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The map label for this gene is tgt [H]

Identifier: 17547432

GI number: 17547432

Start: 2918884

End: 2920035

Strand: Direct

Name: tgt [H]

Synonym: RSc2713

Alternate gene names: 17547432

Gene position: 2918884-2920035 (Clockwise)

Preceding gene: 17547431

Following gene: 17547433

Centisome position: 78.54

GC content: 67.71

Gene sequence:

>1152_bases
ATGCTGAATTTCGACCTCCTCACCACCGATGGCCTCGCCCGGCGCGGCCGCATGACCCTCAACCACGGCGTGGTGGAAAC
GCCCATCTTCATGCCGGTGGGCACCTACGGCGCGGTCAAGGCGATGTCGCCGGTGGAGCTCAAGGACATCGGCGCGCAGA
TCATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCCGCGCTGGAGGTGATCGACGCGCACAAGGGCCTGCACGGCTTC
GTCGGCTGGGACAAGCCGATGCTGACCGACTCCGGCGGCTTCCAGGTGTTTTCGCTGGGCGATCTGCGCAAGATCACCGA
AGAAGGCGTGCATTTCGCCTCGCCCATCAACGGCGACCGGCTGTTCCTGTCGCCCGAGATCTCGATGCAGATCCAGCGCC
GGCTGAACTCCGACATCGTCATGCAATTCGACGAATGCACGCCGTACCAGATCGACGGCCGGCCGGCCACCGAGGCGGAA
GCAGCCGCCTCCATGCGCATGAGCCTGCGCTGGGCGCAGCGCTCGCGCAACGAGTTCGAGCGGGAGCGCAATCCCAACGC
CCTGTTCGGCATCGTCCAGGGCGGCATGTTCGAGCCCCTGCGCGATGAATCGCTGGCCGGCCTGCAGGCCATCGACGCCG
ACGCGGGCGGCCAGGGCTTCGGCGGCTACGCCATCGGCGGGCTGTCGGTGGGCGAGCCCAAGGAAGACATGATACGCGTG
CTGCAGCACGTGGCGCCGCGCCTGCCCGCCAACAAGCCGCACTACCTGATGGGCGTGGGCACGCCGGAAGACCTGGTGGC
GGGCGTGGCCGCCGGCATCGACATGTTCGACTGCGTGATGCCGACCCGCAACGCGCGCAACGGCTGGCTGTTCACCCGCT
TCGGCGACATCAAGATCAAGAACGCGGTGCACCGCAACGATCCGCGCCCGCTGGACGAGACCTGCGGCTGCTACACCTGC
CGCAACTTCTCGCGCGCCTACCTGCACCACCTGCAGCGCGTCGGCGAGATCCTGGGCGCCCGCCTGAACACCATCCACAA
CCTCCACTACTACCTGGAGCTGATGGCCGACATGCGCACGGCGATCGAATCGCACAGCTTCGCCGTCTTCCGGGCCCGCT
TCGCCGCCGACCGAGCGCGCGGGGCACTCTGA

Upstream 100 bases:

>100_bases
GCCACGCCATCGCGCAGCGCTACCGCTTCTTCAGCTACGGCGATGCCATGTTCCTGACGCGCGCCGAGCCTGATACCCAG
TCCGCTTCCCCCGACCCCTC

Downstream 100 bases:

>100_bases
GCGCACTTGACAAACCCGCCGATGCACCCATTTTCCGCTGGACCGCGATGGTAGAATATCCGGTCCGATTTTTGACCGAT
TGACGAACCATTACGGAGTT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MLNFDLLTTDGLARRGRMTLNHGVVETPIFMPVGTYGAVKAMSPVELKDIGAQIILGNTFHLWLRPALEVIDAHKGLHGF
VGWDKPMLTDSGGFQVFSLGDLRKITEEGVHFASPINGDRLFLSPEISMQIQRRLNSDIVMQFDECTPYQIDGRPATEAE
AAASMRMSLRWAQRSRNEFERERNPNALFGIVQGGMFEPLRDESLAGLQAIDADAGGQGFGGYAIGGLSVGEPKEDMIRV
LQHVAPRLPANKPHYLMGVGTPEDLVAGVAAGIDMFDCVMPTRNARNGWLFTRFGDIKIKNAVHRNDPRPLDETCGCYTC
RNFSRAYLHHLQRVGEILGARLNTIHNLHYYLELMADMRTAIESHSFAVFRARFAADRARGAL

Sequences:

