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The map label for this gene is pduE [H]

Identifier: 18309913

GI number: 18309913

Start: 1130044

End: 1130469

Strand: Direct

Name: pduE [H]

Synonym: CPE0931

Alternate gene names: 18309913

Gene position: 1130044-1130469 (Clockwise)

Preceding gene: 18309912

Following gene: 18309914

Centisome position: 37.28

GC content: 31.22

Gene sequence:

>426_bases
ATGGAAAATAAGAGAATGACTGCTGCAGATTATCCTTTAACTTCTAAGAGAAAAGGAGATATAAAAACTCCTACAGGAAA
AGCTTTAGAAGATATAACTTTAGAAAAAGTTTTAAGTGGAGAAATCAATGCAGATGATATAAGAATTTCACCAGAAACTT
TAGAAATGCAAGCTCAAATAGCTGAAAGCATGAACAGAGATGCTATAGCTAGAAACTTTAGAAGAGCTGCGGAGCTTATA
AGAGTTCCAGACGATAGAATATTAGAGATGTACAATGCTTTAAGACCATATCGTTCAACAAAAGAAGATTTATTCAAAAT
AGCTGATGAACTTGAAACAAAATATGATGCTAAAGTTAATGCTGATTTTGTTAGAGAAGCAGCTGAAGTATATGAAACTA
GAAATAAATTAAGAATAGAAGAGTAA

Upstream 100 bases:

>100_bases
AAGGCAGCTTTATTACACATAAAAGAAACTAAACACGTTGAACCAGGTAAAAAGCCAGTTCAATTAGAAGTTAAGTTTTA
ATTTAGAAAGGATTGAAATC

Downstream 100 bases:

>100_bases
AATCAGAGGTGAAAAGATGAAAATCATAGCAGGAATTGATATAGGAAACTCTTCTACAGAAACTGCTTTAGGTAAGGTTT
ATGAAAATAATGTTGAATTT

Product: coenzyme B12-dependent glycerol dehydrogenase small subunit

Products: NA

Alternate protein names: Propanediol utilization protein pduE [H]

Number of amino acids: Translated: 141; Mature: 141

Protein sequence:

>141_residues
MENKRMTAADYPLTSKRKGDIKTPTGKALEDITLEKVLSGEINADDIRISPETLEMQAQIAESMNRDAIARNFRRAAELI
RVPDDRILEMYNALRPYRSTKEDLFKIADELETKYDAKVNADFVREAAEVYETRNKLRIEE

Sequences:

>Translated_141_residues
MENKRMTAADYPLTSKRKGDIKTPTGKALEDITLEKVLSGEINADDIRISPETLEMQAQIAESMNRDAIARNFRRAAELI
RVPDDRILEMYNALRPYRSTKEDLFKIADELETKYDAKVNADFVREAAEVYETRNKLRIEE
>Mature_141_residues
MENKRMTAADYPLTSKRKGDIKTPTGKALEDITLEKVLSGEINADDIRISPETLEMQAQIAESMNRDAIARNFRRAAELI
RVPDDRILEMYNALRPYRSTKEDLFKIADELETKYDAKVNADFVREAAEVYETRNKLRIEE

Specific function: Part of the pduCDE complex that catalyzes the dehydration of 1,2-propanediol to propionaldehyde. Is required for S.typhimurium growth on 1,2-propanediol as the sole carbon and energy source [H]

COG id: COG4910

COG function: function code Q; Propanediol dehydratase, small subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diol/glycerol dehydratase small subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003207 [H]

Pfam domain/function: PF02287 Dehydratase_SU [H]

EC number: =4.2.1.28 [H]

Molecular weight: Translated: 16284; Mature: 16284

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKRMTAADYPLTSKRKGDIKTPTGKALEDITLEKVLSGEINADDIRISPETLEMQAQI
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHH
AESMNRDAIARNFRRAAELIRVPDDRILEMYNALRPYRSTKEDLFKIADELETKYDAKVN
HHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
ADFVREAAEVYETRNKLRIEE
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MENKRMTAADYPLTSKRKGDIKTPTGKALEDITLEKVLSGEINADDIRISPETLEMQAQI
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHH
AESMNRDAIARNFRRAAELIRVPDDRILEMYNALRPYRSTKEDLFKIADELETKYDAKVN
HHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
ADFVREAAEVYETRNKLRIEE
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9352910; 10498708; 11677609 [H]