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The map label for this gene is ptsI [H]

Identifier: 18311339

GI number: 18311339

Start: 2715821

End: 2717440

Strand: Reverse

Name: ptsI [H]

Synonym: CPE2357

Alternate gene names: 18311339

Gene position: 2717440-2715821 (Counterclockwise)

Preceding gene: 18311341

Following gene: 18311338

Centisome position: 89.64

GC content: 33.83

Gene sequence:

>1620_bases
ATGAAAAAAGGTATTGCTGCTTCAAAAGGATATGCAATAGGAACTGTATTTATACAAGAACATGAGGAAATAATAATATC
TGATGCGAAGGTTTCAGATATAGCAGCTGAAAAAGAAAAATTATCTAAAGCTTTAGCTCAATCAAAAGAGCAATTAGAAG
CAATAAAAGAAAAAACAGCTAATGAAATTGGAGAACATGAAGCTCAAGTTTTTGAAGCTCATTTAATGTTATTAGATGAT
GTAGAGTTTACTGGTCAAATGGAAATGACTATAGAAAATGACCAATTAAACGCTATGAAAGCTGTTCAAAATGTTACAGA
TACTTTCGTTATGATATTTGATTCTATGGATGACCCTTACATGAGAGAAAGGGCAGCAGATATAAAAGACGTTTCTAAGA
GAATAATAGCTAACCTAGCTGGCAAGGGTGGAAACGGAATGGAAAATGTAGGAGCTAACACTGTTGTTGTAGCTCATGAC
TTAACACCTTCAGATACTGCTCAATTAGATAGAAGTAAAGTTATTGGTTTCTTAACTAATATCGGGGGAAGAACTTCTCA
CTCAGCTATAATGGCTAGAACTTTAGAAATACCAGCTGTTGTTGGATTAGGTGATATAACAACTTCAGTTAAAAATGGGG
ACACTGTAATAGTTGATGGTATTGAGGGTGTAGCTATAATCAACCCAGATGAAGCTACTATAAATGAATATAAAGCTAGA
TTAGAAAAATTTAAAGCAGAACAAGAAGAATTAAAGAAATTAATAGATGTTAAAACAACTACTAAATCAGGTAGAAGAAT
AGAGGTTTGTGGAAACATAGGTAAACCAGAAGATATAGATCAAGTTTTAGCAAATGGTGGAGACGGAGTTGGACTATTTA
GAACTGAGTTCTTATACATGGATAGAGATGAAGCTCCAACTGAAGATGAACAATTTGAAGCATACAAATATGTTTTAGAA
AAAGCAGATGGTAAGCAAGTTGTTATCAGAACATTAGATATCGGTGGAGATAAAACTCTTCCATACTTACCATTACCAGA
AGAGATGAATCCATTCTTAGGATACAGAGCTATAAGATTATGCTTAGACAGAAAAGATATCTTTAGAGTTCAAATAAGAG
CTTTATTAAGAGCTTCTGTTTATGGAAATCTTGCAGTAATGTTCCCAATGATTTCAGGATTAGAAGAATTCCAACAAGCT
AAAGCATTTGTTGAAGAATGCAAAGCTGAGTTAAAAGCAGAAGGTATAGCATACTCAGATTCAATTCAATGGGGTATCAT
GGTTGAAATCCCAGCTGCAGCAGTTTATGCTGATGAATTAGCTAAGCATGTTGATTTCTTCTCAATAGGAACTAACGATT
TAATCCAATATACATTAGCTGCAGATAGAATGAGTGAAAAGGTATCATACCTTTACAATCCAATGCATCCAGCTGTATTA
AGATTAATCAAAATGACAATAGATGGAGCTCACAAACATGGTAAGTGGGTAGGAATGTGTGGAGAGATGGCAGGAGATGA
AAGAGCTATACCAACATTAGTTGAATATGGTTTAGATGAATTCTCAATGAGTGCTACATCAATCCTAACTGCTAAGAAAA
TAATAATGGAACAAGAATAG

Upstream 100 bases:

