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The map label for this gene is gltD [H]

Identifier: 218930570

GI number: 218930570

Start: 3971610

End: 3973028

Strand: Direct

Name: gltD [H]

Synonym: YPO3558

Alternate gene names: 218930570

Gene position: 3971610-3973028 (Clockwise)

Preceding gene: 218930569

Following gene: 218930571

Centisome position: 85.34

GC content: 51.37

Gene sequence:

>1419_bases
ATGAGTCAGAACGTTTATCAGTTTATCGACTTACAGCGCGTCGATCCGCCTAAAAAACCGCTGAAGATCCGTAAAATTGA
ATTTGTTGAAATTTACGAGCCATTCTCAGAAACACAAGCCAAAGCACAGGCCGATCGCTGCCTGTCCTGCGGCAATCCGT
ACTGTGAATGGAAATGCCCGGTTCATAACTACATCCCTAACTGGCTGAAACTGGCCAACGAGGGGCGGATTATGGAAGCG
GCTGATCTGGCACACCAAACGAACAGCCTACCGGAGGTCTGTGGCCGTGTTTGTCCACAAGATCGCTTGTGTGAAGGATC
TTGTACTCTGAACGACGAATTTGGTGCCGTGACCATCGGCAACATCGAGCGTTATATCAGTGACAAAGCCATTGCGATGG
GCTGGAAACCGGATATGTCACATGTTCACCCTACCGGTAAACGTGTGGCCGTGATCGGGGCCGGCCCTGCCGGGCTGGCC
TGTGCTGATGTATTAGCACGTAACGGTGTTCAGGCTGTGGTCTTTGACCGCCATCCAGAAATTGGGGGGTTACTGACCTT
CGGTATCCCAGCGTTTAAGCTGGAAAAAGAAGTGATGATTAAACGCCGTAAGATCTTCTCCGAGATGGGAATTGAATTCC
AACTGAATACTGAAGTAGGCAAAGACATCACCATGGAAGCACTGCTGAAAGATTACGACGCCGTGTTCCTGGGGGTTGGG
ACCTACCAATCTATGCGTGGTGGTTTGGAAAACGAAGAGGCTAACGGTGTATATGATGCCTTGCCTTTCCTGATTGCTAA
TACTAAACAGCTAATGGGCTATGAAGCGACGGCTCACGAACCGTATATCAACATGCAAGGTAAACGCGTCGTGGTGCTTG
GTGGCGGTGATACCGCAATGGACTGTGTGCGCTCCTCAATTCGTCAGGGGGCAACCGATGTGGTTTGCGCCTATCGCCGC
GATGAAGTGAATATGCCGGGTTCCAAGCGGGAAGTGAAAAACGCCCGTGAAGAAGGGGTAGAATTTAAATTCAACCTGCA
ACCATTAAGCATTGAAGTGAACAGCAATGGCAAAGTCTGTGGTGTCAGAATGGTCCGCACCCAGTTGGGCGCGCCAGATG
CACAGGGCCGGCGCATGGCTGAACAGATCCCTGGCTCAGAACATGTATTGCCAGCAGATGCCGTAGTGATGGCATTTGGT
TTCCGCCCACACAGTATGGAATGGCTGGCCGCTCACGATGTGAAGCTGGATAAGCAAGGCCGGGTGATTGCACCGGAAAG
CACTGATAATGCCTTCCAGACCAGTAACCCGAAAATCTTTGCGGGCGGCGATATTGTCCGTGGTTCCGATTTAGTGGTTA
CCGCCATTGCCGAAGGCCGTAAAGCCGCAGACGGCATCATGAATTATCTGGAAGTCTAA

Upstream 100 bases:

>100_bases
TGCCTTGGTTAAACCGAAATCCAGCGATGTGAAAGCACTGTTGGGCCACCGTAGCCGTTCCGCAGCTGAGCTGCGGGTTC
AAGCGCAGTAAGGAGTTGAT

Downstream 100 bases:

>100_bases
TGCGGTAGATCCCCAAATCATTCAATAAACGCTAGGGCGGGTTCAATTCACAGAACCCGCCCTTTCTATTTATTCTATTT
ATTTATGCACGTCTATTGTT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSETQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIMEA
ADLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYISDKAIAMGWKPDMSHVHPTGKRVAVIGAGPAGLA
CADVLARNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMIKRRKIFSEMGIEFQLNTEVGKDITMEALLKDYDAVFLGVG
TYQSMRGGLENEEANGVYDALPFLIANTKQLMGYEATAHEPYINMQGKRVVVLGGGDTAMDCVRSSIRQGATDVVCAYRR
DEVNMPGSKREVKNAREEGVEFKFNLQPLSIEVNSNGKVCGVRMVRTQLGAPDAQGRRMAEQIPGSEHVLPADAVVMAFG
FRPHSMEWLAAHDVKLDKQGRVIAPESTDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV

