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The map label for this gene is nrgA [H]

Identifier: 17228487

GI number: 17228487

Start: 1160381

End: 1161793

Strand: Direct

Name: nrgA [H]

Synonym: alr0992

Alternate gene names: 17228487

Gene position: 1160381-1161793 (Clockwise)

Preceding gene: 17228486

Following gene: 17228491

Centisome position: 18.09

GC content: 48.34

Gene sequence:

>1413_bases
GTGTTTCAGAAAATTTTGATAATTGGGGTGAGTACCCTACTACTGCTGACAAGTCCCATCATGGGTAATGCTTGGGCGCA
AGTCCCAGGGACGACTCCACCTGCTGATACGGGAGATACGGCATTTATGCTGATTTCAGCAGCCCTAGTCTTGCTGATGA
CACCAGGATTAGCATTTTTTTATGGTGGTTTCGTCCGATCGCGCAATATTCTCAATACATTGATGATGAGCTTTGTATTG
ATGGCGATTGTGGGCGTTACCTGGGTTTTATGGGGTTATAGTCTGTCGTTTGCCCCAGGGCTGCCATTTATTGGTGGTTT
ACAGTGGTTTGGGTTAAACGGTGTGGGGCTAGAAACCACAGGTTATCTGCAAGGTTCCGAGCCTGCGAATGTGGTTTCCT
ATGCCGGGACGATTCCCCATCAGGCATACATGATATATCAAGCCATGTTTGCCATTATCACCCCAGCTTTAATTTCTGGT
GCGATCGCCGAACGAATGAGTTTCCGTGCCTATTGCCTATTTGTGCTACTGTGGTCAACCTTTATTTACACGCCTCTAGC
CCACATGGTTTGGGCAAAAGGTGGATTCTTAGGTTTGTATGGCGGCTTAGGTGCGCTCGACTTCGCCGGCGGTACAGTAG
TTCATATTAGCTCTGGGGTTTCTGCCCTCGTCGCCGCAATAGTCCTGGGGCCAAGGAAAACCCATCCCGATCGCCTCAGC
CCTCCCCACAACGTAACATTTATTTTGTTGGGTGCTGGCTTGCTATGGTTTGGCTGGTTCGGTTTTAACGCTGGGAGTGC
CTTATCAGTTGCTAGCAGCACTTCTGGCAGTTTCATCACTAATCCAGCTACAACTGCCTTTGTTGCCACCAACACCTCAG
CCGCAGCCGGAGCTTTAATGTGGCTAATTCTAGAAGGAGTATTACGCGGTAAACCCACCGCCGTAGGTGCAGCTACAGGC
GCAGTTGCCGGTTTAGTAGGTATAACCCCAGCCGCCGGCTTTGTCACCCCTCTAGCGTCAATTTTGATAGGTTTTATCAC
AGCTTTTGTTTGCTTTTATGCCGTAAGTTTCAAACACAAGCTAAATGTTGATGATGCCTTAGATACCTACCCGGTGCATG
GTGTTGGTGGAACAGTTGGCGCAATTCTCACAGCTTTCTTTGCCACCGCCGAAGTTAACTCAGGTGGTAAAAACGGCGTA
CTCAAGGGTAATTTTGGTGAACTAGGTGTGGAACTAGCCGCCATTGTCATCGCCTATGTAATTGCAGCAGTTGGTACTTG
GATAATCTTGAAGATTATTGATGCTACCGTTGGCTTACGGGTGAAAGAAGAAGCAGAATACCAAGGTTTGGATATCAGCG
AACACGGAGAAGAAGCATATAACTCCGAGTTTAGCGATCGCATCGTACCGTAA

Upstream 100 bases:

>100_bases
TAAATCAGAGTAGCTTGCAAGACAAAAATCTTTGGCTAAAAGTAATGAAATGTAACTAAGTATACAAGGTTACATTTCAT
CAGGGAGTGGGAACATAGAC

Downstream 100 bases:

>100_bases
AAACTTAGTCGTAACCCAAAAATGTTTTGACAACTGATACTTTGGGTGACGCTCCTCACATCGCTACCGCTACGCTAAAA
CCAATTGCTTATGGGGAAAC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MFQKILIIGVSTLLLLTSPIMGNAWAQVPGTTPPADTGDTAFMLISAALVLLMTPGLAFFYGGFVRSRNILNTLMMSFVL
MAIVGVTWVLWGYSLSFAPGLPFIGGLQWFGLNGVGLETTGYLQGSEPANVVSYAGTIPHQAYMIYQAMFAIITPALISG
AIAERMSFRAYCLFVLLWSTFIYTPLAHMVWAKGGFLGLYGGLGALDFAGGTVVHISSGVSALVAAIVLGPRKTHPDRLS
PPHNVTFILLGAGLLWFGWFGFNAGSALSVASSTSGSFITNPATTAFVATNTSAAAGALMWLILEGVLRGKPTAVGAATG
AVAGLVGITPAAGFVTPLASILIGFITAFVCFYAVSFKHKLNVDDALDTYPVHGVGGTVGAILTAFFATAEVNSGGKNGV
LKGNFGELGVELAAIVIAYVIAAVGTWIILKIIDATVGLRVKEEAEYQGLDISEHGEEAYNSEFSDRIVP

Sequences:

