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The map label for this gene is csdA [H]

Identifier: 218928196

GI number: 218928196

Start: 1168058

End: 1169377

Strand: Reverse

Name: csdA [H]

Synonym: YPO1028

Alternate gene names: 218928196

Gene position: 1169377-1168058 (Counterclockwise)

Preceding gene: 218928200

Following gene: 218928195

Centisome position: 25.13

GC content: 46.52

Gene sequence:

>1320_bases
GTGCGACCATCACTAATGTGTATAACGTCAATATGCATAACATCATTGCTCAAACACGCGACCATGATAAAAGCAAAAAA
ATTTCGGGGTCACTCACAGGATGCCAACAACAGTATGAAAGTTTTTAATCCAATGGATTTTCGTCGGGAATTCCCTGCGC
TCAGTGATAAATTAACCTATCTGGACAGTGCGGCGACCGCCTTGAAACCACGTGCAATGATTGACGCGACACAGCAATTT
TATCAGCAGGATTCAGCAACGGTACACCGCAGCCAACATCAATCGGCGCTGTCATTAACGGTTCGCTTTGAAAACACCCG
CCAACAAGTGGCTGATTTTATTAACTCATCTACAGCAGAAAATATTATCTGGACGCGAGGAACAACTGAAGCCATCAATC
TGATCGCGCAAAGTTATGCCCGCCCCCGTTTACAACCTGAAGATGAAATTATTGTCAGCGAAGCTGAACATCATGCGAAT
TTAATTCCCTGGTTGATGGTAGCGGAGCAGACCGGTGCAAAAATAGTCAAATTACCTCTTGGCCTTGATCATCTGCCAGA
TTTACAGCAACTCCCTCAACTACTTAATGAAAAAACACGCATATTAGCGCTGGGGCAGATGTCTAACGTAACAGGCGGTA
GCCCTGATCTGGCTCAGGCTATTAGGCTGGCTCACCAATATGACTGTGTTGTCGTGGTTGACGGTGCTCAGGGAATTGTT
CATTACCCAGCCGATGTTCAGGCATTGGATATTGATTTTTATGCATTCTCTTCCCATAAATTGTATGGCCCAACCGGCAT
TGGCGTGCTGTATGGGAAGACTGAATTATTAGAAGAGATGCCCGCCTGGCAAGGCGGCGGTAAAATGCTTACCCATGCAT
CATTCGGGGGCTTTACACCTCATGAAGTGCCTTATCGCTTTGAAGCGGGTACACCCAATATTGCTGGCGTTATTGGTTTA
TCAGCGGTACTCAAATGGCTGGAACATATTGATCTGGAAGAGGCCGAAGTTTATAGCCAAGGTTTAGCTACAATGGCAGA
AAATAAGCTCGCACAATTACCGGGTTTTCACAGTTACCGTTGTCAGCAATCCAGTTTATTAGCATTTACTTTCGATGGTG
TTCATCACAGTGATTTAGTGGCGTTATTGGCCGAGCAAGGTATCGCACTACGTGCTGGGCAGCACTGCGCACAGCCACTG
ATGGCGGCTCTGGGAGTCAATGGCAGTCTGCGGGCTTCTTTTGCGCCTTATAATACCCCCCAAGATGTTGAAATGCTTTG
CTCTGCGCTTGGTAAGGCATTGGAACTGCTTCGAGACTAA

Upstream 100 bases:

>100_bases
TACGTCTGATCGATTTACCTATTCTCGGCCCCCATTCACACAGGGGTGACTGATCATCTCTATCTCATCGTCTACTTTCG
GTTTACCATCAGTAACGATA

Downstream 100 bases:

>100_bases
CTGCTGCCAATGATAATTTTCGATATACCCTAAATGATTCCAGTTGCAAGAGAGCAGCAATCAAGTGAACGAAAGCCATC
AACACAAGCCAGCTTGAAGA

Product: cysteine sulfinate desulfinase

Products: @ALAN01txt*L-Alanine! [C]

Alternate protein names: CSD [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MRPSLMCITSICITSLLKHATMIKAKKFRGHSQDANNSMKVFNPMDFRREFPALSDKLTYLDSAATALKPRAMIDATQQF
YQQDSATVHRSQHQSALSLTVRFENTRQQVADFINSSTAENIIWTRGTTEAINLIAQSYARPRLQPEDEIIVSEAEHHAN
LIPWLMVAEQTGAKIVKLPLGLDHLPDLQQLPQLLNEKTRILALGQMSNVTGGSPDLAQAIRLAHQYDCVVVVDGAQGIV
HYPADVQALDIDFYAFSSHKLYGPTGIGVLYGKTELLEEMPAWQGGGKMLTHASFGGFTPHEVPYRFEAGTPNIAGVIGL
SAVLKWLEHIDLEEAEVYSQGLATMAENKLAQLPGFHSYRCQQSSLLAFTFDGVHHSDLVALLAEQGIALRAGQHCAQPL
MAALGVNGSLRASFAPYNTPQDVEMLCSALGKALELLRD

Sequences:

