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The map label for this gene is amtB [C]

Identifier: 17228486

GI number: 17228486

Start: 1158426

End: 1159982

Strand: Direct

Name: amtB [C]

Synonym: alr0991

Alternate gene names: 17228486

Gene position: 1158426-1159982 (Clockwise)

Preceding gene: 17228485

Following gene: 17228487

Centisome position: 18.06

GC content: 45.47

Gene sequence:

>1557_bases
ATGTACCAACAGAAATCAAGAACAAGAAATAGGCGCTTTTCTACCAGAAATTACGCTAAAAGTAGACAATCTAACTCGCA
AATCCAGATATTCAATCTAGTCAAAAAACTCTCTCCCAGTTGGCAAGCTTGTATACCTCTGGCTTGTTTGATTGTTTTGG
GTTGGAGTTATGTGGCAGTTGCTCAAGCCCCAGCCGCAGGGCCGACAACAGCAGAACTAAAAGTTGCGCTTGACACCCTA
TGGGTAGCGATCGCCGCTTTCTTAGTATTCTTTATGAATGCTGGTTTTGGTATGTTAGAAACCGGCTTCTGTCGTCAGAA
AAATGCTGTTAACGTTCTTGCCAAAAACTTAATCGTGTTCGCCCTGGCAACCGTTGCCTTTTGGGCAATTGGTTTTGGGT
TAATGTTCGGTGATGGCAATGATTTCATTGGCTTCAATGGGTTGTTTCTCTCAGGAGTAGATAACAGCCCGGCAACAGGT
GATGCTTATAAAGGTGTCTTCAGCGCTTTAAGTTGGGCTGGCGTACCTTTAGCCGCAAAATTTTTATTCCAGTTGGTGTT
TGCTGGTACTGCGGCAACAATTGTCTCTGGTGCAGTTGCCGAAAGAATCAAGTTTGTTGACTTCTTGATTTTCAGCCTTT
TACTTGTAGGTATCGCCTACCCGATTACCGGACACTGGATTTGGGGCGCTGGTTGGTTAGCCAAGGCTGGTTTTTGGGAC
TTTGCTGGTTCTACAGTGGTTCACTCAGTTGGCGGTTGGGCAGCTTTGATGGGAGCTGCATTTCTGGGTCCTCGTATTGG
TAAATATCAAGATAAGCAAATTGTGGCTTTACCTGGTCACAACATGAGTATTGCTACCTTGGGCTGCTTGATTCTGTGGT
TAGGTTGGTTTGGTTTCAACCCTGGTTCTGTCATGGCTGCTGACCCCAATGCTATTACTCACATTGCTTTAACAACTAAC
ATGGCTGGTGCCGTTGGTGGTATTGCTGCTACTGCTACCGCTTGGCTGTACTTAGGTAAACCAGACCTGTCAATGATTAT
TAATGGCATTTTGGCTGGTTTAGTTGGTATTACCGCATCTTGCGCTTATGTGAGCATTCCTAGTTCTATTATTATCGGTT
TGATTGCCGGAGTCATAGTAGTTTTCTCTGTGACCTTCTTCGACAAACTAGGTATTGATGACCCAGTAGGAGCAACTTCA
GTTCACCTTGTTTGTGGTGTTTGGGGTACTCTTGCGGTTGGTTTATGGTCGGTTGGCCCTGGTGTTTACTCTTGGTATGG
TGAGGGACTTGGCCCAACCAAGGGTTTATTTGCAGGTGGCGGTTTAGGACAGCTGATTACTCAATTCTTAGGTGCTGCCG
CAGTAGGTGGTATGACTGTACTCGTCAGTAGCATTTTCTGGGTAGTACTGAAAGCTACTTTGGGTATTAGAGTCACCAGA
GAAGAAGAATTGGAAGGCTTGGATATCGGCGAACACGGTATGGAAGCCTACAGTGGATTCCTGAAAGAAGCTAGTCCTGG
TGGATTTGCAGAAGGCAAGACATCTGACGGCTATTAA

Upstream 100 bases:

>100_bases
CACACAAGAACTGATGAGAAAGAGTCTAGAAACTAGGAACTCAGTACTCATTACTTTCTTTTGCTGTTAAAGATAGTTGG
AAGGTTAGCAATTCGGACTC

Downstream 100 bases:

>100_bases
AGGGGTTGATTACAAAACTACCTAATCAATCCTCTATTTAGAATTTATAACCCCGATTTCTCCAAGAAATCGGGGTTTTT
TAATTTAGCTTCCTATATAG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MYQQKSRTRNRRFSTRNYAKSRQSNSQIQIFNLVKKLSPSWQACIPLACLIVLGWSYVAVAQAPAAGPTTAELKVALDTL
WVAIAAFLVFFMNAGFGMLETGFCRQKNAVNVLAKNLIVFALATVAFWAIGFGLMFGDGNDFIGFNGLFLSGVDNSPATG
DAYKGVFSALSWAGVPLAAKFLFQLVFAGTAATIVSGAVAERIKFVDFLIFSLLLVGIAYPITGHWIWGAGWLAKAGFWD
FAGSTVVHSVGGWAALMGAAFLGPRIGKYQDKQIVALPGHNMSIATLGCLILWLGWFGFNPGSVMAADPNAITHIALTTN
MAGAVGGIAATATAWLYLGKPDLSMIINGILAGLVGITASCAYVSIPSSIIIGLIAGVIVVFSVTFFDKLGIDDPVGATS
VHLVCGVWGTLAVGLWSVGPGVYSWYGEGLGPTKGLFAGGGLGQLITQFLGAAAVGGMTVLVSSIFWVVLKATLGIRVTR
EEELEGLDIGEHGMEAYSGFLKEASPGGFAEGKTSDGY

Sequences:

>Translated_518_residues
MYQQKSRTRNRRFSTRNYAKSRQSNSQIQIFNLVKKLSPSWQACIPLACLIVLGWSYVAVAQAPAAGPTTAELKVALDTL
WVAIAAFLVFFMNAGFGMLETGFCRQKNAVNVLAKNLIVFALATVAFWAIGFGLMFGDGNDFIGFNGLFLSGVDNSPATG
DAYKGVFSALSWAGVPLAAKFLFQLVFAGTAATIVSGAVAERIKFVDFLIFSLLLVGIAYPITGHWIWGAGWLAKAGFWD
FAGSTVVHSVGGWAALMGAAFLGPRIGKYQDKQIVALPGHNMSIATLGCLILWLGWFGFNPGSVMAADPNAITHIALTTN
MAGAVGGIAATATAWLYLGKPDLSMIINGILAGLVGITASCAYVSIPSSIIIGLIAGVIVVFSVTFFDKLGIDDPVGATS
VHLVCGVWGTLAVGLWSVGPGVYSWYGEGLGPTKGLFAGGGLGQLITQFLGAAAVGGMTVLVSSIFWVVLKATLGIRVTR
EEELEGLDIGEHGMEAYSGFLKEASPGGFAEGKTSDGY
>Mature_518_residues
MYQQKSRTRNRRFSTRNYAKSRQSNSQIQIFNLVKKLSPSWQACIPLACLIVLGWSYVAVAQAPAAGPTTAELKVALDTL
WVAIAAFLVFFMNAGFGMLETGFCRQKNAVNVLAKNLIVFALATVAFWAIGFGLMFGDGNDFIGFNGLFLSGVDNSPATG
DAYKGVFSALSWAGVPLAAKFLFQLVFAGTAATIVSGAVAERIKFVDFLIFSLLLVGIAYPITGHWIWGAGWLAKAGFWD
FAGSTVVHSVGGWAALMGAAFLGPRIGKYQDKQIVALPGHNMSIATLGCLILWLGWFGFNPGSVMAADPNAITHIALTTN
MAGAVGGIAATATAWLYLGKPDLSMIINGILAGLVGITASCAYVSIPSSIIIGLIAGVIVVFSVTFFDKLGIDDPVGATS
VHLVCGVWGTLAVGLWSVGPGVYSWYGEGLGPTKGLFAGGGLGQLITQFLGAAAVGGMTVLVSSIFWVVLKATLGIRVTR
EEELEGLDIGEHGMEAYSGFLKEASPGGFAEGKTSDGY