>Translated_470_residues
MFQKILIIGVSTLLLLTSPIMGNAWAQVPGTTPPADTGDTAFMLISAALVLLMTPGLAFFYGGFVRSRNILNTLMMSFVL
MAIVGVTWVLWGYSLSFAPGLPFIGGLQWFGLNGVGLETTGYLQGSEPANVVSYAGTIPHQAYMIYQAMFAIITPALISG
AIAERMSFRAYCLFVLLWSTFIYTPLAHMVWAKGGFLGLYGGLGALDFAGGTVVHISSGVSALVAAIVLGPRKTHPDRLS
PPHNVTFILLGAGLLWFGWFGFNAGSALSVASSTSGSFITNPATTAFVATNTSAAAGALMWLILEGVLRGKPTAVGAATG
AVAGLVGITPAAGFVTPLASILIGFITAFVCFYAVSFKHKLNVDDALDTYPVHGVGGTVGAILTAFFATAEVNSGGKNGV
LKGNFGELGVELAAIVIAYVIAAVGTWIILKIIDATVGLRVKEEAEYQGLDISEHGEEAYNSEFSDRIVP
>Mature_470_residues
MFQKILIIGVSTLLLLTSPIMGNAWAQVPGTTPPADTGDTAFMLISAALVLLMTPGLAFFYGGFVRSRNILNTLMMSFVL
MAIVGVTWVLWGYSLSFAPGLPFIGGLQWFGLNGVGLETTGYLQGSEPANVVSYAGTIPHQAYMIYQAMFAIITPALISG
AIAERMSFRAYCLFVLLWSTFIYTPLAHMVWAKGGFLGLYGGLGALDFAGGTVVHISSGVSALVAAIVLGPRKTHPDRLS
PPHNVTFILLGAGLLWFGWFGFNAGSALSVASSTSGSFITNPATTAFVATNTSAAAGALMWLILEGVLRGKPTAVGAATG
AVAGLVGITPAAGFVTPLASILIGFITAFVCFYAVSFKHKLNVDDALDTYPVHGVGGTVGAILTAFFATAEVNSGGKNGV
LKGNFGELGVELAAIVIAYVIAAVGTWIILKIIDATVGLRVKEEAEYQGLDISEHGEEAYNSEFSDRIVP

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=431, Percent_Identity=42.6914153132251, Blast_Score=303, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17550182, Length=449, Percent_Identity=29.6213808463252, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17550180, Length=448, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI133931100, Length=438, Percent_Identity=26.7123287671233, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32564042, Length=448, Percent_Identity=25.6696428571429, Blast_Score=112, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324187, Length=452, Percent_Identity=35.6194690265487, Blast_Score=237, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6325396, Length=441, Percent_Identity=35.6009070294785, Blast_Score=232, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6321559, Length=448, Percent_Identity=33.9285714285714, Blast_Score=216, Evalue=6e-57,
Organism=Drosophila melanogaster, GI161078314, Length=431, Percent_Identity=28.7703016241299, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 49114; Mature: 49114

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQKILIIGVSTLLLLTSPIMGNAWAQVPGTTPPADTGDTAFMLISAALVLLMTPGLAFF
CCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
YGGFVRSRNILNTLMMSFVLMAIVGVTWVLWGYSLSFAPGLPFIGGLQWFGLNGVGLETT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCC
GYLQGSEPANVVSYAGTIPHQAYMIYQAMFAIITPALISGAIAERMSFRAYCLFVLLWST
CCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIYTPLAHMVWAKGGFLGLYGGLGALDFAGGTVVHISSGVSALVAAIVLGPRKTHPDRLS
HHHHHHHHHHHHCCCCEEECCCCCCHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCC
PPHNVTFILLGAGLLWFGWFGFNAGSALSVASSTSGSFITNPATTAFVATNTSAAAGALM
CCCCEEEEEEHHHHHHHHHHCCCCCCCEEEECCCCCCEEECCCCEEEEEECCCHHHHHHH
WLILEGVLRGKPTAVGAATGAVAGLVGITPAAGFVTPLASILIGFITAFVCFYAVSFKHK
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LNVDDALDTYPVHGVGGTVGAILTAFFATAEVNSGGKNGVLKGNFGELGVELAAIVIAYV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHH
IAAVGTWIILKIIDATVGLRVKEEAEYQGLDISEHGEEAYNSEFSDRIVP
HHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHCCCHHHCCCCCCCCCC
>Mature Secondary Structure
MFQKILIIGVSTLLLLTSPIMGNAWAQVPGTTPPADTGDTAFMLISAALVLLMTPGLAFF
CCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
YGGFVRSRNILNTLMMSFVLMAIVGVTWVLWGYSLSFAPGLPFIGGLQWFGLNGVGLETT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCC
GYLQGSEPANVVSYAGTIPHQAYMIYQAMFAIITPALISGAIAERMSFRAYCLFVLLWST
CCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIYTPLAHMVWAKGGFLGLYGGLGALDFAGGTVVHISSGVSALVAAIVLGPRKTHPDRLS
HHHHHHHHHHHHCCCCEEECCCCCCHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCC
PPHNVTFILLGAGLLWFGWFGFNAGSALSVASSTSGSFITNPATTAFVATNTSAAAGALM
CCCCEEEEEEHHHHHHHHHHCCCCCCCEEEECCCCCCEEECCCCEEEEEECCCHHHHHHH
WLILEGVLRGKPTAVGAATGAVAGLVGITPAAGFVTPLASILIGFITAFVCFYAVSFKHK
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LNVDDALDTYPVHGVGGTVGAILTAFFATAEVNSGGKNGVLKGNFGELGVELAAIVIAYV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHH
IAAVGTWIILKIIDATVGLRVKEEAEYQGLDISEHGEEAYNSEFSDRIVP
HHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]