>Translated_383_residues
MLNFDLLTTDGLARRGRMTLNHGVVETPIFMPVGTYGAVKAMSPVELKDIGAQIILGNTFHLWLRPALEVIDAHKGLHGF
VGWDKPMLTDSGGFQVFSLGDLRKITEEGVHFASPINGDRLFLSPEISMQIQRRLNSDIVMQFDECTPYQIDGRPATEAE
AAASMRMSLRWAQRSRNEFERERNPNALFGIVQGGMFEPLRDESLAGLQAIDADAGGQGFGGYAIGGLSVGEPKEDMIRV
LQHVAPRLPANKPHYLMGVGTPEDLVAGVAAGIDMFDCVMPTRNARNGWLFTRFGDIKIKNAVHRNDPRPLDETCGCYTC
RNFSRAYLHHLQRVGEILGARLNTIHNLHYYLELMADMRTAIESHSFAVFRARFAADRARGAL
>Mature_383_residues
MLNFDLLTTDGLARRGRMTLNHGVVETPIFMPVGTYGAVKAMSPVELKDIGAQIILGNTFHLWLRPALEVIDAHKGLHGF
VGWDKPMLTDSGGFQVFSLGDLRKITEEGVHFASPINGDRLFLSPEISMQIQRRLNSDIVMQFDECTPYQIDGRPATEAE
AAASMRMSLRWAQRSRNEFERERNPNALFGIVQGGMFEPLRDESLAGLQAIDADAGGQGFGGYAIGGLSVGEPKEDMIRV
LQHVAPRLPANKPHYLMGVGTPEDLVAGVAAGIDMFDCVMPTRNARNGWLFTRFGDIKIKNAVHRNDPRPLDETCGCYTC
RNFSRAYLHHLQRVGEILGARLNTIHNLHYYLELMADMRTAIESHSFAVFRARFAADRARGAL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=362, Percent_Identity=43.9226519337017, Blast_Score=288, Evalue=8e-78,
Organism=Escherichia coli, GI1786607, Length=379, Percent_Identity=63.3245382585752, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17544680, Length=378, Percent_Identity=41.005291005291, Blast_Score=277, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI25146502, Length=161, Percent_Identity=26.7080745341615, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920510, Length=364, Percent_Identity=43.6813186813187, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI21355311, Length=193, Percent_Identity=27.4611398963731, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42525; Mature: 42525

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFDLLTTDGLARRGRMTLNHGVVETPIFMPVGTYGAVKAMSPVELKDIGAQIILGNTF
CCCEEEECCCCHHHCCCEEECCCCCCCCEEEECCCCCHHHCCCCCCHHHCCCEEEECCCH
HLWLRPALEVIDAHKGLHGFVGWDKPMLTDSGGFQVFSLGDLRKITEEGVHFASPINGDR
HHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCE
LFLSPEISMQIQRRLNSDIVMQFDECTPYQIDGRPATEAEAAASMRMSLRWAQRSRNEFE
EEECCCHHHHHHHHCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RERNPNALFGIVQGGMFEPLRDESLAGLQAIDADAGGQGFGGYAIGGLSVGEPKEDMIRV
HHCCCCEEEEEECCCCCCCCCCCHHCCCEEECCCCCCCCCCCEEECCCCCCCCHHHHHHH
LQHVAPRLPANKPHYLMGVGTPEDLVAGVAAGIDMFDCVMPTRNARNGWLFTRFGDIKIK
HHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCEEEE
NAVHRNDPRPLDETCGCYTCRNFSRAYLHHLQRVGEILGARLNTIHNLHYYLELMADMRT
CCHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIESHSFAVFRARFAADRARGAL
HHHCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNFDLLTTDGLARRGRMTLNHGVVETPIFMPVGTYGAVKAMSPVELKDIGAQIILGNTF
CCCEEEECCCCHHHCCCEEECCCCCCCCEEEECCCCCHHHCCCCCCHHHCCCEEEECCCH
HLWLRPALEVIDAHKGLHGFVGWDKPMLTDSGGFQVFSLGDLRKITEEGVHFASPINGDR
HHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCE
LFLSPEISMQIQRRLNSDIVMQFDECTPYQIDGRPATEAEAAASMRMSLRWAQRSRNEFE
EEECCCHHHHHHHHCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RERNPNALFGIVQGGMFEPLRDESLAGLQAIDADAGGQGFGGYAIGGLSVGEPKEDMIRV
HHCCCCEEEEEECCCCCCCCCCCHHCCCEEECCCCCCCCCCCEEECCCCCCCCHHHHHHH
LQHVAPRLPANKPHYLMGVGTPEDLVAGVAAGIDMFDCVMPTRNARNGWLFTRFGDIKIK
HHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCEEEE
NAVHRNDPRPLDETCGCYTCRNFSRAYLHHLQRVGEILGARLNTIHNLHYYLELMADMRT
CCHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIESHSFAVFRARFAADRARGAL
HHHCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA