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The map label for this gene is queA [H]

Identifier: 17547431

GI number: 17547431

Start: 2917802

End: 2918890

Strand: Direct

Name: queA [H]

Synonym: RSc2712

Alternate gene names: 17547431

Gene position: 2917802-2918890 (Clockwise)

Preceding gene: 17547429

Following gene: 17547432

Centisome position: 78.51

GC content: 69.51

Gene sequence:

>1089_bases
ATGTCTGCGCCGCCATTCATGCTGACGCTGTCCGATTTCGATTTCGATCTGCCGCCCGAGCTGATCGCGCAAACCGCCCT
GCCCGACCGCACCGCGAGCCGCCTGCTGGCCGCACGCCACGACGCGCAGGGCACGCATTTCGATGACCGCCAGTTCGCCG
ACCTGGTCGACTACCTGCGCCCCGGCGACCTGCTGGTCTTCAACGACACCCGCGTGATCAAGGCGCGCTTCTTCGGCCAC
AAGGCCAGCGGCGGCAAGATCGAAGTGCTGGTCGAGCGCCTGCTGGACGAGCACACGGTGCTGGCGCAGATCCGCTCCAG
CAAGTCGCCGGTCGCGGGCAGTACGCTGCGCCTGGCCGATGCCTTCGATGTGACGGTCGGACCGCGCCACGAACCCTTCT
TCACCCTGCGCTTCCCGCAGCCGGCGCTGGAGTTGATCGAGCAGTACGGCCGCCTGCCGCTGCCGCCCTATATCGAGCAC
ACGCCCGACAGCTTCGACGAGACGCGCTACCAGACCGTCTATGCGCGCACGCCCGGCGCCGTGGCCGCGCCCACGGCCGG
CCTGCATTTCGACGACGCGCTGTTCGCCAGGCTCGATGCGATGGGCGTGCGGCGCGGCTTCCTGACGCTGCACGTGGGCG
CCGGCACGTTTTCGCCGGTGCGCGTGGAGAACATCGCCGAGCACCGCATGCACAGCGAGTGGTATGCCATCTCGCCGGAC
CTGGCCGAGGCCATCCGCGCCACGCGGGCGGTGGGCGGGCGCATCGTCGCCGTCGGCACCACGTCGATGCGCGCGCTGGA
ATCGGCCGCGCAGCCGGACGGCACGCTGGCGGCCGGCTCGGGCGAGACCGACATCTTCATCACCCCCGGCTACCGCTTCC
GGCTGGTCGACGCGCTGGTGACCAACTTCCACCTGCCCAAGTCGACGCTGCTGATGCTGGTGTCGGCATTCGCGGGCCTG
GAGACCATCCGCGCCGCCTACCGCCACGCCATCGCGCAGCGCTACCGCTTCTTCAGCTACGGCGATGCCATGTTCCTGAC
GCGCGCCGAGCCTGATACCCAGTCCGCTTCCCCCGACCCCTCATGCTGA

Upstream 100 bases:

>100_bases
GGCGGAGCGGGCGCGAGCGGGCACAGGACGTTTACAATGTCGGACTTTGCGGATCGCCGATGATTGTACCGGCGCCCGCC
GCCATCATTTGGCGCAATTC

Downstream 100 bases:

>100_bases
ATTTCGACCTCCTCACCACCGATGGCCTCGCCCGGCGCGGCCGCATGACCCTCAACCACGGCGTGGTGGAAACGCCCATC
TTCATGCCGGTGGGCACCTA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSAPPFMLTLSDFDFDLPPELIAQTALPDRTASRLLAARHDAQGTHFDDRQFADLVDYLRPGDLLVFNDTRVIKARFFGH
KASGGKIEVLVERLLDEHTVLAQIRSSKSPVAGSTLRLADAFDVTVGPRHEPFFTLRFPQPALELIEQYGRLPLPPYIEH
TPDSFDETRYQTVYARTPGAVAAPTAGLHFDDALFARLDAMGVRRGFLTLHVGAGTFSPVRVENIAEHRMHSEWYAISPD
LAEAIRATRAVGGRIVAVGTTSMRALESAAQPDGTLAAGSGETDIFITPGYRFRLVDALVTNFHLPKSTLLMLVSAFAGL
ETIRAAYRHAIAQRYRFFSYGDAMFLTRAEPDTQSASPDPSC

