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The map label for this gene is amtB [C]

Identifier: 17228485

GI number: 17228485

Start: 1156507

End: 1158003

Strand: Direct

Name: amtB [C]

Synonym: alr0990

Alternate gene names: 17228485

Gene position: 1156507-1158003 (Clockwise)

Preceding gene: 17228484

Following gene: 17228486

Centisome position: 18.03

GC content: 44.49

Gene sequence:

>1497_bases
ATGGCTATAAACACAGGAAAAAATAAGCTGATGAATAGGCATATTCGACCCTGGCAACGCCTATTGGTGTTAGCGATTGG
CTCAATGGTATTTGCTGTTTTTGCACCGACAATTGTGCAAGCGGTAGATACTCCTACCCTAGAATCTTTATCAGAAACTA
CCATCAAACTGCAAATTTCCATTGATACTACTTGGGTATTACTTTCTGGGTTTTTAGTATTTTTCATGCAAACCGGCTTC
GCCATGTTAGAAGCTGGTTTAGTTCGCCAAAGAAGTGTAGTTAACACCCTACTAGAAAATTTCATTGACGCTGCTGTCAC
GGTTTTGGCATGGTGGGCGGTGGGTTTTGGTATCGCATTTGGTACAAGTGCAGGTGGTTTGTTTGGTATAGATACATTCT
TTCTCAGCCAGTTACCGGGTGCTGATGGTAGCTACCCATTAGGCGCACCAGGTTCTACAGCTGCTATTAACACCTATACT
TTGTTCTTTTTCCAGTTTGCCTTTGCTGCTACTGCCAGTACCATTACTACTGGTTCAATGGCTGGCAGAACCGACTTTAT
AGGTGACTTAATTTATAGTGCGATCATGGGTGCTATTAGCTACCCAATTATCGTGCATTGGGCTTGGAACTCCAATGGTT
GGTTAGGCAAACTCAGTTACCATGATTTCGCAGGTGGTTCAATTGTTCATACCGTAGGTGGCTGGACAGCATTAGTTGGG
GCATATTTGCTTGGGCCTCGTCCCGACCGTCCTCCTTGGGGAAAACTACCACCAGCCCATAACTTAGCCTTGGCAACTTT
GGGAACGATGATTCTCTGGTTTGGCTGGTATGGGTTTAACCCTGGTTCAACCCTTGGTACTGCTAATCCGGGGTTGATTG
GGTTAGTAACTATCAACACCACATTAGCAGCAGGTGCTGGCGCACTAGCAGCATTAATCTTTCTGTATGTTCGTACAGGT
AAGTGGGATTTAGTTTATTGTCTCAATGGTTCTCTAGCTGGTTTAGTCGCAATTACAGCACCTTGTGCTTATGTGGCTCC
TTGGGCTTCTGTACTAATTGGTTTAACGGGGGGGATTGCGGTTGTATTAGGAGTGAGTTTAATTGAGTCACTCCACATCG
ACGACCCAGTGGGAGCATTTTCAGTCCACGGTATCAGTGGCATGATGGGTACTCTCTCCATAGGCTTTTTAGGACAAGAG
GAACTCACTCTCAATCAAAAAGCAGGACTATTGCTAGGTGGTGGGTTTGATCTGCTGGGTATCCAAATGCTGGGGATAGT
GGCGATTACGGTTTTCACAGTCGCTTTTGCCTTTCTGATGTATGGTGGCCTCAAAGCTATGGGACACTTACGTGTTAATG
CTGAAGCAGATCGTATTGGTATTGATACTTATGAACATGGTGCTTCAGTGTGGCCTGATGTTTACTCAGTTGAAGAGTTG
AGCAAACCACAAGAACATACAAAAATTTCTGAAAATAAAACTTTAGAGGGGGAATAA

Upstream 100 bases:

>100_bases
AGTTTTCGGGAAACCTCAATAAAAATTTTGACCAAATCTTGTCTACGTTATTTAGTTCACCTAAGCACAAAGGGTAAGTC
TGCTACTACTAACATCAATT

Downstream 100 bases:

>100_bases
AATGGCAAATCTGTTCAATATTTTATCTGACCAATCTTTACAGAAGTTTTTTGGAGAAGATATTGACAATGTGAGGCGAT
CGCTCCTCTAAATCGTCAAA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MAINTGKNKLMNRHIRPWQRLLVLAIGSMVFAVFAPTIVQAVDTPTLESLSETTIKLQISIDTTWVLLSGFLVFFMQTGF
AMLEAGLVRQRSVVNTLLENFIDAAVTVLAWWAVGFGIAFGTSAGGLFGIDTFFLSQLPGADGSYPLGAPGSTAAINTYT
LFFFQFAFAATASTITTGSMAGRTDFIGDLIYSAIMGAISYPIIVHWAWNSNGWLGKLSYHDFAGGSIVHTVGGWTALVG
AYLLGPRPDRPPWGKLPPAHNLALATLGTMILWFGWYGFNPGSTLGTANPGLIGLVTINTTLAAGAGALAALIFLYVRTG
KWDLVYCLNGSLAGLVAITAPCAYVAPWASVLIGLTGGIAVVLGVSLIESLHIDDPVGAFSVHGISGMMGTLSIGFLGQE
ELTLNQKAGLLLGGGFDLLGIQMLGIVAITVFTVAFAFLMYGGLKAMGHLRVNAEADRIGIDTYEHGASVWPDVYSVEEL
SKPQEHTKISENKTLEGE

Sequences:

>Translated_498_residues
MAINTGKNKLMNRHIRPWQRLLVLAIGSMVFAVFAPTIVQAVDTPTLESLSETTIKLQISIDTTWVLLSGFLVFFMQTGF
AMLEAGLVRQRSVVNTLLENFIDAAVTVLAWWAVGFGIAFGTSAGGLFGIDTFFLSQLPGADGSYPLGAPGSTAAINTYT
LFFFQFAFAATASTITTGSMAGRTDFIGDLIYSAIMGAISYPIIVHWAWNSNGWLGKLSYHDFAGGSIVHTVGGWTALVG
AYLLGPRPDRPPWGKLPPAHNLALATLGTMILWFGWYGFNPGSTLGTANPGLIGLVTINTTLAAGAGALAALIFLYVRTG
KWDLVYCLNGSLAGLVAITAPCAYVAPWASVLIGLTGGIAVVLGVSLIESLHIDDPVGAFSVHGISGMMGTLSIGFLGQE
ELTLNQKAGLLLGGGFDLLGIQMLGIVAITVFTVAFAFLMYGGLKAMGHLRVNAEADRIGIDTYEHGASVWPDVYSVEEL
SKPQEHTKISENKTLEGE
>Mature_497_residues
AINTGKNKLMNRHIRPWQRLLVLAIGSMVFAVFAPTIVQAVDTPTLESLSETTIKLQISIDTTWVLLSGFLVFFMQTGFA
MLEAGLVRQRSVVNTLLENFIDAAVTVLAWWAVGFGIAFGTSAGGLFGIDTFFLSQLPGADGSYPLGAPGSTAAINTYTL
FFFQFAFAATASTITTGSMAGRTDFIGDLIYSAIMGAISYPIIVHWAWNSNGWLGKLSYHDFAGGSIVHTVGGWTALVGA
YLLGPRPDRPPWGKLPPAHNLALATLGTMILWFGWYGFNPGSTLGTANPGLIGLVTINTTLAAGAGALAALIFLYVRTGK
WDLVYCLNGSLAGLVAITAPCAYVAPWASVLIGLTGGIAVVLGVSLIESLHIDDPVGAFSVHGISGMMGTLSIGFLGQEE
LTLNQKAGLLLGGGFDLLGIQMLGIVAITVFTVAFAFLMYGGLKAMGHLRVNAEADRIGIDTYEHGASVWPDVYSVEELS
KPQEHTKISENKTLEGE

