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The map label for this gene is folC [H]

Identifier: 17228521

GI number: 17228521

Start: 1196116

End: 1197369

Strand: Direct

Name: folC [H]

Synonym: alr1026

Alternate gene names: 17228521

Gene position: 1196116-1197369 (Clockwise)

Preceding gene: 17228520

Following gene: 17228523

Centisome position: 18.65

GC content: 40.99

Gene sequence:

>1254_bases
GTGAATATCAATTCCCTACTGCAACCATTTCACCATTTCGGCGTTAACCTCGGACTTTCGCGGATTATCAAATTGCTGGA
CAACCTTGGCAATCCCCATGAGCGAGTTCCCATAATTCATGTAGCTGGCACAAATGGTAAAGGTTCTGTTTGTGCTTATC
TTTCCTCGGTGTTGACTGAGGCTGGTTATCGTACAGGGCGCTACACTTCGCCGCATTTAGTCGATTGGCCAGAGCGTATT
TGCCTGAATGAACAGCAGATTTCTCATGATGAATTGTGTCAATTAGTTTTAACAGTCCAAGCAGCTATTTGTTTTGATGA
TGAATACCCGACTTTATTTGAAGTAATTACCGCCGCCGCTTGGTTATATTTTGCCCAGCAAACAATCGATGTGGCAGTGG
TAGAAGTAGGATTGGGTGGACGGTTGGATGCTACTAATGTAATTGCTGATCCTTTGGTGACAGTGATAACTTCTATCAGT
CGGGAACATTGGCAACAATTGGGGCCGACTGTCGCTGATATTGCTGGGGAAAAAGCGGGGATTCTCAAACCGGGTTGTCC
GGTTGTGGTTGGGACTTTGCCATCGGATGCAGAAAAAGTGGTGCGATCGCGCGCTCAAGAATTACAATGTCCTTTAATTC
TGCCTCAACCTGCTCGTCAAATCTCTCCCCAATTAGCAGAATATCAAAGTATTCAAAACACAAAAACAATTAAATATCCT
CTACCTTTACAAGGACAAATTCAACTCAATAATTCTGCTTTGGCACTAGCAGCTTTAGAAATTTTGCAACAAAAAGGCTG
GCAGATTTCTGAAGCAGAAATTATTAATGGCATGGCAAAAACTAAATGGCCAGGGCGAATGCAATGGATTACTTGGCAAA
ACCACAAATTATTAATTGATGGCGCTCATAATCCGGCGGCGGCTCACGTACTCAGAAACTATGTAGATACCCTAAATAAT
CAATCAGTAACTTGGGTTATGGGAATGTTATCTACGAAAGACCACGCCGATATTTTTCAAGCTTTATTAAGACCAAACGA
CCAATTATATTTAGTTCCAGTACCAGATAATAATTCAGCTAACCCAGAGGTTTTATCCAAGTTAGCTAGTGATGTTTGTC
CAAAATTAAGCACTTGTCAAACTTTTTCAAATTTACCATTAGCCATAGAAACCGCATTCAACTCAACAGATAACTTAGTA
GTGTTATGTGGTTCCTTATATTTAATTGGTCATTTTTTAGCTTCTAAAGGCTAG

Upstream 100 bases:

>100_bases
TCTTTACTGAGACAGTCGGTTTTAATCTGGGGTTGGGGAGATGAGGAAATAAATCAACAAAAACCTTACTACCAATTACC
CATTACCAATTACCCATTCC

Downstream 100 bases:

>100_bases
ATCACAGTAAATTATCCAATTACCAATTACCTATCGACTATTCCACTCCTGTGCCGCATCTTCTACAGCTTTATCTACCG
TTTTTTCTCCCAACATTGCA

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MNINSLLQPFHHFGVNLGLSRIIKLLDNLGNPHERVPIIHVAGTNGKGSVCAYLSSVLTEAGYRTGRYTSPHLVDWPERI
CLNEQQISHDELCQLVLTVQAAICFDDEYPTLFEVITAAAWLYFAQQTIDVAVVEVGLGGRLDATNVIADPLVTVITSIS
REHWQQLGPTVADIAGEKAGILKPGCPVVVGTLPSDAEKVVRSRAQELQCPLILPQPARQISPQLAEYQSIQNTKTIKYP
LPLQGQIQLNNSALALAALEILQQKGWQISEAEIINGMAKTKWPGRMQWITWQNHKLLIDGAHNPAAAHVLRNYVDTLNN
QSVTWVMGMLSTKDHADIFQALLRPNDQLYLVPVPDNNSANPEVLSKLASDVCPKLSTCQTFSNLPLAIETAFNSTDNLV
VLCGSLYLIGHFLASKG

Sequences:

>Translated_417_residues
MNINSLLQPFHHFGVNLGLSRIIKLLDNLGNPHERVPIIHVAGTNGKGSVCAYLSSVLTEAGYRTGRYTSPHLVDWPERI
CLNEQQISHDELCQLVLTVQAAICFDDEYPTLFEVITAAAWLYFAQQTIDVAVVEVGLGGRLDATNVIADPLVTVITSIS
REHWQQLGPTVADIAGEKAGILKPGCPVVVGTLPSDAEKVVRSRAQELQCPLILPQPARQISPQLAEYQSIQNTKTIKYP
LPLQGQIQLNNSALALAALEILQQKGWQISEAEIINGMAKTKWPGRMQWITWQNHKLLIDGAHNPAAAHVLRNYVDTLNN
QSVTWVMGMLSTKDHADIFQALLRPNDQLYLVPVPDNNSANPEVLSKLASDVCPKLSTCQTFSNLPLAIETAFNSTDNLV
VLCGSLYLIGHFLASKG
>Mature_417_residues
MNINSLLQPFHHFGVNLGLSRIIKLLDNLGNPHERVPIIHVAGTNGKGSVCAYLSSVLTEAGYRTGRYTSPHLVDWPERI
CLNEQQISHDELCQLVLTVQAAICFDDEYPTLFEVITAAAWLYFAQQTIDVAVVEVGLGGRLDATNVIADPLVTVITSIS
REHWQQLGPTVADIAGEKAGILKPGCPVVVGTLPSDAEKVVRSRAQELQCPLILPQPARQISPQLAEYQSIQNTKTIKYP
LPLQGQIQLNNSALALAALEILQQKGWQISEAEIINGMAKTKWPGRMQWITWQNHKLLIDGAHNPAAAHVLRNYVDTLNN
QSVTWVMGMLSTKDHADIFQALLRPNDQLYLVPVPDNNSANPEVLSKLASDVCPKLSTCQTFSNLPLAIETAFNSTDNLV
VLCGSLYLIGHFLASKG

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=305, Percent_Identity=34.0983606557377, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI66932984, Length=307, Percent_Identity=33.8762214983713, Blast_Score=149, Evalue=3e-36,
Organism=Escherichia coli, GI1788654, Length=414, Percent_Identity=32.1256038647343, Blast_Score=171, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17553148, Length=438, Percent_Identity=28.310502283105, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71984923, Length=438, Percent_Identity=28.310502283105, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17553150, Length=438, Percent_Identity=28.310502283105, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323760, Length=422, Percent_Identity=35.0710900473934, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6324815, Length=312, Percent_Identity=32.0512820512821, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322718, Length=431, Percent_Identity=25.2900232018561, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24641571, Length=371, Percent_Identity=30.4582210242588, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24581568, Length=190, Percent_Identity=32.1052631578947, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 45724; Mature: 45724

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNINSLLQPFHHFGVNLGLSRIIKLLDNLGNPHERVPIIHVAGTNGKGSVCAYLSSVLTE
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
AGYRTGRYTSPHLVDWPERICLNEQQISHDELCQLVLTVQAAICFDDEYPTLFEVITAAA
CCCCCCCCCCCCCCCCHHHHHCCHHCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
WLYFAQQTIDVAVVEVGLGGRLDATNVIADPLVTVITSISREHWQQLGPTVADIAGEKAG
HHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
ILKPGCPVVVGTLPSDAEKVVRSRAQELQCPLILPQPARQISPQLAEYQSIQNTKTIKYP
CCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEECCCCHHHHCHHHHHHHHHCCCCEEEEC
LPLQGQIQLNNSALALAALEILQQKGWQISEAEIINGMAKTKWPGRMQWITWQNHKLLID
CCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHCCHHHCCCCCCEEEEEECCCEEEEE
GAHNPAAAHVLRNYVDTLNNQSVTWVMGMLSTKDHADIFQALLRPNDQLYLVPVPDNNSA
CCCCHHHHHHHHHHHHHHCCCCEEEHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCC
NPEVLSKLASDVCPKLSTCQTFSNLPLAIETAFNSTDNLVVLCGSLYLIGHFLASKG
CHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNINSLLQPFHHFGVNLGLSRIIKLLDNLGNPHERVPIIHVAGTNGKGSVCAYLSSVLTE
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
AGYRTGRYTSPHLVDWPERICLNEQQISHDELCQLVLTVQAAICFDDEYPTLFEVITAAA
CCCCCCCCCCCCCCCCHHHHHCCHHCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
WLYFAQQTIDVAVVEVGLGGRLDATNVIADPLVTVITSISREHWQQLGPTVADIAGEKAG
HHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
ILKPGCPVVVGTLPSDAEKVVRSRAQELQCPLILPQPARQISPQLAEYQSIQNTKTIKYP
CCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEECCCCHHHHCHHHHHHHHHCCCCEEEEC
LPLQGQIQLNNSALALAALEILQQKGWQISEAEIINGMAKTKWPGRMQWITWQNHKLLID
CCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHCCHHHCCCCCCEEEEEECCCEEEEE
GAHNPAAAHVLRNYVDTLNNQSVTWVMGMLSTKDHADIFQALLRPNDQLYLVPVPDNNSA
CCCCHHHHHHHHHHHHHHCCCCEEEHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCC
NPEVLSKLASDVCPKLSTCQTFSNLPLAIETAFNSTDNLVVLCGSLYLIGHFLASKG
CHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]