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The map label for this gene is iadA [H]

Identifier: 18311338

GI number: 18311338

Start: 2714487

End: 2715656

Strand: Reverse

Name: iadA [H]

Synonym: CPE2356

Alternate gene names: 18311338

Gene position: 2715656-2714487 (Counterclockwise)

Preceding gene: 18311339

Following gene: 18311337

Centisome position: 89.58

GC content: 31.62

Gene sequence:

>1170_bases
TTGTTAAAAGTAATAAAAAATATTGAGGTTTATGACCCAGAGTATTTAGGGAAAAAGGATATTGTTATATTAAGTGATAA
GATTGAAGGATTATATGATTCTGTGAATATTCCTAAAGATTTTATAAACATACAAGTAATTGATGGAGAAGGGATGATAG
CTGTACCAGGCTTTATAGATTGTCATGTCCATATAATTGGTGGAGGTGGTGAAGGTGGTTTTAGAACCAGAACTCCAGAA
TTACAATTAAGTAGTATAATAAAAGGTGGAATAACTACCCTAGTAGGGTGTATAGGAACTGATGGTGTTTGTAGAGATAT
GAAAGGATTAGTGGCAAAAGCTCATGCTCTTTGTGAAGAGGGAGTAACTTGTTACTGTTATACTGGGTCTTATGATGTAC
CTGTAAATACCATAACTAAAACCATAAAAAGTGACTTATTATTAATAGATAAAGTTATAGGTGTAGGGGAAATAGCATTA
TCAGACCATAGAAGTTCTCAACCAACATACGAGCAATTCGTCAATATAGTAGCTCAAGCAAGAGTTGGAGGACTTTTATC
AGGAAAGGCTGGAATAGTAAATGTTCATTTAGGTAATGGGAAAAGAATGATTGAATATATAACTAGACTTATAAATGAAA
CAGAGATTCCAGCAAATCAAATAATTCCTACTCATATGGGAAGAAGTAGAGAGCTTTTTGAAGCAGGAATAAATCATGCT
AAGGCTGGAGGCCAAATTGATTTAACCACATCTTCAGATCCTCAACATTTAGAAGAAGGAGAAGTAAGAGCTGGTGAAGG
ACTTAAAAAGCTTTTAGATGCTGGAGTAGATATAGAAAAAATAACTTTTTCTTCAGATGGTAATGGAAGTATGCCATTAT
TTGATGAAAATGGAGAGCTTAAAGGTTTAGGAATCTGTTCTGTTGAAAGTCTTTTTAATGAAGTAAAGGAAGCTATAAAG
CTTGGAGTACCTTTAGAAAAAGCAATAAGAGTTATAACTAGTAATGTTGCCAATGTTTTAAAACTATCTCATAAAGGATT
TATATCAACAGGAAAAGATGCAGATATAATTTTATTAGATAAGAAAAATTTAGACATAAATTCTGTTATATCTAGAGGTA
ATATTTTAATGGATAATAAAAAATTATTAGTAAAAGGCACCTTTGAATAA

Upstream 100 bases:

>100_bases
GCCTTTTTTTTATAAAAAAATAAGTTTTTATAAAATTAAATTATTTATTATTGAGTTTTAGGTAAAAATAATAATAATTT
AAATAAAGGAGGTGTATTTT

Downstream 100 bases:

>100_bases
CAAAATTATAAAAATTAGATAATAATTATTATTTGTAACATTGACTTAATTTTTTAAATATAATATAATTGTAAATAATA
AAGTACCCCTTAGGGGTATA

Product: isoaspartyl dipeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MLKVIKNIEVYDPEYLGKKDIVILSDKIEGLYDSVNIPKDFINIQVIDGEGMIAVPGFIDCHVHIIGGGGEGGFRTRTPE
LQLSSIIKGGITTLVGCIGTDGVCRDMKGLVAKAHALCEEGVTCYCYTGSYDVPVNTITKTIKSDLLLIDKVIGVGEIAL
SDHRSSQPTYEQFVNIVAQARVGGLLSGKAGIVNVHLGNGKRMIEYITRLINETEIPANQIIPTHMGRSRELFEAGINHA
KAGGQIDLTTSSDPQHLEEGEVRAGEGLKKLLDAGVDIEKITFSSDGNGSMPLFDENGELKGLGICSVESLFNEVKEAIK
LGVPLEKAIRVITSNVANVLKLSHKGFISTGKDADIILLDKKNLDINSVISRGNILMDNKKLLVKGTFE

Sequences:

