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The map label for this gene is tolA [H]

Identifier: 218928291

GI number: 218928291

Start: 1268903

End: 1270069

Strand: Direct

Name: tolA [H]

Synonym: YPO1123

Alternate gene names: 218928291

Gene position: 1268903-1270069 (Clockwise)

Preceding gene: 218928290

Following gene: 218928292

Centisome position: 27.27

GC content: 50.81

Gene sequence:

>1167_bases
GTGGGTAAGGCAACCGAGCAAAATGATAAGCTGAATCGCGCCGTTATTGTTTCGGTGGTTCTGCACATCATATTGATTGC
CCTGTTGATTTGGGGCTCTCTGACGCAGACGACTGAAATGGGCGGTGGCGGTGCTGGCGGCGAAGTGATTGACGCTGTAA
TGGTCGATCCGGGTGCGGTGACAGAGCAATATAATCGTCAACAGCAACAGCAAACAGATGCTAAACGTGCCGAGCAGCAG
CGTCAGAAAAAAGCTGAGCAGCAAGCCGAAGAATTGCAGCAAAAGCAGGCTGCTGAACAACAGCGGCTGAAAGAATTAGA
AAAAGAGCGTCTGCAAGCGCAAGAAGATGCGAAGCTGGCTGCTGAAGAGCAGAAAAAGCAAGTTGCTGAACAACAAAAAC
AAATCGCTGAACAGCAAAAGCAGGCCGCTGAACAACAAAAAATTGCTGCTGCTGCCGTTGCAAAAGCGAAAGAAGAGCAG
AAGCAGGCTGAAACGGCTGCGGCTCAGGCAAAAGCTGAAGCAGATAAAATAGTGAAAGCGCAGGCTGAAGCACAGAAAAA
AGCCGAGGCTGAAGCCAAAAAAGAAGCTGCTGTTGCCGCTGCGGCGAAGAAACAAGCCGATGCCGATGCGAAGAAAGCGG
TAGAGGTTGCCGAAAAGGCTGCGGCTGACGCAGCAGAAAAGAAAGCGGCCGCAGATGCAGAGAAAAAAGCCGCTGCTGCT
AAAAAAGTTGCTGCCGCCGCTGAGGCCAAGAAAAAAGCGGCAGCAGAAGCGGCTGCCTCAACGGATGTTGATGATTTGTT
TGGTGGTTTGGCAAACGCTAAAAATGCACCGAAGAGTGGTTCGGGTGCAGGTGCCGCTGCTGCCGGTAAAGGTGGCGGTA
AGAAAAGCGGGGCATCAGGTGCTGATATCAGCGGCTACCTGGGGCAAATCACCGGCGCGATACAAAGTAAATTTTATGAT
GCAGATCTTTACAAAGGCCGTACCTGTGATTTGCGAATCAAATTGGCACCAGACGGTCTATTAATCGATGTGAAAGCAGA
AGGGGGGGACCCGGCACTGTGCCAGGCTGCTATCGCTGCGGCAAAACAAGCGAAAATTCCTAAGCCTCCAAGTACGGATG
TCTATGAACAATTTAAGAATGCTCCGCTTGTGTTTAAGCCTCAATAA

Upstream 100 bases:

>100_bases
TATTGGTTGGGCTAATCGTTAGCTCAACCCGTAGATACCCTGTTTTTAGCAGTTCCGTTTTTTAGCAATAGAGCGCGGAC
ATCGAGTGTGGAAATCGAGC

Downstream 100 bases:

>100_bases
GGTTTGATTGACAGGGTGTAACGTTGTTAGTTAATGATGGATCATGTAGTTTTGTTTGCGGTGGTTTGTTAAAATTCTGC
TAATTATCGCGGGCATCCCG

Product: cell envelope integrity inner membrane protein TolA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MGKATEQNDKLNRAVIVSVVLHIILIALLIWGSLTQTTEMGGGGAGGEVIDAVMVDPGAVTEQYNRQQQQQTDAKRAEQQ
RQKKAEQQAEELQQKQAAEQQRLKELEKERLQAQEDAKLAAEEQKKQVAEQQKQIAEQQKQAAEQQKIAAAAVAKAKEEQ
KQAETAAAQAKAEADKIVKAQAEAQKKAEAEAKKEAAVAAAAKKQADADAKKAVEVAEKAAADAAEKKAAADAEKKAAAA
KKVAAAAEAKKKAAAEAAASTDVDDLFGGLANAKNAPKSGSGAGAAAAGKGGGKKSGASGADISGYLGQITGAIQSKFYD
ADLYKGRTCDLRIKLAPDGLLIDVKAEGGDPALCQAAIAAAKQAKIPKPPSTDVYEQFKNAPLVFKPQ

Sequences:

>Translated_388_residues
MGKATEQNDKLNRAVIVSVVLHIILIALLIWGSLTQTTEMGGGGAGGEVIDAVMVDPGAVTEQYNRQQQQQTDAKRAEQQ
RQKKAEQQAEELQQKQAAEQQRLKELEKERLQAQEDAKLAAEEQKKQVAEQQKQIAEQQKQAAEQQKIAAAAVAKAKEEQ
KQAETAAAQAKAEADKIVKAQAEAQKKAEAEAKKEAAVAAAAKKQADADAKKAVEVAEKAAADAAEKKAAADAEKKAAAA
KKVAAAAEAKKKAAAEAAASTDVDDLFGGLANAKNAPKSGSGAGAAAAGKGGGKKSGASGADISGYLGQITGAIQSKFYD
ADLYKGRTCDLRIKLAPDGLLIDVKAEGGDPALCQAAIAAAKQAKIPKPPSTDVYEQFKNAPLVFKPQ
>Mature_387_residues
GKATEQNDKLNRAVIVSVVLHIILIALLIWGSLTQTTEMGGGGAGGEVIDAVMVDPGAVTEQYNRQQQQQTDAKRAEQQR
QKKAEQQAEELQQKQAAEQQRLKELEKERLQAQEDAKLAAEEQKKQVAEQQKQIAEQQKQAAEQQKIAAAAVAKAKEEQK
QAETAAAQAKAEADKIVKAQAEAQKKAEAEAKKEAAVAAAAKKQADADAKKAVEVAEKAAADAAEKKAAADAEKKAAAAK
KVAAAAEAKKKAAAEAAASTDVDDLFGGLANAKNAPKSGSGAGAAAAGKGGGKKSGASGADISGYLGQITGAIQSKFYDA
DLYKGRTCDLRIKLAPDGLLIDVKAEGGDPALCQAAIAAAKQAKIPKPPSTDVYEQFKNAPLVFKPQ

Specific function: Involved in the tonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA [H]

COG id: COG3064

COG function: function code M; Membrane protein involved in colicin uptake

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786960, Length=125, Percent_Identity=68.8, Blast_Score=170, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014161 [H]

Pfam domain/function: PF06519 TolA [H]

EC number: NA

Molecular weight: Translated: 40425; Mature: 40294

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKATEQNDKLNRAVIVSVVLHIILIALLIWGSLTQTTEMGGGGAGGEVIDAVMVDPGAV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHCCCCHH
TEQYNRQQQQQTDAKRAEQQRQKKAEQQAEELQQKQAAEQQRLKELEKERLQAQEDAKLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEEQKKQVAEQQKQIAEQQKQAAEQQKIAAAAVAKAKEEQKQAETAAAQAKAEADKIVKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAEAQKKAEAEAKKEAAVAAAAKKQADADAKKAVEVAEKAAADAAEKKAAADAEKKAAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKVAAAAEAKKKAAAEAAASTDVDDLFGGLANAKNAPKSGSGAGAAAAGKGGGKKSGASG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADISGYLGQITGAIQSKFYDADLYKGRTCDLRIKLAPDGLLIDVKAEGGDPALCQAAIAA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHH
AKQAKIPKPPSTDVYEQFKNAPLVFKPQ
HHHCCCCCCCCCHHHHHHCCCCCEECCC
>Mature Secondary Structure 
GKATEQNDKLNRAVIVSVVLHIILIALLIWGSLTQTTEMGGGGAGGEVIDAVMVDPGAV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHCCCCHH
TEQYNRQQQQQTDAKRAEQQRQKKAEQQAEELQQKQAAEQQRLKELEKERLQAQEDAKLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEEQKKQVAEQQKQIAEQQKQAAEQQKIAAAAVAKAKEEQKQAETAAAQAKAEADKIVKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAEAQKKAEAEAKKEAAVAAAAKKQADADAKKAVEVAEKAAADAAEKKAAADAEKKAAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKVAAAAEAKKKAAAEAAASTDVDDLFGGLANAKNAPKSGSGAGAAAAGKGGGKKSGASG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADISGYLGQITGAIQSKFYDADLYKGRTCDLRIKLAPDGLLIDVKAEGGDPALCQAAIAA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHH
AKQAKIPKPPSTDVYEQFKNAPLVFKPQ
HHHCCCCCCCCCHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2687247; 8905232; 9278503; 2068069; 8978668; 10404600 [H]