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The map label for this gene is pyrI

Identifier: 218930599

GI number: 218930599

Start: 3997521

End: 3997985

Strand: Direct

Name: pyrI

Synonym: YPO3589

Alternate gene names: 218930599

Gene position: 3997521-3997985 (Clockwise)

Preceding gene: 218930598

Following gene: 218930600

Centisome position: 85.9

GC content: 48.39

Gene sequence:

>465_bases
ATGACTCAGGATTACAAACTTCAGGTTGAAGCGATTAAATGCGGCACCGTGATTGACCATATTCCAGCTCAAATCGGCTT
TAAATTGCTCTCACTGTTTAAGCTAACGGCAACCGACCAGCGCATCACCATTGGCTTGAATCTGCCGTCTAAACGTTCTG
GTCGTAAGGATCTGATTAAGATCGAGAACACTTTCCTGACCGAGCAACAAGCGAACCAGTTGGCAATGTATGCTCCAGAT
GCAACCGTCAATCGTATCGATAACTACGAAGTAGTGAAGAAGCTGACCCTCAGCCTGCCAGAGCGTATCGATGCGGTACT
GACGTGCCCAAACAGCAACTGTATCAGCCACAACGAACCCGTCGACTCCAGCTTTACCGTCAAAGCGCAGCGAGGCGAAA
TTAGCCTGAAGTGCAAATATTGTGAGAAAGAGTTCGATCACCTGACCGTACTACACGCGGATTGA

Upstream 100 bases:

>100_bases
ACGCCTACTATTTCCAACAAGCGGGTAACGGTATTTTTGCACGTCAGGCGCTACTGGCACTGGTATTAAACGCAGAATTA
GCTCTTTAAGGGGAATGATG

Downstream 100 bases:

>100_bases
TAACGGTAAGCCCGTAATATTCATCCCAATGGGCAGTGGCTAACTTCGCCCCACGGGTTGCTGTAATTCAGTTCGCCTTT
ATAATGGTCGGGATCATTAT

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MTQDYKLQVEAIKCGTVIDHIPAQIGFKLLSLFKLTATDQRITIGLNLPSKRSGRKDLIKIENTFLTEQQANQLAMYAPD
ATVNRIDNYEVVKKLTLSLPERIDAVLTCPNSNCISHNEPVDSSFTVKAQRGEISLKCKYCEKEFDHLTVLHAD

Sequences:

>Translated_154_residues
MTQDYKLQVEAIKCGTVIDHIPAQIGFKLLSLFKLTATDQRITIGLNLPSKRSGRKDLIKIENTFLTEQQANQLAMYAPD
ATVNRIDNYEVVKKLTLSLPERIDAVLTCPNSNCISHNEPVDSSFTVKAQRGEISLKCKYCEKEFDHLTVLHAD
>Mature_153_residues
TQDYKLQVEAIKCGTVIDHIPAQIGFKLLSLFKLTATDQRITIGLNLPSKRSGRKDLIKIENTFLTEQQANQLAMYAPDA
TVNRIDNYEVVKKLTLSLPERIDAVLTCPNSNCISHNEPVDSSFTVKAQRGEISLKCKYCEKEFDHLTVLHAD

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=151, Percent_Identity=78.8079470198676, Blast_Score=242, Evalue=9e-66,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_YERPA (Q1C1J6)

Other databases:

- EMBL:   CP000308
- RefSeq:   YP_653621.1
- ProteinModelPortal:   Q1C1J6
- SMR:   Q1C1J6
- STRING:   Q1C1J6
- GeneID:   4118274
- GenomeReviews:   CP000308_GR
- KEGG:   ypa:YPA_3714
- eggNOG:   COG1781
- HOGENOM:   HBG305327
- OMA:   EGTVIDH
- ProtClustDB:   PRK00893
- BioCyc:   YPES360102:YPA_3714-MONOMER
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 17337; Mature: 17206

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQDYKLQVEAIKCGTVIDHIPAQIGFKLLSLFKLTATDQRITIGLNLPSKRSGRKDLIK
CCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEE
IENTFLTEQQANQLAMYAPDATVNRIDNYEVVKKLTLSLPERIDAVLTCPNSNCISHNEP
ECCEEECCCCCCEEEEECCCCHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCC
VDSSFTVKAQRGEISLKCKYCEKEFDHLTVLHAD
CCCCEEEEEECCCEEEEEEECCCCCCEEEEEECC
>Mature Secondary Structure 
TQDYKLQVEAIKCGTVIDHIPAQIGFKLLSLFKLTATDQRITIGLNLPSKRSGRKDLIK
CCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEE
IENTFLTEQQANQLAMYAPDATVNRIDNYEVVKKLTLSLPERIDAVLTCPNSNCISHNEP
ECCEEECCCCCCEEEEECCCCHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCC
VDSSFTVKAQRGEISLKCKYCEKEFDHLTVLHAD
CCCCEEEEEECCCEEEEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA