAGGGATGGCAATCCCATGTGCCAGGACTTATAATGAAATA
GCTGCTGTCGAAGGCAGGATAATCAGGTTCAGGGAAAAGGCAATTCCCGTCGGATACGTTAATTTCGGAGCCAGCCAGCA
TGTTGCCAGGGTTATTTTAGCAGCTCTCCGCGAGAATCCTGAAATGAGGGCTGCCATGAATGTGAAATACTCGGAAGAAA
TTCTTGAAGCATGCAGAGAAATAAATCCTGGAATCTCTTCTTTTGACAGGGCTGAAGATCCTGGGGATGCCAGCACCATG
GAATGGGGAACATCGGAGGCAATTAGAAAATACGGAGGAATGCCTGAAGTAATCTATGATAAAGGAGCAATAGGAAAAGA
ACCCATGGTCAGGCTGCTTGGTAAAAGCGCTTCAGAGGTCGCAAAGCTTGCCGTGGAACTTGCCAGGAGAGTAAAATAA

Upstream 100 bases:

>100_bases
GTGTAATTCTGACAAAAATGAGAAGTGCAGTATAAATGCCGTATAAGTGCCTTATATTTAGATACACCAGCATAATAAGC
CATAAAAAATAAAAATCGAA

Downstream 100 bases:

>100_bases
CACCCGTTGAGCATAACGCCAGCTGAAACCAGGATTTTTTATTCCCAATTTTTCTTATTTTAAATATTTTTTCAGATATA
TCCAGTTTTGATATATTTAT

Product: hydroxymethylpyrimidine kinase

Products: ADP; 4-amino-2-methyl-5-diphosphomethylpyrimidine

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MRNKMTEKTPMFKTPVVMTIAGSDSGGGAGIAADIKTFAAFGVHGTCAITSVTAQNTTGVLETFDLAPEAVASQIEAVCT
DMDVKWAKTGMLASSEIIKEVAKQVKKHNLSLVLDPVMAAEAGGDLLKKEALSVLIKELLPLCKVTTPNASEAGALAGVT
VKTHEDAKLAARKIADLGVESVIVTGGHIDATDLLYEPVSGTFTRIPGTFVKGGTHGSGCTYSASMAACLARGDSTETAA
RKAKTFVEQAILRSVPVGKGVGPVNPLANTLEEKERYLALQDVKEAVSILTYSPDFAKLIPEVGCNIGMAIPCARTYNEI
AAVEGRIIRFREKAIPVGYVNFGASQHVARVILAALRENPEMRAAMNVKYSEEILEACREINPGISSFDRAEDPGDASTM
EWGTSEAIRKYGGMPEVIYDKGAIGKEPMVRLLGKSASEVAKLAVELARRVK

Sequences:

>Translated_452_residues
MRNKMTEKTPMFKTPVVMTIAGSDSGGGAGIAADIKTFAAFGVHGTCAITSVTAQNTTGVLETFDLAPEAVASQIEAVCT
DMDVKWAKTGMLASSEIIKEVAKQVKKHNLSLVLDPVMAAEAGGDLLKKEALSVLIKELLPLCKVTTPNASEAGALAGVT
VKTHEDAKLAARKIADLGVESVIVTGGHIDATDLLYEPVSGTFTRIPGTFVKGGTHGSGCTYSASMAACLARGDSTETAA
RKAKTFVEQAILRSVPVGKGVGPVNPLANTLEEKERYLALQDVKEAVSILTYSPDFAKLIPEVGCNIGMAIPCARTYNEI
AAVEGRIIRFREKAIPVGYVNFGASQHVARVILAALRENPEMRAAMNVKYSEEILEACREINPGISSFDRAEDPGDASTM
EWGTSEAIRKYGGMPEVIYDKGAIGKEPMVRLLGKSASEVAKLAVELARRVK
>Mature_452_residues
MRNKMTEKTPMFKTPVVMTIAGSDSGGGAGIAADIKTFAAFGVHGTCAITSVTAQNTTGVLETFDLAPEAVASQIEAVCT
DMDVKWAKTGMLASSEIIKEVAKQVKKHNLSLVLDPVMAAEAGGDLLKKEALSVLIKELLPLCKVTTPNASEAGALAGVT
VKTHEDAKLAARKIADLGVESVIVTGGHIDATDLLYEPVSGTFTRIPGTFVKGGTHGSGCTYSASMAACLARGDSTETAA
RKAKTFVEQAILRSVPVGKGVGPVNPLANTLEEKERYLALQDVKEAVSILTYSPDFAKLIPEVGCNIGMAIPCARTYNEI
AAVEGRIIRFREKAIPVGYVNFGASQHVARVILAALRENPEMRAAMNVKYSEEILEACREINPGISSFDRAEDPGDASTM
EWGTSEAIRKYGGMPEVIYDKGAIGKEPMVRLLGKSASEVAKLAVELARRVK