Sequences:

>Translated_472_residues
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSETQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIMEA
ADLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYISDKAIAMGWKPDMSHVHPTGKRVAVIGAGPAGLA
CADVLARNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMIKRRKIFSEMGIEFQLNTEVGKDITMEALLKDYDAVFLGVG
TYQSMRGGLENEEANGVYDALPFLIANTKQLMGYEATAHEPYINMQGKRVVVLGGGDTAMDCVRSSIRQGATDVVCAYRR
DEVNMPGSKREVKNAREEGVEFKFNLQPLSIEVNSNGKVCGVRMVRTQLGAPDAQGRRMAEQIPGSEHVLPADAVVMAFG
FRPHSMEWLAAHDVKLDKQGRVIAPESTDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV
>Mature_471_residues
SQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSETQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIMEAA
DLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYISDKAIAMGWKPDMSHVHPTGKRVAVIGAGPAGLAC
ADVLARNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMIKRRKIFSEMGIEFQLNTEVGKDITMEALLKDYDAVFLGVGT
YQSMRGGLENEEANGVYDALPFLIANTKQLMGYEATAHEPYINMQGKRVVVLGGGDTAMDCVRSSIRQGATDVVCAYRRD
EVNMPGSKREVKNAREEGVEFKFNLQPLSIEVNSNGKVCGVRMVRTQLGAPDAQGRRMAEQIPGSEHVLPADAVVMAFGF
RPHSMEWLAAHDVKLDKQGRVIAPESTDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=483, Percent_Identity=29.6066252587992, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI1789606, Length=472, Percent_Identity=84.5338983050847, Blast_Score=853, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=461, Percent_Identity=65.29284164859, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI87082180, Length=450, Percent_Identity=57.5555555555556, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1788468, Length=442, Percent_Identity=29.185520361991, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789243, Length=422, Percent_Identity=31.9905213270142, Blast_Score=151, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17570289, Length=489, Percent_Identity=31.0838445807771, Blast_Score=221, Evalue=9e-58,
Organism=Caenorhabditis elegans, GI71984108, Length=468, Percent_Identity=31.4102564102564, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17543860, Length=161, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=467, Percent_Identity=34.475374732334, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI28574881, Length=486, Percent_Identity=37.6543209876543, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24665539, Length=486, Percent_Identity=37.6543209876543, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24665547, Length=486, Percent_Identity=37.6543209876543, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24665543, Length=486, Percent_Identity=37.6543209876543, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24640763, Length=466, Percent_Identity=29.1845493562232, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI18858217, Length=466, Percent_Identity=29.1845493562232, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51943; Mature: 51812

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSETQAKAQADRCLSCGNPYCEWKCP
CCCCHHHHHHHHCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIMEAADLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIG
HHHHCCHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC
NIERYISDKAIAMGWKPDMSHVHPTGKRVAVIGAGPAGLACADVLARNGVQAVVFDRHPE
CHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMIKRRKIFSEMGIEFQLNTEVGKDITMEALLKDYDAVFLGVG
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCEEEEECC
TYQSMRGGLENEEANGVYDALPFLIANTKQLMGYEATAHEPYINMQGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCEEECCCCEEEEECCCCHHH
DCVRSSIRQGATDVVCAYRRDEVNMPGSKREVKNAREEGVEFKFNLQPLSIEVNSNGKVC
HHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEEEECCCCCEE
GVRMVRTQLGAPDAQGRRMAEQIPGSEHVLPADAVVMAFGFRPHSMEWLAAHDVKLDKQG
EEEEEEHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEECEEECCCC
RVIAPESTDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV
CEECCCCCCCCEECCCCEEEECCCEEECCCEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSETQAKAQADRCLSCGNPYCEWKCP
CCCHHHHHHHHCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIMEAADLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIG
HHHHCCHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC
NIERYISDKAIAMGWKPDMSHVHPTGKRVAVIGAGPAGLACADVLARNGVQAVVFDRHPE
CHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMIKRRKIFSEMGIEFQLNTEVGKDITMEALLKDYDAVFLGVG
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCEEEEECC
TYQSMRGGLENEEANGVYDALPFLIANTKQLMGYEATAHEPYINMQGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCEEECCCCEEEEECCCCHHH
DCVRSSIRQGATDVVCAYRRDEVNMPGSKREVKNAREEGVEFKFNLQPLSIEVNSNGKVC
HHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEEEECCCCCEE
GVRMVRTQLGAPDAQGRRMAEQIPGSEHVLPADAVVMAFGFRPHSMEWLAAHDVKLDKQG
EEEEEEHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEECEEECCCC
RVIAPESTDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV
CEECCCCCCCCEECCCCEEEECCCEEECCCEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]