>Translated_439_residues
MRPSLMCITSICITSLLKHATMIKAKKFRGHSQDANNSMKVFNPMDFRREFPALSDKLTYLDSAATALKPRAMIDATQQF
YQQDSATVHRSQHQSALSLTVRFENTRQQVADFINSSTAENIIWTRGTTEAINLIAQSYARPRLQPEDEIIVSEAEHHAN
LIPWLMVAEQTGAKIVKLPLGLDHLPDLQQLPQLLNEKTRILALGQMSNVTGGSPDLAQAIRLAHQYDCVVVVDGAQGIV
HYPADVQALDIDFYAFSSHKLYGPTGIGVLYGKTELLEEMPAWQGGGKMLTHASFGGFTPHEVPYRFEAGTPNIAGVIGL
SAVLKWLEHIDLEEAEVYSQGLATMAENKLAQLPGFHSYRCQQSSLLAFTFDGVHHSDLVALLAEQGIALRAGQHCAQPL
MAALGVNGSLRASFAPYNTPQDVEMLCSALGKALELLRD
>Mature_439_residues
MRPSLMCITSICITSLLKHATMIKAKKFRGHSQDANNSMKVFNPMDFRREFPALSDKLTYLDSAATALKPRAMIDATQQF
YQQDSATVHRSQHQSALSLTVRFENTRQQVADFINSSTAENIIWTRGTTEAINLIAQSYARPRLQPEDEIIVSEAEHHAN
LIPWLMVAEQTGAKIVKLPLGLDHLPDLQQLPQLLNEKTRILALGQMSNVTGGSPDLAQAIRLAHQYDCVVVVDGAQGIV
HYPADVQALDIDFYAFSSHKLYGPTGIGVLYGKTELLEEMPAWQGGGKMLTHASFGGFTPHEVPYRFEAGTPNIAGVIGL
SAVLKWLEHIDLEEAEVYSQGLATMAENKLAQLPGFHSYRCQQSSLLAFTFDGVHHSDLVALLAEQGIALRAGQHCAQPL
MAALGVNGSLRASFAPYNTPQDVEMLCSALGKALELLRD

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine. L-cysteine sulfinic acid is the best substrate. Functions as a selenium delivery protein in the pathway f

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=399, Percent_Identity=24.3107769423559, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1789175, Length=401, Percent_Identity=67.0822942643392, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI1787970, Length=404, Percent_Identity=45.049504950495, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI48994898, Length=406, Percent_Identity=25.8620689655172, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI25143064, Length=395, Percent_Identity=25.3164556962025, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI193211090, Length=390, Percent_Identity=25.3846153846154, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319831, Length=398, Percent_Identity=24.3718592964824, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129463, Length=393, Percent_Identity=25.4452926208651, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR022471
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 4.4.1.- [C]

Molecular weight: Translated: 48190; Mature: 48190

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSLMCITSICITSLLKHATMIKAKKFRGHSQDANNSMKVFNPMDFRREFPALSDKLTY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHCCCHHHHHHH
LDSAATALKPRAMIDATQQFYQQDSATVHRSQHQSALSLTVRFENTRQQVADFINSSTAE
HHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEEECHHHHHHHHHHCCCCCC
NIIWTRGTTEAINLIAQSYARPRLQPEDEIIVSEAEHHANLIPWLMVAEQTGAKIVKLPL
CEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEECHHHHCCCCHHHEEECCCCCEEEEECC
GLDHLPDLQQLPQLLNEKTRILALGQMSNVTGGSPDLAQAIRLAHQYDCVVVVDGAQGIV
CCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCEE
HYPADVQALDIDFYAFSSHKLYGPTGIGVLYGKTELLEEMPAWQGGGKMLTHASFGGFTP
ECCCCCEEEEEEEEEECCCEEECCCCCEEEECCHHHHHHCCCCCCCCCEEEECCCCCCCC
HEVPYRFEAGTPNIAGVIGLSAVLKWLEHIDLEEAEVYSQGLATMAENKLAQLPGFHSYR
CCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
CQQSSLLAFTFDGVHHSDLVALLAEQGIALRAGQHCAQPLMAALGVNGSLRASFAPYNTP
CCCCCEEEEEECCCCHHHHHHHHHHCCCEECCCHHHHHHHHHHHCCCCCCEECCCCCCCC
QDVEMLCSALGKALELLRD
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSLMCITSICITSLLKHATMIKAKKFRGHSQDANNSMKVFNPMDFRREFPALSDKLTY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHCCCHHHHHHH
LDSAATALKPRAMIDATQQFYQQDSATVHRSQHQSALSLTVRFENTRQQVADFINSSTAE
HHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEEECHHHHHHHHHHCCCCCC
NIIWTRGTTEAINLIAQSYARPRLQPEDEIIVSEAEHHANLIPWLMVAEQTGAKIVKLPL
CEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEECHHHHCCCCHHHEEECCCCCEEEEECC
GLDHLPDLQQLPQLLNEKTRILALGQMSNVTGGSPDLAQAIRLAHQYDCVVVVDGAQGIV
CCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCEE
HYPADVQALDIDFYAFSSHKLYGPTGIGVLYGKTELLEEMPAWQGGGKMLTHASFGGFTP
ECCCCCEEEEEEEEEECCCEEECCCCCEEEECCHHHHHHCCCCCCCCCEEEECCCCCCCC
HEVPYRFEAGTPNIAGVIGLSAVLKWLEHIDLEEAEVYSQGLATMAENKLAQLPGFHSYR
CCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
CQQSSLLAFTFDGVHHSDLVALLAEQGIALRAGQHCAQPLMAALGVNGSLRASFAPYNTP
CCCCCEEEEEECCCCHHHHHHHHHHCCCEECCCHHHHHHHHHHHCCCCCCEECCCCCCCC
QDVEMLCSALGKALELLRD
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cystine; L-selenocystine [C]

Specific reaction: Catalyzes the removal of elemental sulfur and selenium atoms from l-cysteine, l-cystine, l-selenocysteine, and l- selenocystine to produce @ALAN01.txt*l-Alanine! [C]

General reaction: Remove sulfur and selenium atoms [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278392; 9278503; 10829016; 10739946 [H]