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=413, Percent_Identity=30.5084745762712, Blast_Score=159, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17550182, Length=441, Percent_Identity=35.1473922902494, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17550180, Length=463, Percent_Identity=31.9654427645788, Blast_Score=241, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI32564042, Length=420, Percent_Identity=32.3809523809524, Blast_Score=208, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI133931100, Length=448, Percent_Identity=31.25, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17563228, Length=242, Percent_Identity=26.8595041322314, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324187, Length=453, Percent_Identity=30.4635761589404, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6321559, Length=455, Percent_Identity=25.9340659340659, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6325396, Length=418, Percent_Identity=27.0334928229665, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI161078314, Length=413, Percent_Identity=35.1089588377724, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI21355411, Length=233, Percent_Identity=28.755364806867, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 52739; Mature: 52608

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINTGKNKLMNRHIRPWQRLLVLAIGSMVFAVFAPTIVQAVDTPTLESLSETTIKLQIS
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEEE
IDTTWVLLSGFLVFFMQTGFAMLEAGLVRQRSVVNTLLENFIDAAVTVLAWWAVGFGIAF
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTSAGGLFGIDTFFLSQLPGADGSYPLGAPGSTAAINTYTLFFFQFAFAATASTITTGSM
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
AGRTDFIGDLIYSAIMGAISYPIIVHWAWNSNGWLGKLSYHDFAGGSIVHTVGGWTALVG
CCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCCCCEEEECCHHHHHHH
AYLLGPRPDRPPWGKLPPAHNLALATLGTMILWFGWYGFNPGSTLGTANPGLIGLVTINT
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECC
TLAAGAGALAALIFLYVRTGKWDLVYCLNGSLAGLVAITAPCAYVAPWASVLIGLTGGIA
HHHCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHCCHHH
VVLGVSLIESLHIDDPVGAFSVHGISGMMGTLSIGFLGQEELTLNQKAGLLLGGGFDLLG
HHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHEEEECCCCCEEECCCCCEEEECCHHHHH
IQMLGIVAITVFTVAFAFLMYGGLKAMGHLRVNAEADRIGIDTYEHGASVWPDVYSVEEL
HHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEECCCCCCCCCCHHCCCCCCCCHHHHHHH
SKPQEHTKISENKTLEGE
CCCHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AINTGKNKLMNRHIRPWQRLLVLAIGSMVFAVFAPTIVQAVDTPTLESLSETTIKLQIS
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEEE
IDTTWVLLSGFLVFFMQTGFAMLEAGLVRQRSVVNTLLENFIDAAVTVLAWWAVGFGIAF
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTSAGGLFGIDTFFLSQLPGADGSYPLGAPGSTAAINTYTLFFFQFAFAATASTITTGSM
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
AGRTDFIGDLIYSAIMGAISYPIIVHWAWNSNGWLGKLSYHDFAGGSIVHTVGGWTALVG
CCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCCCCEEEECCHHHHHHH
AYLLGPRPDRPPWGKLPPAHNLALATLGTMILWFGWYGFNPGSTLGTANPGLIGLVTINT
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECC
TLAAGAGALAALIFLYVRTGKWDLVYCLNGSLAGLVAITAPCAYVAPWASVLIGLTGGIA
HHHCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHCCHHH
VVLGVSLIESLHIDDPVGAFSVHGISGMMGTLSIGFLGQEELTLNQKAGLLLGGGFDLLG
HHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHEEEECCCCCEEECCCCCEEEECCHHHHH
IQMLGIVAITVFTVAFAFLMYGGLKAMGHLRVNAEADRIGIDTYEHGASVWPDVYSVEEL
HHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEECCCCCCCCCCHHCCCCCCCCHHHHHHH
SKPQEHTKISENKTLEGE
CCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]