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The map label for this gene is top6A

Identifier: 15921561

GI number: 15921561

Start: 1295004

End: 1296167

Strand: Direct

Name: top6A

Synonym: ST1295

Alternate gene names: 15921561

Gene position: 1295004-1296167 (Clockwise)

Preceding gene: 15921560

Following gene: 15921562

Centisome position: 48.06

GC content: 32.99

Gene sequence:

>1164_bases
ATGAGTAGTGAATTAACTTCTAAGGTGGATAAAGAAGCTAGGAAGAAGGCAGCTGACACTCTTAGGCAAGTTTTTGTAAA
ATTAATTGAGCAAATAAATAACTCTGAACCTCCAACTATGGAAATTCCAAAGAGAACTCTTGGCAATACTATTTATGATG
AAAAGAGAAAGCTACTTCTTTTAGGCGAAGAGAAATTAAAGAGAAGTTTCTTCGATCTTAATGAGTCTAAAAGATTTATG
CAAACAGTACTTATGGCTAGTATAATATACGATGCACTTATTAACGATGAATACCCAACAATACGTGATCTATATTATAG
AGGTAAACATTCTATTATATTAAAGGATCCTAGAGGTAAAACTTATGAAGAAAATACATGGGACGAGCAAAAGGAGTCAG
ATAGTGTAATAATGGATATTGAGGTATTTACGTCTTTGCTTAGGGAAGATATGCTTATTCTAAGTAAGGAGAAAGGTAAA
GTTGTCGGTGATATGAGAATCAGAAGTGGAAACGACATAATTGATTTAAGCAAAATGGGTCATGGTGCATATTCTATTGA
GCCAACCCCAGATTTAATAGATTTTGTAGATATTAATGCTGAATTTGTATTAGTAGTTGAGAAAGATGCTGTATTTCAAC
AGCTTCATAGGGCAGGATTCTGGAAACAATATAAAGCCATTTTAGTTACAAGTGCAGGACAGCCAGATAGAGCGACAAGA
AGATTCGTAAGAAGACTGAATGAAGAACTAAAATTACCGGTTTATATTTTAACCGATGCAGATCCATACGGATGGTATAT
TTATAGTGTTTTTAGAATAGGATCTATTTCGTTATCATACGAAAGTGAAAGACTTGCTACTCCTAATGCGAAGTTCCTTG
GAGTCTCAATGACTGATATTTTTGGCGATAGTAATAAGAAACCGTATCTATCAGAGCAAGAAAGAAGAAACTATATTATA
AAAGCCAAAGATGCTGATATAAAAAGAGCTACTGAAATAAAGAACTATCAATGGTTTAAGACTAAGGCATGGCAACATGA
AATAGAGATCTTTCTTAATAAAAAGTCTAAGTTAGAAATAGAAGCTATGGCGAGTAAGGGATTAAAATTCTTAGCGTTTC
AATACATTCCAGAGAAAATTAAATCAAAAGATTATATTGAGTGA

Upstream 100 bases:

>100_bases
CAGATTTGAGAGAAAAACTACTTAAAATAGCGTTGAATAAACTTGAGGTAAACGATAAAAAATTAGAGGAGGAAATACGT
AATTATAAAGTAGAGGAGTT

Downstream 100 bases:

>100_bases
TCCGCCGAGACAGGTTATTTCATCATTCAAAAATTGGGGTCTCTTCATCATTCATAGTATCCTAGGATATCATTAAAAGT
ATTTCTTTTGCATACTCTAC

Product: DNA topoisomerase VI subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSSELTSKVDKEARKKAADTLRQVFVKLIEQINNSEPPTMEIPKRTLGNTIYDEKRKLLLLGEEKLKRSFFDLNESKRFM
QTVLMASIIYDALINDEYPTIRDLYYRGKHSIILKDPRGKTYEENTWDEQKESDSVIMDIEVFTSLLREDMLILSKEKGK
VVGDMRIRSGNDIIDLSKMGHGAYSIEPTPDLIDFVDINAEFVLVVEKDAVFQQLHRAGFWKQYKAILVTSAGQPDRATR
RFVRRLNEELKLPVYILTDADPYGWYIYSVFRIGSISLSYESERLATPNAKFLGVSMTDIFGDSNKKPYLSEQERRNYII
KAKDADIKRATEIKNYQWFKTKAWQHEIEIFLNKKSKLEIEAMASKGLKFLAFQYIPEKIKSKDYIE

Sequences:

>Translated_387_residues
MSSELTSKVDKEARKKAADTLRQVFVKLIEQINNSEPPTMEIPKRTLGNTIYDEKRKLLLLGEEKLKRSFFDLNESKRFM
QTVLMASIIYDALINDEYPTIRDLYYRGKHSIILKDPRGKTYEENTWDEQKESDSVIMDIEVFTSLLREDMLILSKEKGK
VVGDMRIRSGNDIIDLSKMGHGAYSIEPTPDLIDFVDINAEFVLVVEKDAVFQQLHRAGFWKQYKAILVTSAGQPDRATR
RFVRRLNEELKLPVYILTDADPYGWYIYSVFRIGSISLSYESERLATPNAKFLGVSMTDIFGDSNKKPYLSEQERRNYII
KAKDADIKRATEIKNYQWFKTKAWQHEIEIFLNKKSKLEIEAMASKGLKFLAFQYIPEKIKSKDYIE
>Mature_386_residues
SSELTSKVDKEARKKAADTLRQVFVKLIEQINNSEPPTMEIPKRTLGNTIYDEKRKLLLLGEEKLKRSFFDLNESKRFMQ
TVLMASIIYDALINDEYPTIRDLYYRGKHSIILKDPRGKTYEENTWDEQKESDSVIMDIEVFTSLLREDMLILSKEKGKV
VGDMRIRSGNDIIDLSKMGHGAYSIEPTPDLIDFVDINAEFVLVVEKDAVFQQLHRAGFWKQYKAILVTSAGQPDRATRR
FVRRLNEELKLPVYILTDADPYGWYIYSVFRIGSISLSYESERLATPNAKFLGVSMTDIFGDSNKKPYLSEQERRNYIIK
AKDADIKRATEIKNYQWFKTKAWQHEIEIFLNKKSKLEIEAMASKGLKFLAFQYIPEKIKSKDYIE

Specific function: Relaxes both positive and negative superturns and exhibits a strong decatenase activity

COG id: COG1697

COG function: function code L; DNA topoisomerase VI, subunit A

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TOP6A family

Homologues:

Organism=Homo sapiens, GI6912680, Length=370, Percent_Identity=26.7567567567568, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI38201680, Length=313, Percent_Identity=28.7539936102236, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17542064, Length=214, Percent_Identity=31.7757009345794, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45552753, Length=356, Percent_Identity=28.6516853932584, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP6A_SULTO (Q971T1)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377230.1
- ProteinModelPortal:   Q971T1
- SMR:   Q971T1
- GeneID:   1459297
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1295
- NMPDR:   fig|273063.1.peg.1398
- HOGENOM:   HBG559107
- OMA:   DGDPYGW
- ProtClustDB:   PRK04342
- BioCyc:   STOK273063:ST1295-MONOMER
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- HAMAP:   MF_00132
- InterPro:   IPR002815
- InterPro:   IPR013049
- InterPro:   IPR004085
- InterPro:   IPR022904
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10848
- PRINTS:   PR01550
- PRINTS:   PR01552

Pfam domain/function: PF04406 TP6A_N; SSF56726 Spo11/TopoVI_A

EC number: =5.99.1.3

Molecular weight: Translated: 45087; Mature: 44956

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: ACT_SITE 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSELTSKVDKEARKKAADTLRQVFVKLIEQINNSEPPTMEIPKRTLGNTIYDEKRKLLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHCCCEEEE
LGEEKLKRSFFDLNESKRFMQTVLMASIIYDALINDEYPTIRDLYYRGKHSIILKDPRGK
ECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCC
TYEENTWDEQKESDSVIMDIEVFTSLLREDMLILSKEKGKVVGDMRIRSGNDIIDLSKMG
CCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCEEEHHHCC
HGAYSIEPTPDLIDFVDINAEFVLVVEKDAVFQQLHRAGFWKQYKAILVTSAGQPDRATR
CCEEECCCCCCCEEEEECCCEEEEEEECHHHHHHHHHCCHHHHEEEEEEECCCCCCHHHH
RFVRRLNEELKLPVYILTDADPYGWYIYSVFRIGSISLSYESERLATPNAKFLGVSMTDI
HHHHHHHHHHCCCEEEEECCCCCCCHHEEHHHHCEEEEEECCCCCCCCCCEEEEEEHHHH
FGDSNKKPYLSEQERRNYIIKAKDADIKRATEIKNYQWFKTKAWQHEIEIFLNKKSKLEI
CCCCCCCCCCCCHHHCCEEEEECCCCHHHHHHCCCCEEEEHHHHHEEEEEEECCCCCEEH
EAMASKGLKFLAFQYIPEKIKSKDYIE
HHHHHCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSELTSKVDKEARKKAADTLRQVFVKLIEQINNSEPPTMEIPKRTLGNTIYDEKRKLLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHCCCEEEE
LGEEKLKRSFFDLNESKRFMQTVLMASIIYDALINDEYPTIRDLYYRGKHSIILKDPRGK
ECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCC
TYEENTWDEQKESDSVIMDIEVFTSLLREDMLILSKEKGKVVGDMRIRSGNDIIDLSKMG
CCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCEEEHHHCC
HGAYSIEPTPDLIDFVDINAEFVLVVEKDAVFQQLHRAGFWKQYKAILVTSAGQPDRATR
CCEEECCCCCCCEEEEECCCEEEEEEECHHHHHHHHHCCHHHHEEEEEEECCCCCCHHHH
RFVRRLNEELKLPVYILTDADPYGWYIYSVFRIGSISLSYESERLATPNAKFLGVSMTDI
HHHHHHHHHHCCCEEEEECCCCCCCHHEEHHHHCEEEEEECCCCCCCCCCEEEEEEHHHH
FGDSNKKPYLSEQERRNYIIKAKDADIKRATEIKNYQWFKTKAWQHEIEIFLNKKSKLEI
CCCCCCCCCCCCHHHCCEEEEECCCCHHHHHHCCCCEEEEHHHHHEEEEEEECCCCCEEH
EAMASKGLKFLAFQYIPEKIKSKDYIE
HHHHHCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479