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The map label for this gene is 218930696

Identifier: 218930696

GI number: 218930696

Start: 4124060

End: 4124518

Strand: Direct

Name: 218930696

Synonym: YPO3689

Alternate gene names: NA

Gene position: 4124060-4124518 (Clockwise)

Preceding gene: 218930695

Following gene: 218930697

Centisome position: 88.62

GC content: 52.94

Gene sequence:

>459_bases
ATGGCCATACTGGGTGCTGTGCTGCTATTGATAGCAGGTGCATGGCAAGGGATTGATGGCGTAACGGTGCGTGATATTGG
TTCCTCGGCAGAGCTTGAGCGCCCGGCGCTTTCGGCCTCTGCCGCTACTGAGTCGTTAACGCAACATCAGAAAGTGGTGA
AATACTTACAAACGCACCATCGGCTACCGGATTTTTACCTGACGAAGAAACAGGCCCGTGAGCAAGGCTGGGATGCCCAA
AAGGGGAACCTCTGTGACGTGTTACCGGGCAAAGCCATTGGCGGTGATCGTTTCTCCAACCGTGAGCGTCAGTTGCCTGA
CGCCAAAGGCCGTCATTGGCGAGAAGCTGATGTGAATTACCGCTGTGGGCGTCGTGGTAGTGATCGCTTGCTGTACTCCA
ATGATGGCCTGATTTATCTGACCCAAGATCACTATAAGCATTTCATTCGGATGGAGTAA

Upstream 100 bases:

>100_bases
GGAATGATATTAATTGTAGGAACGATATTAAGGGCAACAATGAGTAGTACCGCCGTGGTAGGGAGCCTGGGGATTAAGGG
GATCCATGAATAAACGACTG

Downstream 100 bases:

>100_bases
GAAGATGGTAAAGGTGGTATTCGATTTTAACCATATACCGGATCTTCCTGCATTTTACCGCGCATTTTCTCAGAATTTTG
CGCTGAGCGAAGATTTTGGG

Product: putative ribonuclease

Products: NA

Alternate protein names: Barnase; RNase Ba [H]

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MAILGAVLLLIAGAWQGIDGVTVRDIGSSAELERPALSASAATESLTQHQKVVKYLQTHHRLPDFYLTKKQAREQGWDAQ
KGNLCDVLPGKAIGGDRFSNRERQLPDAKGRHWREADVNYRCGRRGSDRLLYSNDGLIYLTQDHYKHFIRME

Sequences:

>Translated_152_residues
MAILGAVLLLIAGAWQGIDGVTVRDIGSSAELERPALSASAATESLTQHQKVVKYLQTHHRLPDFYLTKKQAREQGWDAQ
KGNLCDVLPGKAIGGDRFSNRERQLPDAKGRHWREADVNYRCGRRGSDRLLYSNDGLIYLTQDHYKHFIRME
>Mature_151_residues
AILGAVLLLIAGAWQGIDGVTVRDIGSSAELERPALSASAATESLTQHQKVVKYLQTHHRLPDFYLTKKQAREQGWDAQK
GNLCDVLPGKAIGGDRFSNRERQLPDAKGRHWREADVNYRCGRRGSDRLLYSNDGLIYLTQDHYKHFIRME

Specific function: Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonuclease N1/T1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001887
- InterPro:   IPR000026
- InterPro:   IPR016191 [H]

Pfam domain/function: PF00545 Ribonuclease [H]

EC number: NA

Molecular weight: Translated: 17176; Mature: 17044

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILGAVLLLIAGAWQGIDGVTVRDIGSSAELERPALSASAATESLTQHQKVVKYLQTHH
CHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
RLPDFYLTKKQAREQGWDAQKGNLCDVLPGKAIGGDRFSNRERQLPDAKGRHWREADVNY
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCC
RCGRRGSDRLLYSNDGLIYLTQDHYKHFIRME
CCCCCCCCCEEEECCCEEEEEHHHHHHHHCCC
>Mature Secondary Structure 
AILGAVLLLIAGAWQGIDGVTVRDIGSSAELERPALSASAATESLTQHQKVVKYLQTHH
HHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
RLPDFYLTKKQAREQGWDAQKGNLCDVLPGKAIGGDRFSNRERQLPDAKGRHWREADVNY
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCC
RCGRRGSDRLLYSNDGLIYLTQDHYKHFIRME
CCCCCCCCCEEEECCCEEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3007290; 3050134; 4553460; 1888730; 1569554; 9708913; 2023257; 11914482; 2696173 [H]