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The map label for this gene is pduD [H]

Identifier: 18309912

GI number: 18309912

Start: 1129452

End: 1130024

Strand: Direct

Name: pduD [H]

Synonym: CPE0930

Alternate gene names: 18309912

Gene position: 1129452-1130024 (Clockwise)

Preceding gene: 18309911

Following gene: 18309913

Centisome position: 37.26

GC content: 31.41

Gene sequence:

>573_bases
ATGACAATGGAAGAAAGAACTTTTATACCAGAAATAACAGTTGAAGAAGTTGGAGAAGCTAAGGTTGGTTTAAGATCAGA
TGAAGTTGTTATAGGATTAGCACCTGCATTTTTAAAATATCAAAATAAAACAATAGTAGATGTTCCTCATACTGAAACTT
TACTTGAAATTATAGCAGGTATTGAAGAAGAAGGATTACATGCAAGAGTAGTTAGAATTTTAAGAACATCTGATGTATCT
TTTATAGCTCATGATGCAGCTTGTTTAAGTGGATCAGGAATAGGTATAGGAATACAATCAAAAGGTACTACAGTAATACA
TCAAAAAGATTTATTACCATTAAATAACTTAGAATTATTCTCACAAGCTCCGCTTTTAACTACAGAAACATATAGATTAA
TCGGAAAAAATGCTGCTAAATATGCTAAAGGTGAGTCACCAACACCAGTACCAGTAAAAAATGACCAAATGGTTAGACCT
AAATTCATGGCAAAGGCAGCTTTATTACACATAAAAGAAACTAAACACGTTGAACCAGGTAAAAAGCCAGTTCAATTAGA
AGTTAAGTTTTAA

Upstream 100 bases:

>100_bases
ATGGGACCTGGAACAGGATATAGACTAGATGGTCAAAGATGGGAAGAAATCAAAAAAGTTCCTACAGTAATGAGACCAGA
GGATATAGAGTAGGGGGAGA

Downstream 100 bases:

>100_bases
TTTAGAAAGGATTGAAATCATGGAAAATAAGAGAATGACTGCTGCAGATTATCCTTTAACTTCTAAGAGAAAAGGAGATA
TAAAAACTCCTACAGGAAAA

Product: coenzyme B12-dependent glycerol dehydrogenase medium subunit

Products: NA

Alternate protein names: Propanediol utilization protein pduD [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MTMEERTFIPEITVEEVGEAKVGLRSDEVVIGLAPAFLKYQNKTIVDVPHTETLLEIIAGIEEEGLHARVVRILRTSDVS
FIAHDAACLSGSGIGIGIQSKGTTVIHQKDLLPLNNLELFSQAPLLTTETYRLIGKNAAKYAKGESPTPVPVKNDQMVRP
KFMAKAALLHIKETKHVEPGKKPVQLEVKF

Sequences:

>Translated_190_residues
MTMEERTFIPEITVEEVGEAKVGLRSDEVVIGLAPAFLKYQNKTIVDVPHTETLLEIIAGIEEEGLHARVVRILRTSDVS
FIAHDAACLSGSGIGIGIQSKGTTVIHQKDLLPLNNLELFSQAPLLTTETYRLIGKNAAKYAKGESPTPVPVKNDQMVRP
KFMAKAALLHIKETKHVEPGKKPVQLEVKF
>Mature_189_residues
TMEERTFIPEITVEEVGEAKVGLRSDEVVIGLAPAFLKYQNKTIVDVPHTETLLEIIAGIEEEGLHARVVRILRTSDVSF
IAHDAACLSGSGIGIGIQSKGTTVIHQKDLLPLNNLELFSQAPLLTTETYRLIGKNAAKYAKGESPTPVPVKNDQMVRPK
FMAKAALLHIKETKHVEPGKKPVQLEVKF

Specific function: Part of the pduCDE complex that catalyzes the dehydration of 1,2-propanediol to propionaldehyde. Is required for S.typhimurium growth on 1,2-propanediol as the sole carbon and energy source [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diol/glycerol dehydratase medium subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010254
- InterPro:   IPR003208 [H]

Pfam domain/function: PF02288 Dehydratase_MU [H]

EC number: =4.2.1.28 [H]

Molecular weight: Translated: 20861; Mature: 20729

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMEERTFIPEITVEEVGEAKVGLRSDEVVIGLAPAFLKYQNKTIVDVPHTETLLEIIAG
CCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEECHHHHEECCCEEEECCCHHHHHHHHHC
IEEEGLHARVVRILRTSDVSFIAHDAACLSGSGIGIGIQSKGTTVIHQKDLLPLNNLELF
CCCCCHHHHHHHHHHHCCCEEEEECHHHCCCCCEEEEECCCCCEEEEECCCCCCCCCHHH
SQAPLLTTETYRLIGKNAAKYAKGESPTPVPVKNDQMVRPKFMAKAALLHIKETKHVEPG
HCCCCEEHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCCCCCCC
KKPVQLEVKF
CCCEEEEEEC
>Mature Secondary Structure 
TMEERTFIPEITVEEVGEAKVGLRSDEVVIGLAPAFLKYQNKTIVDVPHTETLLEIIAG
CCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEECHHHHEECCCEEEECCCHHHHHHHHHC
IEEEGLHARVVRILRTSDVSFIAHDAACLSGSGIGIGIQSKGTTVIHQKDLLPLNNLELF
CCCCCHHHHHHHHHHHCCCEEEEECHHHCCCCCEEEEECCCCCEEEEECCCCCCCCCHHH
SQAPLLTTETYRLIGKNAAKYAKGESPTPVPVKNDQMVRPKFMAKAALLHIKETKHVEPG
HCCCCEEHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCCCCCCC
KKPVQLEVKF
CCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9352910; 10498708; 11677609 [H]