>Translated_389_residues
MLKVIKNIEVYDPEYLGKKDIVILSDKIEGLYDSVNIPKDFINIQVIDGEGMIAVPGFIDCHVHIIGGGGEGGFRTRTPE
LQLSSIIKGGITTLVGCIGTDGVCRDMKGLVAKAHALCEEGVTCYCYTGSYDVPVNTITKTIKSDLLLIDKVIGVGEIAL
SDHRSSQPTYEQFVNIVAQARVGGLLSGKAGIVNVHLGNGKRMIEYITRLINETEIPANQIIPTHMGRSRELFEAGINHA
KAGGQIDLTTSSDPQHLEEGEVRAGEGLKKLLDAGVDIEKITFSSDGNGSMPLFDENGELKGLGICSVESLFNEVKEAIK
LGVPLEKAIRVITSNVANVLKLSHKGFISTGKDADIILLDKKNLDINSVISRGNILMDNKKLLVKGTFE
>Mature_389_residues
MLKVIKNIEVYDPEYLGKKDIVILSDKIEGLYDSVNIPKDFINIQVIDGEGMIAVPGFIDCHVHIIGGGGEGGFRTRTPE
LQLSSIIKGGITTLVGCIGTDGVCRDMKGLVAKAHALCEEGVTCYCYTGSYDVPVNTITKTIKSDLLLIDKVIGVGEIAL
SDHRSSQPTYEQFVNIVAQARVGGLLSGKAGIVNVHLGNGKRMIEYITRLINETEIPANQIIPTHMGRSRELFEAGINHA
KAGGQIDLTTSSDPQHLEEGEVRAGEGLKKLLDAGVDIEKITFSSDGNGSMPLFDENGELKGLGICSVESLFNEVKEAIK
LGVPLEKAIRVITSNVANVLKLSHKGFISTGKDADIILLDKKNLDINSVISRGNILMDNKKLLVKGTFE

Specific function: Catalyzes the hydrolytic cleavage of a subset of L- isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation. The best substrate for the enzyme reported thus far is iso-Asp-Leu [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M38 family [H]

Homologues:

Organism=Escherichia coli, GI1790784, Length=390, Percent_Identity=40.5128205128205, Blast_Score=300, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059
- InterPro:   IPR010229 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.4.19.- [C]

Molecular weight: Translated: 41911; Mature: 41911

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVIKNIEVYDPEYLGKKDIVILSDKIEGLYDSVNIPKDFINIQVIDGEGMIAVPGFID
CCEEECCCEEECHHHCCCCCEEEEECCCCHHHHCCCCCCHHEEEEEECCCCEEECCCEEE
CHVHIIGGGGEGGFRTRTPELQLSSIIKGGITTLVGCIGTDGVCRDMKGLVAKAHALCEE
EEEEEEECCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GVTCYCYTGSYDVPVNTITKTIKSDLLLIDKVIGVGEIALSDHRSSQPTYEQFVNIVAQA
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHH
RVGGLLSGKAGIVNVHLGNGKRMIEYITRLINETEIPANQIIPTHMGRSRELFEAGINHA
HHCCEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHCCCCC
KAGGQIDLTTSSDPQHLEEGEVRAGEGLKKLLDAGVDIEKITFSSDGNGSMPLFDENGEL
CCCCEEEEECCCCCCHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCCCE
KGLGICSVESLFNEVKEAIKLGVPLEKAIRVITSNVANVLKLSHKGFISTGKDADIILLD
EEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEE
KKNLDINSVISRGNILMDNKKLLVKGTFE
CCCCCHHHHHCCCCEEECCCEEEEEECCC
>Mature Secondary Structure
MLKVIKNIEVYDPEYLGKKDIVILSDKIEGLYDSVNIPKDFINIQVIDGEGMIAVPGFID
CCEEECCCEEECHHHCCCCCEEEEECCCCHHHHCCCCCCHHEEEEEECCCCEEECCCEEE
CHVHIIGGGGEGGFRTRTPELQLSSIIKGGITTLVGCIGTDGVCRDMKGLVAKAHALCEE
EEEEEEECCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GVTCYCYTGSYDVPVNTITKTIKSDLLLIDKVIGVGEIALSDHRSSQPTYEQFVNIVAQA
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHH
RVGGLLSGKAGIVNVHLGNGKRMIEYITRLINETEIPANQIIPTHMGRSRELFEAGINHA
HHCCEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHCCCCC
KAGGQIDLTTSSDPQHLEEGEVRAGEGLKKLLDAGVDIEKITFSSDGNGSMPLFDENGEL
CCCCEEEEECCCCCCHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCCCE
KGLGICSVESLFNEVKEAIKLGVPLEKAIRVITSNVANVLKLSHKGFISTGKDADIILLD
EEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEE
KKNLDINSVISRGNILMDNKKLLVKGTFE
CCCCCHHHHHCCCCEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7876157; 7610040; 9278503 [H]