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=444, Percent_Identity=33.5585585585586, Blast_Score=194, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI32564042, Length=435, Percent_Identity=34.0229885057471, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17550180, Length=447, Percent_Identity=33.5570469798658, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17550182, Length=438, Percent_Identity=34.4748858447489, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI133931100, Length=441, Percent_Identity=30.1587301587302, Blast_Score=186, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324187, Length=448, Percent_Identity=29.0178571428571, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6321559, Length=448, Percent_Identity=27.2321428571429, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6325396, Length=424, Percent_Identity=27.8301886792453, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI161078314, Length=436, Percent_Identity=38.5321100917431, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 54394; Mature: 54394

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQQKSRTRNRRFSTRNYAKSRQSNSQIQIFNLVKKLSPSWQACIPLACLIVLGWSYVAV
CCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AQAPAAGPTTAELKVALDTLWVAIAAFLVFFMNAGFGMLETGFCRQKNAVNVLAKNLIVF
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHH
ALATVAFWAIGFGLMFGDGNDFIGFNGLFLSGVDNSPATGDAYKGVFSALSWAGVPLAAK
HHHHHHHHHHHHHHEECCCCCEEEECCEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHH
FLFQLVFAGTAATIVSGAVAERIKFVDFLIFSLLLVGIAYPITGHWIWGAGWLAKAGFWD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHCCCHH
FAGSTVVHSVGGWAALMGAAFLGPRIGKYQDKQIVALPGHNMSIATLGCLILWLGWFGFN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCC
PGSVMAADPNAITHIALTTNMAGAVGGIAATATAWLYLGKPDLSMIINGILAGLVGITAS
CCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHH
CAYVSIPSSIIIGLIAGVIVVFSVTFFDKLGIDDPVGATSVHLVCGVWGTLAVGLWSVGP
HHHEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GVYSWYGEGLGPTKGLFAGGGLGQLITQFLGAAAVGGMTVLVSSIFWVVLKATLGIRVTR
CHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEC
EEELEGLDIGEHGMEAYSGFLKEASPGGFAEGKTSDGY
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYQQKSRTRNRRFSTRNYAKSRQSNSQIQIFNLVKKLSPSWQACIPLACLIVLGWSYVAV
CCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AQAPAAGPTTAELKVALDTLWVAIAAFLVFFMNAGFGMLETGFCRQKNAVNVLAKNLIVF
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHH
ALATVAFWAIGFGLMFGDGNDFIGFNGLFLSGVDNSPATGDAYKGVFSALSWAGVPLAAK
HHHHHHHHHHHHHHEECCCCCEEEECCEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHH
FLFQLVFAGTAATIVSGAVAERIKFVDFLIFSLLLVGIAYPITGHWIWGAGWLAKAGFWD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHCCCHH
FAGSTVVHSVGGWAALMGAAFLGPRIGKYQDKQIVALPGHNMSIATLGCLILWLGWFGFN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCC
PGSVMAADPNAITHIALTTNMAGAVGGIAATATAWLYLGKPDLSMIINGILAGLVGITAS
CCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHH
CAYVSIPSSIIIGLIAGVIVVFSVTFFDKLGIDDPVGATSVHLVCGVWGTLAVGLWSVGP
HHHEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GVYSWYGEGLGPTKGLFAGGGLGQLITQFLGAAAVGGMTVLVSSIFWVVLKATLGIRVTR
CHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEC
EEELEGLDIGEHGMEAYSGFLKEASPGGFAEGKTSDGY
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]