Sequences:

>Translated_362_residues
MSAPPFMLTLSDFDFDLPPELIAQTALPDRTASRLLAARHDAQGTHFDDRQFADLVDYLRPGDLLVFNDTRVIKARFFGH
KASGGKIEVLVERLLDEHTVLAQIRSSKSPVAGSTLRLADAFDVTVGPRHEPFFTLRFPQPALELIEQYGRLPLPPYIEH
TPDSFDETRYQTVYARTPGAVAAPTAGLHFDDALFARLDAMGVRRGFLTLHVGAGTFSPVRVENIAEHRMHSEWYAISPD
LAEAIRATRAVGGRIVAVGTTSMRALESAAQPDGTLAAGSGETDIFITPGYRFRLVDALVTNFHLPKSTLLMLVSAFAGL
ETIRAAYRHAIAQRYRFFSYGDAMFLTRAEPDTQSASPDPSC
>Mature_361_residues
SAPPFMLTLSDFDFDLPPELIAQTALPDRTASRLLAARHDAQGTHFDDRQFADLVDYLRPGDLLVFNDTRVIKARFFGHK
ASGGKIEVLVERLLDEHTVLAQIRSSKSPVAGSTLRLADAFDVTVGPRHEPFFTLRFPQPALELIEQYGRLPLPPYIEHT
PDSFDETRYQTVYARTPGAVAAPTAGLHFDDALFARLDAMGVRRGFLTLHVGAGTFSPVRVENIAEHRMHSEWYAISPDL
AEAIRATRAVGGRIVAVGTTSMRALESAAQPDGTLAAGSGETDIFITPGYRFRLVDALVTNFHLPKSTLLMLVSAFAGLE
TIRAAYRHAIAQRYRFFSYGDAMFLTRAEPDTQSASPDPSC

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=52.3121387283237, Blast_Score=352, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39864; Mature: 39732

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPPFMLTLSDFDFDLPPELIAQTALPDRTASRLLAARHDAQGTHFDDRQFADLVDYLR
CCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
PGDLLVFNDTRVIKARFFGHKASGGKIEVLVERLLDEHTVLAQIRSSKSPVAGSTLRLAD
CCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
AFDVTVGPRHEPFFTLRFPQPALELIEQYGRLPLPPYIEHTPDSFDETRYQTVYARTPGA
EEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
VAAPTAGLHFDDALFARLDAMGVRRGFLTLHVGAGTFSPVRVENIAEHRMHSEWYAISPD
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEECHH
LAEAIRATRAVGGRIVAVGTTSMRALESAAQPDGTLAAGSGETDIFITPGYRFRLVDALV
HHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCCCCEEECCCCCEEEECCCCHHHHHHHHH
TNFHLPKSTLLMLVSAFAGLETIRAAYRHAIAQRYRFFSYGDAMFLTRAEPDTQSASPDP
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
SC
CC
>Mature Secondary Structure 
SAPPFMLTLSDFDFDLPPELIAQTALPDRTASRLLAARHDAQGTHFDDRQFADLVDYLR
CCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
PGDLLVFNDTRVIKARFFGHKASGGKIEVLVERLLDEHTVLAQIRSSKSPVAGSTLRLAD
CCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
AFDVTVGPRHEPFFTLRFPQPALELIEQYGRLPLPPYIEHTPDSFDETRYQTVYARTPGA
EEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
VAAPTAGLHFDDALFARLDAMGVRRGFLTLHVGAGTFSPVRVENIAEHRMHSEWYAISPD
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEECHH
LAEAIRATRAVGGRIVAVGTTSMRALESAAQPDGTLAAGSGETDIFITPGYRFRLVDALV
HHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCCCCEEECCCCCEEEECCCCHHHHHHHHH
TNFHLPKSTLLMLVSAFAGLETIRAAYRHAIAQRYRFFSYGDAMFLTRAEPDTQSASPDP
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
SC
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]