Specific function: Catalyzes The Phosphorylation Of Hmp-P To Hmp-Pp. [C]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788420, Length=255, Percent_Identity=36.4705882352941, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6325378, Length=270, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324517, Length=271, Percent_Identity=30.9963099630996, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324997, Length=274, Percent_Identity=31.021897810219, Blast_Score=106, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR013749
- InterPro:   IPR019293 [H]

Pfam domain/function: PF10120 MethylPyrKinase; PF08543 Phos_pyr_kin [H]

EC number: 2.7.4.7; 2.7.1.49

Molecular weight: Translated: 47846; Mature: 47846

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKMTEKTPMFKTPVVMTIAGSDSGGGAGIAADIKTFAAFGVHGTCAITSVTAQNTTGV
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCH
LETFDLAPEAVASQIEAVCTDMDVKWAKTGMLASSEIIKEVAKQVKKHNLSLVLDPVMAA
HHHHHCCHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECHHHHH
EAGGDLLKKEALSVLIKELLPLCKVTTPNASEAGALAGVTVKTHEDAKLAARKIADLGVE
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCC
SVIVTGGHIDATDLLYEPVSGTFTRIPGTFVKGGTHGSGCTYSASMAACLARGDSTETAA
EEEEECCCCCHHHHHHCCCCCCEEECCCCEECCCCCCCCCCCHHHHHHHHHCCCCHHHHH
RKAKTFVEQAILRSVPVGKGVGPVNPLANTLEEKERYLALQDVKEAVSILTYSPDFAKLI
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
PEVGCNIGMAIPCARTYNEIAAVEGRIIRFREKAIPVGYVNFGASQHVARVILAALRENP
HHHCCCCCCCCCCHHHHHHHHHHCCHHEEEHHHCCCEEEECCCCHHHHHHHHHHHHHCCC
EMRAAMNVKYSEEILEACREINPGISSFDRAEDPGDASTMEWGTSEAIRKYGGMPEVIYD
CHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEC
KGAIGKEPMVRLLGKSASEVAKLAVELARRVK
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRNKMTEKTPMFKTPVVMTIAGSDSGGGAGIAADIKTFAAFGVHGTCAITSVTAQNTTGV
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCH
LETFDLAPEAVASQIEAVCTDMDVKWAKTGMLASSEIIKEVAKQVKKHNLSLVLDPVMAA
HHHHHCCHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECHHHHH
EAGGDLLKKEALSVLIKELLPLCKVTTPNASEAGALAGVTVKTHEDAKLAARKIADLGVE
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCC
SVIVTGGHIDATDLLYEPVSGTFTRIPGTFVKGGTHGSGCTYSASMAACLARGDSTETAA
EEEEECCCCCHHHHHHCCCCCCEEECCCCEECCCCCCCCCCCHHHHHHHHHCCCCHHHHH
RKAKTFVEQAILRSVPVGKGVGPVNPLANTLEEKERYLALQDVKEAVSILTYSPDFAKLI
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
PEVGCNIGMAIPCARTYNEIAAVEGRIIRFREKAIPVGYVNFGASQHVARVILAALRENP
HHHCCCCCCCCCCHHHHHHHHHHCCHHEEEHHHCCCEEEECCCCHHHHHHHHHHHHHCCC
EMRAAMNVKYSEEILEACREINPGISSFDRAEDPGDASTMEWGTSEAIRKYGGMPEVIYD
CHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEC
KGAIGKEPMVRLLGKSASEVAKLAVELARRVK
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 4-amino-2-methyl-5-phosphomethylpyrimidine

Specific reaction: ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]