>100_bases
TTATATATAAGCAGTGAATTTGAAAAGTGAATTTAGTTGAATTGTGATTGATATTTAATGTTGTATTTAGTTGAGTACGA
ACTTAAAGGAGGACTACATA

Downstream 100 bases:

>100_bases
TCAATAATATTAAAAATTAATATAGAACACTCAAAACCCTAAGTTTTGGCACGAATTAATTCGTGCCTTTTTTTTATAAA
AAAATAAGTTTTTATAAAAT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MKKGIAASKGYAIGTVFIQEHEEIIISDAKVSDIAAEKEKLSKALAQSKEQLEAIKEKTANEIGEHEAQVFEAHLMLLDD
VEFTGQMEMTIENDQLNAMKAVQNVTDTFVMIFDSMDDPYMRERAADIKDVSKRIIANLAGKGGNGMENVGANTVVVAHD
LTPSDTAQLDRSKVIGFLTNIGGRTSHSAIMARTLEIPAVVGLGDITTSVKNGDTVIVDGIEGVAIINPDEATINEYKAR
LEKFKAEQEELKKLIDVKTTTKSGRRIEVCGNIGKPEDIDQVLANGGDGVGLFRTEFLYMDRDEAPTEDEQFEAYKYVLE
KADGKQVVIRTLDIGGDKTLPYLPLPEEMNPFLGYRAIRLCLDRKDIFRVQIRALLRASVYGNLAVMFPMISGLEEFQQA
KAFVEECKAELKAEGIAYSDSIQWGIMVEIPAAAVYADELAKHVDFFSIGTNDLIQYTLAADRMSEKVSYLYNPMHPAVL
RLIKMTIDGAHKHGKWVGMCGEMAGDERAIPTLVEYGLDEFSMSATSILTAKKIIMEQE

Sequences:

>Translated_539_residues
MKKGIAASKGYAIGTVFIQEHEEIIISDAKVSDIAAEKEKLSKALAQSKEQLEAIKEKTANEIGEHEAQVFEAHLMLLDD
VEFTGQMEMTIENDQLNAMKAVQNVTDTFVMIFDSMDDPYMRERAADIKDVSKRIIANLAGKGGNGMENVGANTVVVAHD
LTPSDTAQLDRSKVIGFLTNIGGRTSHSAIMARTLEIPAVVGLGDITTSVKNGDTVIVDGIEGVAIINPDEATINEYKAR
LEKFKAEQEELKKLIDVKTTTKSGRRIEVCGNIGKPEDIDQVLANGGDGVGLFRTEFLYMDRDEAPTEDEQFEAYKYVLE
KADGKQVVIRTLDIGGDKTLPYLPLPEEMNPFLGYRAIRLCLDRKDIFRVQIRALLRASVYGNLAVMFPMISGLEEFQQA
KAFVEECKAELKAEGIAYSDSIQWGIMVEIPAAAVYADELAKHVDFFSIGTNDLIQYTLAADRMSEKVSYLYNPMHPAVL
RLIKMTIDGAHKHGKWVGMCGEMAGDERAIPTLVEYGLDEFSMSATSILTAKKIIMEQE
>Mature_539_residues
MKKGIAASKGYAIGTVFIQEHEEIIISDAKVSDIAAEKEKLSKALAQSKEQLEAIKEKTANEIGEHEAQVFEAHLMLLDD
VEFTGQMEMTIENDQLNAMKAVQNVTDTFVMIFDSMDDPYMRERAADIKDVSKRIIANLAGKGGNGMENVGANTVVVAHD
LTPSDTAQLDRSKVIGFLTNIGGRTSHSAIMARTLEIPAVVGLGDITTSVKNGDTVIVDGIEGVAIINPDEATINEYKAR
LEKFKAEQEELKKLIDVKTTTKSGRRIEVCGNIGKPEDIDQVLANGGDGVGLFRTEFLYMDRDEAPTEDEQFEAYKYVLE
KADGKQVVIRTLDIGGDKTLPYLPLPEEMNPFLGYRAIRLCLDRKDIFRVQIRALLRASVYGNLAVMFPMISGLEEFQQA
KAFVEECKAELKAEGIAYSDSIQWGIMVEIPAAAVYADELAKHVDFFSIGTNDLIQYTLAADRMSEKVSYLYNPMHPAVL
RLIKMTIDGAHKHGKWVGMCGEMAGDERAIPTLVEYGLDEFSMSATSILTAKKIIMEQE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=537, Percent_Identity=48.6033519553073, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1788726, Length=537, Percent_Identity=37.8026070763501, Blast_Score=352, Evalue=4e-98,
Organism=Escherichia coli, GI48994992, Length=464, Percent_Identity=37.7155172413793, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1789193, Length=540, Percent_Identity=34.6296296296296, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1787994, Length=440, Percent_Identity=30.9090909090909, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI226510935, Length=152, Percent_Identity=26.9736842105263, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 59514; Mature: 59514

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGIAASKGYAIGTVFIQEHEEIIISDAKVSDIAAEKEKLSKALAQSKEQLEAIKEKTA
CCCCCCCCCCCEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEIGEHEAQVFEAHLMLLDDVEFTGQMEMTIENDQLNAMKAVQNVTDTFVMIFDSMDDPY
HHHHHHHHHHHHHHHHHEECCCCCCEEEEEEECCHHHHHHHHHHHHHHEEEEECCCCCHH
MRERAADIKDVSKRIIANLAGKGGNGMENVGANTVVVAHDLTPSDTAQLDRSKVIGFLTN
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
IGGRTSHSAIMARTLEIPAVVGLGDITTSVKNGDTVIVDGIEGVAIINPDEATINEYKAR
CCCCCCCHHHHHHHCCCCEEEECCHHHHCCCCCCEEEEECCCEEEEECCCHHHHHHHHHH
LEKFKAEQEELKKLIDVKTTTKSGRRIEVCGNIGKPEDIDQVLANGGDGVGLFRTEFLYM
HHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEEEE
DRDEAPTEDEQFEAYKYVLEKADGKQVVIRTLDIGGDKTLPYLPLPEEMNPFLGYRAIRL
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHHHHHHHH
CLDRKDIFRVQIRALLRASVYGNLAVMFPMISGLEEFQQAKAFVEECKAELKAEGIAYSD
HHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECC
SIQWGIMVEIPAAAVYADELAKHVDFFSIGTNDLIQYTLAADRMSEKVSYLYNPMHPAVL
CCCEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RLIKMTIDGAHKHGKWVGMCGEMAGDERAIPTLVEYGLDEFSMSATSILTAKKIIMEQE
HHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKGIAASKGYAIGTVFIQEHEEIIISDAKVSDIAAEKEKLSKALAQSKEQLEAIKEKTA
CCCCCCCCCCCEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEIGEHEAQVFEAHLMLLDDVEFTGQMEMTIENDQLNAMKAVQNVTDTFVMIFDSMDDPY
HHHHHHHHHHHHHHHHHEECCCCCCEEEEEEECCHHHHHHHHHHHHHHEEEEECCCCCHH
MRERAADIKDVSKRIIANLAGKGGNGMENVGANTVVVAHDLTPSDTAQLDRSKVIGFLTN
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
IGGRTSHSAIMARTLEIPAVVGLGDITTSVKNGDTVIVDGIEGVAIINPDEATINEYKAR
CCCCCCCHHHHHHHCCCCEEEECCHHHHCCCCCCEEEEECCCEEEEECCCHHHHHHHHHH
LEKFKAEQEELKKLIDVKTTTKSGRRIEVCGNIGKPEDIDQVLANGGDGVGLFRTEFLYM
HHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEEEE
DRDEAPTEDEQFEAYKYVLEKADGKQVVIRTLDIGGDKTLPYLPLPEEMNPFLGYRAIRL
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHHHHHHHH
CLDRKDIFRVQIRALLRASVYGNLAVMFPMISGLEEFQQAKAFVEECKAELKAEGIAYSD
HHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECC
SIQWGIMVEIPAAAVYADELAKHVDFFSIGTNDLIQYTLAADRMSEKVSYLYNPMHPAVL
CCCEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RLIKMTIDGAHKHGKWVGMCGEMAGDERAIPTLVEYGLDEFSMSATSILTAKKIIMEQE
HHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]